BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,559,935	Δ1 bp	100%	intergenic (‑211/+59)	ispZ ← / ← ftsY	intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,559,935	0	C→.	100.0%	42.9	125	intergenic (‑211/+59)	ispZ/ftsY	intracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand): new base (104/6): ref base (0/15): total (104/21)
Fisher's exact test strand distribution p-value = 5.99e-15
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.92e-06

Only 100 of 179 total aligned reads displayed.


CGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGGGGGGGGCAGCGCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCCTC  >  NC_014034/2559835‑2560035


cGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAgcgc                                                                                                         >  1:1636607/1‑101 (MQ=255)


 ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcc                                                                                                        >  2:142104/1‑101 (MQ=255)


 ggTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcc                                                                                                        >  2:2253207/1‑101 (MQ=255)


   tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcccc                                                                                                      >  1:4684694/1‑101 (MQ=255)


   tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGcccc                                                                                                      >  2:3151549/1‑101 (MQ=255)


       cTGACAGGATTGTTTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGCCA.GCGCAAGCGGGGGGCAGCGccccccga                                                                                                  <  2:2854532/101‑3 (MQ=255)


       cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccga                                                                                                  >  2:2475159/1‑99 (MQ=255)


       cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccga                                                                                                  >  1:1871173/1‑99 (MQ=255)


        tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaa                                                                                                 >  1:3673363/1‑98 (MQ=255)


        tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaa                                                                                                 >  2:3518619/1‑98 (MQ=255)


        tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaa                                                                                                 >  2:1451011/1‑98 (MQ=255)


         gACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCGccccccgaag                                                                                                >  1:2096426/1‑97 (MQ=255)


           cAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAgcg                                                                                          >  2:4627301/1‑101 (MQ=255)


               aTTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTg                                                                                      >  1:1968095/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAg                                                                                    >  1:755574/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAg                                                                                    >  1:3236455/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAg                                                                                    >  1:620316/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAg                                                                                    >  1:4712962/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAg                                                                                    >  2:4390084/1‑101 (MQ=255)


                 tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAg                                                                                    >  1:4421197/1‑101 (MQ=255)


                  ttgtCCTGCGCCCCGGAGCGGCCCCTTTTTTCCGGGGGCAAAAC.GGCGGGGGGTT.GCGCGGCC.GCGGAAGGGGGGGGCCCCCCCCCCCC.G..AAGCGCCTGAg                                                                                    <  2:1825014/100‑1 (MQ=255)


                  gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAgg                                                                                   >  1:2725848/1‑101 (MQ=255)


                  gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGCCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAgg                                                                                   >  2:4663860/1‑101 (MQ=255)


                  gtgtCCTCCGCCCCGCAGCAGGCCCGTTTTTCCTTGGGCGAACC.GCGGGGGGGTT.GCGCGTCA.GCGCAAGGGGGGGGCAGC.CCCCCCC.G..AAGCGCCTGAgg                                                                                   <  2:2831972/101‑1 (MQ=255)


                    gtCCTTCGCGCCAGGGCGGCCCCCTTTTTCCTGGCGCGACGC.GCCGGGGCGTT.GCCCTTCG.GCCAAAGCGGGGGGC.GCCGCCCCCC.G..AAGCGCCTGAGgcg                                                                                 <  2:700449/101‑1 (MQ=255)


                     tCCTTGGCGCGGCAGCAGCCCCTCTTTTCCTTGGGCAAAAC.GCGGGGGGGTG.GCGCTTCA.GCGCAAGCGGGGGGCAGC.CCCCCCC.G..AAGCGCCTGAGgcgc                                                                                <  2:1520000/101‑1 (MQ=255)


                      ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..ACGCGCCTGAGGcgcg                                                                               >  2:3774413/1‑101 (MQ=255)


                      ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGcgcg                                                                               >  1:2635951/1‑101 (MQ=255)


                       cTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCgcgg                                                                              >  2:2104864/1‑101 (MQ=255)


                        ttCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCgcggc                                                                             >  1:2560805/1‑101 (MQ=255)


                            cgccgcAGCGGCCCCGCTTTTCCTTGGGCAAACC.GCGGGGGGTTT.GCGGGTCC.GCGCAGGGGGGGGGCGGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGc                                                                         <  1:2370778/101‑1 (MQ=255)


                            cgccgcAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAACGCCTGAGGCGCGGCGGGc                                                                         >  1:2669014/1‑101 (MQ=255)


                            cgccgcAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGAAAGGGGGGGGCAGC.CCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGc                                                                         <  2:2806209/101‑1 (MQ=255)


                             gccgcAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCCCGGCGGGCt                                                                        >  2:732903/1‑101 (MQ=255)


                                gcagcaGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTgc                                                                     >  1:3509113/1‑101 (MQ=255)


                                gcagcaGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTgc                                                                     >  2:2021750/1‑101 (MQ=255)


                                cccggcggCCCCTTTTTCCTGGGGGGAAAC.GGGGGGGGGGT.GCGGCGCG.GCCGAAGGGGGGGGGCGCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTg                                                                      <  1:2685097/95‑1 (MQ=255)


                                 gagccagCCCGCTTTTCTGTGGGGGAAAC.CGGGGGGGGTTCGCGCGGCG.GCGCGGGGGGGGGCCCGC.CCCCCCG.G..AAGCGCCTGAGGCGCGGCGGGCTTgc                                                                     <  2:1149216/95‑1 (MQ=255)


                                 cagcagGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTgcc                                                                    >  2:799794/1‑101 (MQ=255)


                                  agcagGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTgccc                                                                   >  2:1787428/1‑101 (MQ=255)


                                    ggggCCCGTTTTTCC.TGGGGGAAACGGCGGGGGGGTG.GCGGGGCG.GCGACGGGGGGGGGCAGC.CCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                                 <  1:4487960/99‑1 (MQ=12)


                                    cagGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCCTgcccgc                                                                 >  2:52177/1‑101 (MQ=255)


                                     agGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCttatccccc                                                                >  1:4581701/1‑94 (MQ=255)


                                     agGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTgcccgcc                                                                >  1:4755342/1‑101 (MQ=255)


                                          cgtttttCCGGGGGCGAACC.GCGGGGGGGTT.GCGCGTCG.GCG.AAGGGGGGGGGCGCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACg                                                           <  1:3877034/98‑1 (MQ=21)


                                           ttttttCCTGGGGGAAAAC.CCGGGGGGGGG.GCCCGCCA..CGCAAGGGGGGGGCCCCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGa                                                          <  1:3116689/99‑1 (MQ=16)


                                             ttttCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAg                                                        >  1:4315061/1‑101 (MQ=255)


                                              tttCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CTCCCC.G..AAGCGCCTGAGGCGCGGCGGGCCTGCCCGCCCCACGCAgg                                                       >  2:2637905/1‑101 (MQ=255)


                                              tttCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCGTGCCCGCCCAACGAAgg                                                       >  2:3193282/1‑101 (MQ=255)


                                              tttCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCCTgaccgccccaaaaccg                                                       >  2:719425/1‑93 (MQ=255)


                                                  cTTGGGCGAAAC.GCCGGGGGGTT.GCTCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCATGGCCGCCCAACGAAGGCGCa                                                   >  1:1429203/1‑101 (MQ=255)


                                                   ttGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCaa                                                  >  1:1525981/1‑101 (MQ=255)


                                                    ggggCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAac                                                 <  1:3156886/100‑1 (MQ=255)


                                                         gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAAGCGCAACCCCCt                                            >  2:3336501/1‑101 (MQ=25)


                                                         gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGGAGGCGCAACCACCt                                            >  2:679215/1‑101 (MQ=255)


                                                         gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                            >  2:4047606/1‑101 (MQ=255)


                                                         gAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                            >  1:100222/1‑101 (MQ=255)


                                                          agcc.GCGGGGGGGTG.GCGCGGCG.GCGAAGGGGGGGGGCGGC.CCCCCCG.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                                           <  1:560774/98‑1 (MQ=18)


                                                          aaaC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACCAAGGCGCAACCACCtt                                           >  1:3422257/1‑101 (MQ=255)


                                                           aaC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTc                                          >  2:27473/1‑101 (MQ=255)


                                                           aaC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCCTTCCCGCCCCACCAACGAGCAACCACCCTc                                          >  1:2379909/1‑101 (MQ=255)


                                                            aC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                                         >  2:3008746/1‑101 (MQ=255)


                                                              gggaggggggGT.GCGCGGCAGGCGAAGGGGGGGCGCA.CCCCCCCCC.A..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                                         <  1:3823768/97‑1 (MQ=18)


                                                                 cGGGGGGTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACTAAGGCGCAACCACCTTCAGTCg                                     >  1:4157067/1‑101 (MQ=255)


                                                                  ggggggTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCCGTCgg                                    >  2:3091122/1‑101 (MQ=255)


                                                                  ggggggGG.GGGCGGCG.GCGGAAGGGGGGGGCCCCCCCCCCCG.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                                     <  1:352065/101‑1 (MQ=21)


                                                                   gggggTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGGAGGCCGCACCGCCTTCAggccgc                                   >  2:4223771/1‑96 (MQ=21)


                                                                       gcgcgcgcGTCG.GCGC.GGGGGGGGGCCCCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTc                                <  1:1309876/97‑1 (MQ=21)


                                                                       gTT.GCGCGTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCCCCCAACGAAGGCGCAACCACCCTCAGTCGGCTTCg                               >  1:1687746/1‑101 (MQ=25)


                                                                         t.GCGCGCCG.GCG.AAGGGGGGGGCCGGCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt                             <  1:2475302/101‑1 (MQ=25)


                                                                            cgcgTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcc                           >  2:240148/1‑101 (MQ=255)


                                                                               gTCA.GCGCAAGCGGGGGGCAGCGCCCCCCC....AAGCGCCTGGGGCGCGGCGGGCCTCCCCGCCCAACGAAGGCGCACCCACCATCAGCCGGTTTAGATccccc                        >  2:3967988/1‑100 (MQ=255)


                                                                               gTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca                        >  1:4284615/1‑101 (MQ=255)


                                                                               gTCA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca                        >  2:623235/1‑101 (MQ=255)


                                                                                tCA.GCGCAAGCGGGGCGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTTCCCGCCCAACGAAGGCGCAACCACCTTCCGGCGGGTTCGATcccccc                       >  2:1245083/1‑99 (MQ=255)


                                                                                 cA.GCGCAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc                      >  2:514782/1‑101 (MQ=255)


                                                                                  ggcgccgaGGGGGGGGGCCCGCCCCCCCGG..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca                        <  2:4152799/94‑1 (MQ=25)


                                                                                  ccgggcggggggggggGCGCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac                       <  1:4627554/93‑1 (MQ=25)


                                                                                   gccgcggggggggggCAGC.CCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca                     <  1:3897725/94‑1 (MQ=25)


                                                                                    cgcgagggggggggCCCCCCCCCCCG.A..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca                     <  1:4269622/95‑1 (MQ=25)


                                                                                     gggagggggggggCCCCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAg                    <  2:170788/96‑1 (MQ=37)


                                                                                      gcAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCCGTCGGCTTCGATCCCCACCAgcc                  >  2:4662358/1‑100 (MQ=25)


                                                                                      gcAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcc                  >  1:1478213/1‑100 (MQ=255)


                                                                                       cAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCCCCAgcgc                 >  2:2677176/1‑101 (MQ=255)


                                                                                       cAAGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAAGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCCgcgc                 >  2:4515819/1‑101 (MQ=25)


                                                                                        aaGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg                >  2:4159688/1‑101 (MQ=255)


                                                                                        aaGCGGGGGGCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCAGCATCGATACCCACCAGcgcg                >  2:3769674/1‑101 (MQ=21)


                                                                                         gggggggggCCCCCCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg                <  1:1103892/100‑1 (MQ=255)


                                                                                         agcggggggCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCCCCCGCgcgg               >  1:1769935/4‑101 (MQ=25)


                                                                                         agcggggggCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcgg               >  1:185183/4‑101 (MQ=255)


                                                                                          gcggggggCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggc              >  1:2853476/3‑101 (MQ=255)


                                                                                            ggggggCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcgg            >  1:4349519/1‑101 (MQ=255)


                                                                                            ggggggCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcgg            >  1:3069906/1‑101 (MQ=255)


                                                                                              ggggCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggcg          >  1:2925874/1‑101 (MQ=255)


                                                                                                ggcccccccccccc.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGa         <  1:327867/94‑1 (MQ=255)


                                                                                                 gCAGCG.CCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGGGAAt       >  1:443903/1‑101 (MQ=255)


                                                                                                  gcagcccccccc.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAAt       <  1:2910987/96‑1 (MQ=255)


                                                                                                  cccccccccccg.GA.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGaa        <  1:2393807/96‑1 (MQ=37)


                                                                                                    cccccccccc.GAAAAGCGCCTGAGGCGCGGCGGGCTTCCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAAt       <  1:3311741/98‑1 (MQ=255)


                                                                                                       ccccccg.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCCCCTTCAGTCGGCTGCGATCCCCACCAGCGCGGCGGCGAATTCCTc  <  1:4211553/101‑1 (MQ=255)


CGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAAC.GCCGGGGGGTT.GCGCGTCA.GCGCAAGGGGGGGGCAGCGCCCCCCC.G..AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCCTC  >  NC_014034/2559835‑2560035

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 23 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG