BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,617,778	Δ1 bp	100%	intergenic (‑152/‑462)	RCAP_rcc02439 ← / → RCAP_rcc02440	hypothetical protein/AsnC/Lrp family transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,617,778	0	C→.	100.0%	22.2	165	intergenic (‑152/‑462)	RCAP_rcc02439/RCAP_rcc02440	hypothetical protein/AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand): new base (94/61): ref base (2/8): total (96/69)
Fisher's exact test strand distribution p-value = 1.77e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.27e-06

Only 100 of 215 total aligned reads displayed.


GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAG  >  NC_014034/2617678‑2617877


  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccc                                                                                                   <  1:4256493/101‑1 (MQ=255)


     gATCAAGGCCGGAATTCGTGCAGCCGACACAGCGCAGTTTACAGCCGCTGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCTCGGCTcccccc                                                                                                <  2:3160363/101‑1 (MQ=255)


     gATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccc                                                                                                <  2:3775589/101‑1 (MQ=255)


     gATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccc                                                                                                <  1:455353/101‑1 (MQ=255)


      aTCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGCACAGCGACAGCGGCCTCCCGGCTccccccc                                                                                               <  1:4220964/101‑1 (MQ=255)


       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                              >  2:525667/1‑101 (MQ=255)


        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                             >  2:208444/1‑100 (MQ=255)


        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                             >  2:1664158/1‑100 (MQ=255)


         aaGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                            >  1:4383464/1‑99 (MQ=255)


         aaGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgc                                                                                            >  2:650121/1‑99 (MQ=255)


         aaGGCAGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                            >  2:897358/1‑99 (MQ=255)


         aaGCCCGGAATTCGGGCAGCCGACACAGCGCAGCGGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                            <  1:2099673/101‑3 (MQ=255)


          aGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggc                                                                                           <  2:3618865/101‑4 (MQ=255)


           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                          <  2:2146190/101‑5 (MQ=255)


            gCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCa                                                                                        <  1:4177687/101‑1 (MQ=255)


            gCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCa                                                                                        <  1:3703731/101‑1 (MQ=255)


             ccGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                       <  2:1275370/101‑1 (MQ=255)


             ccGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                       <  2:3832143/101‑1 (MQ=255)


             ccGGAATTCGGCCAGCCGCCACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                       <  2:1032698/101‑1 (MQ=255)


              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCTGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                      <  2:3934542/101‑1 (MQ=255)


              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggccgaa                                                                                       >  2:3999393/1‑94 (MQ=255)


               ggAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAg                                                                                     >  2:3760510/1‑101 (MQ=255)


               ggAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAg                                                                                     <  1:2279971/101‑1 (MQ=255)


                gAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGc                                                                                    >  2:1321134/1‑101 (MQ=255)


                 aaTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcc                                                                                   <  2:788608/101‑1 (MQ=255)


                  aTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGccc                                                                                  <  2:4324946/101‑1 (MQ=255)


                     cGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCgg                                                                               >  1:929257/1‑101 (MQ=255)


                     cGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCgg                                                                               >  1:4651882/1‑101 (MQ=255)


                      gggCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGc                                                                              >  2:1145793/1‑101 (MQ=255)


                         cAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCg                                                                           >  1:604555/1‑101 (MQ=255)


                            cagtcccaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGagt                                                                        >  2:4673614/7‑99 (MQ=255)


                              gACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCg                                                                      >  2:1941784/1‑101 (MQ=255)


                              gACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCg                                                                      >  1:3115062/1‑101 (MQ=255)


                               acacAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGt                                                                     >  2:304272/1‑101 (MQ=255)


                               acacAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGt                                                                     <  2:88850/101‑1 (MQ=255)


                                 acaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                   >  2:3337720/1‑101 (MQ=255)


                                 acaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                   >  2:1209298/1‑101 (MQ=255)


                                 acaGCGCAGCGGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                   <  1:4452908/101‑1 (MQ=255)


                                   aGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGt                                                                 <  2:2530781/101‑1 (MQ=255)


                                   aGCGCAGCTGACAGCCGCCGACGATGTCGAAACGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGt                                                                 >  1:1079023/1‑101 (MQ=255)


                                    gcgcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTc                                                                <  1:1833329/101‑1 (MQ=255)


                                      gcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcgc                                                              <  2:2876411/101‑1 (MQ=255)


                                        aGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGCCTGTCgcgg                                                            >  1:505264/1‑101 (MQ=255)


                                           tGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCCAAGCCCCCGCCCGACTCGTCTGTCGCGGCGa                                                         >  2:1555154/1‑101 (MQ=255)


                                               aGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGa                                                     <  1:2372226/101‑1 (MQ=255)


                                                  cgccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgt                                                  >  2:1393190/1‑101 (MQ=255)


                                                  cgccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgt                                                  >  2:3666413/1‑101 (MQ=255)


                                                   gccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgtg                                                 >  1:2989212/1‑101 (MQ=255)


                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                              >  2:1613626/1‑101 (MQ=255)


                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGCCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGCTGTGTCa                                              >  1:2214363/1‑101 (MQ=255)


                                                      aacagtGTCGAAATGCAGCGTGGCCAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                              <  2:251200/96‑1 (MQ=255)


                                                        cgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGGCATg                                            >  1:240066/1‑101 (MQ=255)


                                                           tGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGc                                         >  1:867942/1‑101 (MQ=255)


                                                              cGAAATGCAGCGTGGGCGGGGACGGGGCCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGCCTCGTGTGTCGCGGCGACGGATGTGTCATGGGCttt                                      <  1:1718558/101‑1 (MQ=255)


                                                               gAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                     <  2:3017192/101‑1 (MQ=255)


                                                               gAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                     <  2:4194978/101‑1 (MQ=255)


                                                               gAAAGGCATGGTGGAAGGCGCCAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                     <  2:2010419/101‑1 (MQ=255)


                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                    <  2:1351620/101‑1 (MQ=255)


                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                    >  2:3298857/1‑101 (MQ=255)


                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCAACGGATGTGTCATGGGCTTTCg                                    >  1:4734142/1‑101 (MQ=255)


                                                                    gCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGc                                >  1:201608/1‑101 (MQ=255)


                                                                     cAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGcc                               <  1:3337922/101‑1 (MQ=255)


                                                                      aGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCt                              <  2:3243329/101‑1 (MQ=255)


                                                                       gCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTc                             <  1:1384144/101‑1 (MQ=255)


                                                                        cGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTcc                            <  1:3253483/101‑1 (MQ=255)


                                                                        cGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTcc                            <  1:2035069/101‑1 (MQ=255)


                                                                        cGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTcc                            >  2:2996127/1‑101 (MQ=255)


                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGGCGGATGTGTCATGGGCTTTCGCAGCCTccc                           >  1:1258341/1‑101 (MQ=255)


                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGGTGTGTCATGGGCTTTCGCAGCCTccc                           >  1:2633956/1‑101 (MQ=255)


                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                           <  1:897432/101‑1 (MQ=255)


                                                                          tGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                          <  1:2764148/101‑1 (MQ=255)


                                                                           ggACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                         <  1:2568864/101‑1 (MQ=255)


                                                                            gacagcgacagcGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGaa                        <  1:1145879/101‑1 (MQ=255)


                                                                            gacagcgacagcGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGaa                        >  1:1329349/1‑101 (MQ=255)


                                                                              cagcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGt                      >  1:2949577/1‑101 (MQ=255)


                                                                              cagcgacagcgGCCTCCCGGC.CCCCCCCCCGTCCAAAGCTCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGt                      <  1:3148834/101‑1 (MQ=255)


                                                                              cagcgacagcgGCCCCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGt                      <  1:3983274/101‑1 (MQ=255)


                                                                               agcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGtt                     >  1:2825265/1‑101 (MQ=255)


                                                                               agcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGtt                     >  1:1268405/1‑101 (MQ=255)


                                                                               agcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGtt                     >  2:1833496/1‑101 (MQ=255)


                                                                                gccacagcgGCCGCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTg                    <  1:1550029/98‑1 (MQ=255)


                                                                                 cgacagcgGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgc                   <  2:607901/101‑1 (MQ=255)


                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                 >  2:1773853/1‑101 (MQ=255)


                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                 >  1:3768990/1‑101 (MQ=255)


                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                 <  2:2688425/101‑1 (MQ=255)


                                                                                   acagcgGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcgc                 <  1:3458336/101‑1 (MQ=255)


                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCaa               >  1:1932141/1‑101 (MQ=255)


                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCaa               >  2:2230025/1‑101 (MQ=255)


                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc             <  2:240041/101‑1 (MQ=255)


                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc             >  1:1125272/1‑101 (MQ=255)


                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc            >  2:3800357/1‑101 (MQ=255)


                                                                                         gCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcg           >  1:3041050/1‑101 (MQ=255)


                                                                                         gCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcg           <  1:3078339/101‑1 (MQ=255)


                                                                                          ccTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgc          >  1:4581330/1‑101 (MQ=255)


                                                                                           cTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGGCTCGTCTGTCGCGGCGGCGGATGTGTCATGGGCTTTCGCAGCCTCCCGTAGTTGCGCAACCcccg         >  2:3186869/1‑101 (MQ=255)


                                                                                            tCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCgg        >  1:3723981/1‑101 (MQ=255)


                                                                                               cGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGGCGCGGCGACGGATGGGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCCCGGccg     >  1:514968/1‑99 (MQ=255)


                                                                                                 ggccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGc    <  2:4462194/98‑1 (MQ=255)


                                                                                                  gccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCa   <  2:1450431/99‑1 (MQ=255)


                                                                                                   ccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAg  <  2:4308415/100‑1 (MQ=255)


GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAG  >  NC_014034/2617678‑2617877

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG