Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,201 Δ1 bp 100% intergenic (‑177/+99) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2010A→.100.0% 104.3 149intergenic (‑177/+99)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (66/58):  ref base (13/12):  total (79/70)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.86e-03

Only 100 of 163 total aligned reads displayed.

GGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640103‑2640297
                                                                                                    |                                                                                                
ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                 <  1:4281870/101‑1 (MQ=25)
ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                 <  1:3058003/101‑1 (MQ=25)
 gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                <  2:4425031/101‑1 (MQ=25)
 gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                <  1:2814169/101‑1 (MQ=25)
   gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                              >  2:607453/1‑99 (MQ=25)
     gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                            <  2:1861871/101‑5 (MQ=21)
     gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                            <  2:3277733/101‑5 (MQ=25)
         aCCGGGGCGCTTTGGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                       <  2:188200/101‑1 (MQ=21)
           cGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCCCCTTGG.CTTCTGCCAATTCCCACCCCCCGGGATTTTGGGCAAGATGAAAAAACAAAggg                                                                                      >  1:567806/1‑101 (MQ=255)
            gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                                    <  2:1884910/101‑1 (MQ=25)
            gTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGtt                                                                                    >  1:2352669/1‑101 (MQ=21)
             tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:4109557/101‑1 (MQ=25)
             tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:1837944/101‑1 (MQ=25)
              ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                  <  1:3999301/101‑1 (MQ=25)
                   ttttGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                             <  2:4416073/101‑1 (MQ=25)
                       gcgcAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:1669204/101‑1 (MQ=25)
                         cgacccgaaaAGGGGGCGCTG.CCCCCCGCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCaaa                                                                      <  1:77379/94‑1 (MQ=17)
                           ccccgaaaAGGGGGCTCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                    <  1:1989155/96‑1 (MQ=17)
                           aGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                     <  1:4694603/101‑1 (MQ=21)
                           aGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                     <  2:2473578/101‑1 (MQ=25)
                            ggCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                    <  2:3246101/101‑1 (MQ=25)
                            ggCAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                    <  2:2394440/101‑1 (MQ=25)
                             ccgaaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                  <  2:2346504/98‑1 (MQ=17)
                              cgaaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATcc                                                                 >  1:3545823/3‑101 (MQ=9)
                              cAAAAGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                                  <  2:3398727/101‑1 (MQ=25)
                               caaaGGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                 <  2:2358587/100‑1 (MQ=21)
                                aaaGGGGGGCGCTC.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGTGGTATTTGGAGCAAGATG.AAAGACTATGGGTTCATCTTGCCTCAAATATccc                                                                <  1:3142659/101‑1 (MQ=18)
                                aaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  2:1113414/1‑101 (MQ=17)
                                aaaAGGGGGCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  1:3545633/101‑1 (MQ=17)
                                 aaagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:490676/4‑101 (MQ=17)
                                 aaagggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:2693804/4‑101 (MQ=17)
                                   ggggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                             >  1:3464504/1‑101 (MQ=21)
                                   agggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                            <  1:2454564/100‑1 (MQ=18)
                                   agggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                            >  1:4582159/2‑101 (MQ=18)
                                    gggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                           >  2:2963111/1‑101 (MQ=18)
                                     ggggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggggg                                                           <  1:4196744/101‑1 (MQ=25)
                                      gggCGCTG.CCCCCCTCTTGGCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                          <  1:3107933/101‑1 (MQ=25)
                                      gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                         <  1:2542500/101‑3 (MQ=18)
                                      gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGCGGGATTTGGAGAAAGATGAAAA.ACCATGGGTTCATCTTTCCTCAAATATCCCgggggg                                                          >  2:4276616/1‑101 (MQ=21)
                                      gggCGCTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCCGGCATTTTGAGCAAGATG.AAAGACCATGGGGTCATCTTTCCTCAAATATCCCcggggg                                                          >  2:1127926/1‑101 (MQ=16)
                                        gcgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                       <  2:2658265/101‑5 (MQ=21)
                                        gcgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                        <  1:3925227/101‑1 (MQ=25)
                                         cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      <  1:1167898/101‑6 (MQ=21)
                                         cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      <  1:2825796/101‑6 (MQ=21)
                                         cgcTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGt                                                       <  1:2353004/101‑1 (MQ=25)
                                          ccTG.CCCGCTTATTGGCCTTCGGCCAAATCTCCCCCCGGGAAATTTGCGTGAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                     <  2:1188943/100‑7 (MQ=21)
                                           cTG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                     <  1:1923838/101‑1 (MQ=25)
                                            tG.CCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    <  1:3350898/101‑1 (MQ=25)
                                                cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                 <  2:3327975/101‑1 (MQ=37)
                                                 ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                <  2:430373/101‑1 (MQ=37)
                                                  cccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                               <  2:2289244/101‑1 (MQ=37)
                                                    ctctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  2:3482552/1‑101 (MQ=37)
                                                     tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                            <  1:1459164/101‑1 (MQ=37)
                                                      ctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                           <  1:1567318/101‑1 (MQ=37)
                                                        tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  1:726826/5‑101 (MQ=37)
                                                        tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  1:4490279/5‑101 (MQ=37)
                                                        tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGACCTGAAAGGACggg                                         <  2:2008335/97‑1 (MQ=25)
                                                         ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  1:2561448/4‑101 (MQ=37)
                                                               cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                  <  2:3464292/101‑1 (MQ=255)
                                                               cGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGAGGGCaga                                  >  2:4177219/1‑101 (MQ=37)
                                                                  ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                               >  2:1781543/1‑101 (MQ=255)
                                                                   cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              <  2:1048494/101‑1 (MQ=255)
                                                                    aaTTCACCCCCCGAGGTATTTGGGGCAAGATG.AAAGACCGTGGGATCATCTTGCCTCAAATATCGCGCCGGGAGTCCTGAAAGGAGGGGGGGCACAGcgcc                             >  2:3818712/1‑101 (MQ=19)
                                                                    aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:2778012/1‑101 (MQ=255)
                                                                    aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:1247573/1‑101 (MQ=255)
                                                                     aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            <  2:1989199/101‑1 (MQ=255)
                                                                     aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            >  1:1362666/1‑101 (MQ=255)
                                                                      ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGTGGCAGAGcccccc                           >  2:2976755/1‑101 (MQ=25)
                                                                        caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         <  2:3146606/99‑1 (MQ=255)
                                                                        caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         >  2:203479/3‑101 (MQ=255)
                                                                         accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        >  1:1145054/2‑101 (MQ=255)
                                                                         accccccGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTGCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        <  1:2169463/100‑1 (MQ=25)
                                                                           ctcccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                      <  2:665110/99‑1 (MQ=255)
                                                                           cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                      <  2:2421040/101‑1 (MQ=255)
                                                                           cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                      >  2:2975898/1‑101 (MQ=255)
                                                                            ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     >  1:4513128/1‑101 (MQ=255)
                                                                            ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     <  2:1454458/101‑1 (MQ=255)
                                                                             cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgag                    >  1:665174/1‑99 (MQ=255)
                                                                               cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTc                  >  1:4041019/1‑101 (MQ=255)
                                                                                  ggTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCttt               <  2:1766876/101‑1 (MQ=255)
                                                                                    tATTTGGAGCAAGATG.AAAGTCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             <  1:380306/101‑1 (MQ=255)
                                                                                    tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  2:2754765/1‑101 (MQ=255)
                                                                                    tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:88993/1‑101 (MQ=255)
                                                                                     aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            >  1:3761020/1‑101 (MQ=255)
                                                                                     aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            >  2:705936/1‑101 (MQ=255)
                                                                                      tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  1:4720246/1‑101 (MQ=255)
                                                                                      tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  2:3686629/101‑1 (MQ=255)
                                                                                       ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          >  2:809106/1‑101 (MQ=255)
                                                                                       ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          <  2:2871811/101‑1 (MQ=255)
                                                                                        tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         >  1:3185223/1‑101 (MQ=255)
                                                                                         ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:1055013/1‑101 (MQ=255)
                                                                                         ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  1:694005/1‑101 (MQ=255)
                                                                                         ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:4621315/1‑101 (MQ=255)
                                                                                         ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        <  1:1041480/101‑1 (MQ=255)
                                                                                           agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt      >  1:1831823/3‑101 (MQ=255)
                                                                                            gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt     <  2:4674532/100‑1 (MQ=255)
                                                                                             cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  2:3819793/1‑101 (MQ=255)
                                                                                             cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  1:1423003/1‑101 (MQ=255)
                                                                                               aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  1:3297876/101‑1 (MQ=255)
                                                                                               aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  1:1029815/1‑101 (MQ=255)
                                                                                                    |                                                                                                
GGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTG.CCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640103‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG