Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,758 Δ1 bp 1.7% intergenic (+101/‑59) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7580G→. 1.7% 6.6 ‑1.8 ‑1.7293intergenic (+101/‑59)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (173/115):  total (174/119)
Fisher's exact test strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.03e-02

Only 100 of 318 total aligned reads displayed.

AGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTT  >  NC_014034/2950658‑2950858
                                                                                                       |                                                                                                      
agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCg                                                                                                        <  2:2557145/101‑1 (MQ=255)
 gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGt                                                                                                       >  2:3011852/1‑101 (MQ=255)
 gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGt                                                                                                       >  1:4527545/1‑101 (MQ=255)
  caagcCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTTGGGCAAAAAGAAAACCCCCCCCTTTATCTTCCCCCCAAAATCCC.GGGG..GGGGATTTGCCGTc                                                                                                      >  1:3383289/1‑101 (MQ=9)
  caagcCGGGGGCTCTGCCCCCGGACCCCCGGGACATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGGCCCAAATATCCC.GGGG..GTGAATTTGCCGTc                                                                                                      >  1:1889350/1‑101 (MQ=255)
   aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCa                                                                                                     >  2:276749/1‑101 (MQ=255)
   aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCa                                                                                                     <  2:2469831/101‑1 (MQ=255)
     gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAgg                                                                                                   <  2:909018/101‑1 (MQ=255)
     gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAgg                                                                                                   <  1:1517517/101‑1 (MQ=255)
       cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCa                                                                                                 <  2:4601102/101‑1 (MQ=255)
        gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCaa                                                                                                >  2:2355644/1‑101 (MQ=255)
        gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCaa                                                                                                >  1:4186376/1‑101 (MQ=255)
        gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GGGAATTTGCCGTCAGGcca                                                                                                >  1:943798/1‑99 (MQ=255)
         ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCaaa                                                                                               >  2:3177569/1‑101 (MQ=255)
          gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAag                                                                                              <  1:2642116/101‑1 (MQ=255)
           ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAaga                                                                                             <  2:1619554/101‑1 (MQ=255)
           ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAaga                                                                                             <  2:2861373/101‑1 (MQ=255)
               ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCGTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.gggg                                                                                        >  2:4102474/1‑101 (MQ=255)
                cttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTGGCCG.AAGGCCAAGA.gggg.gg                                                                                     >  2:2067505/10‑100 (MQ=25)
                 ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTGGCCG.AAGGCCAAGA.gggg.ggc                                                                                    >  1:3681076/9‑99 (MQ=25)
                 gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAAA.gggg.gg                                                                                     >  1:155802/1‑100 (MQ=255)
                  cccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCa                                                                                    <  2:1803456/101‑1 (MQ=255)
                    cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGc                                                                                  >  2:509551/1‑101 (MQ=255)
                     cgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGG..GTGAATTGGCC.AAAGGCCAAGA.GGGG.GCAGcgc                                                                                <  2:3215774/96‑3 (MQ=2)
                      cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTc                                                                                >  2:439562/1‑101 (MQ=255)
                       ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTcc                                                                               >  1:2280564/1‑101 (MQ=255)
                       ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTcc                                                                               >  2:1540921/1‑101 (MQ=255)
                       ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGG..GTGAATTGGCC.AAAGGCCAAGA.GGGG.GCAGCGccc                                                                              >  2:2696761/4‑101 (MQ=2)
                        tccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGG..GTGAATTGGCC.AAAGGCCAAGA.GGGG.GCAGCGcccc                                                                             <  1:1608539/99‑1 (MQ=2)
                        gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTccc                                                                              >  1:3589380/1‑101 (MQ=255)
                         aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTcccc                                                                             >  1:4271625/1‑101 (MQ=255)
                           ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCt                                                                           <  1:1433221/101‑1 (MQ=255)
                             ccGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GGGAATTGGCCG.AAGGCCAAGC.GGGGCGCAGCGCCCCCttt                                                                         >  2:754805/1‑99 (MQ=18)
                               gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGccc                                                                       <  2:125936/101‑1 (MQ=255)
                                ggATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTGGCCG.AAGGCCAAGAGGGGG.GCAGCGCCCCCttttcg                                                                      >  2:3693302/1‑96 (MQ=17)
                                ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGcccc                                                                      >  1:2890378/1‑101 (MQ=255)
                                  atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCgg                                                                    >  2:4378238/1‑101 (MQ=255)
                                  atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGCCGGGCAAAGA.GGGG.GCAGCTTCCCCGGCCCCgg                                                                    >  1:1140624/1‑101 (MQ=25)
                                   tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                   <  2:2771842/101‑1 (MQ=255)
                                   tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                   >  2:3315493/1‑101 (MQ=255)
                                   tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                   <  2:3417831/101‑1 (MQ=255)
                                   tatTTGAGGCAAGATCAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                   <  2:1877120/101‑1 (MQ=255)
                                    atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCt                                                                  >  1:814222/1‑101 (MQ=255)
                                     tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTg                                                                 <  2:4186130/101‑1 (MQ=255)
                                     tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTg                                                                 >  2:4515207/1‑101 (MQ=255)
                                     tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTg                                                                 >  2:13493/1‑101 (MQ=255)
                                      ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                <  2:2188631/101‑1 (MQ=255)
                                      ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                <  2:1595881/101‑1 (MQ=255)
                                        gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                              <  2:2900780/101‑1 (MQ=255)
                                        gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                              >  2:591324/1‑101 (MQ=255)
                                        gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                              >  2:1095417/1‑101 (MQ=255)
                                           gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagggag                                                           >  1:2362729/1‑101 (MQ=255)
                                                aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                                      <  1:142613/101‑1 (MQ=255)
                                                aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                                      >  2:3088732/1‑101 (MQ=255)
                                                aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                                      >  1:4748811/1‑101 (MQ=255)
                                                 tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                     <  1:1808975/101‑1 (MQ=255)
                                                 tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                     >  1:4409298/1‑101 (MQ=255)
                                                  gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                    >  1:3092623/1‑101 (MQ=255)
                                                  gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                    <  2:2382042/101‑1 (MQ=255)
                                                   aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAcg                                                   >  1:2767068/1‑100 (MQ=255)
                                                    aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                                  >  1:3973281/1‑101 (MQ=255)
                                                      cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                                                <  1:1672176/101‑1 (MQ=255)
                                                          ccccTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAcc                                            >  1:170749/1‑101 (MQ=255)
                                                           cccTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                                           <  1:4514302/101‑1 (MQ=255)
                                                             cTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                         >  1:3325181/1‑101 (MQ=255)
                                                              tttCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                        >  1:3866811/1‑101 (MQ=255)
                                                               ttCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                       >  1:3151733/1‑101 (MQ=255)
                                                               ttCATCTTGCCCCAAATAGCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                       <  1:4608082/101‑1 (MQ=255)
                                                                tCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                      <  2:1681447/101‑1 (MQ=255)
                                                                    cTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                  <  1:4698871/101‑1 (MQ=255)
                                                                     ttGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg                                 >  2:1136435/1‑101 (MQ=255)
                                                                      tGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                                >  2:3265105/1‑101 (MQ=255)
                                                                      ctccccAAAAATCCC.GGGGGTGTGAATTCGCCGAGAGAAAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                  <  2:3655313/99‑1 (MQ=255)
                                                                       gCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGcc                               >  1:3467611/1‑101 (MQ=255)
                                                                        ccccAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                              <  1:1001811/101‑1 (MQ=255)
                                                                         cccAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                             <  2:4036686/101‑1 (MQ=255)
                                                                           cAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                           >  1:783209/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                          >  2:4249134/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                          >  1:239635/1‑101 (MQ=255)
                                                                            aaaTATCCA.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                          <  1:4102716/101‑1 (MQ=255)
                                                                              atatCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                        >  2:3955886/1‑101 (MQ=255)
                                                                               tatCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                       >  2:4483415/1‑101 (MQ=255)
                                                                               tatCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                       >  1:2941462/1‑101 (MQ=255)
                                                                                attcc.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                      >  1:2707183/4‑101 (MQ=255)
                                                                                atCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                      <  1:4152447/101‑1 (MQ=255)
                                                                                    c.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc                  >  2:1388538/1‑101 (MQ=255)
                                                                                      gggg..gTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                 >  1:1227201/1‑101 (MQ=255)
                                                                                      gggg..gTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                 <  2:2305601/101‑1 (MQ=255)
                                                                                      gggg..gTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                 >  1:565558/1‑101 (MQ=255)
                                                                                       ggg..gTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc                <  2:3531056/101‑1 (MQ=255)
                                                                                              gAATTTGCCGTCAGGCAAAGC.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg           >  1:374311/1‑101 (MQ=255)
                                                                                              gAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGCCCCGCCTGATCGAAGAGGCCggcgcg           >  1:2135088/1‑101 (MQ=255)
                                                                                              gAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg           <  1:3019338/101‑1 (MQ=255)
                                                                                               aaTTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa          >  1:2492040/1‑101 (MQ=255)
                                                                                                aTTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt         >  2:4626313/1‑101 (MQ=255)
                                                                                                aTTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt         >  1:3800783/1‑101 (MQ=255)
                                                                                                  ttGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg       >  2:4576830/1‑101 (MQ=255)
                                                                                                   tGCCGTAAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCAGATCGAAGAGGCCCCCGCGATCgg      >  1:974680/1‑101 (MQ=255)
                                                                                                      cGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt   <  2:1821104/101‑1 (MQ=255)
                                                                                                       gTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCttt  <  1:3245032/101‑1 (MQ=255)
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AGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTTGCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTT  >  NC_014034/2950658‑2950858

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG