Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,759 Δ1 bp 2.4% intergenic (+102/‑58) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7590T→. 2.4% 10.9 ‑0.4 ‑0.2292intergenic (+102/‑58)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (165/115):  total (171/121)
Fisher's exact test strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.02e-01

Only 100 of 318 total aligned reads displayed.

GCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTG  >  NC_014034/2950659‑2950859
                                                                                                         |                                                                                                      
gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGt                                                                                                        >  2:3011852/1‑101 (MQ=255)
gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGt                                                                                                        >  1:4310411/1‑101 (MQ=255)
  aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCa                                                                                                      >  2:276749/1‑101 (MQ=255)
  aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCa                                                                                                      <  2:2469831/101‑1 (MQ=255)
  aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCa                                                                                                      <  1:827861/101‑1 (MQ=255)
    gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAgg                                                                                                    <  2:909018/101‑1 (MQ=255)
      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCa                                                                                                  <  2:4601102/101‑1 (MQ=255)
      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GGGAATTT.GCCGTCAGGCa                                                                                                  >  1:1745077/1‑101 (MQ=255)
       gggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCaa                                                                                                 >  2:2355644/1‑101 (MQ=255)
        ggggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCaaa                                                                                                >  2:3177569/1‑101 (MQ=255)
         gggCTCTGCCCCCGGTCCCCCCGGATTTTTGGGGGCAAGATGAAACCCGCCCCTTTCATCTTG.CCCAAATTTTCC.GGGG..GTGAATTT.GCCGTCAGGCAAag                                                                                               <  2:1106538/101‑1 (MQ=21)
          ggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAaga                                                                                              <  2:2861373/101‑1 (MQ=255)
          ggCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAaga                                                                                              <  2:1620731/101‑1 (MQ=255)
            ctctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.gg                                                                                           >  1:1952527/1‑101 (MQ=255)
            ctctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.gg                                                                                           <  2:1607431/101‑1 (MQ=255)
              ctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCGTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.gggg                                                                                         >  2:4102474/1‑101 (MQ=255)
              ctGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.gggg                                                                                         >  2:1767295/1‑101 (MQ=255)
               tGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.gggg.g                                                                                       >  2:1804674/1‑101 (MQ=255)
               tGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.gggg.g                                                                                       >  1:1681552/1‑101 (MQ=255)
               cttcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTG.GCCG.AAGGCCAAGA.gggg.gg                                                                                      >  2:2067505/10‑100 (MQ=25)
                ttcgcctcaCCCCCGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTG.GCCG.AAGGCCAAGA.gggg.ggc                                                                                     >  1:4116521/9‑99 (MQ=25)
                 cccccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCa                                                                                     <  1:3803636/101‑1 (MQ=255)
                  ccccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAg                                                                                    <  1:2420912/101‑1 (MQ=255)
                   cccGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGc                                                                                   >  2:509551/1‑101 (MQ=255)
                    cgctccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGG..GTGAATTG.GCC.AAAGGCCAAGA.GGGG.GCAGcgc                                                                                 <  2:3215774/96‑3 (MQ=2)
                     cGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTc                                                                                 >  2:439562/1‑101 (MQ=255)
                      ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTcc                                                                                <  2:1546001/101‑1 (MQ=255)
                      ggACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTcc                                                                                >  1:2363703/1‑101 (MQ=255)
                      ctccccccGGGATATTT.GCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGG..GTGAATTG.GCC.AAAGGCCAAGA.GGGG.GCAGCGccc                                                                               >  2:2696761/4‑101 (MQ=2)
                        aCCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTcccc                                                                              >  1:2688823/1‑101 (MQ=255)
                          ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCt                                                                            <  1:945291/101‑1 (MQ=255)
                          ccccGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCt                                                                            <  1:2959554/101‑1 (MQ=255)
                            ccGGGATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GGGAATTG.GCCG.AAGGCCAAGC.GGGGCGCAGCGCCCCCttt                                                                          >  2:754805/1‑99 (MQ=18)
                               ggATATTT.GGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGG..GTGAATTG.GCCG.AAGGCCAAGAGGGGG.GCAGCGCCCCCttttcg                                                                       >  2:3693302/1‑96 (MQ=17)
                                gATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCg                                                                      >  1:491/1‑101 (MQ=255)
                                 atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCgg                                                                     >  2:4378238/1‑101 (MQ=255)
                                 atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCgg                                                                     >  2:2635041/1‑101 (MQ=255)
                                 atatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGCCGGGCAAAGA.GGGG.GCAGCTTCCCCGGCCCCgg                                                                     >  1:1140624/1‑101 (MQ=25)
                                  tatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                    <  2:2771842/101‑1 (MQ=255)
                                  tatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                    >  2:3315493/1‑101 (MQ=255)
                                  tatTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGc                                                                    <  2:3417831/101‑1 (MQ=255)
                                   atTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCt                                                                   >  1:56973/1‑101 (MQ=255)
                                    ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTg                                                                  >  2:4515207/1‑101 (MQ=255)
                                    ttt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTg                                                                  <  2:4186130/101‑1 (MQ=255)
                                     tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                 >  1:4616179/1‑101 (MQ=255)
                                     tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                 <  1:3870864/101‑1 (MQ=255)
                                     tt.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTga                                                                 <  2:2188631/101‑1 (MQ=255)
                                        gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                               <  2:2900780/101‑1 (MQ=255)
                                        gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagg                                                               >  2:591324/1‑101 (MQ=255)
                                          ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgaggga                                                             <  1:4524893/101‑1 (MQ=255)
                                          ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgaggga                                                             <  1:3390470/101‑1 (MQ=255)
                                           gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTgagggag                                                            <  1:2727203/101‑1 (MQ=255)
                                              aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGTCg                                                         >  1:3727944/1‑101 (MQ=255)
                                                aTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa                                                       >  2:3088732/1‑101 (MQ=255)
                                                 tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                      >  1:2305734/1‑101 (MQ=255)
                                                  gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                     <  2:2382042/101‑1 (MQ=255)
                                                  gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                     >  1:3655542/1‑101 (MQ=255)
                                                    aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                                   >  1:4214212/1‑101 (MQ=255)
                                                        cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                               <  1:276783/101‑1 (MQ=255)
                                                        cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                               >  1:2550916/1‑101 (MQ=255)
                                                            ccTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCtt                                           >  1:4463017/1‑101 (MQ=255)
                                                              tttCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                         <  1:1260357/101‑1 (MQ=255)
                                                               ttCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                        <  1:417158/101‑1 (MQ=255)
                                                                 cATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                      >  2:1850804/1‑101 (MQ=255)
                                                                 cATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                      >  1:787846/1‑101 (MQ=255)
                                                                      tGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                                 >  2:3265105/1‑101 (MQ=255)
                                                                      ctccccAAAAATCCC.GGGGGTGTGAATTC.GCCGAGAGAAAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                   <  2:3655313/99‑1 (MQ=255)
                                                                        ccccAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCt                               <  1:1001811/101‑1 (MQ=255)
                                                                         cccAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTg                              <  2:4036686/101‑1 (MQ=255)
                                                                            aaaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                           >  1:1518001/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                           >  1:1502308/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                           >  2:4249134/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                           >  1:3180795/1‑101 (MQ=255)
                                                                             aaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccagatcggaag                          >  1:1612850/1‑91 (MQ=255)
                                                                             aaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                          >  2:1349904/1‑101 (MQ=255)
                                                                             aaTATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                          >  1:2146819/1‑101 (MQ=255)
                                                                              atatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                         >  2:3955886/1‑101 (MQ=255)
                                                                              atatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGa                         <  1:2896590/101‑1 (MQ=255)
                                                                               tatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.TGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                        <  1:4065913/101‑1 (MQ=255)
                                                                               tatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                        >  2:4483415/1‑101 (MQ=255)
                                                                               tatCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGaa                        >  2:1045339/1‑101 (MQ=255)
                                                                                  cgcggggg..gGGAATTTGGCCGAGAGAAAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAag                       <  1:3118596/97‑1 (MQ=255)
                                                                                    c.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc                   >  1:3269617/1‑101 (MQ=255)
                                                                                    c.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGc                   >  2:1456889/1‑101 (MQ=255)
                                                                                      gggg..gTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                  <  2:2305601/101‑1 (MQ=255)
                                                                                       ggg..gTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGccc                 <  2:3531056/101‑1 (MQ=255)
                                                                                              gAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg            >  1:3468258/1‑101 (MQ=255)
                                                                                              gAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg            >  1:3341728/1‑101 (MQ=255)
                                                                                              gAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg            >  1:1429856/1‑101 (MQ=255)
                                                                                                aTTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt          >  2:4626313/1‑101 (MQ=255)
                                                                                                 ttt.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc         >  1:3771084/1‑101 (MQ=255)
                                                                                                 ttt.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc         >  1:4739829/1‑101 (MQ=255)
                                                                                                 ttt.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc         >  2:1294164/1‑101 (MQ=255)
                                                                                                  tt.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg        >  2:4576830/1‑101 (MQ=255)
                                                                                                      ccGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCt     >  1:4568585/1‑101 (MQ=255)
                                                                                                       cGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt    <  1:3048833/101‑1 (MQ=255)
                                                                                                       cGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt    <  1:1864444/101‑1 (MQ=255)
                                                                                                       cGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt    >  2:1161775/1‑101 (MQ=255)
                                                                                                        gTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGcaga   >  1:1559222/1‑98 (MQ=255)
                                                                                                         tCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg  <  2:1894481/101‑1 (MQ=255)
                                                                                                         |                                                                                                      
GCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTT.GAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGG..GTGAATTT.GCCGTCAGGCAAAGA.GGGG.GCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTG  >  NC_014034/2950659‑2950859

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG