Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,003,990 Δ1 bp 1.9% intergenic (+36/+140) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,003,9900G→. 1.9% 3.9 ‑0.5 ‑0.7212intergenic (+36/+140)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (77/128):  total (81/131)
Fisher's exact test strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.17e-01

Only 100 of 231 total aligned reads displayed.

GGACGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA  >  NC_014034/3003890‑3004090
                                                                                                    |                                                                                                       
ggACGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTg                                                                                                         <  2:2496824/101‑1 (MQ=255)
ggACGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTg                                                                                                         <  2:4653212/101‑1 (MQ=255)
ggACGCTGGAAGAGACGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCCAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTg                                                                                                         <  1:4720628/101‑1 (MQ=255)
  aCGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.c                                                                                                      >  2:3054676/1‑101 (MQ=255)
   cGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.Cg                                                                                                     >  1:493079/1‑101 (MQ=255)
   cGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.Cg                                                                                                     <  2:3070677/101‑1 (MQ=255)
      tGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGcc                                                                                                  <  1:2234194/101‑1 (MQ=255)
          agagGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAAtt                                                                                              <  1:3952835/101‑1 (MQ=255)
          agagGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAAtt                                                                                              >  1:2730090/1‑101 (MQ=255)
           gagGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTc                                                                                             >  2:3180109/1‑101 (MQ=255)
               cgcgcAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.ccc                                                                                        >  2:2290774/1‑101 (MQ=255)
                  gcAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.cccccc                                                                                     <  1:1434228/101‑1 (MQ=255)
                   cAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.g                                                                                   <  1:1812294/101‑1 (MQ=255)
                   cAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.g                                                                                   <  1:1573149/101‑1 (MQ=255)
                    aaGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.gg                                                                                  <  2:1068889/101‑1 (MQ=255)
                    aaGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.gg                                                                                  <  2:1289322/101‑1 (MQ=255)
                    aaGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.gg                                                                                  >  1:4342164/1‑101 (MQ=255)
                     aGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.ggg                                                                                 >  1:3061610/1‑101 (MQ=255)
                     aGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.ggg                                                                                 >  2:1185628/1‑101 (MQ=255)
                      gggACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGa                                                                                <  1:943337/101‑1 (MQ=255)
                       ggACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGat                                                                               <  1:3009787/101‑1 (MQ=255)
                       ggACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGCCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGat                                                                               <  2:902560/101‑1 (MQ=255)
                        gACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGata                                                                              <  2:636812/101‑1 (MQ=255)
                          cAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATAtt                                                                            >  2:2369630/1‑101 (MQ=255)
                           aGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGCTAttt                                                                           >  2:267033/1‑101 (MQ=255)
                           aGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATAttt                                                                           <  1:4190896/101‑1 (MQ=255)
                                ttgatCGCTCTGGTGCGCGCGTGCGAGATCTGAGGGCGAGGCGTGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGAcc                                                                      <  2:1134231/100‑1 (MQ=37)
                                atgatCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGAcc                                                                      <  1:369009/101‑1 (MQ=255)
                                atgatCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGAcc                                                                      <  2:2463909/101‑1 (MQ=255)
                                  gatcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCaa                                                                    >  2:3421942/1‑101 (MQ=255)
                                  gatcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCaa                                                                    <  1:105957/101‑1 (MQ=255)
                                   atcgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAg                                                                   <  2:967232/101‑1 (MQ=255)
                                     cgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAggg                                                                 >  2:1012592/1‑100 (MQ=255)
                                     cgatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.CGGATATTTGGACCAAggg                                                                 >  1:3364321/1‑100 (MQ=255)
                                      gatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTg                                                                <  2:270055/101‑1 (MQ=255)
                                      gatcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTg                                                                <  1:1161769/101‑1 (MQ=255)
                                      gatcTACTGCGCGCGGGCGAGATCTGAGGGCGAGGCGGGGGGCGCTGCCCCACTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTg                                                                <  1:4652732/101‑1 (MQ=37)
                                        tcTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGaa                                                              <  1:419244/101‑1 (MQ=255)
                                         cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGaaa                                                             >  1:3122161/1‑101 (MQ=255)
                                         cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGaaa                                                             <  2:2758834/101‑1 (MQ=255)
                                         cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGaaa                                                             <  1:213542/101‑1 (MQ=255)
                                         cTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGaaa                                                             <  2:600382/101‑1 (MQ=255)
                                          tGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAg                                                            <  2:3667476/101‑1 (MQ=255)
                                          tGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAg                                                            <  2:794202/101‑1 (MQ=255)
                                          tGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAg                                                            <  1:753655/101‑1 (MQ=255)
                                              gcgcgcGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGa                                                        <  1:1520454/101‑1 (MQ=255)
                                                    gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCgg                                                  <  1:1039795/101‑1 (MQ=255)
                                                      gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGtt                                                >  2:247885/1‑101 (MQ=255)
                                                       cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCGATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                               <  2:2604227/101‑1 (MQ=255)
                                                       cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGttt                                               <  2:3746174/101‑1 (MQ=255)
                                                        gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTg                                              <  1:1260126/101‑1 (MQ=255)
                                                        gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTg                                              <  1:2147208/101‑1 (MQ=255)
                                                        gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTg                                              <  1:2415615/101‑1 (MQ=255)
                                                        aagaGCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTg                                              <  1:4476455/100‑1 (MQ=255)
                                                          gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGGGAAAGCAGATCCCGGTTTGcc                                            >  1:68448/1‑101 (MQ=255)
                                                              tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                        <  1:1719218/101‑1 (MQ=255)
                                                              tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                        <  2:470456/101‑1 (MQ=255)
                                                              tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                        <  2:3065736/101‑1 (MQ=255)
                                                              tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTc                                        <  2:2091994/101‑1 (MQ=255)
                                                               gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCa                                       <  1:19770/101‑1 (MQ=255)
                                                               gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCa                                       <  2:2961727/101‑1 (MQ=255)
                                                                aGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCCGACCGGCCAATTAC.CCCCCC.CCGGCATTTTGTCCAAGGCGAACGCAAACTCCCGCTTGCCTTTcc                                       >  1:3255272/1‑100 (MQ=255)
                                                                 gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCAcc                                     >  2:2895738/1‑101 (MQ=255)
                                                                  ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCt                                    <  2:3681507/101‑1 (MQ=255)
                                                                  ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCt                                    <  1:2959037/101‑1 (MQ=255)
                                                                  ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCt                                    <  1:3986657/101‑1 (MQ=255)
                                                                   gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCAGCtt                                   <  1:4235841/101‑1 (MQ=255)
                                                                   gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCtt                                   >  1:4458149/1‑101 (MQ=255)
                                                                     aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTgg                                 >  2:2781154/1‑101 (MQ=255)
                                                                     aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GCGGTATTTGGCCCCAGGGGAAAGCAGATCCCGGTTTGCCTTTCACCTTgg                                 >  1:2761781/1‑101 (MQ=37)
                                                                      aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgc                                <  1:3813485/101‑1 (MQ=255)
                                                                      aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGAAGATCCCGGTTTGCCTTTCACCTTGgc                                >  1:1679993/1‑101 (MQ=255)
                                                                       tgCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGgcg                               <  1:4295842/100‑1 (MQ=255)
                                                                         cGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCa                             >  1:643338/1‑101 (MQ=255)
                                                                            ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                          <  1:4463576/101‑1 (MQ=255)
                                                                            ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                          >  1:2679359/1‑101 (MQ=255)
                                                                            ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                          <  2:146833/101‑1 (MQ=255)
                                                                            ggggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAat                          <  1:3623501/101‑1 (MQ=255)
                                                                             gggCGCTGCCCCCCTCTTGGCCCGA.CGGCCAATTCACCCCCCC.GGGAAATTTGGCCCCAGGGAAAAATAAAACCGGGTTGTCCTTTTCCCTTTGCCCaaaa                          >  1:4038576/1‑100 (MQ=12)
                                                                              ggCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                        <  1:2606611/101‑1 (MQ=255)
                                                                               gcgcTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                       <  2:2061610/101‑1 (MQ=255)
                                                                                   tGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                   >  1:2318857/1‑101 (MQ=255)
                                                                                    gCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.CGGATATTTGGCCCAAGGGGAAAGCAGCTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                  >  2:1906909/1‑101 (MQ=37)
                                                                                     ccccccTCTTGGCCT.T.CGGCCAATTCA.CCCCCC.GGGATATTTCGGGCAAGAT.AAAGAAGGTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc           <  1:2835562/101‑6 (MQ=37)
                                                                                     ccccccTCTTGGCCT.T.CGGCCAATTCA.CCCCCC.GGGATATTTCGGGCAAGAT.AAAGAAGGTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc           >  2:3977735/1‑96 (MQ=37)
                                                                                     ccccccTCTTGGCCT.T.CGGCCAATTCA.CCCCCC.GGGATATTTCGGGCAAGAT.AAAGAAGGTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc           >  2:1846795/1‑96 (MQ=37)
                                                                                      cccccTCTTGGCCTGA.CGGCCAATTCC.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggggg                <  2:3644218/101‑1 (MQ=255)
                                                                                      cccccTCTTGGCCTGA.CGGCCAATTCA.CCCCCCGGGGAATTTTGGCCCAGGGGGAAAAAAAACCCCGGTTGCCCTTTTCCCTTGCGCCAAAAATCCGgggg                 >  1:3214124/1‑101 (MQ=9)
                                                                                      cccccTCTTGGCCT.T.CGGCCAATTCC.CCCCCC.GGGATATTTCGGGCAAGAT.AAAGAAGGTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagcccg          <  1:287408/101‑7 (MQ=37)
                                                                                          ctctTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGCCGCAAATATCCCGGGGGGAGt            <  1:3494056/101‑1 (MQ=255)
                                                                                           tctTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc           <  2:4516520/101‑1 (MQ=255)
                                                                                           tctTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc           >  1:563196/1‑101 (MQ=255)
                                                                                            ctTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc          >  1:3194945/1‑101 (MQ=255)
                                                                                             ttGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc         >  2:476430/1‑101 (MQ=255)
                                                                                              tGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg        <  2:4348233/101‑1 (MQ=255)
                                                                                              tGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg        >  2:1610889/1‑101 (MQ=255)
                                                                                               ggCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc       >  2:3313535/1‑101 (MQ=255)
                                                                                                   tGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg   <  2:4207429/101‑1 (MQ=255)
                                                                                                    gA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa  <  2:2402087/101‑1 (MQ=255)
                                                                                                    gA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa  >  2:1197034/1‑101 (MQ=255)
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GGACGCTGGAAGAGGCGCGCAAGGGACAGGCGATGATCGATCTGGTGCGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGA.CGGCCAATTCA.CCCCCC.GGGATATTTGGACCAAGGTGAAAGCAGATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGA  >  NC_014034/3003890‑3004090

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG