BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,004,040	Δ1 bp	1.8%	intergenic (+86/+90)	gfo → / ← lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,004,040	0	C→.	1.8%	4.0 0.0 ‑0.1	228	intergenic (+86/+90)	gfo/lrp	glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand): new base (2/2): ref base (116/107): total (118/110)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.91e-01

Only 100 of 256 total aligned reads displayed.


GCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTT  >  NC_014034/3003940‑3004140


  gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACGAAGGTGAAAGCAG.ATCCCgg                                                                                                     <  1:3961264/101‑1 (MQ=255)


  gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCgg                                                                                                     >  2:4653509/1‑101 (MQ=255)


   ggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTAA.CCCCCCGGGATATTTGGACCAAGGAGAAAGCAG.ATCCCGGt                                                                                                    >  1:3789200/1‑101 (MQ=255)


    gCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGtt                                                                                                   >  2:247885/1‑101 (MQ=255)


     ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                  <  1:3420506/100‑1 (MQ=255)


      gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CTCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                 >  2:312810/1‑101 (MQ=255)


      gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                 <  1:1260126/101‑1 (MQ=255)


      aagaGCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                 <  1:4476455/100‑1 (MQ=255)


        gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                               <  1:1465239/101‑1 (MQ=255)


        gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                               >  1:3199351/1‑101 (MQ=255)


        gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                               >  1:3175755/1‑101 (MQ=255)


        gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGcc                                                                                               >  1:68448/1‑101 (MQ=255)


         aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                              <  1:2987196/101‑1 (MQ=255)


         aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                              <  2:3646444/101‑1 (MQ=255)


          tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACTGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                             <  2:3943887/101‑1 (MQ=255)


          tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                             <  2:4404698/101‑1 (MQ=255)


            tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                           <  2:2091994/101‑1 (MQ=255)


            tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                           <  2:2119793/101‑1 (MQ=255)


            tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                           <  2:470456/101‑1 (MQ=255)


             gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                          <  2:1100032/101‑1 (MQ=255)


             gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                          <  1:19770/101‑1 (MQ=255)


             gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGAAG.ATCCCGGTTTGCCTTTCa                                                                                          >  2:3567171/1‑101 (MQ=255)


               gggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAcc                                                                                        >  2:2895738/1‑101 (MQ=255)


                 gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCAGCtt                                                                                      <  1:4235841/101‑1 (MQ=255)


                 gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                      >  1:4458149/1‑101 (MQ=255)


                  cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTg                                                                                     <  1:2273407/101‑1 (MQ=255)


                   aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                    >  2:2781154/1‑101 (MQ=255)


                   aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCCCACGGCCAATTCA.CCCCCCCGCAAATTTTGGCCAAGGGGGGAGCAG.ACCCCGGTTTGCCTTTCCCCTTgg                                                                                    >  1:2415385/1‑101 (MQ=21)


                      gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGgcgc                                                                                 <  1:2143489/101‑1 (MQ=255)


                         gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                              <  1:4511483/101‑1 (MQ=255)


                          ggggggtccccccccTCCTGTCCTGACGGCCAATTCC.CCCCCCGGGATATTTCGCCCAAGGCAAAACCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  2:4314694/92‑1 (MQ=25)


                          ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCC.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  1:4684495/101‑1 (MQ=255)


                          ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  2:146833/101‑1 (MQ=255)


                          ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  1:3623501/101‑1 (MQ=255)


                          ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                             <  2:3057652/101‑1 (MQ=255)


                           gggCGCTGCCCCCCTCTTGGCCCGACGGCCAATTCACCCCCCCGGGAAATTTGGCCCCAGGGAAAAATAA.AACCGGGTTGTCCTTTTCCCTTTGCCCaaaa                                                                             >  1:4038576/1‑100 (MQ=12)


                            ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                           <  1:2606611/101‑1 (MQ=255)


                            ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                           <  1:219207/101‑1 (MQ=255)


                             gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATc                                                                          <  2:2061610/101‑1 (MQ=255)


                                cTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                                       <  1:573122/101‑1 (MQ=255)


                                  gCCCCCCTCTTGGCCTGACGGGCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                     >  1:2697277/1‑101 (MQ=255)


                                  gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                     >  2:1128896/1‑101 (MQ=255)


                                   ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                              <  1:2835562/101‑6 (MQ=37)


                                         tctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                              >  2:2621312/1‑101 (MQ=255)


                                           ttGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTccc                                                            >  2:476430/1‑101 (MQ=255)


                                            tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                           >  1:2874867/1‑101 (MQ=255)


                                             ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                          >  2:3313535/1‑101 (MQ=255)


                                             ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                          >  2:920737/1‑101 (MQ=255)


                                               ccTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                                        >  2:1877623/1‑101 (MQ=255)


                                                  gACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                     <  2:2402087/101‑1 (MQ=255)


                                                     ggCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.gg                                                 >  2:2359500/1‑101 (MQ=255)


                                                        cAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.ggggg                                              <  2:2679205/101‑1 (MQ=255)


                                                         aaTTCA.CCCCCCGGGATATTTGGTCGAAGGAGAAAGCAG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.gggggg                                             >  2:1528381/1‑101 (MQ=25)


                                                         aaTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAG.gggggg                                             >  1:3688875/1‑101 (MQ=255)


                                                          aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGc                                            >  2:2733010/1‑101 (MQ=255)


                                                           ttCC.CCCCCCGGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCa                                           <  1:1012671/101‑1 (MQ=21)


                                                           ttCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCa                                           >  1:2479087/1‑101 (MQ=255)


                                                             cccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGGGTCCCGCAGGGAC.GGGGGGCag                                          >  2:3745347/4‑101 (MQ=18)


                                                             cA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCaga                                         >  1:1480378/1‑101 (MQ=255)


                                                              ccccccccGGGATATTTGGCCCAAGGTGAAATCCC.CCCCCCGTGTTCATTTCCCCTTGGCGGAAATATCCCGGTGGGAGTCCCGCAGGGAC.GGGGGGCagc                                         <  1:460664/99‑2 (MQ=17)


                                                              ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCagc                                         <  2:1966374/99‑2 (MQ=18)


                                                               cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCagag                                        <  1:267058/100‑1 (MQ=255)


                                                                 cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgcc                                      >  1:102579/1‑101 (MQ=18)


                                                                 cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgcc                                      >  1:2758194/1‑101 (MQ=18)


                                                                   cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccc                                    <  1:1638962/101‑1 (MQ=255)


                                                                     cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgcccccc                                  >  2:4382420/1‑101 (MQ=18)


                                                                      gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCTCGGGGGGAGTCCCGCGGGGGC.GGGGGGCAGAgcccccct                                 <  1:502862/101‑1 (MQ=255)


                                                                      gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccct                                 <  2:744798/101‑1 (MQ=255)


                                                                      gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccct                                 >  2:4440822/1‑101 (MQ=255)


                                                                        gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgccccccctc                               >  2:2498202/1‑98 (MQ=18)


                                                                        gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgc                               >  2:2769313/1‑101 (MQ=255)


                                                                        gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGAGCAGAgcccacctgc                               >  1:4614400/1‑101 (MQ=255)


                                                                         atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgccccccctct                              >  2:2282405/1‑97 (MQ=18)


                                                                         atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgccccccctct                              <  2:1196117/101‑5 (MQ=17)


                                                                         atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgcc                              >  2:3501376/1‑101 (MQ=255)


                                                                          tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgccccccctctt                             >  1:2452594/1‑96 (MQ=18)


                                                                          tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGGCAGCgccccccctctt                             <  2:3997574/101‑6 (MQ=17)


                                                                           atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGGCGGCgccccccctctt                             >  2:1675361/1‑96 (MQ=21)


                                                                            tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgccccc                           <  1:3231582/101‑1 (MQ=255)


                                                                             ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgcccccc                          <  1:75970/101‑1 (MQ=255)


                                                                             ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgcccccc                          >  1:4351023/1‑101 (MQ=255)


                                                                             ttGGACCAAGGTGAAAGCAG.AGCCCGGTGTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgcccccc                          <  1:1151881/101‑1 (MQ=255)


                                                                              tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgcccccct                         <  1:2918410/101‑1 (MQ=255)


                                                                              tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAgcccccctgcccccct                         >  2:2957064/1‑101 (MQ=255)


                                                                               ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGcccccctgcccccctg                        >  1:385777/1‑101 (MQ=255)


                                                                                gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTgg                       >  1:548285/1‑101 (MQ=255)


                                                                                   cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCGCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                    <  2:4131107/101‑1 (MQ=255)


                                                                                   cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                    >  1:3487931/1‑101 (MQ=255)


                                                                                   cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                    >  2:1826634/1‑101 (MQ=255)


                                                                                   cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCCGCCCCCCTGGGGc                    >  1:1121768/1‑101 (MQ=255)


                                                                                     aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                  <  2:3788965/101‑1 (MQ=255)


                                                                                     aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                  >  1:1311826/1‑101 (MQ=255)


                                                                                      ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                 >  1:3142866/1‑101 (MQ=255)


                                                                                         gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg              >  1:1731962/1‑101 (MQ=255)


                                                                                           aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt            >  1:4092223/1‑101 (MQ=255)


                                                                                            aGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc           <  2:3194744/101‑1 (MQ=255)


                                                                                             gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa          <  2:120396/101‑1 (MQ=255)


                                                                                               aG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg        >  2:4552435/1‑101 (MQ=37)


                                                                                                    cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg    >  2:776449/1‑101 (MQ=255)


                                                                                                     ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt   >  1:4164827/1‑101 (MQ=255)


GCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAC.GGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTT  >  NC_014034/3003940‑3004140

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG