Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,042 Δ1 bp 1.7% intergenic (+88/+88) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0420G→. 1.7% 4.2 0.0 ‑0.1230intergenic (+88/+88)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (119/106):  total (121/109)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.53e-01

Only 100 of 258 total aligned reads displayed.

GGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTG  >  NC_014034/3003942‑3004142
                                                                                                      |                                                                                                    
gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACGAAGGTGAAAGCAG.ATCCCgg                                                                                                      <  1:3961264/101‑1 (MQ=255)
 ggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTAA.CCCCCCGGGATATTTGGACCAAGGAGAAAGCAG.ATCCCGGt                                                                                                     >  1:3789200/1‑101 (MQ=255)
   ggagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                   <  1:3420506/100‑1 (MQ=255)
    gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                  <  1:1260126/101‑1 (MQ=255)
    aagaGCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                                  <  1:4476455/100‑1 (MQ=255)
      gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                >  1:3199351/1‑101 (MQ=255)
      gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGcc                                                                                                >  1:3175755/1‑101 (MQ=255)
      gaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGGGAAAGCAG.ATCCCGGTTTGcc                                                                                                >  1:68448/1‑101 (MQ=255)
       aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                               <  1:2987196/101‑1 (MQ=255)
        tCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACTGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCtt                                                                                              <  2:3943887/101‑1 (MQ=255)
          tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                            <  1:1719218/101‑1 (MQ=255)
          tGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTc                                                                                            <  2:2119793/101‑1 (MQ=255)
           gAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCa                                                                                           <  1:19770/101‑1 (MQ=255)
              ggCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCt                                                                                        <  2:3681507/101‑1 (MQ=255)
               gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCtt                                                                                       <  2:4733040/101‑1 (MQ=255)
                 aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                     >  2:2781154/1‑101 (MQ=255)
                 aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCCCACGGCCAATTCA.CCCCCCCGCAAATTTTGGCCAAGGGGGGAGCAG.ACCCCGGTTTGCCTTTCCCCTTgg                                                                                     >  1:2415385/1‑101 (MQ=21)
                       gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                               <  1:4511483/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                              <  1:4463576/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                              <  2:3057652/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                              <  1:3623501/101‑1 (MQ=255)
                        ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                              <  2:146833/101‑1 (MQ=255)
                         gggCGCTGCCCCCCTCTTGGCCCGACGGCCAATTCACCCCCCCGGGAAATTTGGCCCCAGGGAAAAATAA.AACCGGGTTGTCCTTTTCCCTTTGCCCaaaa                                                                              >  1:4038576/1‑100 (MQ=12)
                          ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                            <  1:219207/101‑1 (MQ=255)
                          ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                            <  1:2606611/101‑1 (MQ=255)
                            cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                          >  1:2210682/1‑101 (MQ=255)
                             gcTGCCCCCCTCTTGGCNTGACGGCCAATTCA.CCCCCCGGGATATTTGGATCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                         <  2:105216/101‑1 (MQ=255)
                              cTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCg                                                                        <  1:573122/101‑1 (MQ=255)
                               tGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgg                                                                       >  2:2078309/1‑101 (MQ=255)
                                gCCCCCCTCTTGGCCTGACGGGCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                      >  1:2697277/1‑101 (MQ=255)
                                gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                      >  2:1128896/1‑101 (MQ=255)
                                gCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                      >  1:4295272/1‑101 (MQ=255)
                                 ccccccTCTTGGCCT.TCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCTTTCATCTTGCCCGAAATATCCCgggggagccc                                                               <  1:2835562/101‑6 (MQ=37)
                                       tctTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTc                                                               >  2:2621312/1‑101 (MQ=255)
                                          tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                            >  2:1610889/1‑101 (MQ=255)
                                          tGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcg                                                            >  1:2874867/1‑101 (MQ=255)
                                           ggCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                           >  2:920737/1‑101 (MQ=255)
                                               tGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                       <  2:4207429/101‑1 (MQ=255)
                                                gACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGa                                                      <  2:2402087/101‑1 (MQ=255)
                                                  cGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                    <  1:312844/101‑1 (MQ=255)
                                                   ggCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                   >  2:2359500/1‑101 (MQ=255)
                                                   ggCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgg                                                   >  1:1468305/1‑101 (MQ=255)
                                                     ccAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                 >  2:4507949/1‑101 (MQ=255)
                                                      cAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                <  2:2679205/101‑1 (MQ=255)
                                                       aaTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGgggggg                                               >  1:3688875/1‑101 (MQ=255)
                                                       aaTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                               >  1:2087249/1‑101 (MQ=255)
                                                        aTTCG.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.GTCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                              <  2:4409774/101‑1 (MQ=255)
                                                        aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                              <  2:4392376/101‑1 (MQ=255)
                                                        aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                              >  2:2733010/1‑101 (MQ=255)
                                                        aTTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCCGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGGCCCGCGGGGGCggggggg                                              >  2:4024223/1‑100 (MQ=21)
                                                         ttCC.CCCCCCGGGATATTTGGACAAGGGTGAAAGCAG.ATCCCGGTTTGCTTTTCCCCTTGGCGAAAATACCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                             <  1:1012671/101‑1 (MQ=21)
                                                         ttCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                             >  1:2479087/1‑101 (MQ=255)
                                                           cccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGGGTCCCGCAGGGACGGGGGGCag                                            >  2:3745347/4‑101 (MQ=18)
                                                           cA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                           >  1:2635727/1‑101 (MQ=255)
                                                            ccccccccGGGATATTTGGCCCAAGGTGAAATCCC.CCCCCCGTGTTCATTTCCCCTTGGCGGAAATATCCCGGTGGGAGTCCCGCAGGGACGGGGGGCagc                                           <  1:460664/99‑2 (MQ=17)
                                                            ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                           <  2:2893325/99‑2 (MQ=18)
                                                            ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                           <  2:1966374/99‑2 (MQ=18)
                                                               cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                        >  1:2758194/1‑101 (MQ=18)
                                                               cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                        >  1:102579/1‑101 (MQ=18)
                                                               cccccGGGATATTTGGCCCAAGGCGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                        >  2:4338588/1‑101 (MQ=18)
                                                                 cccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccc                                      <  1:1638962/101‑1 (MQ=255)
                                                                   cGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccc                                    <  2:3175555/101‑1 (MQ=255)
                                                                    gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCTCGGGGGGAGTCCCGCGGGGGCGGGGGGCAGAgcccccct                                   <  1:502862/101‑1 (MQ=255)
                                                                    gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                   <  2:744798/101‑1 (MQ=255)
                                                                     ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                  <  2:3858571/101‑3 (MQ=17)
                                                                      gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctc                                 >  2:2498202/1‑98 (MQ=18)
                                                                      gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                 >  2:2769313/1‑101 (MQ=255)
                                                                      gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAgcccacctgc                                 >  1:4614400/1‑101 (MQ=255)
                                                                       atatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                >  2:2282405/1‑97 (MQ=18)
                                                                       atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                >  1:474038/1‑101 (MQ=255)
                                                                        tttttGGCCAAAGGTGAAAGCAG.ATCCCGTTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccc                               <  2:3271633/99‑1 (MQ=255)
                                                                        tatTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                               >  1:2452594/1‑96 (MQ=18)
                                                                        tatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctctt                               <  2:1680358/101‑6 (MQ=17)
                                                                         atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCCGGGGCAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                              >  2:1886475/1‑101 (MQ=255)
                                                                          tttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgccccc                             <  1:3231582/101‑1 (MQ=255)
                                                                           ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                            <  1:75970/101‑1 (MQ=255)
                                                                           ttGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                            >  1:4351023/1‑101 (MQ=255)
                                                                           ttGGACCAAGGTGAAAGCAG.AGCCCGGTGTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                            <  1:1151881/101‑1 (MQ=255)
                                                                            tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                           >  2:2957064/1‑101 (MQ=255)
                                                                            tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                           <  1:2918410/101‑1 (MQ=255)
                                                                             ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                          >  1:385777/1‑101 (MQ=255)
                                                                                ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                       >  2:24176/1‑101 (MQ=255)
                                                                                 cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                      >  1:555074/1‑101 (MQ=255)
                                                                                 cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                      >  2:1826634/1‑101 (MQ=255)
                                                                                 cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                      >  1:3487931/1‑101 (MQ=255)
                                                                                 cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCCGCCCCCCTGGGGc                      >  1:1121768/1‑101 (MQ=255)
                                                                                   aGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                    >  1:1311826/1‑101 (MQ=255)
                                                                                        aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc               >  2:4670203/1‑101 (MQ=255)
                                                                                         aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt              >  1:4092223/1‑101 (MQ=255)
                                                                                           gCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa            <  2:120396/101‑1 (MQ=255)
                                                                                             aG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAgg          >  2:1147549/1‑101 (MQ=255)
                                                                                             aG.ATCCCGGATTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGAGCAGAGCCCCCCTGCCCCCCTGGTGCCGACCGCTCAgg          >  2:4552435/1‑101 (MQ=37)
                                                                                                 tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt       >  2:3517660/1‑101 (MQ=255)
                                                                                                  cccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtg      >  2:776449/1‑101 (MQ=255)
                                                                                                   ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt     >  1:4164827/1‑101 (MQ=255)
                                                                                                    cGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGtt    >  2:3465881/1‑101 (MQ=255)
                                                                                                     ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt   >  2:3090006/1‑101 (MQ=255)
                                                                                                     ggTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGttt   <  2:493028/101‑1 (MQ=255)
                                                                                                      gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg  <  2:2087128/101‑1 (MQ=255)
                                                                                                      gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg  <  2:3621047/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
GGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCA.CCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTG  >  NC_014034/3003942‑3004142

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG