Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,024,766 Δ1 bp 100% intergenic (‑176/‑152) chvI ← / → pckA transcriptional regulator ChvI/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,024,7660T→.100.0% 1141.8 257intergenic (‑176/‑152)chvI/pckAtranscriptional regulator ChvI/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  new base (125/132):  ref base (0/0):  total (125/132)

Only 100 of 283 total aligned reads displayed.

TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCC  >  NC_014034/3024666‑3024861
                                                                                                    |                                                                                               
tgCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGt                                                                                                 >  2:3635512/1‑101 (MQ=255)
   gAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttg                                                                                              >  2:3645407/1‑100 (MQ=255)
    aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                             <  1:2744899/101‑3 (MQ=255)
      tCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgcgc                                                                                           >  2:1702607/1‑97 (MQ=255)
      tCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgcgc                                                                                           >  2:3016470/1‑97 (MQ=255)
       cGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCt                                                                                         >  2:1452681/1‑101 (MQ=255)
       cGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCt                                                                                         >  2:2376420/1‑101 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                        <  1:62484/101‑1 (MQ=255)
        gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                        <  1:4257820/101‑1 (MQ=255)
             cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                   >  2:2081164/1‑101 (MQ=255)
             cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                   <  1:3268372/101‑1 (MQ=255)
             cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                   <  2:3402124/101‑1 (MQ=255)
             cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                   <  1:1491145/101‑1 (MQ=255)
              gTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCg                                                                                  >  2:2960174/1‑101 (MQ=255)
              gTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.CTTGCGATAGCTGCg                                                                                  >  2:734067/1‑101 (MQ=255)
                 aTGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACa                                                                               >  1:252594/1‑101 (MQ=255)
                 aTGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACa                                                                               <  2:4627924/101‑1 (MQ=255)
                   gCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaaa                                                                             <  2:4535061/101‑1 (MQ=255)
                    cGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAAc                                                                            <  1:1142481/101‑1 (MQ=255)
                       atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                         >  2:945881/1‑101 (MQ=255)
                       atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                         <  1:1401296/101‑1 (MQ=255)
                         catcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTc                                                                       <  2:4749856/101‑1 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                      <  2:2844890/101‑1 (MQ=255)
                          atcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCt                                                                      <  1:2129652/101‑1 (MQ=255)
                            cTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGt                                                                    <  2:4137691/101‑1 (MQ=255)
                            cTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGt                                                                    >  1:4415202/1‑101 (MQ=255)
                              gAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTc                                                                  >  1:3196651/1‑101 (MQ=255)
                                 cgcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCtt                                                               <  1:1452775/101‑1 (MQ=255)
                                 cgcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCtt                                                               >  2:4169568/1‑101 (MQ=255)
                                 cgcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCtt                                                               <  2:2633276/101‑1 (MQ=255)
                                  gcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTc                                                              <  1:1069335/101‑1 (MQ=255)
                                  gcAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTc                                                              <  2:1925026/101‑1 (MQ=255)
                                   cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                             >  2:863701/1‑101 (MQ=255)
                                     aaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCa                                                           >  2:3045207/1‑101 (MQ=255)
                                     aaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCa                                                           >  1:3536912/1‑101 (MQ=255)
                                     aaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCa                                                           >  2:1405026/1‑101 (MQ=255)
                                      aaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCaa                                                          >  2:3713443/1‑101 (MQ=255)
                                       aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                         <  2:49257/101‑1 (MQ=255)
                                            gAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcgc                                                    >  2:3080310/1‑101 (MQ=255)
                                             aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGcc                                                   <  1:1282032/101‑1 (MQ=255)
                                                 tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                               <  1:2358134/101‑1 (MQ=255)
                                                   tGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGta                                             <  1:3045395/101‑1 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                            <  2:1251733/101‑1 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                            >  1:691077/1‑101 (MQ=255)
                                                    gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                            <  1:3713673/101‑1 (MQ=255)
                                                     aCTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtata                                           <  1:76789/101‑1 (MQ=255)
                                                     aCTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtata                                           <  1:4169814/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                          <  2:3851556/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                          <  1:2639895/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                          <  1:204979/101‑1 (MQ=255)
                                                      cTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAg                                          >  2:128954/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         >  2:3260542/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         >  1:3789264/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         >  2:2515913/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         >  1:465743/1‑101 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         <  1:4572580/101‑1 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         <  1:286166/101‑1 (MQ=255)
                                                       tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                         >  1:4469235/1‑101 (MQ=255)
                                                        aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                        >  1:3374934/1‑101 (MQ=255)
                                                         aTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgc                                       <  2:1744454/101‑1 (MQ=255)
                                                          tttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcg                                      >  1:1876349/1‑101 (MQ=255)
                                                          tttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcg                                      <  1:123614/101‑1 (MQ=255)
                                                            tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                    >  2:3441132/1‑101 (MQ=255)
                                                             gTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCa                                   <  2:222199/101‑1 (MQ=255)
                                                             gTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.ATTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCa                                   >  2:247145/1‑101 (MQ=255)
                                                              tctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCAt                                  >  1:847508/1‑101 (MQ=255)
                                                              tctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCAt                                  >  1:585099/1‑101 (MQ=255)
                                                               ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                 >  2:4455092/1‑101 (MQ=255)
                                                               ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                 <  1:3191988/101‑1 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                >  1:239021/1‑101 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                <  2:4718258/101‑1 (MQ=255)
                                                                tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGAAATTGCGCCATGGTATAGGGCGCCATcc                                >  2:427792/1‑101 (MQ=255)
                                                                 cACCTCTTCGCGATGATGTCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                               >  1:3638002/1‑101 (MQ=255)
                                                                      ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                          >  1:101282/1‑101 (MQ=255)
                                                                      ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                          <  2:2710777/101‑1 (MQ=255)
                                                                       ttCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAt                         >  2:3909850/1‑101 (MQ=255)
                                                                        tCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAtt                        <  2:3555016/101‑1 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                     >  1:1731176/1‑101 (MQ=255)
                                                                           cgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCtt                     <  1:4680061/101‑1 (MQ=255)
                                                                            gatgatGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTg                    >  1:4515348/1‑101 (MQ=255)
                                                                              tgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAACCCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcg                  <  2:1090078/101‑1 (MQ=255)
                                                                              tgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcg                  >  2:4373086/1‑101 (MQ=255)
                                                                              tgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcg                  <  2:1503953/101‑1 (MQ=255)
                                                                               gatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcgc                 >  2:165341/1‑101 (MQ=255)
                                                                               gatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgcgc                 >  1:3461424/1‑101 (MQ=255)
                                                                                  ggCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGa              >  2:4283388/1‑101 (MQ=255)
                                                                                   gcgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGAt             <  2:1822046/101‑1 (MQ=255)
                                                                                   gcgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGAt             <  1:971754/101‑1 (MQ=255)
                                                                                    cgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATc            <  2:3992000/101‑1 (MQ=255)
                                                                                    cgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATc            <  1:1983071/101‑1 (MQ=255)
                                                                                     gcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCg           <  2:1876237/101‑1 (MQ=255)
                                                                                     gcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCg           >  1:503090/1‑101 (MQ=255)
                                                                                     gcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCg           <  1:1574503/101‑1 (MQ=255)
                                                                                       tAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGt         >  1:2677696/1‑101 (MQ=255)
                                                                                         attCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgc       <  1:886347/99‑1 (MQ=255)
                                                                                         aaTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgc       <  1:2789903/101‑1 (MQ=255)
                                                                                          aTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcc      <  2:4515095/101‑1 (MQ=255)
                                                                                              gCCCTG.TTTGGGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc  <  2:1122694/101‑1 (MQ=255)
                                                                                              gCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc  >  2:394145/1‑101 (MQ=255)
                                                                                              gCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc  >  2:3506440/1‑101 (MQ=255)
                                                                                                    |                                                                                               
TGCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCC  >  NC_014034/3024666‑3024861

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG