Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,687 Δ1 bp 100% intergenic (+12/‑171) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6870G→.100.0% 89.5 149intergenic (+12/‑171)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (5/115):  ref base (27/0):  total (34/115)
Fisher's exact test strand distribution p-value = 8.49e-25
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.04e-14

Only 100 of 180 total aligned reads displayed.

ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTT  >  NC_014034/3254587‑3254779
                                                                                                     |                                                                                              
aCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACg                                                                                                <  2:3976478/101‑1 (MQ=255)
 cccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgg                                                                                               <  2:135406/101‑1 (MQ=255)
 cccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgg                                                                                               <  1:2130872/101‑1 (MQ=255)
    gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcgggg                                                                                            >  2:2552736/1‑101 (MQ=255)
    gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGGGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACagggg                                                                                            >  2:1775142/1‑101 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggga                                                                                           <  1:3415365/101‑2 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggga                                                                                           <  1:2333556/101‑2 (MQ=255)
        ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggg                                                                                         >  2:1340191/1‑99 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggggccgcc                                                                                      >  2:1267698/1‑96 (MQ=255)
           aTCGAGACCAAAACGATCCTGATGCGCTCGAAATCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccg                                                                                     <  2:395135/101‑8 (MQ=255)
            tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggggtgcccg                                                                                    >  1:1498307/1‑94 (MQ=255)
              gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggggtgccgccc                                                                                  >  1:1236464/1‑92 (MQ=255)
                gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tcccc                                                                             <  2:2085188/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGTTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tccccc                                                                            <  2:4671751/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tccccc                                                                            <  2:3076797/101‑1 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tccccc                                                                            <  1:2510061/101‑1 (MQ=255)
                   cGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGc                                                                          <  2:3255453/101‑1 (MQ=255)
                    gAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.CG.Gccccccc                                                                          >  1:559067/1‑99 (MQ=255)
                    gAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCGTCC.CG.Cccccccc                                                                          >  2:2271971/1‑99 (MQ=255)
                    gAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCa                                                                         <  1:3010059/101‑1 (MQ=255)
                      aaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGC.CG.Ccccccccca                                                                        >  1:2774628/1‑97 (MQ=255)
                         gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCAccccc                                                                    <  1:950470/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCt                                                                   <  2:29243/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCt                                                                   <  1:3178083/101‑1 (MQ=255)
                           tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTg                                                                  <  2:2854529/101‑1 (MQ=255)
                              tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCTCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                               <  2:4118796/101‑1 (MQ=255)
                              tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                               <  1:3767276/101‑1 (MQ=255)
                              tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                               <  2:4525991/101‑1 (MQ=255)
                                 tGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCCCACCCCCCGcgcgca                                                            >  2:656414/1‑99 (MQ=255)
                                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CC.TCCCCCCCCCCCCCCCCGCGGAt                                                           >  1:598670/1‑101 (MQ=255)
                                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAt                                                           <  1:3682823/101‑1 (MQ=255)
                                     cTCGAAGACCGGCTCGGTGCGCCGGGTGATCTATCGCAGCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAtttt                                                        <  2:2881872/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAtttt                                                        <  2:1640274/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAtttt                                                        <  1:3895726/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAtttt                                                        <  1:2111013/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAtttt                                                        <  2:137002/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTAtttt                                                        <  1:4652907/101‑1 (MQ=255)
                                      tCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTg                                                       <  2:3010184/101‑1 (MQ=255)
                                      tCGAAAACCGGCTCGGGGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTg                                                       <  2:3724222/101‑1 (MQ=255)
                                       cGAAACCCGGCTCGGCGCGCCGGTTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTTCGCGTATTTTGc                                                      <  1:2564532/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGA.GC.CC.TCCCCCCCACCCCCCCCGCGAAttttttca                                                    >  1:690805/1‑97 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                    <  1:1666886/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                    <  1:2840671/101‑1 (MQ=255)
                                           aaCCAGATCGGTGCGCCGGTCGATCTATCCCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGG.CG.TCCCCCGCACGCCCTGCGCGTATTTTGCCAAg                                                  <  1:2691207/101‑1 (MQ=255)
                                            aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAgt                                                 <  2:647870/101‑2 (MQ=255)
                                              cGGCTCGGTGCGCCGGGTGATCTATCACAACCCGATCCGCTAAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaa                                               <  2:4717406/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCCGCGCGTATTTTGCCAAGGaaa                                              >  2:177108/1‑101 (MQ=37)
                                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                             <  1:66760/101‑1 (MQ=38)
                                                 cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAAc                                            <  2:3394825/101‑1 (MQ=38)
                                                 cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAAc                                            <  1:4113794/101‑1 (MQ=38)
                                                 cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAAc                                            <  2:640543/101‑1 (MQ=38)
                                                  tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACg                                           <  1:83113/101‑1 (MQ=38)
                                                  tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACg                                           <  1:3741047/101‑1 (MQ=38)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CC.CCCCCCCCCCCCCCCCCGCGGCTTTTTCCCAAGAAAACcgg                                         >  2:3528723/1‑101 (MQ=255)
                                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACgggg                                        <  2:4137638/101‑1 (MQ=38)
                                                        cgcCGGGTGATCTATCGCACCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                     <  2:4584240/101‑1 (MQ=38)
                                                        cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                     <  2:515518/101‑1 (MQ=38)
                                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAGAACGGGGCCGtt                                   <  1:2562238/101‑1 (MQ=38)
                                                           ctggTGGTCTATAGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGttt                                  <  2:4559534/99‑1 (MQ=37)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGttt                                  <  1:2052454/101‑1 (MQ=38)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGttt                                  <  1:3474825/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTc                                 <  2:996590/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTc                                 <  1:1053770/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTc                                 <  1:422810/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CG.GCCCCCCCCCCCCCCCCCCCGCTTTTTGCAACGAAAACCGGGCCGCTTcc                                >  1:1440953/1‑100 (MQ=255)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCa                                <  1:3257976/101‑1 (MQ=38)
                                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CGGC.CC.GCCCCCCCCCCCCCCCCCCGGGTTTTTCCAAAGAAAAAGGGGCCGGTTCAc                               >  1:2849970/1‑101 (MQ=255)
                                                               tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GCGC.CG.TCCCCCGCACCCCCCCAGCGGGTTTTTTCCAAGAAAAAGGGGCCGgtttccc                              >  2:2763882/1‑97 (MQ=16)
                                                                 aTCTATCGCACCCCGAGCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCTCACCCCCGGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                            <  2:4158077/101‑1 (MQ=37)
                                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                            <  1:491737/101‑1 (MQ=38)
                                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTAACCtt                            <  2:1598416/101‑1 (MQ=38)
                                                                   cTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCGCC.CCCCCCCCCCCCCCCCCCCGT.TTTTTCCAAGAAAAAAAGGGCGGTTTCCCCtcg                           >  1:2588366/1‑99 (MQ=255)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGG.CG.TCCCCCCCCCCCCCCGCGCGGCTTTTTCCAAGGAAAAAGGGGCCGGTTCACCCTTGcc                        >  1:1575778/1‑101 (MQ=255)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGC.CG.TCCCCCCCCCCCCCCCCCCGGGTTTTTCCCAGGAAAACAGGGGCGttttccccttgcc                        >  1:2288308/1‑91 (MQ=255)
                                                                        gCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGaa                     <  2:3688275/101‑1 (MQ=38)
                                                                         cAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CA.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGaaa                    <  1:3918612/101‑1 (MQ=38)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                   <  1:1147643/101‑1 (MQ=38)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                   <  1:965982/101‑1 (MQ=38)
                                                                            cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAatat                 <  2:2288520/101‑1 (MQ=38)
                                                                             ccGATCCGCTGAGGCAACGCT.ACGGGGGGCTGGC.GGCCCCCCCCCCCCCCCCCCGCGT.TTTTTCAACGAAAAAAGGGGCGCTTTTCCCTTCGCCGaacatc                 >  2:4610320/1‑97 (MQ=255)
                                                                             ccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATc                <  2:1911809/101‑1 (MQ=38)
                                                                             ccGATCCGCTGAGGCAACGCT.AC.GGGGGACCGT.CG.TTCCCCCCACCCCCCGCGCGTATTTTGCTCAGGAAAACGGGGGCGTTTCAAGTTGGCCGAAAGATc                >  2:3168173/1‑101 (MQ=255)
                                                                              cGATCCGCTGAGGCAACGCT.AC.GGGAGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATcc               <  1:3123682/101‑1 (MQ=38)
                                                                               gATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.CG.CCCCCCCCCCCCCCCCCCCCGCTTTTTCCAGGAAAAAAGGGGCGGGTTACCCTTGGCGGaaaaaccc               >  2:2480976/1‑96 (MQ=255)
                                                                                aTCCGCTGAGGCAACGCT.ACGGGGGGGGGCC.GG.CCCCCCCCCCCCCCCCCCCGGTTTTTTCCAGGAAAAACGGGGCGGTTTCCCCTTGGCCGaaaaaaacc              >  2:1052060/1‑95 (MQ=255)
                                                                                aTCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CC.GCCCCCCCACCCCCCGCGCGCATTTTGTCAAAGAAAAACGGGCCGTTTCACCCTGGGCGAAAAATTCCg             >  2:768630/1‑101 (MQ=255)
                                                                                 tCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CG.CCCCCCGCCCCCCCCCCGCGCCCCTTTCCAAGGAAACCGNGGCCGGCTCCCCTTGGCCGCCATATcaccg            >  1:5028/1‑97 (MQ=255)
                                                                                   cGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgggg          <  1:1340283/101‑1 (MQ=38)
                                                                                      tGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggggggg       <  1:656478/101‑1 (MQ=38)
                                                                                      ggAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggggggg       <  1:959614/100‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa      <  2:2849794/101‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa      <  1:478458/101‑1 (MQ=38)
                                                                                        aGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGaa     <  1:4369752/101‑1 (MQ=38)
                                                                                         ggCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAt    <  2:2726990/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGCCTGC.CG.TCCCCCGCCCCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt   <  1:4601175/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt   <  1:415113/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt   <  2:1498206/101‑1 (MQ=38)
                                                                                          gCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAtt   <  2:2149260/101‑1 (MQ=38)
                                                                                           cAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGGGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt  <  1:3307252/101‑1 (MQ=37)
                                                                                           cAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt  <  1:1944583/101‑1 (MQ=38)
                                                                                                     |                                                                                              
ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGC.CG.TCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTT  >  NC_014034/3254587‑3254779

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG