Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,791 Δ1 bp 2.6% intergenic (‑94/+69) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,7910G→. 2.6% 3.8 0.0 0.0154intergenic (‑94/+69)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (70/80):  total (72/82)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

CTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCG  >  NC_014034/3439693‑3439893
                                                                                                    |                                                                                                              
   aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.AA.AAAa.ggggc                                                                                                          >  1:3447652/1‑99 (MQ=255)
   aTGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGgct                                                                                                          <  1:920583/101‑1 (MQ=255)
    tGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGTCGGGGATATTTGGGGCAAG.AT.AAAG.GGgctt                                                                                                         <  1:4213589/101‑1 (MQ=255)
     ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAG.AG.AAAA.GGgcttt                                                                                                        >  1:2443126/1‑101 (MQ=255)
     ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGgcttt                                                                                                        <  2:1997825/101‑1 (MQ=255)
     ggcggcGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGgcttt                                                                                                        <  2:1542403/101‑1 (MQ=255)
         gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.TGgctttgctt                                                                                                    <  2:4387799/101‑1 (MQ=255)
         gcgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGgctttgctt                                                                                                    <  2:2285727/101‑1 (MQ=255)
          cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTTGGGGAG.AT.TAAG.GGgggttgtttt                                                                                                   >  1:4409302/1‑101 (MQ=255)
          cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGGAG.AA.AAAA.GGgggtttcttt                                                                                                   >  1:90332/1‑101 (MQ=255)
          cgACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGgctttgcttt                                                                                                   <  2:565451/101‑1 (MQ=255)
           gACCCTACACGGCGCGATCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGTATATTTGGGGCAAT.AT.AAAG.GGGCTTTGCtttt                                                                                                  <  2:4701140/101‑1 (MQ=255)
           gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCtttt                                                                                                  <  1:2768129/101‑1 (MQ=255)
           gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCtttt                                                                                                  <  1:3756477/101‑1 (MQ=255)
           gACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCtttt                                                                                                  <  2:1467369/101‑1 (MQ=255)
            aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTa                                                                                                 <  2:3902816/101‑1 (MQ=255)
            aCCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTa                                                                                                 <  1:106417/101‑1 (MQ=255)
             cccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.AT.AAAA.GGGCTTTTCtttttt                                                                                                >  1:4322022/1‑99 (MQ=255)
              ccTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATc                                                                                               <  1:1429714/101‑1 (MQ=255)
               cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.t                                                                                             <  1:3289006/101‑1 (MQ=255)
               cTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.t                                                                                             <  1:1614897/101‑1 (MQ=255)
                ttcacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.tt                                                                                            <  1:260332/99‑1 (MQ=255)
                tACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGAATTTGGGCACA.ATAAAAG.GGGGTTTCCtttttttt                                                                                              >  1:3052006/1‑97 (MQ=255)
                 acacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGGAG.GT.AAAA.GGGGGTTGTTTTTTTT.ttt                                                                                           >  1:1267356/1‑101 (MQ=255)
                  cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTc                                                                                          <  1:804986/101‑1 (MQ=255)
                  cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTc                                                                                          <  2:1403482/101‑1 (MQ=255)
                  cacGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTCCGGCCGAGGCCGCCGGCGGCGACGTTTGGGGCGAA.AA.AAAGAGGGGATTG.GTTTATC.TTTc                                                                                          >  1:4717750/1‑101 (MQ=255)
                   acGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTc.c                                                                                        <  2:242329/101‑1 (MQ=255)
                    cGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.AT.AAAA.GGGCGTTGCTTTTTTT.TTTt.cc                                                                                       >  2:272772/1‑101 (MQ=255)
                     ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTc.ccc                                                                                      <  2:4184132/101‑1 (MQ=255)
                     ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTc.ccc                                                                                      <  2:3361681/101‑1 (MQ=255)
                     ggCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTc.ccc                                                                                      <  2:1898779/101‑1 (MQ=255)
                      gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCa                                                                                     <  1:1358401/101‑1 (MQ=255)
                      gcgcGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCa                                                                                     <  2:1797897/101‑1 (MQ=255)
                        gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.AT.AAAG.GGGGGTTGCTTTTTTC.TTTT.CCCc.aa                                                                                  >  2:1129551/1‑101 (MQ=255)
                        gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCa.aa                                                                                  <  1:1851855/101‑1 (MQ=255)
                        gcgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCa.aa                                                                                  <  1:435331/101‑1 (MQ=255)
                         cgtccAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.Aat                                                                                 <  2:3858745/98‑1 (MQ=255)
                         cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTTGGGCCAGAAA.AAAG.GGGTTTTTTTTTTTTT.TTTC.CCCa.aa                                                                                  >  2:3696712/1‑101 (MQ=255)
                         cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.Aat                                                                                 <  2:2765727/101‑1 (MQ=255)
                         cgACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.Aat                                                                                 <  1:3446376/101‑1 (MQ=255)
                          gatcagggtgcGAAGGACAGGAATTCGGTCTGCGGGCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGATTTGCTTTTATC.TTTC.CCCA.Aata                                                                                <  2:2191486/91‑1 (MQ=25)
                          gACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.AT.AAAA.GGGGGTTTCTTTTTTT.tttttccacaaa                                                                                  >  2:3275941/1‑92 (MQ=255)
                           aCCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.Aata.t                                                                              <  1:1020627/101‑1 (MQ=255)
                             cAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.Tcc                                                                            <  2:4095267/101‑1 (MQ=255)
                              aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.Tccc                                                                           <  2:54275/101‑1 (MQ=255)
                              aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.Tccc                                                                           <  2:1671344/101‑1 (MQ=255)
                              aGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.Tccc                                                                           <  1:2957052/101‑1 (MQ=255)
                                  ttCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCgggg                                                                       <  2:692218/101‑1 (MQ=255)
                                    cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAA.AA.AAAG.GGGAGTTGCTTTTTTCTTTTC.CCCA.AAAT.TCCCggggg                                                                      >  1:1573614/1‑101 (MQ=255)
                                    cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.AT.AAAA.GGGGTTTGGTTTTTTT.TTTT.TCCC.GATA.TCCCcggggg                                                                     >  1:2839846/1‑100 (MQ=255)
                                    cGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGAATTTTGGGCGAG.GA.AAAA.GGGCTTTGCTTTTTTT.TTTT.CCCC.AAAA.TCCCcggggg                                                                     >  2:4246556/1‑100 (MQ=255)
                                      aaaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCACG.AT.AAAG.GGGCATTGCTTTTTTC.TTTC.CCCA.AAGA.TCCCCGGGGGGc                                                                   >  1:2984448/1‑101 (MQ=37)
                                       aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.ATAAAAG.GGGTTTTTTTTTTTTT.TTTT.CCAA.AAAT.TCCCgggggggg                                                                   >  1:4555445/1‑98 (MQ=255)
                                       aaGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.AT.AAAA.GGGGTTTGCTTTTTTC.TTTT.CCCA.AAAA.TCCCggggggggg                                                                  >  1:4641847/1‑97 (MQ=255)
                                        aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.AT.AAAA.GGGGTTTTCTTTTTTT.TTTT.CCCC.AAAA.TCCCCGGGGGGC..gg                                                               >  2:2502529/1‑101 (MQ=255)
                                          aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..gg.gg                                                            <  1:3507544/101‑1 (MQ=255)
                                             ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTTGGGGAAG.AT.AAAA.GGGGTTTGCTTTTATT.TTTC.CCCC.AAAA.TTCCGGGGGGGC..GG.GGGCa                                                         >  2:2954947/1‑101 (MQ=255)
                                             ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCa                                                         <  1:2013312/101‑1 (MQ=255)
                                             ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCa                                                         <  2:1267265/101‑1 (MQ=255)
                                              gAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCGGG.AG.AAAG.GGGGTTTTTTTTTTTC.TTTT.CCCA.ACTA.TTCCCGGGGTGG..GG.GGGCag                                                        >  1:2296951/1‑101 (MQ=255)
                                               aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGGAG.GG.AAAA.GGGGGTTGTTTTTTTT.TTTCTCCCACAATT.CCCCCGGGGGGG..gg.ggggg                                                         >  2:1646453/1‑99 (MQ=255)
                                                aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAG.AT.AAAA.GGGGTTTGGTTTTTTT.TTTT.CCCC.AATATTCCCGGGGGGGG..gg.gggggag                                                       >  1:320001/1‑97 (MQ=255)
                                                aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCagag                                                      <  1:272806/101‑1 (MQ=255)
                                                aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCagag                                                      <  2:258922/101‑1 (MQ=255)
                                                   cGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAG.ATAAAAG.GGGTTTGGTTTTTTTT.TTTC.CCCA.AAAT.TCCCGGGGG.GC..gg.gggggggggcc                                                   >  2:4071818/1‑93 (MQ=255)
                                                    ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAG.AT.AAAG.GGGCTTTGCTTTTATT.TTTC.CCCA.AATA.TCCCGGGGGGGC..GG.GGGGGGAGgccc                                                  >  2:2183387/1‑101 (MQ=25)
                                                       tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.GAA..ACCCATCCCTTTCATC.TTGC.CCCA.AATA.TCCCGGGGGGCCCGGG.GGGCAGCGcccccc                                                <  2:2970950/94‑2 (MQ=25)
                                                          cGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAG.AT.AAAG.GGGGTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGGGC..GG.GGGGAGAGCCCCcgccgc                                            >  2:2804717/1‑98 (MQ=21)
                                                           ggCCGATGCCTCCGGCGGGGATATTTGGGGGAAG.AT.AAAG.GGGGTTTGCTTTTATC.TTTT.CCCC.AATA.TCCCCGGGGTGC..GG.GGGGCGAGccccccccgcc                                           >  2:1221718/1‑95 (MQ=25)
                                                              cGATGCCTCCGGCGGGGACATTTGGGGGAAG.AT.GAA..ACCCATCCCTTTCTTC.TTTC.CCCC.AATATTCCAGGGGGCCC..GGTGGGCAGAGcccccccccactt                                         >  2:2913279/1‑93 (MQ=16)
                                                               gATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGGTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCCGGGGGGC..GG.GGGCAGAGCCCC..CGCCGCCACgg                                     >  2:2625519/1‑101 (MQ=18)
                                                                 tGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCCcgccgccccggtt                                     >  2:2312820/1‑91 (MQ=255)
                                                                         gcggGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCCcgccgc                           >  2:1286549/1‑101 (MQ=255)
                                                                         gcggGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                           >  1:3249207/1‑101 (MQ=255)
                                                                          cggGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                          >  2:677272/1‑101 (MQ=255)
                                                                            gggATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                        >  2:3320930/1‑101 (MQ=255)
                                                                             ggATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                       >  1:3941347/1‑101 (MQ=255)
                                                                             ggATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                       >  2:4303726/1‑101 (MQ=255)
                                                                                 atTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                   >  1:2535828/1‑101 (MQ=255)
                                                                                        gCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggggc            >  1:3890313/1‑100 (MQ=255)
                                                                                        gCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt            >  1:2058238/1‑101 (MQ=255)
                                                                                         cAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc           >  2:2796296/1‑101 (MQ=255)
                                                                                         cAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc           >  2:3104573/1‑101 (MQ=255)
                                                                                          aaG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg          >  1:1329299/1‑101 (MQ=255)
                                                                                          aaG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg          >  1:1460683/1‑101 (MQ=255)
                                                                                          aaG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg          >  2:1361508/1‑101 (MQ=255)
                                                                                          aaG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg          >  1:2398964/1‑101 (MQ=255)
                                                                                           aG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGc         >  2:3502780/1‑101 (MQ=255)
                                                                                              aT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  1:611880/1‑101 (MQ=255)
                                                                                              aT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  1:1833567/1‑101 (MQ=255)
                                                                                              aT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa       >  2:652420/1‑101 (MQ=255)
                                                                                               t.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg      >  2:3608486/1‑101 (MQ=255)
                                                                                               t.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAg      >  2:1708134/1‑101 (MQ=255)
                                                                                                 aaaG.TGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGGGC..GG.GGGCAGAGACTC..CGCCGCCACGGTTCACGCCGCGACCGCCTCCGGGGTCGCCAgc     >  2:4017047/1‑101 (MQ=37)
                                                                                                 aaaG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCTGGTGGCAC..CG.GGACAGAGCCCCCG.G.CGCCCCGGCTCACGCCGCGACCGCCTCCAGAGACGCCAgc     >  1:796877/1‑101 (MQ=255)
                                                                                                 aaaG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc     >  1:2939268/1‑101 (MQ=255)
                                                                                                  aaG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg    >  2:442266/1‑101 (MQ=255)
                                                                                                  aaG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg    >  2:3770490/1‑101 (MQ=255)
                                                                                                  aaG.AGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg    >  2:4022928/1‑101 (MQ=255)
                                                                                                    |                                                                                                              
CTCATGGCGGCGACCCTACACGGCGCGACCAGCTTTCGAAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAG.AT.AAAG.GGGCTTTGCTTTTATC.TTTC.CCCA.AATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCG  >  NC_014034/3439693‑3439893

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG