Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,846 Δ1 bp 100% intergenic (‑149/+14) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8460C→.100.0% 570.6 132intergenic (‑149/+14)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (132/0):  ref base (0/0):  total (132/0)

Only 100 of 170 total aligned reads displayed.

GCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGACC  >  NC_014034/3439746‑3439944
                                                                                                         |                                                                                                  
gCCTGCGGCCGATGCCTCCGGCGGGGATATTTTGGGGAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCCGGGGGGC..GG.GGGCAGAGccccc                                                                                                    >  2:3820929/1‑101 (MQ=25)
 gtgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTTA.TCTTTCCCCCAATA.TCCCGGGGGGCCCGGG.GGGCAGCGccccc                                                                                                    >  2:544815/9‑101 (MQ=21)
  tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAA.ACCCATCCCTTTCA.TCTTGCCCCAAATA.TCCCGGGGGGCCCGGG.GGGCAGCGcccccc                                                                                                   <  2:2970950/94‑2 (MQ=25)
   tGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAAGGGGGTTGTTTTTATTTTTTCCCCAAAAT.TCCCGGGGGGGG..GG.GGGC.GAGcccccccc                                                                                                 >  1:1253247/1‑98 (MQ=255)
    gCGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGGGTTGCTTTTA.TCTTTCCCCAAATA.TCCCCGGGGTGC..GG.GGGCAGAGGCCCcgccc                                                                                                >  2:4251905/1‑99 (MQ=21)
     cGGCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGGTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGGGC..GG.GGGGAGAGCCCCcgccgc                                                                                               >  2:2804717/1‑98 (MQ=21)
      ggCCGATGCCTCCGGCGGGGATATTTGGGGGAAGATAAAGGGGGTTTGCTTTTA.TCTTTTCCCCAATA.TCCCCGGGGTGC..GG.GGGGCGAGccccccccgcc                                                                                              >  2:1221718/1‑95 (MQ=25)
         cGATGCCTCCGGCGGGGACATTTGGGGGAAGATGAA.ACCCATCCCTTTCT.TCTTTCCCCCAATATTCCAGGGGGCCC..GGTGGGCAGAGcccccccccactt                                                                                            >  2:2913279/1‑93 (MQ=16)
            tGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCCcgccgccccggtt                                                                                        >  2:2312820/1‑91 (MQ=255)
             gCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGGTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTc                                                                                     >  1:4582512/1‑101 (MQ=255)
                  cggaggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCAcgcc                                                                                >  2:4140269/1‑101 (MQ=255)
                    gcggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCCcgccgc                                                                              >  2:1286549/1‑101 (MQ=255)
                    gcggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                                                              >  1:1448653/1‑101 (MQ=255)
                    gcggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                                                              >  1:3249207/1‑101 (MQ=255)
                     cggGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCcgcg                                                                             >  2:677272/1‑101 (MQ=255)
                      ggggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                                                            >  2:793766/1‑101 (MQ=255)
                        ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                                                          >  2:4303726/1‑101 (MQ=255)
                        ggATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                                                          >  2:2794342/1‑101 (MQ=255)
                         gATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                                                                         >  2:2529555/1‑101 (MQ=255)
                            atTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                                                      >  2:3617320/1‑101 (MQ=255)
                             tttGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                                                                     >  2:679157/1‑101 (MQ=255)
                               tGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                                   >  1:804264/1‑101 (MQ=255)
                               tGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                                   >  2:2428002/1‑101 (MQ=255)
                               tGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                                   >  1:4667382/1‑101 (MQ=255)
                                ggggCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                                                                  >  1:1496583/1‑101 (MQ=255)
                                 gggCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                                                                 >  1:1963353/1‑101 (MQ=255)
                                   gCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggggc                                                               >  1:3890313/1‑100 (MQ=255)
                                   gCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                                                               >  1:2058238/1‑101 (MQ=255)
                                    cAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                                                              >  1:25421/1‑101 (MQ=255)
                                    cAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                                                              >  2:3104573/1‑101 (MQ=255)
                                     aaGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                             >  1:1329299/1‑101 (MQ=255)
                                     aaGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                             >  2:1361508/1‑101 (MQ=255)
                                     aaGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                             >  2:1708896/1‑101 (MQ=255)
                                     aaGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                             >  1:2398964/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGccg                                                          >  1:4131977/1‑100 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  2:4647566/1‑101 (MQ=255)
                                        aTAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                                          >  2:652420/1‑101 (MQ=255)
                                         tAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                         >  2:2618055/1‑101 (MQ=255)
                                         tAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                         >  1:1556321/1‑101 (MQ=255)
                                         tAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                         >  1:3264867/1‑101 (MQ=255)
                                         tAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                                         >  2:3608486/1‑101 (MQ=255)
                                          aaaGTGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGGGC..GG.GGGCAGAGACTC..CGCCGCCACGGTTCACGCCGCGACCGCCTCCGGGGTCGCCAgc                                                        >  2:4017047/1‑101 (MQ=37)
                                          aaaGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgc                                                        >  1:2939268/1‑101 (MQ=255)
                                          aaaGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgc                                                        >  1:860870/1‑101 (MQ=255)
                                           aaGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                                       >  2:442266/1‑101 (MQ=255)
                                           aaGAGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                                       >  2:4022928/1‑101 (MQ=255)
                                             ggggCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGcgcg                                                     >  1:2433957/1‑101 (MQ=255)
                                                 ctttgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga                                                 >  2:3368405/1‑101 (MQ=255)
                                                  tttgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat                                                >  1:4254429/1‑101 (MQ=255)
                                                   ttgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                               >  2:3901260/1‑101 (MQ=255)
                                                   ttgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                               >  1:325877/1‑101 (MQ=255)
                                                   ttgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                               >  1:4189790/1‑101 (MQ=255)
                                                   ttgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCAGGGTCGCCAGCGCGACGata                                               >  1:3964185/1‑101 (MQ=255)
                                                    tgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                                              >  2:1453038/1‑101 (MQ=255)
                                                    tgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                                              >  2:1638016/1‑101 (MQ=255)
                                                    tgctttTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatac                                              >  2:1387593/1‑100 (MQ=255)
                                                       ttttA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCca                                           >  1:4255232/1‑101 (MQ=255)
                                                         ttA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  2:4677508/1‑101 (MQ=255)
                                                         ttA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  2:2692151/1‑101 (MQ=255)
                                                         ttA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  2:376332/1‑101 (MQ=255)
                                                         ttA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                                         >  1:517427/1‑101 (MQ=255)
                                                         ttA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAGCGCGACGATATCcatc                                         >  2:858285/1‑101 (MQ=255)
                                                          tA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca                                        >  1:915285/1‑101 (MQ=255)
                                                           a.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatcat                                       >  1:3606262/1‑101 (MQ=255)
                                                             tCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATg                                      >  1:4403023/1‑101 (MQ=255)
                                                              cTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                                     >  2:2763163/1‑101 (MQ=255)
                                                              cTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                                     >  1:2511871/1‑101 (MQ=255)
                                                               tttCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGAt                                    >  2:1682982/1‑101 (MQ=255)
                                                               tttCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGAt                                    >  2:4496986/1‑101 (MQ=255)
                                                                ttCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATTATGATc                                   >  1:595708/1‑101 (MQ=255)
                                                                ttCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGGTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATc                                   >  2:2130170/1‑101 (MQ=255)
                                                                 tCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATcc                                  >  2:3240929/1‑101 (MQ=255)
                                                                 tCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATcc                                  >  2:1999069/1‑101 (MQ=255)
                                                                  ccccAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCg                                 >  2:2825505/1‑101 (MQ=255)
                                                                     cAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                              >  2:1556061/1‑101 (MQ=255)
                                                                      aaaTA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                             >  1:110725/1‑101 (MQ=255)
                                                                      aaaTA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGACATCCATCATGATCCGGTTc                             >  2:4739759/1‑101 (MQ=255)
                                                                       aaTA.TCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCa                            >  1:1665317/1‑101 (MQ=255)
                                                                        ata.tCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg                           >  1:878215/1‑101 (MQ=255)
                                                                         ta.tCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCTAGCGCGACGATATCCATCATGATCCGGTTCAGc                          >  1:4086448/1‑101 (MQ=255)
                                                                         ta.tCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc                          >  1:591546/1‑101 (MQ=255)
                                                                            tCCCGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc                        >  1:1039126/1‑101 (MQ=255)
                                                                               cGGGGGTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaa                     >  1:329911/1‑101 (MQ=255)
                                                                                gggggTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaaa                    >  1:270664/1‑101 (MQ=255)
                                                                                   ggTGC..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATcc                 >  1:1896228/1‑101 (MQ=255)
                                                                                       c..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg             >  2:1455994/1‑101 (MQ=255)
                                                                                       c..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg             >  1:798616/1‑101 (MQ=255)
                                                                                       c..GG.GGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg             >  1:3014565/1‑101 (MQ=255)
                                                                                          gg.gggCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCgg            >  2:1836149/1‑101 (MQ=255)
                                                                                          gg.gggCAGAGCCCC..CGCCGCAACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGACATCCATCATGATCCGGTTTAGCTCGAAATCCTTCgc            >  2:3895417/1‑100 (MQ=255)
                                                                                             gggCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCg          >  1:2845103/1‑101 (MQ=255)
                                                                                               ggcgcccccccGTCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCg          <  1:3012984/95‑1 (MQ=255)
                                                                                               gccccccccccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCg          <  2:3917698/95‑1 (MQ=255)
                                                                                                  ccccccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGtat       <  1:2763327/98‑1 (MQ=255)
                                                                                                    gagcccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAg     >  1:3757989/4‑101 (MQ=255)
                                                                                                    ccccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAg     <  1:4497236/100‑1 (MQ=255)
                                                                                                    ccccccCCCCCGCCACGGTTCACGCCGCGACCGGCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAg     <  1:652484/100‑1 (MQ=255)
                                                                                                     cccccCCCCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGa    <  2:1329207/101‑1 (MQ=255)
                                                                                                       cccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAcc  >  2:507294/1‑101 (MQ=255)
                                                                                                       cccCCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGAcc  >  1:1569072/1‑101 (MQ=255)
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GCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGATAAAGGGGCTTTGCTTTTA.TCTTTCCCCAAATA.TCCCGGGGGTGC..GG.GGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCGGCGTATAGACC  >  NC_014034/3439746‑3439944

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG