Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 549,629 Δ1 bp 100% coding (470/471 nt) greA → transcription elongation factor GreA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034549,6290A→.100.0% 866.9 197coding (470/471 nt)greAtranscription elongation factor GreA
Reads supporting (aligned to +/- strand):  new base (88/108):  ref base (0/0):  total (89/108)

Only 100 of 220 total aligned reads displayed.

TGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTAAAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCA  >  NC_014034/549530‑549725
                                                                                                   |                                                                                                
tGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaa                                                                                                 <  2:367415/101‑1 (MQ=255)
tGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaa                                                                                                 <  1:3043622/101‑1 (MQ=255)
tGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaa                                                                                                 <  1:2941988/101‑1 (MQ=255)
 ggCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaag                                                                                                <  1:4742413/101‑2 (MQ=255)
 ggCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaag                                                                                                <  2:3300077/101‑2 (MQ=255)
   cccGCGCGCTGATCGGCAAGGACGAATGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagcg                                                                                              >  1:3321046/1‑98 (MQ=255)
   cccGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTaagcg                                                                                              <  1:1933008/101‑4 (MQ=255)
     cgcgcgCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCggg                                                                                           >  1:1503253/1‑101 (MQ=255)
      gcgcgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGc                                                                                          >  1:1044407/1‑101 (MQ=255)
         cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcccc                                                                                       <  1:4553492/101‑1 (MQ=255)
         cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcccc                                                                                       <  2:3605396/101‑1 (MQ=255)
         cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGcccc                                                                                       <  2:1215752/101‑1 (MQ=255)
         cgcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTG.CAAGCGGGcccc                                                                                       >  2:21642/1‑101 (MQ=255)
          gcTGATCGGCGAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGccccc                                                                                      <  1:3479688/101‑1 (MQ=255)
          gcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGccccc                                                                                      <  1:1981598/101‑1 (MQ=255)
          gcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGccccc                                                                                      >  2:3500202/1‑101 (MQ=255)
          gcTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGccccc                                                                                      >  2:2960932/1‑101 (MQ=255)
           cTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCg                                                                                     <  2:1565596/101‑1 (MQ=255)
           cTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCg                                                                                     >  1:4125023/1‑101 (MQ=255)
           cTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCg                                                                                     >  1:732836/1‑101 (MQ=255)
           cTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGGCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCg                                                                                     >  1:3893038/1‑101 (MQ=255)
            tGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGa                                                                                    >  2:1781954/1‑101 (MQ=255)
            tGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGa                                                                                    <  1:2407130/101‑1 (MQ=255)
             gATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGAc                                                                                   <  1:2493360/101‑1 (MQ=255)
             gATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGAc                                                                                   <  2:2871422/101‑1 (MQ=255)
              aTCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGAcc                                                                                  <  1:1796533/101‑1 (MQ=255)
               tCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCa                                                                                 <  1:3777871/101‑1 (MQ=255)
                cGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAACCCTGTCGGTCCGTTTCCTGT.AAGCGGGCACCCGACCaa                                                                                >  2:4657686/1‑101 (MQ=255)
                  gCAAGGACGAAGGCGACAGCGTCGAGGTCCCCAGCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAgg                                                                              <  2:2451800/101‑1 (MQ=255)
                   cAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGa                                                                             <  1:1085873/101‑1 (MQ=255)
                   cAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGa                                                                             <  1:1064890/101‑1 (MQ=255)
                    caGGACGAAGGCGACAGCCGCGAGGGCCGCCCCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGAt                                                                            <  2:1275229/100‑1 (MQ=255)
                    aaGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGCCCAAGGAt                                                                            >  1:1359706/1‑101 (MQ=255)
                    aaGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGAt                                                                            >  1:447153/1‑101 (MQ=255)
                     aGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATg                                                                           <  1:1645940/101‑1 (MQ=255)
                      ggccggaGGCGACAGCGTCCAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGCGCCCCCGACCAAGGAGGa                                                                          <  2:2201970/95‑1 (MQ=255)
                      ggACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGa                                                                          <  2:424856/101‑1 (MQ=255)
                       gACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGAc                                                                         <  1:2123525/101‑1 (MQ=255)
                          gAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACgag                                                                      >  1:3258331/1‑101 (MQ=255)
                          gAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACgag                                                                      >  1:1307031/1‑101 (MQ=255)
                          gAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACgag                                                                      >  2:3211679/1‑101 (MQ=255)
                          gAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACgag                                                                      >  1:3877610/1‑101 (MQ=255)
                           aaGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACgaga                                                                     >  1:199693/1‑101 (MQ=255)
                            aGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGAt                                                                    <  1:4657942/101‑1 (MQ=255)
                              gCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATgg                                                                  <  1:3145459/101‑1 (MQ=255)
                              gCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATgg                                                                  <  1:1710671/101‑1 (MQ=255)
                               cGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATggg                                                                 <  1:1596372/101‑1 (MQ=255)
                                  cAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGa                                                              <  1:2601745/101‑1 (MQ=255)
                                       tCGAGGTCCGCCCCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGcccc                                                         <  2:1306939/101‑1 (MQ=255)
                                       tCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGcccc                                                         <  2:1692872/101‑1 (MQ=255)
                                       tCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGcccc                                                         <  1:2326921/101‑1 (MQ=255)
                                        cGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCa                                                        <  1:965206/101‑1 (MQ=255)
                                         gAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAg                                                       >  2:1410724/1‑101 (MQ=255)
                                         gAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAg                                                       >  1:13154/1‑101 (MQ=255)
                                            gTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTcc                                                    <  1:3211880/101‑1 (MQ=255)
                                             tCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCg                                                   <  2:3177522/101‑1 (MQ=255)
                                              ccGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGa                                                  >  1:1433480/1‑101 (MQ=255)
                                               cGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAg                                                 >  2:869590/1‑101 (MQ=255)
                                                gCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAgc                                                <  2:614686/101‑1 (MQ=255)
                                                   ccccGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCa                                             <  1:1761650/101‑1 (MQ=255)
                                                     ccGGGCGGGCAGCGCGTTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAg                                           <  2:3605696/101‑1 (MQ=255)
                                                     ccGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAg                                           >  1:2434248/1‑101 (MQ=255)
                                                        ggcgggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAcc                                        >  2:3878051/1‑101 (MQ=255)
                                                          cgggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAcccc                                      >  1:1269542/1‑101 (MQ=255)
                                                           gggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAccccc                                     >  2:3337799/1‑101 (MQ=255)
                                                           gggcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGAccccc                                     <  2:4603502/101‑1 (MQ=255)
                                                             gcAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGa                                   >  2:3391589/1‑101 (MQ=255)
                                                              cAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGaa                                  >  1:3397875/1‑101 (MQ=255)
                                                                 cgcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgc                               >  2:444980/1‑101 (MQ=255)
                                                                  gcAGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcg                              >  1:852324/1‑101 (MQ=255)
                                                                    aGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCGCGACCAAGGATGACGAGATGGGCGAGACCCACCCGTGGGGCAGGACCCCCGAAAgcgct                            >  2:3010870/1‑99 (MQ=255)
                                                                    aGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggt                            <  2:4726275/101‑1 (MQ=255)
                                                                    aGTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggt                            >  2:2811944/1‑101 (MQ=255)
                                                                     gTTATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtg                           >  2:3818337/1‑101 (MQ=255)
                                                                      ttATGAAATCCTGTCGGTCCTTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtgc                          <  2:2220566/101‑1 (MQ=255)
                                                                      ttATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtgc                          >  2:354090/1‑101 (MQ=255)
                                                                       tATGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtgcg                         >  1:274010/1‑101 (MQ=255)
                                                                         tGAAATCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAgcggtgcggt                       <  1:4620638/101‑1 (MQ=255)
                                                                            aaTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGcc                    <  1:266261/101‑1 (MQ=255)
                                                                            aaTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGcc                    <  1:21649/101‑1 (MQ=255)
                                                                            aaTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGGGcc                    >  1:802852/1‑101 (MQ=255)
                                                                             aTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCa                   <  2:199668/101‑1 (MQ=255)
                                                                             aTCCTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCa                   <  1:4673127/101‑1 (MQ=255)
                                                                               ccTGTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATg                 >  2:190410/1‑101 (MQ=255)
                                                                                cTGTCGGCCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGTCATgg                >  2:4029111/1‑101 (MQ=255)
                                                                                  gTCGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTc              >  1:3643814/1‑101 (MQ=255)
                                                                                    cGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGc            >  2:3455117/1‑101 (MQ=255)
                                                                                    cGGTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGc            >  1:1016203/1‑101 (MQ=255)
                                                                                     ggTCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGcc           <  1:2301488/101‑1 (MQ=255)
                                                                                       tCCGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCTAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCg         <  2:2418742/101‑1 (MQ=255)
                                                                                        ccGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGt        <  2:273976/101‑1 (MQ=255)
                                                                                        ccGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGt        <  2:4478106/101‑1 (MQ=255)
                                                                                        ccGTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGt        <  1:3068462/101‑1 (MQ=255)
                                                                                          gTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGc      >  2:4581676/1‑101 (MQ=255)
                                                                                          gTTTCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGc      >  1:2847578/1‑101 (MQ=255)
                                                                                            taccTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCg    >  2:3800872/3‑101 (MQ=255)
                                                                                             tCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGc   >  2:1823278/1‑101 (MQ=255)
                                                                                             tCCTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGc   >  1:664168/1‑101 (MQ=255)
                                                                                              ccTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCa  <  1:4641942/101‑1 (MQ=255)
                                                                                              ccTGT.AAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCa  <  2:732762/101‑1 (MQ=255)
                                                                                                   |                                                                                                
TGGCCCGCGCGCTGATCGGCAAGGACGAAGGCGACAGCGTCGAGGTCCGCACCCCGGGCGGGCAGCGCAGTTATGAAATCCTGTCGGTCCGTTTCCTGTAAAGCGGGCCCCCGACCAAGGATGACGAGATGGGCGAGCCCCAGTCCGAGCGCAAGACCCCCGAAAGCGGTGCGGTGCCATGGTCGCCCGTGCCGCA  >  NC_014034/549530‑549725

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG