Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 559,029 Δ1 bp 100% intergenic (+78/+29) dcp → / ← fieF peptidyl‑dipeptidase Dcp/ferrous‑iron efflux pump FieF

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034559,0290G→.100.0% 651.8 149intergenic (+78/+29)dcp/fieFpeptidyl‑dipeptidase Dcp/ferrous‑iron efflux pump FieF
Reads supporting (aligned to +/- strand):  new base (118/31):  ref base (0/0):  total (118/31)

Only 100 of 194 total aligned reads displayed.

GCCGCGGCCTTTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGAGGGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGATCGCGCGT  >  NC_014034/558929‑559124
                                                                                                      |                                                                                                
   gcgGCCTTTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCaggaggcc                                                                                               >  1:545943/1‑99 (MQ=255)
    cgGCCTTTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCaggaggccg                                                                                              >  1:2008381/1‑98 (MQ=255)
     ggCCTTTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCaggaggccgg                                                                                             >  2:1006203/1‑97 (MQ=255)
      gCCTGTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAACCCCTGTGCCTTTTTCTTGGGT.AAAATACGCCGGGGGGGG.CCTCCGCAGGA.GGCC.ggg                                                                                          <  2:3039395/101‑1 (MQ=255)
        cTTTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.ggggg                                                                                        >  1:3198547/1‑101 (MQ=255)
         ttttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAACCCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.gggggg                                                                                       <  2:1681764/101‑1 (MQ=255)
         ttttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.gggggg                                                                                       <  2:1481221/101‑1 (MQ=255)
         ttttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.gggggg                                                                                       <  2:1891331/101‑1 (MQ=255)
         ttttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.gggggg                                                                                       >  1:2328412/1‑101 (MQ=255)
          tttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGc                                                                                      >  1:659357/1‑101 (MQ=255)
           ttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCa                                                                                     <  1:2850082/101‑1 (MQ=255)
           ttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCa                                                                                     >  2:2471615/1‑101 (MQ=255)
           ttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCa                                                                                     >  2:3818153/1‑101 (MQ=255)
           ttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCa                                                                                     <  2:727053/101‑1 (MQ=255)
           ttGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCa                                                                                     >  1:100541/1‑101 (MQ=255)
            tGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAg                                                                                    >  2:430564/1‑101 (MQ=255)
             ggAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAgc                                                                                   <  1:1098194/101‑1 (MQ=255)
              gAGGCCTGAGAAAAGTGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAgcg                                                                                  <  2:3156229/101‑1 (MQ=255)
                   cTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGccccc                                                                             <  1:4706223/101‑1 (MQ=255)
                   cTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCAGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGccccc                                                                             <  2:545892/101‑1 (MQ=255)
                    tGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGcccccc                                                                            >  1:3409591/1‑101 (MQ=255)
                     gagaAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCg                                                                           >  1:201440/1‑101 (MQ=255)
                     gagaAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCg                                                                           >  2:39319/1‑101 (MQ=255)
                     gagaAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCg                                                                           >  2:4663281/1‑101 (MQ=255)
                      agaAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGc                                                                          >  1:1215356/1‑101 (MQ=255)
                       gaAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGcc                                                                         >  2:1176802/1‑101 (MQ=255)
                        aaaaGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGccc                                                                        >  2:63916/1‑101 (MQ=255)
                         aaaGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCt                                                                       >  1:1404860/1‑101 (MQ=255)
                         aaaGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCt                                                                       >  2:4541726/1‑101 (MQ=255)
                          aaGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTg                                                                      >  2:579266/1‑101 (MQ=255)
                          aaGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTg                                                                      >  2:2066909/1‑101 (MQ=255)
                             ggCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCt                                                                   >  2:2856557/1‑101 (MQ=255)
                               cGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAg                                                                 >  2:4604264/1‑101 (MQ=255)
                                gTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAgg                                                                >  1:941321/1‑101 (MQ=255)
                                  aTTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCt                                                              >  2:28241/1‑101 (MQ=255)
                                   tttGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCtt                                                             >  1:3555163/1‑101 (MQ=255)
                                      taaccgagAAACCCCTTTTTTTTTTTTTTGGGA.AAAAATCCGCGGGGGGAG.CCCCCGGGGGGGGGCG.GGGGGCCGCCCCCCCCCGCCCTGGCTAGGCTTGc                                                           <  1:3568518/94‑1 (MQ=255)
                                      gAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGcc                                                          >  1:1911623/1‑101 (MQ=255)
                                       aaCCAGGAAACCCTTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCG.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCa                                                         <  2:662588/101‑1 (MQ=39)
                                       aaCCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCa                                                         >  1:2380759/1‑101 (MQ=255)
                                         ccAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGa                                                       >  2:3225387/1‑101 (MQ=255)
                                         ccAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGAGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGa                                                       >  2:1730679/1‑101 (MQ=255)
                                          cAGGAAAACCCGGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGAc                                                      <  2:191194/101‑1 (MQ=255)
                                            ggAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGAGGG.C.GGGGGGCAGCGCCCCCCGCCCTGTCTAGGCTTGCCAGACAt                                                    >  2:1543841/1‑101 (MQ=255)
                                             gAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATc                                                   >  1:1515848/1‑101 (MQ=255)
                                               aaaCCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCa                                                 >  2:4737276/1‑101 (MQ=255)
                                                accccTGTGCTTTTTTTTTGGGT.AAAATCCTCCGGGGGGAG.CCTCCGCAGGA.GGCG.GGGGGGCAGGGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCAt                                                <  1:3164058/99‑1 (MQ=255)
                                                aaCCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCAt                                                >  1:347346/1‑101 (MQ=255)
                                                 aCCCTTTGTTTTTTTTTCGGGG.AAAAAGGGCGGGGGGGGGCCCCCCGGGGGGGGGGG.GGGGGCCGGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCa                                                 <  2:4026736/101‑1 (MQ=255)
                                                 aCCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCAtg                                               >  2:2609136/1‑101 (MQ=255)
                                                  cccTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCAtgt                                              >  2:2345139/1‑101 (MQ=255)
                                                  cccTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCAtgt                                              <  2:755149/101‑1 (MQ=255)
                                                   ccTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCAtgtg                                             >  1:2449206/1‑101 (MQ=255)
                                                     tgtgTTTTTTTCTGGGGT.AAAATCCGCGGGGGGGAG.CCTCCGCAGGA.GGCG.GGGGGGCAGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGga                                           <  1:2259414/101‑1 (MQ=255)
                                                     tgtgCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGga                                           >  1:3739313/1‑101 (MQ=255)
                                                      gtttttttttTTTGGGA.AAAATCCG.GGGGGGGGG.CCCCCCGGGGGGGGCG.GGGGGGCGGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGgat                                          <  1:3818388/97‑1 (MQ=255)
                                                        gCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGgatga                                        >  2:4490099/1‑101 (MQ=255)
                                                        gCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGgatga                                        >  1:525902/1‑101 (MQ=255)
                                                         tttttttTTTGGGG.AAAA.ACGGGGGGGGGGG.CGCCCCCGGGGGGGGGGGGGGGGCCGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGgatga                                        <  1:1579893/100‑1 (MQ=255)
                                                         cTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGgatgat                                       >  1:3257166/1‑101 (MQ=255)
                                                         cTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGgatgat                                       >  1:3322790/1‑101 (MQ=255)
                                                          ttttttCTTGGGT.AAAATACGCGGGGGGGAG.CCGCCGCAGGA.GGCG.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATc                                      <  1:2271762/101‑1 (MQ=255)
                                                           tttttTTGGGGA.AAAATCGGGGGGGGGGGG.CCCCCGCGGGA.GGCG.GGGGGGCGGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCa                                     <  1:2722105/101‑1 (MQ=255)
                                                            ttttTTTGGGTAAAAAGCCGCGGGGGGGCC.CCCCCGC.GGGGGGCG.GGGGGGCGCCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCa                                     <  2:162193/101‑1 (MQ=255)
                                                            ttttGTTGGTG.AAAAGGCGGCGGGGGGGG.CCCCCGC.GGGGGGGG.GGGGGGCGGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATcga                                    <  2:2942329/101‑3 (MQ=255)
                                                            ttttCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCAc                                    >  1:590044/1‑101 (MQ=255)
                                                            ttttCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCAc                                    <  2:1197979/101‑1 (MQ=255)
                                                              ttCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGt                                  >  1:3968744/1‑101 (MQ=255)
                                                                cTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCg                                >  2:1147446/1‑101 (MQ=255)
                                                                cTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCg                                >  2:4695272/1‑101 (MQ=255)
                                                                 ttGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCgg                               >  2:1071024/1‑101 (MQ=255)
                                                                 ttGGGT.AAAAGCCGCCGGGGGGAC.CCCCCGCAGGA.GGCG.GGGGGGCGGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCgg                               <  2:1335438/101‑1 (MQ=255)
                                                                  tGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGt                              >  2:233115/1‑101 (MQ=255)
                                                                  tGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGt                              >  2:2981834/1‑101 (MQ=255)
                                                                   gggT.AAAATACGGCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTc                             >  1:2566714/1‑101 (MQ=255)
                                                                    ggT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGCTGATCACGTCGGTCt                            >  2:3696478/1‑101 (MQ=255)
                                                                     gT.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTg                           >  1:1874203/1‑101 (MQ=255)
                                                                      t.AAAATACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGc                          >  2:2284487/1‑101 (MQ=255)
                                                                        aaaaTACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCg                         >  1:2503595/1‑101 (MQ=255)
                                                                         aaaTACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCCGACATCCATGTGGATGATCACGTCGGTCTGCgg                        >  1:4105330/1‑101 (MQ=255)
                                                                          aaTACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGa                       >  1:3096550/1‑101 (MQ=255)
                                                                            tACGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGCGGATGATCACGTCGGTCTGCGGGTa                     >  1:3686285/1‑101 (MQ=255)
                                                                             aCGCGGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAg                    <  1:3878389/101‑1 (MQ=255)
                                                                             aCGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAg                    >  2:3562722/1‑101 (MQ=255)
                                                                             aCGCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAg                    >  1:2884982/1‑101 (MQ=255)
                                                                             aCGCCGGGGGGAG.ACTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCAAGGCTTGCCAGACATCCATGTGGCAGAGCACGTCGGTCTGCCGATAg                    >  1:4552123/1‑101 (MQ=255)
                                                                               gggggggggCC.CCCCCGC.GGGGGGCG.GGGGGGCAGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGc                  <  2:160846/98‑1 (MQ=255)
                                                                               gCCGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGc                  >  2:3183873/1‑101 (MQ=255)
                                                                                 cGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCt                >  2:3489194/1‑101 (MQ=255)
                                                                                 cGGGGGGAG.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCt                >  1:1692315/1‑101 (MQ=255)
                                                                                  ggggggTG.CCTCCGGAGGAGGG.C.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTc               <  1:771282/101‑1 (MQ=255)
                                                                                      gggg.CCCCCGGAGGGGGGCG.GGGGGGCAGCCCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTcgcg            <  1:2759492/98‑1 (MQ=255)
                                                                                        gg.CCTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGAt         <  2:1425026/100‑1 (MQ=255)
                                                                                         g.CCCCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGATc        <  2:2958636/101‑1 (MQ=255)
                                                                                           ccTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGATcg       <  2:2323321/101‑1 (MQ=255)
                                                                                           ccTCCGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAAGCCCCGCGGATcg       >  1:1778240/1‑101 (MQ=255)
                                                                                             cccGCAGGA.GGCC.GGGTGTCATCGCCCCCCTCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGATcgcg     <  2:819553/100‑1 (MQ=255)
                                                                                              ccGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTTGCGGATcgccg    >  1:4418104/1‑99 (MQ=255)
                                                                                              ccGCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCCCGGGTcccgg    >  2:3433167/1‑100 (MQ=255)
                                                                                                gCAGGA.GGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGATCGCGCGt  >  2:1247868/1‑101 (MQ=255)
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GCCGCGGCCTTTTGGAGGCCTGAGAAAAGGGCGTATTTGAACCAGGAAAACCCTGTGCTTTTTTCTTGGGT.AAAATACGCCGGGGGGAG.CCTCCGCAGGAGGGCC.GGGGGGCAGCGCCCCCCGCCCTGGCTAGGCTTGCCAGACATCCATGTGGATGATCACGTCGGTCTGCGGATAGGCCTCGCGGATCGCGCGT  >  NC_014034/558929‑559124

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG