Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,802 Δ1 bp 7.8% intergenic (‑98/+68) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8020C→. 7.8% 31.4 ‑1.5 ‑0.2154intergenic (‑98/+68)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (9/3):  ref base (78/64):  total (87/67)
Fisher's exact test strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.78e-01

Only 100 of 224 total aligned reads displayed.

CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902
                                                                                                     |                                                                                                    
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gc                                                                                                      >  1:2450651/1‑101 (MQ=255)
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gc                                                                                                      >  1:90891/1‑101 (MQ=255)
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gc                                                                                                      <  2:252795/101‑1 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcc                                                                                                     <  2:2681871/101‑1 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcc                                                                                                     >  2:4391805/1‑101 (MQ=255)
    ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gccccc                                                                                                  <  2:3447984/101‑1 (MQ=255)
    ccTGCCTCCAGTTTCTGTAGGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gccccc                                                                                                  <  2:4601081/101‑1 (MQ=255)
     cTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccc                                                                                                 <  2:197785/101‑1 (MQ=255)
     cTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccc                                                                                                 <  1:238397/101‑1 (MQ=255)
     cTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccc                                                                                                 <  1:550468/101‑1 (MQ=255)
      tGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gccccccc                                                                                                >  2:842877/1‑101 (MQ=255)
       gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccccc                                                                                               <  1:676528/101‑1 (MQ=255)
       gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccccc                                                                                               <  2:4136954/101‑1 (MQ=255)
        ccTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gccccccccc                                                                                              <  1:2736121/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccccccc                                                                                             <  1:2932814/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGGGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGGCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccccccc                                                                                             <  1:1369223/101‑1 (MQ=255)
          tCCAGTTTCTGTATGTGAACCGGGGCTTACCCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCg                                                                                            <  2:3111338/101‑1 (MQ=255)
          cccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCCGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCg                                                                                            <  2:3799883/100‑1 (MQ=255)
           ccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gcccccccccgac                                                                                           <  2:2462008/101‑4 (MQ=255)
            cAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGAc                                                                                          <  1:1162457/101‑1 (MQ=255)
              gTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.Gccccccccccccccg                                                                                        >  1:663538/1‑96 (MQ=255)
              gTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACgg                                                                                        >  2:1210595/1‑101 (MQ=255)
               tttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACggg                                                                                       >  2:697651/1‑101 (MQ=255)
                ttCTGTATTGGAGCCGGGGCTTTAGCCTGTGGCGGCGGTGGGGCAATGGCCGGCCGGGGCTTGTGAAGGCGCGGCGGCATCCT.GCCCCCCCCCCGACGGGa                                                                                      <  2:2571412/101‑1 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGCCgggg                                                                                      >  1:1549373/1‑100 (MQ=255)
                 tCTGTATGGGAACCGGGGCTTACGCCTGGGGCTGGGGTGGGGCAATGCCCCGCAGGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGag                                                                                     <  1:1237176/101‑1 (MQ=255)
                    ggaggtgAACCGGGGCTTACGCCGGGGGCGGCGGTGGGGGAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCC.GCCCCCCCCCCGACGGGagggc                                                                                  <  1:76348/97‑1 (MQ=255)
                         tgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGCCGGGggggcggggc                                                                             >  2:275452/1‑101 (MQ=255)
                         tgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCCCCGGGggggcagggc                                                                             >  2:2789451/1‑101 (MQ=255)
                           aaCCGGGGCTTTCGCCTGGGGGTGCGGTGGGGCAATGACCCGCCCGGGGTTGTGAAGGCCCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTg                                                                           <  1:1332713/101‑1 (MQ=255)
                              cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGtt                                                                        <  1:520710/101‑1 (MQ=255)
                              cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.G.CCCCCCCCCGACGGGAGGGCAGGGCTGGttt                                                                       >  2:430220/1‑101 (MQ=255)
                               ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.G.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTc                                                                      >  1:1958995/1‑101 (MQ=255)
                                gggCTTACGCCTGGGGGTGCGGTGGGGCAATGCCCCGCGCGGGCTTGGAAAGGCGCTGGGGCATCCT.CCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTc                                                                      <  1:38498/101‑1 (MQ=255)
                                 ggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCg                                                                     >  1:310143/1‑101 (MQ=255)
                                  gCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCCACGGGGGGGCGGGGCCGGTTTCgg                                                                    >  1:2973921/1‑101 (MQ=255)
                                   cTTGCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACCGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCggg                                                                   <  2:3919278/101‑1 (MQ=255)
                                     tACGCCTGTGGCTGCGGTGGGGCAATGACCCGCAGGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcg                                                                 <  2:3177069/101‑1 (MQ=255)
                                        gCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGgggggga                                                              >  2:4159378/1‑99 (MQ=255)
                                        gCCTGGGGCTGGGGGGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                              <  1:4193062/101‑1 (MQ=255)
                                         ccTGTGGCTGCGGTGGGGCAATGACCCGAACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCaa                                                             <  1:610502/101‑1 (MQ=255)
                                         ccTGTGGCTGCGGGGGGGCAATGGCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCaa                                                             <  2:613866/101‑1 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAg                                                            >  1:1992129/1‑101 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAg                                                            <  2:3286231/101‑1 (MQ=255)
                                          cTGGGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAg                                                            <  2:1758041/101‑1 (MQ=255)
                                           tgtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCCACGGGAGGGGAGGGCTGGATTCGGGCGGCCAgg                                                           >  1:1037403/1‑101 (MQ=255)
                                              ggCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTCGGGCGGCAAGgcgc                                                        >  2:2891015/1‑101 (MQ=255)
                                              ggCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGGTTCGGGCGGCAAAgcgc                                                        >  2:2217473/1‑101 (MQ=255)
                                                cTGGGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                      <  1:1516839/101‑1 (MQ=255)
                                                  gggggggggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                    <  2:4480546/96‑1 (MQ=255)
                                                  gCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTTGGGGGGAGCGTCGCCcgc                                                    >  2:1452520/1‑101 (MQ=255)
                                                  gCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                    <  1:4640006/101‑1 (MQ=255)
                                                  gCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGGTTCGGGCGGCACGGGGCCcgg                                                    >  2:5591/1‑100 (MQ=255)
                                                      tGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCcgcggcg                                                >  1:1816421/1‑101 (MQ=255)
                                                       ggggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.G.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcg                                              >  2:3224584/1‑101 (MQ=255)
                                                         ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                             >  2:1243657/1‑101 (MQ=255)
                                                           cAATGGCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           <  1:4528453/101‑1 (MQ=255)
                                                           cAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           >  1:2607404/1‑101 (MQ=255)
                                                           cAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                           <  1:3492182/101‑1 (MQ=255)
                                                            aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCg                                          >  2:4059811/1‑101 (MQ=255)
                                                             aTGCCCCCCCCGGGCTTGGGAAGGCGCTGCGGCATCCC.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGt                                         <  1:4141488/101‑1 (MQ=255)
                                                              tggcccccccGGGCTTGGTGAGGGGCTTCCGCCTCCTGGCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGt                                         <  2:1549947/92‑1 (MQ=255)
                                                              tGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTg                                        <  2:408667/101‑1 (MQ=255)
                                                              tGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGCCCGtc                                        >  1:3148991/1‑100 (MQ=255)
                                                               gACCCGCACGGGCTTGTGAAGGCTCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGc                                       <  2:2149456/101‑1 (MQ=255)
                                                                 cccGCGCGGGCTTGTGAAGGCGCTGCGGCACCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                     <  2:1099108/101‑1 (MQ=255)
                                                                   cGCCCGGGTTTGTGAATGCGCTGCTGCACCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGc                                   <  1:1911834/101‑1 (MQ=255)
                                                                   cGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGc                                   <  2:1653474/101‑1 (MQ=255)
                                                                   cGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCCGc                                   >  1:2054731/1‑101 (MQ=255)
                                                                     cACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGCCGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCCGGCCTGCtt                                 >  2:2439241/1‑101 (MQ=255)
                                                                     cACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                 <  2:4448541/101‑1 (MQ=255)
                                                                      ccGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                                <  2:2974114/100‑1 (MQ=255)
                                                                      ccGGGCTTGGGAAGGCGCTGCGGCCTCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                                <  2:1801889/100‑1 (MQ=255)
                                                                      aCGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTTGGGCGGCAAGGCGCCCCCGGGGCCGCCGTGCCTGCTTa                                >  1:3388125/1‑101 (MQ=255)
                                                                       cGGGCTTGTGAAGGCGCTGCGGCCTCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTAc                               <  2:3558645/101‑1 (MQ=255)
                                                                       cGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCTCCTCGACGGGAGGGCAGGGCTGCTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTAc                               <  1:4060053/101‑1 (MQ=255)
                                                                        gggCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACg                              <  1:3966871/101‑1 (MQ=255)
                                                                         ggCTTGTGAAGGCGCTTCCGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGc                             <  1:3085056/101‑1 (MQ=255)
                                                                           cTTGGGAGGGCCCTGCGGCATCCG.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCt                           <  1:5596/101‑1 (MQ=255)
                                                                             tgtgAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGc                         >  1:2196275/1‑101 (MQ=255)
                                                                             tgtgAACGCCCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                         <  2:4333624/101‑1 (MQ=255)
                                                                              gtgAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                        <  2:4203742/101‑1 (MQ=255)
                                                                              gggAAGGCGCTGCGGCATCCT.CCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                        <  1:1701773/99‑1 (MQ=255)
                                                                               tgAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAg                       >  2:2307294/1‑101 (MQ=255)
                                                                                gAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGc                      <  1:4359525/101‑1 (MQ=255)
                                                                                gAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGc                      <  1:2860586/101‑1 (MQ=255)
                                                                                  aGGCGCTGCGGCTTCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCgg                    <  1:2217597/101‑1 (MQ=255)
                                                                                  aGGCGCTGCGGCATCCT.G.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGc                   <  1:3217109/101‑1 (MQ=255)
                                                                                     gccggCGGCCTCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                 <  1:442364/97‑1 (MQ=255)
                                                                                     cgcTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                 <  1:2596189/101‑1 (MQ=255)
                                                                                     cgcTGCGGCATCCT.G.CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCgg                <  1:430264/101‑1 (MQ=255)
                                                                                      gcTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCGGGGCTGGTTTCCGGCCGCAAGGCGCCCCCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCgg                >  1:2703830/1‑101 (MQ=255)
                                                                                      gcTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCgg                <  1:359962/101‑1 (MQ=255)
                                                                                       cTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCCGCAGCGGCCGGa               >  2:1375812/1‑101 (MQ=255)
                                                                                        tGCGGCATCCT.GCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGGCGGGCCTGCTTTCCCCTGCAGCGGCCgggc              >  2:2497923/1‑99 (MQ=255)
                                                                                          cGGCATCCT.GCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCCGCTTACGCCTGCCGCGGCCgggacg            >  2:3539597/1‑97 (MQ=255)
                                                                                           ggCATCCT.GCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGGGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGACCga           >  1:2600684/1‑101 (MQ=255)
                                                                                              ctCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt        <  2:4131790/100‑1 (MQ=255)
                                                                                               tCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAtt       <  2:481282/101‑1 (MQ=255)
                                                                                                    gCCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGc   >  2:733131/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCT.GCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG