Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 71,452 Δ1 bp 100% coding (42/591 nt) gidB ← methyltransferase GidB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403471,4520T→.100.0% 1056.0 237coding (42/591 nt)gidBmethyltransferase GidB
Reads supporting (aligned to +/- strand):  new base (124/113):  ref base (0/0):  total (124/113)

Only 100 of 267 total aligned reads displayed.

GCAGAATCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCTTTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTGGT  >  NC_014034/71352‑71547
                                                                                                    |                                                                                               
gCAGAATCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCt                                                                                                 >  1:1966928/1‑101 (MQ=255)
 cAGAATCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTACACTTTTTCAGCAACGCtt                                                                                                >  2:2698071/1‑101 (MQ=255)
  aGAATCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCttc                                                                                               <  2:3971787/101‑2 (MQ=255)
  aGAATCTGAGCCCAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCttc                                                                                               <  2:424510/101‑2 (MQ=255)
   gAATCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCttcc                                                                                              >  1:3833374/1‑99 (MQ=255)
    aaTCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCttcca                                                                                             >  2:4582118/1‑98 (MQ=255)
    aaTCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCttcca                                                                                             >  1:3246861/1‑98 (MQ=255)
     aTCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCttccag                                                                                            <  2:3230173/101‑5 (MQ=255)
      tCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGc                                                                                          <  1:3344665/101‑1 (MQ=255)
      tCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGc                                                                                          >  1:841918/1‑101 (MQ=255)
        tGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCg                                                                                        >  1:3324386/1‑101 (MQ=255)
         gAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGc                                                                                       >  2:502693/1‑101 (MQ=255)
         gAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGc                                                                                       >  2:2258757/1‑101 (MQ=255)
           gCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTc                                                                                     <  2:4477351/101‑1 (MQ=255)
           gCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTc                                                                                     <  2:881340/101‑1 (MQ=255)
            ccGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCg                                                                                    <  2:3583052/101‑1 (MQ=255)
              gAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAg                                                                                  <  1:3897499/101‑1 (MQ=255)
              gAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAg                                                                                  >  2:73484/1‑101 (MQ=255)
              gAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCAACTTTTTCAGCAACGC.TTCCAGCCGCTCGAg                                                                                  >  1:4399206/1‑101 (MQ=255)
                aTCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGC.GAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCCAGGCg                                                                               >  2:4111834/1‑101 (MQ=255)
                    aGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTg                                                                            <  1:2008151/101‑1 (MQ=255)
                     gAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGa                                                                           >  1:1474826/1‑101 (MQ=255)
                      aaaaTGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGaa                                                                          <  1:4369827/101‑1 (MQ=255)
                       aaaTGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAAt                                                                         <  1:1667341/101‑1 (MQ=255)
                        aaTGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAAtt                                                                        <  1:4674618/101‑1 (MQ=255)
                             cgcgTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTc                                                                   <  1:3740211/101‑1 (MQ=255)
                                gTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACAC.TTCCAGCCGCACGCGGCGGGGGATTGTTTCACg                                                                >  2:1100628/1‑101 (MQ=255)
                                 tCCAGCCTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGt                                                               <  2:1699129/101‑1 (MQ=255)
                                   cAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGa                                                             <  2:3527477/101‑1 (MQ=255)
                                     gACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGaaa                                                           >  2:2274400/1‑101 (MQ=255)
                                     gACGTCCACAAGCGGCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGaaa                                                           <  1:2170926/101‑1 (MQ=255)
                                      aCTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCATGTGAAAc                                                          <  2:3393224/101‑1 (MQ=255)
                                      aCTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAAc                                                          <  1:4652442/101‑1 (MQ=255)
                                       cTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACa                                                         >  2:2824707/1‑101 (MQ=255)
                                        ttCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACAt                                                        <  2:1927190/101‑1 (MQ=255)
                                        ttCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACAt                                                        >  2:263164/1‑101 (MQ=255)
                                          ccACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCg                                                      >  2:709665/1‑101 (MQ=255)
                                          ccACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCg                                                      >  1:2622711/1‑101 (MQ=255)
                                          ccACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCg                                                      <  2:1753902/101‑1 (MQ=255)
                                            acaAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAc                                                    >  1:4536924/1‑101 (MQ=255)
                                            acaAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAc                                                    <  1:298002/101‑1 (MQ=255)
                                            acaAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAc                                                    >  2:2080178/1‑101 (MQ=255)
                                             caAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAcc                                                   <  2:2877106/101‑1 (MQ=255)
                                             caAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAcc                                                   <  1:3960461/101‑1 (MQ=255)
                                             caAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAcc                                                   <  1:1129810/101‑1 (MQ=255)
                                             caAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGAcc                                                   <  1:3576059/101‑1 (MQ=255)
                                                gCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGt                                                <  1:502744/101‑1 (MQ=255)
                                                gCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGt                                                <  1:790080/101‑1 (MQ=255)
                                                 cGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGtt                                               >  1:1067344/1‑101 (MQ=255)
                                                    ccGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTg                                            <  1:1464301/101‑1 (MQ=255)
                                                      gcgGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCg                                          >  2:3126956/1‑101 (MQ=255)
                                                       cgGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGa                                         >  2:3738726/1‑101 (MQ=255)
                                                       cgGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGa                                         <  1:2994826/101‑1 (MQ=255)
                                                        ggAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGaa                                        <  2:2370848/101‑1 (MQ=255)
                                                         gagagaCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGaaa                                       <  2:4664598/101‑1 (MQ=255)
                                                         gagagaCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGaaa                                       <  1:4025618/101‑1 (MQ=255)
                                                         gagagaCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGCGAAAAATCGACCAGGTTTGCGaaa                                       >  2:4357694/1‑101 (MQ=255)
                                                            ataCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCt                                    >  2:827327/3‑101 (MQ=255)
                                                             gaCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCtt                                   >  1:282387/1‑101 (MQ=255)
                                                             gaCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATGGTTTCACGGGAACAACCAACCAGTTTTGCGAAAGCtt                                   >  2:1310507/1‑101 (MQ=255)
                                                              cgatctttGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTc                                  <  2:4610022/95‑1 (MQ=255)
                                                              aCCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTc                                  >  2:2593533/1‑101 (MQ=255)
                                                               ccAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTcc                                 >  1:2908094/1‑101 (MQ=255)
                                                               ccAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTcc                                 <  2:2765612/101‑1 (MQ=255)
                                                                gaggttGGTGGCCGGATCCCACTTTTTCAGCGACGC.TGCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCg                                <  2:1354798/97‑1 (MQ=255)
                                                                cAGATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCg                                >  2:4381793/1‑101 (MQ=255)
                                                                 aGATTGATGGCCGGATTCCCCTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGa                               <  1:4579026/101‑1 (MQ=255)
                                                                  gATTGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGAc                              <  1:3917651/101‑1 (MQ=255)
                                                                    ttGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTc                            >  1:2412872/1‑101 (MQ=255)
                                                                    ttGATGGCCGGATTCCACTTTTTCAGCAACGC.TCCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTc                            <  1:2305155/101‑1 (MQ=255)
                                                                     tGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCg                           <  1:998606/101‑1 (MQ=255)
                                                                     tGATGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCCAAAGCTCCCGACTCg                           >  1:1910288/1‑101 (MQ=255)
                                                                       aTGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCCGTTTTGTGAAAGCTTCCGACTCggc                         >  2:1041448/1‑100 (MQ=255)
                                                                       aTGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGt                         >  1:1939367/1‑101 (MQ=255)
                                                                       aTGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGt                         >  1:2151004/1‑101 (MQ=255)
                                                                        tGGCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTc                        <  2:4175769/101‑1 (MQ=255)
                                                                         ggCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCa                       >  1:1573346/1‑101 (MQ=255)
                                                                          gCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCAt                      <  2:3844497/101‑1 (MQ=255)
                                                                          gCCGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCAt                      <  1:4262754/101‑1 (MQ=255)
                                                                           ccGGATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATg                     >  2:1913908/1‑101 (MQ=255)
                                                                             ggATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTc                   >  1:2503978/1‑101 (MQ=255)
                                                                              gATTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTcc                  >  1:1595527/1‑101 (MQ=255)
                                                                               aTTCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCg                 >  2:4543850/1‑101 (MQ=255)
                                                                                ttCCACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGc                >  2:3269642/1‑101 (MQ=255)
                                                                                  ccACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGccc              <  2:775527/101‑1 (MQ=255)
                                                                                   cACTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCg             <  1:120664/101‑1 (MQ=255)
                                                                                    aCTTTTTCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCgg            >  2:3690292/1‑101 (MQ=255)
                                                                                        tttCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCt        >  2:2726057/1‑101 (MQ=255)
                                                                                        tttCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCt        >  1:921782/1‑101 (MQ=255)
                                                                                        tttCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGGCATGTCCGCCCGGCCCt        >  2:4104084/1‑101 (MQ=255)
                                                                                         ttCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCtt       >  1:349903/1‑101 (MQ=255)
                                                                                         ttCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCtt       >  1:4193995/1‑101 (MQ=255)
                                                                                         ttCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCtt       >  2:2998955/1‑101 (MQ=255)
                                                                                          tCAGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCttt      >  1:2268849/1‑101 (MQ=255)
                                                                                            aGCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTg    >  1:4498621/1‑101 (MQ=255)
                                                                                             gCAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTgg   <  2:1502506/101‑1 (MQ=255)
                                                                                              cAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTGGt  >  2:4428508/1‑101 (MQ=255)
                                                                                              cAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTGGt  >  2:589347/1‑101 (MQ=255)
                                                                                              cAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTGGt  <  1:644290/101‑1 (MQ=255)
                                                                                              cAACGC.TTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTGGt  >  2:1857926/1‑101 (MQ=255)
                                                                                                    |                                                                                               
GCAGAATCTGAGCCGAATCCAGAAAATGACGCGTCCAGACTTCCACAAGCGTCCGCGGAGAGACCAGATTGATGGCCGGATTCCACTTTTTCAGCAACGCTTTCCAGCCGCTCGAGGCGTTGAATTGTTTCACGTGAAACATCGACCAGTTTTGCGAAAGCTTCCGACTCGGTCATGTCCGCCCGGCCCTTTTGGT  >  NC_014034/71352‑71547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG