Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 904,926 Δ1 bp 100% intergenic (+30/‑166) RCAP_rcc00821 → / → murB hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034904,9260T→.100.0% 480.4 158intergenic (+30/‑166)RCAP_rcc00821/murBhypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (83/73):  ref base (0/0):  total (85/73)

Only 100 of 183 total aligned reads displayed.

TGGCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.GCT..G..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTG  >  NC_014034/904826‑905019
                                                                                                    |                                                                                                    
tGGCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCctct                                                                                                      <  1:1835853/101‑1 (MQ=255)
 ggCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGCTGCCCCCCCCtt                                                                                                     >  2:2759874/1‑101 (MQ=255)
  gCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGCGGCCCCCCCCttt                                                                                                    >  2:843619/1‑101 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                   <  2:1758115/101‑1 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                   <  1:496261/101‑1 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                   <  2:685912/101‑1 (MQ=255)
   cAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCtttt                                                                                                   <  1:1693987/101‑1 (MQ=255)
    aGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCttttg                                                                                                  <  2:1273914/101‑2 (MQ=255)
          gCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cg                                                                                      <  2:2964065/101‑2 (MQ=255)
            tGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgcc                                                                                    <  1:1186027/101‑4 (MQ=255)
             gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgcca                                                                                   <  2:3476220/101‑5 (MQ=255)
             gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgcca                                                                                   <  2:3171964/101‑5 (MQ=255)
              ggCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgccaa                                                                                  <  1:4262742/100‑6 (MQ=255)
              ggCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgccaa                                                                                  <  2:4382424/100‑6 (MQ=255)
              aGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgccaa                                                                                  <  2:117406/101‑6 (MQ=255)
               gCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..G..cgccaaa                                                                                 <  2:363125/101‑7 (MQ=255)
                   gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTc                                                                             <  1:3916984/101‑1 (MQ=25)
                   gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCCC.TTTTG.GCTGCG..C..CAAAATTc                                                                             <  1:2689987/101‑1 (MQ=25)
                   gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGAGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTc                                                                             <  1:4606020/101‑1 (MQ=25)
                    ctgcGCTGCCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCa                                                                            <  2:4197610/101‑1 (MQ=25)
                      gcgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCAcc                                                                          <  2:2689324/101‑1 (MQ=25)
                       cgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGGCTGCCCCCCTC.TTTTG.GCTGCG..C..CACAATTCAccc                                                                         >  1:2200259/1‑101 (MQ=25)
                         cTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGGACTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTTAccccc                                                                       >  1:4007642/1‑101 (MQ=21)
                         cTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCAccccc                                                                       <  1:3791527/101‑1 (MQ=25)
                         cTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCAccccc                                                                       <  1:1151868/101‑1 (MQ=25)
                          tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCAcccccc                                                                      <  2:2433260/101‑1 (MQ=21)
                          tGGCCGGGGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCAcccccc                                                                      <  1:2250421/101‑1 (MQ=21)
                              cGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCg                                                                  <  2:216038/101‑1 (MQ=21)
                              cGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCg                                                                  >  2:1562066/1‑101 (MQ=21)
                              cGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCg                                                                  >  1:3585153/1‑101 (MQ=21)
                              cGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCACTGGCCCCCTATTTTGG.GTTC.G..C..CAAAATTCACCCCCCGGCg                                                                  >  2:510405/1‑101 (MQ=21)
                               ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGt                                                                 >  1:2843892/1‑101 (MQ=21)
                               ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCC.CCTTTTG.GCTGCG..C..CAAAATTCCCCCCCCGGCGt                                                                 >  2:4046564/1‑101 (MQ=21)
                                 tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CCAAATTTACCCCCCGGCGCAt                                                               >  2:2244705/1‑101 (MQ=21)
                                 tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGGAt                                                               >  2:1288684/1‑101 (MQ=21)
                                 tGCGGCGGCGCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTAt                                                               <  2:286612/101‑1 (MQ=21)
                                  gcggcgGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGATGGCCCCCTCTTTTTGCTGC..G..C..CAAAATTCCCCCCCCGGCGTGtt                                                              >  1:4478073/1‑101 (MQ=255)
                                   cggccgCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCCGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTAttt                                                             <  1:1269193/101‑1 (MQ=18)
                                    ggcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTg                                                            <  2:226556/101‑1 (MQ=21)
                                       ggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCAc                                                         >  1:3087841/1‑101 (MQ=21)
                                        gcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCAcc                                                        <  2:4154842/101‑1 (MQ=21)
                                         ctctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCa                                                       >  2:261168/1‑101 (MQ=21)
                                         ctctTTGCCGCGATGCACCGGCGCGACGGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCa                                                       <  2:723162/101‑1 (MQ=18)
                                          tctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCCCCAg                                                      >  1:1764819/1‑101 (MQ=18)
                                          tctTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAg                                                      >  1:947129/1‑101 (MQ=21)
                                           ctTTGCCGCGATGCACGGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTG.CCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                    <  2:345015/101‑1 (MQ=18)
                                            tttGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                    <  2:2594163/101‑1 (MQ=21)
                                              tGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGaaa                                                  <  1:4295321/101‑1 (MQ=21)
                                               gCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCCGCCGATTTGCACCCGGgaaa                                                 >  2:1643011/1‑101 (MQ=17)
                                                 cgcgATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAg                                               <  2:4463484/101‑1 (MQ=21)
                                                   cgATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGcc                                             >  2:3704511/1‑101 (MQ=21)
                                                    gATGCACCTGCGTGACTGAGGGTTGGGCGGGGGGGCGCTGTCGCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCACGAAAAAGccc                                            <  2:1893506/101‑1 (MQ=12)
                                                     aTGCAGCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                           <  1:3719299/101‑1 (MQ=18)
                                                     aTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                           >  2:1903372/1‑101 (MQ=21)
                                                      tGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCgg                                          >  2:3252788/1‑101 (MQ=21)
                                                       gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGc                                         >  2:70581/1‑101 (MQ=21)
                                                       gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGc                                         >  1:91222/1‑101 (MQ=21)
                                                       gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGCATCTGCACCAGGGAAAAGCCCGGc                                         >  1:4183811/1‑101 (MQ=18)
                                                         aCCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGt                                       >  2:2860411/1‑101 (MQ=21)
                                                           cGGCGCGGCTGAGCGCGGGGCAGGGGGGCGCTGCCCCCCTC.TGTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                     <  2:1562359/101‑1 (MQ=18)
                                                           cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                     <  1:535047/101‑1 (MQ=21)
                                                            ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                    >  2:917811/1‑101 (MQ=21)
                                                             gcgcGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttttt                                   <  2:3744865/101‑1 (MQ=21)
                                                              cgcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTc                                  <  2:4144997/101‑1 (MQ=21)
                                                               gcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttttgc                                 <  1:3081985/101‑3 (MQ=21)
                                                                 gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                               >  2:3773198/1‑101 (MQ=21)
                                                                 gACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTg                               >  2:2307710/1‑101 (MQ=21)
                                                                   cTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGt                             >  2:3225510/1‑101 (MQ=21)
                                                                   cTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGt                             >  1:1026588/1‑101 (MQ=21)
                                                                     gAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTCGTcc                           >  1:2097377/1‑100 (MQ=18)
                                                                      aGGGCGTGGCAGGGGGGTGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGa                          <  2:548403/101‑1 (MQ=21)
                                                                          cGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                      >  2:382991/1‑101 (MQ=21)
                                                                           ggggCAGGGGGGGGCTGCTCTCCTC.TTTTG.GCTGCG..C..CAAAATTCGCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATAc                     <  1:2401749/101‑1 (MQ=25)
                                                                            gggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                    <  1:1862578/101‑1 (MQ=21)
                                                                            gggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                    >  2:3149875/1‑101 (MQ=21)
                                                                             ggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGc                   >  2:1394614/1‑101 (MQ=21)
                                                                               cAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTTGTCGAAATACGCCg                 >  1:1607611/1‑101 (MQ=21)
                                                                               cAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCg                 <  1:4433644/101‑1 (MQ=21)
                                                                                aGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                >  2:1679965/1‑101 (MQ=25)
                                                                                aGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                >  1:2445052/1‑101 (MQ=25)
                                                                                aGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                >  1:2754827/1‑101 (MQ=25)
                                                                                 ggggggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCggg               >  1:2477493/1‑101 (MQ=25)
                                                                                 ggggggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACCCCggg               >  1:996881/1‑101 (MQ=21)
                                                                                  gggggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGCAAAAGCCCGGCGCTTTTCCTGGTCGAAATACGCCgggg              >  1:1286980/1‑101 (MQ=21)
                                                                                  gggggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggg              >  1:3276711/1‑101 (MQ=25)
                                                                                   ggggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGACCGGCGTTTTTCCTAGTCGAAATACGCCggggg             >  1:3893903/1‑101 (MQ=21)
                                                                                    gggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGTGTTTTTCCTGGTCGAAATACGCCgggggg            <  2:3904161/101‑1 (MQ=25)
                                                                                    gggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg            <  1:1327064/101‑1 (MQ=25)
                                                                                    gggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg            <  1:3526139/101‑1 (MQ=25)
                                                                                     ggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGa           >  1:1014990/1‑101 (MQ=25)
                                                                                     ggCGCTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGCTTTTCCTCGTCGAAATACGCCGGAGGGa           >  1:649687/1‑101 (MQ=21)
                                                                                     cgatcTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGa           <  1:3450892/97‑1 (MQ=25)
                                                                                       cgcTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGt         <  2:1468955/101‑1 (MQ=25)
                                                                                        gcTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc        >  2:4524558/1‑101 (MQ=37)
                                                                                         cTGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc       <  2:3584934/101‑1 (MQ=37)
                                                                                         cTGCCCCCCTC.TTTTG.GCT..GCAC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc       <  1:3009041/101‑1 (MQ=37)
                                                                                          tGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg      <  1:4550607/101‑1 (MQ=37)
                                                                                          tGCCCCCCTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg      <  1:1405022/101‑1 (MQ=37)
                                                                                             cccccTC.TTTTG.GCT..G..CGACAAAATCCACCCCCCGGCGTATTTGCACCAGGAAAAACCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCtt   <  2:1796961/101‑1 (MQ=21)
                                                                                              ccccTC.TTTTG.GCTGCG..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTg  >  2:2020917/1‑101 (MQ=37)
                                                                                                    |                                                                                                    
TGGCAGGCGGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.GCT..G..C..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTG  >  NC_014034/904826‑905019

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG