Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,200,794 +G 100% intergenic (+3/‑114) trpF → / → trpB1 phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,200,7941.→G100.0% 108.7 193intergenic (+3/‑114)trpF/trpB1phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  new base (19/139):  ref base (31/4):  total (50/143)
Fisher's exact test strand distribution p-value = 1.44e-18
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.02e-12

Only 100 of 230 total aligned reads displayed.

GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GT.GGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889
                                                                                                         |                                                                                                  
gggCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTC...GG.Gt                                                                                                     <  2:4016725/101‑1 (MQ=255)
     gcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GG.ggggg                                                                                               >  1:1667046/1‑100 (MQ=255)
      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCGGGGGCA...GG.GG.gggggg                                                                                              >  1:1275247/1‑99 (MQ=255)
      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GT.gggggt                                                                                              <  1:2346278/101‑3 (MQ=255)
      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GG.gggggg                                                                                              >  2:1660597/1‑99 (MQ=255)
       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GG.GG.ggggggg                                                                                             >  1:4411180/1‑98 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GT.GGGG.gttt                                                                                           <  1:3087421/101‑2 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GG.gggggggg                                                                                            >  1:114732/1‑97 (MQ=255)
          ggTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTca                                                                                          <  1:2268334/101‑1 (MQ=255)
            tCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacc                                                                                        <  1:393502/101‑1 (MQ=255)
            tCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacc                                                                                        <  1:2781569/101‑1 (MQ=255)
             cGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCCGGGGTA...GGCGG.GGGG.TTTcaccc                                                                                       >  2:3112233/1‑101 (MQ=255)
              gACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GG.gggggggttccccc                                                                                      >  1:1629672/1‑91 (MQ=255)
                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcaccccca                                                                                    <  2:4217131/101‑1 (MQ=255)
                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcaccccca                                                                                    <  1:783710/101‑1 (MQ=255)
                cGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcaccccca                                                                                    <  1:4112149/101‑1 (MQ=255)
                   gtcgtcGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacac                                                                                 <  2:3431985/101‑1 (MQ=255)
                    tcgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacacc                                                                                <  2:3015951/101‑1 (MQ=255)
                    gcgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacacc                                                                                <  2:1178676/100‑1 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGTGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacaccc                                                                               <  2:2801846/101‑1 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacaccc                                                                               <  1:1886954/101‑1 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGAG..GG.GG.GGGG.GTTcacccccccaccc                                                                               >  2:3686932/1‑101 (MQ=255)
                      gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTcagccccacacccc                                                                              >  2:1591106/1‑101 (MQ=255)
                      gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTcacccccacacccc                                                                              >  1:3101700/1‑101 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTcacccccacacccccc                                                                            >  1:1941913/1‑100 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacaccccca                                                                            <  2:885435/101‑1 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTcacccccacaccccca                                                                            <  2:49439/101‑1 (MQ=255)
                         gggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTtccccccccaccccccg                                                                           >  2:71100/1‑99 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGc                                                                          <  2:4655673/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGc                                                                          <  2:4388793/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGc                                                                          <  1:4721730/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGc                                                                          <  1:4521468/101‑1 (MQ=255)
                           gggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCg                                                                         <  2:2875010/101‑1 (MQ=255)
                            ggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCAACCCCACACCCCCCGCga                                                                        >  1:3069207/1‑100 (MQ=255)
                            ggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGt                                                                        <  2:1028609/101‑1 (MQ=255)
                            ggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGAAGGGGTAG..GG.GG.GGGG.TTTCAACCACCCCCCCCCCGCgg                                                                        >  1:652626/1‑100 (MQ=255)
                              tGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTAt                                                                      <  1:3669291/101‑1 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCACACCCCCAGCGGAttt                                                                    >  2:305774/1‑101 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCGTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GGTCACCCCCCCCCCCCCCCCGCAttt                                                                    >  2:2425779/1‑101 (MQ=255)
                                 aaTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTAtttt                                                                   <  2:806736/101‑1 (MQ=255)
                                 aaTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTAtttt                                                                   <  1:3180789/101‑1 (MQ=255)
                                 aaTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTAtttt                                                                   <  1:2394074/101‑1 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCACACCCCCACCGTGTTTTg                                                                  >  2:2070383/1‑101 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTg                                                                  <  1:3882334/101‑1 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCTGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTgg                                                                 <  2:3964219/101‑1 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCACACCCCCAGCGTATTTTgg                                                                 >  2:3617517/1‑101 (MQ=255)
                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGc                                                                <  1:4674222/101‑1 (MQ=255)
                                     ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGcc                                                               <  2:4410930/101‑1 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCCCCCCCCCACCCCCCGCGGATTTTGGCCaaa                                                            >  1:4209876/1‑100 (MQ=255)
                                         cccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCaaga                                                           <  1:2554311/101‑1 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCaagaag                                                         <  2:3344956/101‑1 (MQ=255)
                                            ggggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCACACCCCCAGCGTATTTTGGCCAagaaga                                                        >  1:2178040/1‑101 (MQ=255)
                                               gTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTTACCCCCCCCCCCCCAGCGTATTTTGGGCAAGAAGaaaa                                                     >  2:1396771/1‑100 (MQ=255)
                                                tCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACa                                                    <  2:4297373/101‑1 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAgg                                                  <  1:3453018/101‑1 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAgg                                                  <  1:4599806/101‑1 (MQ=255)
                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTTACCCCCACCCCCCCCCCCCACTTTGGCCAAGAAAAAACAAGa                                                 >  2:3514677/1‑101 (MQ=255)
                                                   aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGa                                                 <  1:1927812/101‑1 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGaa                                                <  1:4339647/101‑1 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGaa                                                <  2:2177916/101‑1 (MQ=255)
                                                     gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCCCACCCCCCGCGGATTTTGGCCAAGAAGAAACAGGAAc                                               >  1:2676801/1‑101 (MQ=255)
                                                     gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAAc                                               >  1:4446354/1‑101 (MQ=255)
                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTTGCCAAGAAGAAACAGGAACtt                                             <  2:391421/101‑1 (MQ=255)
                                                       ggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACtt                                             <  2:3206475/101‑1 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACttt                                            <  1:3836718/101‑1 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACttt                                            <  2:565481/101‑1 (MQ=255)
                                                         ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.a                                          <  1:3706764/101‑1 (MQ=255)
                                                          cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.Ac                                         <  2:1537112/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.Accc                                       <  2:648535/101‑1 (MQ=255)
                                                             ccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.Acccc                                      <  1:3633008/101‑1 (MQ=255)
                                                             ccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.Acccc                                      <  2:2403350/101‑1 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCCCACCCCCAGCGGATTTTTGCCAAGGAGAAACAAGGACTTT.ACCCCg                                     >  2:4511280/1‑101 (MQ=255)
                                                                gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCCCACCCCCCCCGGATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.g                                  >  1:3142703/1‑101 (MQ=255)
                                                                 aTTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.gg                                 <  2:1941796/101‑1 (MQ=255)
                                                                  ttGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGG.TTTCCCCCCCCCCCCCCCCG.GATTTTTGGCCCAAAAAAAAAAGGAAATTT.Acccccggg                                  >  1:1706403/1‑97 (MQ=255)
                                                                     ccgccTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.gggggg                             <  1:4387631/101‑1 (MQ=255)
                                                                      cgccTTTGTCGCGGCGGCGCAGGGGTAGG.GG.GG.GGGG.GTTTACCCCCCACCCCCCCCCGAATTTTGGCCAAAAAAAAAAAGGAAATTTCACCCCGG.ggggg                              >  2:3329539/1‑101 (MQ=255)
                                                                      cgccTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.GTTTCCCCCCCCACCCCCCGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.AACCCGA.ggggggg                            >  2:3525005/1‑101 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTAG..GG.GG.GGGG.GTTCACCCCCCCACCCCCAGCGTATTTTGGCCCAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGgcg                         >  2:2186966/1‑101 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGTCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGgcg                         >  2:2319720/1‑101 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGgcg                         <  1:1348673/101‑1 (MQ=255)
                                                                          tttGTCGCGGCGGCGCAGGGGTAGGGGG.GG.GGGG.TTTCCCCCCCCCCCCCCCCCCCTTTTTTTG.CACAAAAAAAAAGAAAATTT.ACCCCCG.gggggggggg                         >  1:3427028/1‑99 (MQ=255)
                                                                           ttGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGGATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGgcgcc                       >  2:2273853/1‑100 (MQ=255)
                                                                           ttGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.GTTTACCCCCCCACCCCCAGCGGATTTTGGCCAAGAAAAAAAAGGAACTTT.ACCCCGA.GGGGGGGgcgcg                       >  2:2599552/1‑101 (MQ=255)
                                                                            tGTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGGATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGgcgcgc                      >  2:2636556/1‑101 (MQ=255)
                                                                             gTCGCGGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCt                     <  2:2005788/101‑1 (MQ=255)
                                                                               cgcgGCGGCTCAGTGGTA...GG.GT.GGGGTTTTCACCCCCACACCCCCAGCTTTTTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTAt                   <  1:106059/101‑1 (MQ=255)
                                                                               cgcgGCGGCGCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCCAGAAGAAACAGGAACTTT.CCCCCGA.GGGGGGGGCGCGCTAt                   >  2:3853977/1‑101 (MQ=255)
                                                                                gcggcggcgCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATc                  <  2:1749803/101‑1 (MQ=255)
                                                                                gcggcggcgCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATc                  <  2:2846253/101‑1 (MQ=255)
                                                                                 cggcggcgCATGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATct                 <  2:1314256/101‑1 (MQ=255)
                                                                                     ggcgCAGGGGTA...GG.GTGGGGG.TTTCACCCCCCCACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGAGGGGGGGGGCGCGCTATctgct              <  2:597017/101‑1 (MQ=255)
                                                                                     ggcgCAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATctgctg             <  2:251533/101‑1 (MQ=255)
                                                                                         cAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTgg         <  2:1276718/101‑1 (MQ=255)
                                                                                         cAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTgg         <  2:3810608/101‑1 (MQ=255)
                                                                                         cAGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTgg         <  2:1853912/101‑1 (MQ=255)
                                                                                          aGGGGTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGgc        >  1:738469/1‑101 (MQ=255)
                                                                                            gggTA...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGgcgc      <  2:3282324/101‑1 (MQ=255)
                                                                                                a...GG.GTGGGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  2:1258649/1‑101 (MQ=255)
                                                                                                 gaggg.gG.GGGG.TTGCCCCCCCACCCCCCAAGGGTTTTTGGGCAAAGAAGAAACGGAACCTTT.CCCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTg   <  1:861424/98‑1 (MQ=255)
                                                                                                         |                                                                                                  
GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GG.GT.GGGG.TTTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTT.ACCCCGA.GGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG