BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,255,985	+G	2.5%	intergenic (+155/+87)	RCAP_rcc01184 → / ← RCAP_rcc01185	CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,255,985	1	.→G	2.5%	5.4 0.0 ‑0.7	162	intergenic (+155/+87)	RCAP_rcc01184/RCAP_rcc01185	CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand): new base (2/2): ref base (77/81): total (79/83)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.81e-01

Only 100 of 185 total aligned reads displayed.


GCTGTGGTACCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.A.G.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGGCGCGC  >  NC_014034/1255895‑1256084


tgccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.gggggggggg                                                                                               >  2:3564583/11‑101 (MQ=255)


 cccccccgtcccTGCGGGCCTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGa                                                                                              <  2:3264546/92‑1 (MQ=21)


  ccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCTCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGaa                                                                                             >  1:2904472/9‑101 (MQ=255)


  ccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGaa                                                                                             <  1:1875294/93‑1 (MQ=255)


  ccccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGaa                                                                                             <  2:2687937/93‑1 (MQ=255)


    ccccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACt                                                                                           >  2:2132301/7‑101 (MQ=255)


     cccgtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACtt                                                                                          <  2:2260117/96‑1 (MQ=255)


       cgtcccTGCGGGACTCCCCCCGGAATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTcc                                                                                        <  2:3461960/98‑1 (MQ=255)


        gtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTccc                                                                                       >  2:3150134/3‑101 (MQ=255)


        gtcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTccc                                                                                       <  1:1895729/99‑1 (MQ=255)


         tcccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCg                                                                                      >  1:2379563/2‑101 (MQ=255)


          cccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCga                                                                                     <  2:3395250/101‑1 (MQ=255)


           ccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGCACTTCCTgac                                                                                    >  1:4241503/1‑100 (MQ=255)


           ccTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCga.a                                                                                   <  1:594062/101‑1 (MQ=255)


            cTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGCACTCCCCga.a.g                                                                                 >  1:1390245/1‑101 (MQ=255)


            cTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCga.a.g                                                                                 >  1:2409720/1‑101 (MQ=255)


            cTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCga.a.g                                                                                 <  2:736349/101‑1 (MQ=255)


              gCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCga.a.gga                                                                               <  1:3813975/101‑3 (MQ=255)


               ggggGCTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAGCCGGGGGCGCGGTTTTCGCCTTGGGCCAAATACCCCGGGGGGGGGGGAACTTCCCga.a.gga                                                                               <  1:1012435/100‑3 (MQ=21)


               ggggCCTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGa.a.g.gaa                                                                             <  2:1859008/100‑1 (MQ=255)


               cGGGCCCCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGa.a.g.gaa                                                                             <  1:983565/101‑1 (MQ=255)


               cGGGCCCCCCCCCGGGACATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGa.a.g.gaa                                                                             <  1:662488/101‑1 (MQ=37)


                gggACCCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGa.a.g.gaaa                                                                            <  1:1405008/101‑2 (MQ=255)


                  gccTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.agg.aaaga                                                                           <  1:1051789/99‑1 (MQ=255)


                  gagtCCCCCCGGAATTTTTCCACTAAGGGGAGACAAACCGGGGGCGCGGCTTTCTCCGTGGGCCAAATTCCCC.GGGGGGCGGGAACTTCCCGA.a.gtaaaga                                                                           <  2:2366019/98‑1 (MQ=255)


                    cTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAgg                                                                         <  1:1274394/101‑1 (MQ=255)


                    cTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAgg                                                                         >  1:3486615/1‑101 (MQ=255)


                     tCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAggg                                                                        <  1:1020108/101‑1 (MQ=255)


                      ccccccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAgggg                                                                       <  1:3772099/101‑1 (MQ=255)


                       cccccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCTCCTTGGGCAAAATTCCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAggggg                                                                      <  2:460620/101‑1 (MQ=255)


                        ccccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAgggggg                                                                     >  2:4333079/1‑101 (MQ=255)


                          ccGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCa                                                                   <  1:157283/101‑1 (MQ=255)


                           cGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGACTTTCCCGAGG.G.AAAAAGGGGGGCA.g                                                                 >  1:4474310/1‑101 (MQ=21)


                             ggATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGGA.ggg                                                               >  1:2999527/1‑101 (MQ=255)


                              gATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGc                                                              >  1:4693879/1‑101 (MQ=255)


                              gATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGc                                                              >  2:1339307/1‑101 (MQ=255)


                               atatTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGGGGGGGGCA.GGGcc                                                             >  1:695925/1‑101 (MQ=255)


                               atatTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGcc                                                             <  2:800148/101‑1 (MQ=255)


                                  tttCCGCCAGGGGGAAATGGACCCGGGGGCGGGCTTTCACCGTGGGGCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGccccc                                                          <  2:1779389/101‑1 (MQ=25)


                                  tttCCCCCAAGGGGAAAAGGACCCCGGGGGCGGCTTTCCCCCTGGGCCAAATTTCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGccccc                                                          <  2:4220391/101‑1 (MQ=255)


                                   ttCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGACCTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCt                                                         >  1:4435641/1‑101 (MQ=255)


                                   ttCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCt                                                         >  2:3810122/1‑101 (MQ=255)


                                    tCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCACCCtt                                                        <  1:2001927/101‑1 (MQ=255)


                                     ccGCCAGGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCttt                                                       <  2:1265936/101‑1 (MQ=255)


                                     ccGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCttt                                                       <  1:1968777/101‑1 (MQ=255)


                                     ccGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCttt                                                       <  2:4502310/101‑1 (MQ=255)


                                     ccGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCttt                                                       <  2:3967759/101‑1 (MQ=255)


                                      cGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTc                                                      >  1:1111302/1‑101 (MQ=255)


                                      cGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTc                                                      >  1:2859231/1‑101 (MQ=255)


                                       gCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGGA.GGGACCCCTTTcc                                                     >  2:2846215/1‑101 (MQ=37)


                                        ccAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTccc                                                    >  2:657431/1‑101 (MQ=255)


                                         cAAGGGGAAATGAACCCGGGGCGCGGCTTTGCCCGTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCg                                                   <  1:4448795/101‑1 (MQ=38)


                                         cAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCg                                                   >  1:3775171/1‑101 (MQ=255)


                                         cAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCg                                                   <  2:2556341/101‑1 (MQ=255)


                                          aaGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGa                                                  >  2:679556/1‑101 (MQ=255)


                                          aaGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGa                                                  >  1:1773660/1‑101 (MQ=255)


                                            ggggAAAAGGACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATc                                                <  1:4454646/101‑1 (MQ=38)


                                              ggAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAAAAGGGGGCA.GGGCCCCCTTTCCCCATCgg                                              >  2:4173755/1‑101 (MQ=37)


                                               gAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCggg                                             >  1:2614541/1‑101 (MQ=255)


                                                  aggAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGcc                                          <  2:1476655/99‑1 (MQ=255)


                                                  aTGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGcc                                          >  1:2726075/1‑101 (MQ=255)


                                                     aaCCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGt                                       <  2:3356109/101‑1 (MQ=255)


                                                      aCCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAATTTCCCAA.A.G.GAAAAGGGGGGGAGGGGCCCCCTTTCCCGTTGGGGGCGgggc                                      >  1:2103511/1‑99 (MQ=21)


                                                       cccGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGc                                     <  2:1703752/101‑1 (MQ=255)


                                                          ggggCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGcccc                                  >  1:2752586/1‑99 (MQ=255)


                                                          ggggCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGCTGCcgc                                  >  1:775425/1‑101 (MQ=255)


                                                            ggCGCGGCTTTCACCTTGGGCCAAATATCCCGGGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCcgcg                                 <  2:3455473/101‑1 (MQ=38)


                                                             gcgcGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCcgcgcg                               <  1:1624591/101‑1 (MQ=255)


                                                               gcgGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGc                             >  2:72440/1‑101 (MQ=255)


                                                               gcgGCTTTCACCTTGGGCCAAATA.CCC.CGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GTGCCACCTTTCCCGATCGGGGCCGGTGCCGCGCGGCa                            <  2:3717861/101‑1 (MQ=37)


                                                                cgGCTTTCACCTTGGGCCAAATATCCCGGGGGGGGGGGAACTTCCCGA.AGG.AAAAAGGGGGGCG.GGGCCCCCTTCCCCGATCGGGGCCGGGCCCGCGGGGc                             >  2:2739222/1‑101 (MQ=25)


                                                                 ggCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAg                           >  1:2908158/1‑101 (MQ=255)


                                                                   cTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAggg                         >  1:2253053/1‑101 (MQ=255)


                                                                    tttCACCTTGGGCCAAATATCCCGGGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGCCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGCCAggg                         >  1:1537007/1‑101 (MQ=38)


                                                                    tttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGt                        >  1:3901113/1‑101 (MQ=255)


                                                                     ttCCCCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTc                       <  2:4693623/101‑1 (MQ=255)


                                                                     ttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAggggc                       >  2:2410908/1‑99 (MQ=255)


                                                                     ttCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTc                       >  1:4627627/1‑101 (MQ=255)


                                                                      tCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCt                      >  1:813850/1‑101 (MQ=255)


                                                                       cgccTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCtt                     <  2:2903253/99‑1 (MQ=255)


                                                                       cACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGGGGCAGGGGCtt                     >  1:3254055/1‑101 (MQ=255)


                                                                        ccctctggCCAAATTTCCCGGGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTTCCGCGCGGCAGGGTCtt                     <  2:3603366/95‑1 (MQ=255)


                                                                           ttGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGcc                 <  1:1248829/101‑1 (MQ=255)


                                                                           ttGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGGCTTGGcc                 >  2:1748029/1‑101 (MQ=255)


                                                                           ttGGCCCAAATTTCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GTGCCCCCTTTCCCGATCGTGGCCGGTGCCGCGCGGCAGGGTCTTGGcc                 <  2:4424911/101‑1 (MQ=255)


                                                                           tggggCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGcc                 <  2:2725921/99‑1 (MQ=255)


                                                                            tGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCg                >  1:1265505/1‑101 (MQ=255)


                                                                             gggCCAAATAGCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCgg               <  2:2999339/101‑1 (MQ=255)


                                                                              ggCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGGGGCAGGGGCTTGGCCggg              >  1:4557840/1‑100 (MQ=39)


                                                                                ccAAATATCCG.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCa            <  1:1196207/101‑1 (MQ=255)


                                                                                 cAAATATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAg           <  2:3930528/101‑1 (MQ=255)


                                                                                  aaaTATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGGGGGGGGAA.GGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGGCTTGGCCGGTCAgg          >  2:1986985/1‑101 (MQ=255)


                                                                                   aaTATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGCCTTGGCCGGTCAggc         >  2:1635011/1‑101 (MQ=255)


                                                                                   aaTATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGGGCCGCGCGGCAGGGGCTTGGCCGGTCAggc         >  1:3616121/1‑101 (MQ=255)


                                                                                   aaTATCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCCATCGGGGCCGGCGCCCGGCGGCAGGGCCTTAGCCGGTCAggc         >  2:1135176/1‑101 (MQ=255)


                                                                                      atCCC.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGGCTTGGCCGGTCAggcggc      >  2:2609287/1‑101 (MQ=255)


                                                                                        ccc.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGGGgcgc    >  1:4573138/1‑101 (MQ=255)


                                                                                        ccc.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgc    >  1:38777/1‑101 (MQ=255)


                                                                                         cc.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcg   <  2:2484396/101‑1 (MQ=255)


                                                                                          c.GGGGGGGGGGAACTTCCCGA.AGG.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGgcgcgc  >  1:3438737/1‑101 (MQ=255)


GCTGTGGTACCCCTGCGGGACTCCCCCCGGGATATTTCCGCCAAGGGGAAATGAACCCGGGGCGCGGCTTTCACCTTGGGCCAAATATCCC.GGGGGGGGGGAACTTCCCGA.A.G.AAAGAGGGGGGCA.GGGCCCCCTTTCCCGATCGGGGCCGGTGCCGCGCGGCAGGGTCTTGGCCGGTCAGGCGGCGCGC  >  NC_014034/1255895‑1256084

Base quality scores:


ATCG

< 3 ≤


ATCG

< 12 ≤


ATCG

< 23 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG