Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,320,647 +G 97.7% intergenic (+105/+80) potI1 → / ← RCAP_rcc01247 polyamine ABC transporter permease PotI/membrane transport family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,320,6471.→G97.7% 6.8 0.0 ‑0.6215intergenic (+105/+80)potI1/RCAP_rcc01247polyamine ABC transporter permease PotI/membrane transport family protein
Reads supporting (aligned to +/- strand):  new base (130/80):  ref base (3/2):  total (133/82)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.26e-01

Only 100 of 238 total aligned reads displayed.

GCCTGCCAGATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.G.G.CA.G.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATC  >  NC_014034/1320547‑1320739
                                                                                                     |                                                                                                    
   tGCCAGATCCGCACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCTCAAAATTCACTCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.g.gg                                                                                                  >  2:3143333/1‑100 (MQ=255)
    gCCAGATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.g.ggc                                                                                                 <  1:3362993/101‑3 (MQ=255)
    gCCAGATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAAATGAAA.g.ggc                                                                                                 >  1:2449417/1‑99 (MQ=255)
         aTCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CA.g.g.gg                                                                                         <  2:1699222/101‑2 (MQ=255)
           ccGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CA.g.g.ggct                                                                                       <  1:1109632/101‑4 (MQ=255)
            cGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CA.g.g.ggctg                                                                                      >  2:3073888/1‑97 (MQ=255)
             gAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CA.g.g.ggctgc                                                                                     >  1:346917/1‑96 (MQ=255)
               aCCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.g.ct.gcgc                                                                                  >  1:1783205/1‑101 (MQ=25)
                 cGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.g.ct.gcgctg                                                                                <  1:4307721/101‑1 (MQ=37)
                   cAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGcc                                                                              <  1:1942644/101‑1 (MQ=37)
                     gAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGCGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.AT.GCGCTGCCCt                                                                            >  1:467682/1‑101 (MQ=37)
                     gAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCt                                                                            <  1:3745573/101‑1 (MQ=25)
                     gAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCGGCCCt                                                                            >  2:2132809/1‑101 (MQ=25)
                     gAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGAATATTTGAAGCACGGTGAAACTGAAAGG.G.CA.G.G.G.GTCACGCTGCCCt                                                                            >  2:2804242/1‑101 (MQ=37)
                      aaaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCtt                                                                           <  2:838236/101‑1 (MQ=37)
                      aaaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCtt                                                                           <  1:446216/101‑1 (MQ=37)
                       aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTc                                                                          <  1:4265435/101‑1 (MQ=37)
                        aGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCa                                                                         <  2:3831006/101‑1 (MQ=37)
                        aGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GGGCTGGCCTTCa                                                                         >  2:2056914/1‑101 (MQ=37)
                        aGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTAACCCCCCCAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCa                                                                         >  1:3521724/1‑101 (MQ=38)
                         ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCAt                                                                        <  2:2415112/101‑1 (MQ=37)
                         ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCAt                                                                        <  2:2308361/101‑1 (MQ=37)
                         ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCAt                                                                        >  2:2143945/1‑101 (MQ=37)
                          gggggCTGTCTTCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATc                                                                       <  2:703199/101‑1 (MQ=37)
                          gggggCTGTCTGCCCCCCTCTTTTTCTGCGCCAAAATTACCCCCCCGGGATATTTGAACGAAGGGGAAACTGAAAGG.G.CAGG.G.G.GT.GCGCCGCCCTTcctt                                                                       >  2:1686363/1‑98 (MQ=16)
                            gggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCtt                                                                     >  2:471040/1‑101 (MQ=38)
                               cTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGcc                                                                  <  1:3206708/101‑1 (MQ=38)
                                tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCca                                                                 >  2:3844169/1‑101 (MQ=38)
                                tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCCTCATCTTGccc                                                                 >  1:1727448/1‑100 (MQ=37)
                                 gTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCcac                                                                >  1:449603/1‑101 (MQ=38)
                                  tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGGTTCCCTTCATTTTGCccca                                                               >  2:1450149/1‑101 (MQ=25)
                                  tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCcaca                                                               <  2:2578699/101‑1 (MQ=38)
                                  tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCcaca                                                               >  2:4600765/1‑101 (MQ=38)
                                      ccccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAata                                                           <  1:4356222/101‑1 (MQ=38)
                                        ccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATc                                                         >  1:2197952/1‑101 (MQ=39)
                                          ccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATccc                                                       <  1:2212754/101‑1 (MQ=38)
                                          ccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATccc                                                       <  2:2432647/101‑1 (MQ=38)
                                            tctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCgg                                                     >  1:4612513/1‑101 (MQ=39)
                                            tctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCgg                                                     >  1:783834/1‑101 (MQ=38)
                                             ctTTTGCTGCGCAAATTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCggg                                                    >  2:3933184/1‑101 (MQ=38)
                                             ctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCggg                                                    >  1:490581/1‑101 (MQ=38)
                                             ctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCggg                                                    >  2:1011909/1‑101 (MQ=39)
                                              ttttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCgggg                                                   >  1:3096358/1‑101 (MQ=39)
                                               tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                  >  2:636263/1‑101 (MQ=38)
                                               tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                  >  1:2869448/1‑101 (MQ=39)
                                                ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                 <  1:1652232/101‑1 (MQ=38)
                                                ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                 >  2:4415388/1‑101 (MQ=38)
                                                ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                 >  1:1331662/1‑101 (MQ=38)
                                                 tgctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGa                                                >  2:2984821/1‑101 (MQ=38)
                                                      cgcAAAATTCACGCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.GTG.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGtaga                                           >  1:2580124/1‑98 (MQ=37)
                                                      cgcAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTccc                                           >  2:3394898/1‑101 (MQ=38)
                                                       tcAAAATTCGTCTCCCGAGGATATTTGAAGCAAGGTTATACTGAAATG.G.CA.GAG.G.TT.GCGCTGCCCTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCCg                                          <  2:460628/100‑1 (MQ=21)
                                                         aaaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCa                                        >  2:1310405/1‑101 (MQ=37)
                                                         aaaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCa                                        >  2:2073012/1‑101 (MQ=37)
                                                         aaaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCa                                        >  2:1844144/1‑101 (MQ=37)
                                                          aaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAg                                       >  2:1389022/1‑101 (MQ=37)
                                                           aaTTCCCCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAgg                                      <  1:71528/101‑1 (MQ=37)
                                                           aaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAgg                                      >  1:2345509/1‑101 (MQ=37)
                                                            aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                     <  1:3779103/101‑1 (MQ=37)
                                                            aTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAggg                                     <  2:2307102/101‑1 (MQ=37)
                                                             ttCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGa                                    >  2:152508/1‑101 (MQ=37)
                                                              tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                   <  2:4509578/101‑1 (MQ=37)
                                                              tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                   >  2:578410/1‑101 (MQ=37)
                                                               cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                 >  1:624287/1‑101 (MQ=25)
                                                                cccccccGTGGATATTTGAAGTAAGGTGAAACTGAAA.G.GTGA.G.GTG.CT.GCGCTGCCCTTCATCTTGCCGCAAATTTCCCGGGGGGAGTCCCGCAGGGAC.gg                                <  1:3069841/100‑1 (MQ=21)
                                                                cccccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gg                                <  2:76095/100‑1 (MQ=25)
                                                                   ccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggg                             >  1:3246354/1‑101 (MQ=25)
                                                                     ccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                           <  2:233054/101‑3 (MQ=21)
                                                                      cGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggca                          <  2:449559/101‑4 (MQ=21)
                                                                        aGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                        <  1:3933426/101‑6 (MQ=21)
                                                                        aGGATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                        <  2:1331570/101‑6 (MQ=21)
                                                                          gATATTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcggcgc                      >  1:3982226/1‑94 (MQ=18)
                                                                           atatTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                      >  1:573146/1‑101 (MQ=18)
                                                                           atatTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                      >  1:2043040/1‑101 (MQ=18)
                                                                           atatTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                      >  2:2659950/1‑101 (MQ=18)
                                                                            tatTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                     >  2:2619251/1‑101 (MQ=18)
                                                                            tatTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                     <  1:2328879/101‑1 (MQ=18)
                                                                             atTTGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcccc                    <  1:2985009/101‑1 (MQ=18)
                                                                              tttGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccccc                   >  2:4139824/1‑101 (MQ=18)
                                                                               ttGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                  <  2:1153275/101‑1 (MQ=18)
                                                                               ttGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCt                  >  2:410737/1‑101 (MQ=18)
                                                                                tGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCtt                 <  2:3647492/101‑1 (MQ=18)
                                                                                tGAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCtt                 <  1:3626869/101‑1 (MQ=18)
                                                                                 gAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTg                >  1:2827534/1‑101 (MQ=18)
                                                                                 gAAGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTg                >  2:957111/1‑101 (MQ=18)
                                                                                   aGCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCt              <  1:1312946/101‑1 (MQ=18)
                                                                                    gCAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAAAATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCtt             <  1:3140038/101‑1 (MQ=18)
                                                                                     cAAGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTa            <  1:1844256/101‑1 (MQ=18)
                                                                                      aaGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCCTGCTTAc           >  2:2191007/1‑101 (MQ=17)
                                                                                       aGGTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATGTGGCCACAAATATCCCGGAGGGAGTCCCGCAGGGACGGGGGGCACCGCCCCCTTGCTTACg          >  2:794003/1‑101 (MQ=18)
                                                                                        ggTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACgg         <  2:412314/101‑1 (MQ=18)
                                                                                        ggTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACgg         >  2:4089173/1‑101 (MQ=18)
                                                                                        ggTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACgg         <  1:2267000/101‑1 (MQ=18)
                                                                                         gTGAAACTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGc        <  2:2855158/101‑1 (MQ=18)
                                                                                            tctcTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATGCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAgg     <  2:3130322/98‑1 (MQ=17)
                                                                                             aaCTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGT.CCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAgggg   >  1:847399/1‑99 (MQ=18)
                                                                                               cTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGacc  >  2:3609581/1‑99 (MQ=17)
                                                                                               cTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATc  >  2:2823506/1‑101 (MQ=18)
                                                                                               cTGAAAGG.G.CAGG.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATc  <  1:4628211/101‑1 (MQ=18)
                                                                                               cTGAAA.GTG.CA.G.G.GTCT.GCGCTGCCCTTCATCTTGCCACATATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATc  <  2:4204779/101‑1 (MQ=18)
                                                                                                     |                                                                                                    
GCCTGCCAGATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.G.G.CA.G.G.G.CT.GCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATC  >  NC_014034/1320547‑1320739

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG