Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,426 +T 5.7% intergenic (+238/‑693) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4261.→T 5.7% 37.0 ‑1.5 ‑0.5194intergenic (+238/‑693)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (102/81):  total (106/88)
Fisher's exact test strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.18e-01

Only 100 of 246 total aligned reads displayed.

GCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395326‑1395526
                                                                                                      |                                                                                                    
 cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                     <  1:2718479/101‑1 (MQ=255)
 cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                     >  1:1431060/1‑101 (MQ=255)
 cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                     >  2:4648326/1‑101 (MQ=255)
 cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgggg.a                                                                                                     >  1:1240469/1‑101 (MQ=255)
  aaTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGCGGGGGGTCGAAACTGCACCGCTTGGCCCCGCcggtggccggtgtt                                                                                                     >  1:2891482/1‑99 (MQ=255)
   aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                                   >  1:1777387/1‑101 (MQ=255)
    tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                  <  2:4181581/101‑1 (MQ=255)
     tttccccGGCTGGTCCGGGGGTTTCGGAACGGGAGTCGCCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                  <  1:122525/96‑1 (MQ=25)
     ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                 <  2:2822575/101‑1 (MQ=255)
     ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                 >  1:1576605/1‑101 (MQ=255)
     ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                 <  1:3222068/101‑1 (MQ=255)
     ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                                 >  2:4028171/1‑101 (MQ=255)
       acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCg                                                                                               >  2:2692648/1‑101 (MQ=255)
        cacCGTCTGGTGAGGGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAGCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCgg                                                                                              <  2:3269012/101‑1 (MQ=38)
          ccGTCTGGTCAGCGTGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                            <  2:3118557/101‑1 (MQ=255)
          ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                            <  1:499883/101‑1 (MQ=255)
           cGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                                           >  2:1526228/1‑101 (MQ=255)
            gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                          <  2:901034/101‑1 (MQ=255)
            gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                          >  1:1542723/1‑101 (MQ=255)
            gTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                          >  1:1128238/1‑101 (MQ=255)
             tCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                                         <  2:3014691/101‑1 (MQ=255)
             tCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                                         <  1:1888895/101‑1 (MQ=255)
                ggTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                                      <  1:3285908/101‑1 (MQ=255)
                 gTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTcccc                                                                                     >  2:859224/1‑101 (MQ=255)
                  tCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                    <  1:1565066/101‑1 (MQ=255)
                  tCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                                    <  2:2449562/101‑1 (MQ=255)
                    aGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                                  >  1:4362631/1‑101 (MQ=255)
                       gggTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                                               >  1:946309/1‑101 (MQ=255)
                         gTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                                             <  2:3851517/101‑1 (MQ=255)
                          ttACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggggg                                                                            >  2:3899445/1‑101 (MQ=255)
                           tACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                           >  2:4307418/1‑101 (MQ=255)
                           tACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                                           >  2:210988/1‑101 (MQ=255)
                            aCGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                                                          >  2:1658087/1‑101 (MQ=255)
                              gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:1745134/101‑1 (MQ=255)
                              gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:3805763/101‑1 (MQ=255)
                              gAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                        >  1:3764686/1‑101 (MQ=255)
                              caaccGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                                        <  2:2555237/97‑1 (MQ=255)
                               tcaacactgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     >  1:1142741/9‑101 (MQ=2)
                               aaaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGGG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTgg                                                                       >  1:1392815/1‑101 (MQ=255)
                                aaCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                      <  2:4427497/101‑1 (MQ=255)
                                 aCGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGaa                                                                     >  2:839720/1‑100 (MQ=255)
                                      tgTCG.CTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                              <  2:364363/100‑2 (MQ=2)
                                      aGTCG.CCCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:403347/101‑1 (MQ=255)
                                       gTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  2:2404470/1‑101 (MQ=255)
                                        tCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                              >  1:3556467/1‑101 (MQ=255)
                                        tCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                              >  1:1952128/1‑101 (MQ=255)
                                         cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                             >  1:678747/1‑101 (MQ=255)
                                         cG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                             >  1:2278449/1‑101 (MQ=255)
                                          g.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                            >  2:4056848/1‑101 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                          >  2:4343137/1‑101 (MQ=255)
                                             cccccccGCGGGGGCGTNGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                          <  2:1542622/100‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccg                                                        <  1:2376186/101‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGGCGCTCCCCCCCCGGGGGCGAGGAGCGAAACATcgccg                                                        >  2:4262527/1‑101 (MQ=39)
                                                ccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgccgc                                                       <  1:1042531/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                    <  2:4602113/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                    <  1:4162914/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                   <  1:2125642/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGGCGCTCCCCCCGCGGGGGCGTCGATCGAAACATCGCCTCCTTggg                                                 >  2:1805213/1‑100 (MQ=38)
                                                       ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                                >  2:2538927/1‑101 (MQ=255)
                                                        gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                               <  2:3446393/101‑1 (MQ=255)
                                                         ggCGTGTATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                              <  2:3491787/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                              >  1:3108544/1‑101 (MQ=255)
                                                         ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                              >  1:1904689/1‑101 (MQ=255)
                                                          gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                                             >  2:292233/1‑101 (MQ=255)
                                                            gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                           >  2:2910573/1‑101 (MQ=255)
                                                             tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                          <  2:1169461/101‑1 (MQ=255)
                                                             tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                          >  2:4190470/1‑101 (MQ=255)
                                                               gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                        >  1:4496414/1‑101 (MQ=255)
                                                                aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                       >  1:1601440/1‑101 (MQ=255)
                                                                  cGAAACTGCCCCCGCGGGCCCTGCCGGTGATCGGTG.ACCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                     <  1:3304601/101‑1 (MQ=255)
                                                                   gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                    >  1:2466069/1‑101 (MQ=255)
                                                                    aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                   >  2:1999207/1‑101 (MQ=255)
                                                                    aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                   <  2:805090/101‑1 (MQ=255)
                                                                    aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                   <  1:261324/101‑1 (MQ=255)
                                                                    aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                   <  2:3598960/101‑1 (MQ=255)
                                                                      aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                 <  1:2939327/101‑1 (MQ=255)
                                                                      aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                 <  2:2031517/101‑1 (MQ=255)
                                                                      aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                 >  1:87160/1‑101 (MQ=255)
                                                                        tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAAc                               >  1:4724104/1‑101 (MQ=255)
                                                                         gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                              >  2:472824/1‑101 (MQ=255)
                                                                         gCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                              >  2:100228/1‑101 (MQ=255)
                                                                               gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                        >  1:3366984/1‑101 (MQ=255)
                                                                               gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                        <  2:1941668/101‑1 (MQ=255)
                                                                                cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCt                       >  2:4518900/1‑101 (MQ=255)
                                                                                 tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                      >  1:3916197/1‑101 (MQ=255)
                                                                                 tGGCCCTGCCGGTGATCGGTA.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                      <  2:119426/101‑1 (MQ=255)
                                                                                   gCCCTGGCGGGGATCGGTG.ATCAGCGGTCCCTCCCCCCGCGGGGCCGCGGCTCGGAACATGGCCGCTTTGGCGGTCGACATCCTGGGGACgtgcgcgtccc                    >  2:236239/1‑93 (MQ=255)
                                                                                   gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                    <  1:4690757/101‑1 (MQ=255)
                                                                                   gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                    <  1:79447/101‑1 (MQ=255)
                                                                                      cTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                 <  2:1240379/101‑1 (MQ=255)
                                                                                       tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccccc                >  2:2491608/1‑100 (MQ=255)
                                                                                       tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                >  2:3207878/1‑101 (MQ=255)
                                                                                       tGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGCAACGTGTCGCTCCCCCcg                >  1:1706350/1‑101 (MQ=255)
                                                                                          cggtgatcggtg.atCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg             >  2:2184999/1‑101 (MQ=255)
                                                                                            gtgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg           <  1:3381087/101‑1 (MQ=255)
                                                                                             tgatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg          <  1:295225/101‑1 (MQ=255)
                                                                                              gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc         >  1:1805842/1‑101 (MQ=255)
                                                                                              gatcggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc         <  1:3692393/101‑1 (MQ=255)
                                                                                                  ggtg.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg     <  2:568243/101‑1 (MQ=255)
                                                                                                     g.atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc  <  2:3764451/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
GCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCG.CTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395326‑1395526

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG