Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,028 +G 20.9% intergenic (+840/‑91) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0281.→G20.9% 174.5 ‑0.5 ‑0.5244intergenic (+840/‑91)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (25/26):  ref base (86/107):  total (111/133)
Fisher's exact test strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.29e-01

Only 100 of 268 total aligned reads displayed.

ACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395929‑1396127
                                                                                                    |                                                                                                     
 cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cg                                                                                                     <  1:1897519/101‑1 (MQ=255)
  gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.cgc                                                                                                    <  2:2906323/101‑1 (MQ=255)
     gCGTTCATGGCCTACGTCGGGCAGGGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTc                                                                                               <  2:879863/101‑1 (MQ=255)
          cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcc                                                                                          >  1:859291/1‑101 (MQ=255)
          cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcc                                                                                          <  2:275834/101‑1 (MQ=255)
             ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTccccc                                                                                       >  1:832245/1‑101 (MQ=255)
              gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTcccccc                                                                                      >  1:4721398/1‑101 (MQ=255)
               ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                     <  2:4750750/101‑1 (MQ=255)
                 tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                   <  1:4636794/101‑1 (MQ=255)
                  aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCGGCGCTTGCCTCACGCCTGC.CGC..GTCCCCCCCCCCGCgg                                                                                  >  1:2289010/1‑101 (MQ=255)
                  aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCgg                                                                                  >  2:1053877/1‑101 (MQ=255)
                   cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTCGGCCTCGATCCGCGCGTGACTCACGCCTGC.CGC..CACGCTACCCCCGCggg                                                                                 >  2:2284852/1‑101 (MQ=37)
                     tCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCggggg                                                                               <  2:401538/101‑1 (MQ=255)
                     tCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCggggg                                                                               <  2:2184017/101‑1 (MQ=255)
                        ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                            <  1:1814025/101‑1 (MQ=255)
                         gCAGTGGCCTGGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTg                                                                           <  2:2509259/101‑1 (MQ=255)
                         gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGCCCTCGATCCGCGCTTGCCTCACCCCTGC.CGC..GGCGCTCCCCCCGCggggggcgg                                                                           >  1:850513/1‑97 (MQ=38)
                           cgTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:4470887/100‑1 (MQ=255)
                           aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:177725/101‑1 (MQ=255)
                           aGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  2:832172/101‑1 (MQ=255)
                            gTGGGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  1:3981755/101‑1 (MQ=255)
                            gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:3149491/1‑101 (MQ=255)
                            gTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  1:1004555/101‑1 (MQ=255)
                             ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  2:1637025/7‑101 (MQ=2)
                             ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:2240109/95‑1 (MQ=9)
                             ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:1834317/7‑101 (MQ=9)
                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  2:2543597/6‑101 (MQ=2)
                               gtcTGGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCCCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCCCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:850439/99‑1 (MQ=255)
                               gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:3100275/97‑1 (MQ=9)
                               gACTGGTCGCTCCCGCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  2:1112259/1‑101 (MQ=255)
                               gACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  2:2703311/1‑101 (MQ=255)
                                cgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:3769493/98‑1 (MQ=2)
                                aCTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:962473/101‑1 (MQ=255)
                                  tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CCC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  >  1:4111942/1‑101 (MQ=255)
                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                  <  1:1385337/101‑1 (MQ=2)
                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                 <  2:2222146/101‑1 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                 <  1:3054694/101‑2 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                                <  2:1814144/101‑1 (MQ=255)
                                    gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                                <  1:1331592/101‑1 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTTGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                                >  1:4545124/1‑99 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                                >  1:3547491/1‑99 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActt                                                                >  1:291247/1‑99 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                               <  2:4342136/101‑4 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                                              <  1:4235447/101‑5 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtga                                                              <  2:3391550/101‑5 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                             >  2:2019108/1‑101 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                             >  2:177627/1‑101 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                                             <  2:4178001/101‑1 (MQ=255)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCACGCGGGGGCGTGGATCGAAACAGgcg                                                             >  2:495567/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTGGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCGCCCGCGGGGGCGCGGATCGAAACAgggga                                                            >  2:3121550/1‑98 (MQ=38)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgat                                                             >  2:2396717/1‑96 (MQ=2)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                            <  1:656913/101‑1 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                           >  2:1073545/1‑101 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACCCGCCCTCGATCCGCGCTTGCCCCACGCCTGC.GGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                          <  2:2424579/101‑1 (MQ=255)
                                           cccccGCGGGGGCGTGGATCGAAACTCGGCNTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                          <  1:343683/101‑9 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                         <  1:2395349/101‑10 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                         >  2:1963277/1‑92 (MQ=2)
                                             cccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacca                                                        <  2:3727209/101‑11 (MQ=2)
                                             cccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacca                                                        >  1:1695958/1‑91 (MQ=2)
                                                gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                    >  1:39249/1‑101 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                    <  2:3229130/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                                   <  2:1143967/101‑1 (MQ=255)
                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                                  <  1:2156697/101‑1 (MQ=255)
                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                                  >  1:582953/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                                 >  2:1367225/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                                 <  1:2731202/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                                <  1:1363187/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                                >  1:2367390/1‑101 (MQ=255)
                                                     ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                               <  1:4208691/101‑1 (MQ=255)
                                                      gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                              >  1:4409809/1‑101 (MQ=255)
                                                      gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                              <  1:776149/101‑1 (MQ=255)
                                                       cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                             >  1:3305346/1‑101 (MQ=255)
                                                       cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                             <  2:3346026/101‑1 (MQ=255)
                                                        gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                            >  1:2129515/1‑101 (MQ=255)
                                                           gATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCgg                                         <  1:308101/101‑1 (MQ=255)
                                                            aTCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                                        <  1:2054335/101‑1 (MQ=255)
                                                             tCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                       <  2:1579097/101‑1 (MQ=255)
                                                               gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCTGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                     <  1:4016127/101‑1 (MQ=255)
                                                               gAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                     >  1:2657605/1‑101 (MQ=255)
                                                                aaaCTCGCCCTGGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACAGGTGCATCTGATGATTATGTCCGGTgaga                                    >  2:3149029/1‑101 (MQ=37)
                                                                 aaCTCGCCCTCCATCCGCGCTTGCCCCACGCCAGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                                   <  1:2504419/101‑1 (MQ=255)
                                                                      ggccTCGATCCGCGCTTGGCTCCCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg                              <  2:1440003/99‑1 (MQ=255)
                                                                        ccTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                            <  2:3547852/101‑1 (MQ=255)
                                                                            gATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                        >  1:371506/1‑101 (MQ=255)
                                                                            gATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                        <  1:3860529/101‑1 (MQ=255)
                                                                              tCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTNAGACGGCTGTCGCTcnc                      >  2:1508748/1‑101 (MQ=255)
                                                                               ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                     >  1:1135476/1‑101 (MQ=255)
                                                                               ccGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                     <  2:686383/101‑1 (MQ=255)
                                                                                  cccTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg                  <  2:1202749/99‑1 (MQ=255)
                                                                                    cTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg                <  1:3736485/101‑1 (MQ=255)
                                                                                     ttGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgg               >  2:2903557/1‑101 (MQ=255)
                                                                                         cTCACGCCTGC.CGC..GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc           <  1:1053956/101‑1 (MQ=255)
                                                                                           cACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGGGAGCCGGCGGTCGCCCCCCCCGCGGGGCCgg         >  2:4388490/1‑100 (MQ=37)
                                                                                            aCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTg        >  2:4681798/1‑101 (MQ=255)
                                                                                            aCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTg        <  2:2367257/101‑1 (MQ=255)
                                                                                             cGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTgg       <  2:4406922/101‑1 (MQ=255)
                                                                                              gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa      <  1:800371/101‑1 (MQ=255)
                                                                                              gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa      <  2:2129394/101‑1 (MQ=255)
                                                                                               ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt     <  2:3913655/101‑1 (MQ=255)
                                                                                                cTGC.CGC..GTCGCT.CCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATTTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCCGGGGCGTGGATCg   >  2:1540767/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
ACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1395929‑1396127

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG