BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,968	+C	10.8%	coding (850/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,968	1	.→C	10.8%	123.3 ‑0.9 ‑0.3	369	coding (850/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (22/18): ref base (155/174): total (177/192)
Fisher's exact test strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.22e-01

Only 100 of 419 total aligned reads displayed.


CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGAAACTGC  >  NC_014034/1396868‑1397068


cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg                                                                                                          >  2:2828122/1‑101 (MQ=255)


cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg                                                                                                          >  1:2696866/1‑101 (MQ=255)


 ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg.g                                                                                                        >  2:3243383/1‑101 (MQ=255)


  gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCC.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.Gt                                                                                                       <  1:2685880/101‑1 (MQ=255)


   tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                                      >  1:675995/1‑101 (MQ=255)


        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                                                 <  1:4014425/101‑1 (MQ=255)


        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                                                 <  2:1687958/101‑1 (MQ=255)


        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                                                 >  2:2147646/1‑101 (MQ=255)


         gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                                                <  1:816500/101‑1 (MQ=255)


           tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTccccc                                                                                              >  1:1381005/1‑101 (MQ=255)


            aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                             <  1:3961692/101‑1 (MQ=255)


                     ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                                    >  1:1966654/1‑101 (MQ=255)


                     ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                                    <  1:2234545/101‑1 (MQ=255)


                       aCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCCCTCCCCCCGCGGGGGCggg                                                                                  >  2:1004574/1‑99 (MQ=255)


                         tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                                 <  2:1250707/92‑1 (MQ=0)


                         cgtggatccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                                >  2:3892539/11‑101 (MQ=0)


                          tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                               <  1:463052/101‑1 (MQ=255)


                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              >  1:3397895/9‑101 (MQ=2)


                           ccccAGACCTCGCTCCCCCGGGGGGGGCGTGGATGGAAACG.GCCT.GCCCG.GC.CGCA.GCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              <  1:2945353/98‑1 (MQ=25)


                           acccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                                 >  2:1492201/8‑101 (MQ=0)


                             ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  2:3985152/96‑1 (MQ=2)


                             ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  1:4482133/96‑1 (MQ=2)


                             ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  2:4217979/96‑1 (MQ=2)


                             gccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                            <  1:3354320/95‑1 (MQ=2)


                             aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGGGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCACGGGCGCGCGGacca                                                                             >  2:2367170/7‑98 (MQ=2)


                             aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             >  2:1380891/7‑101 (MQ=9)


                              gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                            <  2:2597278/97‑1 (MQ=2)


                              cgcccGTCCCTCCCCCCCCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              <  2:2263893/97‑1 (MQ=2)


                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           <  2:1997270/101‑1 (MQ=255)


                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGGTCGaa                                                                           >  2:4681565/1‑101 (MQ=255)


                               ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           <  1:389107/98‑1 (MQ=2)


                               gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  2:1803783/98‑1 (MQ=2)


                               cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  1:1234552/5‑101 (MQ=9)


                                tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          <  1:4066413/99‑1 (MQ=2)


                                 gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                        <  1:3543122/100‑1 (MQ=255)


                                 ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                         >  1:2421597/3‑101 (MQ=9)


                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                        <  2:3392494/99‑2 (MQ=2)


                                  tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                        >  1:163142/2‑100 (MQ=9)


                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                                       >  2:3054506/1‑99 (MQ=9)


                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                                       >  1:3064434/1‑100 (MQ=2)


                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                                                     <  2:4044688/101‑5 (MQ=2)


                                      gcgccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCCCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                                    <  1:1324092/98‑6 (MQ=9)


                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                                   >  1:738416/1‑95 (MQ=2)


                                      ccTCCCGCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                                    <  1:790088/100‑6 (MQ=2)


                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                                                  >  1:1848544/1‑101 (MQ=255)


                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                                   <  2:3733892/101‑7 (MQ=9)


                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                                                   <  2:4465160/101‑7 (MQ=9)


                                       cTCCCCCCGCGGGGGCGTAGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCACCGCGGGGGCGTGGATCGAAActacaccg                                                                  >  2:802017/1‑94 (MQ=2)


                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                                    >  2:1767536/1‑100 (MQ=2)


                                        tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                                  >  2:1726268/1‑94 (MQ=9)


                                        tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCC.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                                  >  1:1468121/1‑94 (MQ=9)


                                        cccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                                  <  1:1000110/100‑8 (MQ=9)


                                         ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                                <  2:2047798/101‑1 (MQ=255)


                                           ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                                                 <  1:4161334/101‑5 (MQ=2)


                                            cccGCGGGGGCGTGGATCGAAACA.GCCG.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tcg                                                            <  2:3455038/101‑1 (MQ=255)


                                             ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                               >  1:1094457/1‑95 (MQ=0)


                                              cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                              >  1:4441830/1‑94 (MQ=0)


                                              cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                              <  1:2079145/101‑8 (MQ=0)


                                                  ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTc                                                      <  2:4096985/101‑1 (MQ=255)


                                                  ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTc                                                      <  2:675931/101‑1 (MQ=255)


                                                   gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                     <  1:1143073/101‑1 (MQ=255)


                                                    ggCGTGCATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAg                                                    <  2:1637574/101‑1 (MQ=255)


                                                     gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGc                                                   <  1:2402294/101‑1 (MQ=255)


                                                      cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                  >  1:159035/1‑101 (MQ=255)


                                                        tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCgg                                                <  1:888234/101‑1 (MQ=255)


                                                        tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCgg                                                >  1:3472053/1‑101 (MQ=255)


                                                         ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGa                                               >  2:3182543/1‑101 (MQ=255)


                                                          gATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAc                                              <  2:3312738/101‑1 (MQ=255)


                                                          gATCGAAACC.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAc                                              <  2:1133588/101‑1 (MQ=255)


                                                               aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                        <  2:2717255/101‑1 (MQ=255)


                                                                 aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTcc                                      >  1:3129731/1‑101 (MQ=255)


                                                                   cacccT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCg                                     <  2:773028/98‑1 (MQ=255)


                                                                     gCCT.GCGCG.GC.CCCC.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTc                                   <  2:606855/101‑1 (MQ=255)


                                                                       cT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGc                                 <  2:3851075/101‑1 (MQ=255)


                                                                          gcgcG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTc                               <  2:4337311/101‑1 (MQ=255)


                                                                          gcgcG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTc                               >  2:3526122/1‑101 (MQ=255)


                                                                           cgcg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcc                              <  1:3798402/101‑1 (MQ=255)


                                                                             cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccc                            <  1:3238880/101‑1 (MQ=255)


                                                                             cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccc                            >  1:3640632/1‑101 (MQ=255)


                                                                              g.gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTccccc                           <  1:2828287/101‑1 (MQ=255)


                                                                                gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccccc                          <  2:866586/101‑1 (MQ=255)


                                                                                gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccccc                          <  2:1469522/101‑1 (MQ=255)


                                                                                gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcccccc                          >  2:2991287/1‑101 (MQ=255)


                                                                                     cA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCg.g                     <  2:2433963/101‑1 (MQ=255)


                                                                                          ggCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGc                 >  1:4246876/1‑101 (MQ=255)


                                                                                            cT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCgg               >  1:2529681/1‑100 (MQ=255)


                                                                                            cT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGt               >  1:2621771/1‑101 (MQ=255)


                                                                                             t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTg              >  1:2142538/1‑101 (MQ=255)


                                                                                               tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.GCGTTGGTCAGCCGGAC.GACGCCCCCCGCTCCCCCCGCGCGGGGCgcg              >  2:4641355/1‑99 (MQ=25)


                                                                                               tCCGC.CTG..CGGG.GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTgg             <  1:2343954/101‑1 (MQ=255)


                                                                                                 cGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGAt           <  1:3001295/101‑1 (MQ=255)


                                                                                                    gatcccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCG.GGGGCGTgg             >  2:2505418/10‑101 (MQ=2)


                                                                                                    gaccccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCG.GGGGCGTgg             <  2:511974/92‑1 (MQ=2)


                                                                                                     cTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGa        <  2:3643761/101‑1 (MQ=255)


                                                                                                          gccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGaa       <  2:3787836/98‑1 (MQ=2)


                                                                                                          cGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGAAAc     >  2:279196/1‑101 (MQ=255)


                                                                                                          cGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGAAAc     >  1:152132/1‑101 (MQ=255)


                                                                                                          cGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGAAAc     >  1:4743276/1‑101 (MQ=255)


                                                                                                           ccg.gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGaaa      <  1:4576306/99‑1 (MQ=2)


                                                                                                             g.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGAAAcg    <  2:4736764/101‑2 (MQ=2)


CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCG.GGGGCGTGGATCGAAACTGC  >  NC_014034/1396868‑1397068

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG