Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,270 +G 3.0% coding (1152/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2701.→G 3.0% 10.7 ‑2.2 ‑0.1199coding (1152/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (102/91):  total (103/96)
Fisher's exact test strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.25e-01

Only 100 of 228 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTC  >  NC_014034/1397170‑1397370
                                                                                                     |                                                                                                         
gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                                                                            >  2:636134/1‑101 (MQ=255)
gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                                                                            >  2:4382313/1‑101 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc.g                                                                                                          >  2:3715565/1‑101 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACGCGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc.g                                                                                                          >  1:3341568/1‑101 (MQ=255)
          cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                                                                 >  2:3392251/1‑101 (MQ=255)
          cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGa                                                                                                 <  2:2715843/101‑1 (MQ=255)
          cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCC.GGCcgcgcgc                                                                                                 >  2:4235612/1‑100 (MQ=37)
           gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                                                                <  2:4573648/101‑1 (MQ=255)
           gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                                                                >  2:1733771/1‑101 (MQ=255)
            ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                                                               >  1:4314812/1‑101 (MQ=255)
              ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                                                             <  2:3232208/101‑1 (MQ=255)
              ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                                                             <  2:1333082/101‑1 (MQ=255)
                cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                          >  2:4170146/1‑101 (MQ=255)
                 gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.Gt                                                                                         >  1:2509317/1‑101 (MQ=255)
                  tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                                                                        >  2:1548726/1‑101 (MQ=255)
                   ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                      >  1:2314321/1‑101 (MQ=255)
                   ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                      >  1:1915049/1‑101 (MQ=255)
                    gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                     >  2:470730/1‑101 (MQ=255)
                     aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc                                                                                    >  2:3293997/1‑101 (MQ=255)
                       cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                 >  1:2729149/1‑101 (MQ=255)
                       cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                 >  1:598870/1‑101 (MQ=255)
                       cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                 >  1:1782861/1‑101 (MQ=255)
                        gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                >  1:2989686/1‑101 (MQ=255)
                         aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                               >  2:719221/1‑101 (MQ=255)
                         aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GCC.AGc.cccc                                                                               >  1:2404391/1‑100 (MQ=255)
                           aCGTGTCTGCCACGACGCCCTGCACTAGATACGCGCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGC.GCACGCGCGCACCGC.CTC.AGC.CCcgcg                                                                             >  2:3257089/1‑101 (MQ=37)
                             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                           >  1:1002314/1‑101 (MQ=255)
                             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                           >  1:3590343/1‑101 (MQ=255)
                             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                           >  1:2584528/1‑101 (MQ=255)
                              tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                        <  2:2115297/101‑1 (MQ=255)
                              tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                        <  2:4314565/101‑1 (MQ=255)
                                tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGt                                                                      <  1:636198/101‑1 (MQ=255)
                                 cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTc                                                                     >  2:1767154/1‑101 (MQ=255)
                                  taacACGAAGCCCTGCACCAGATCCGTGCCGTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGC.GCACGCGCGTAGCGC.GGC.AGT.CCCCCGAA..CGCCg                                                                    >  2:3901043/4‑101 (MQ=255)
                                  tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                    >  1:1557235/1‑101 (MQ=255)
                                   gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGc                                                                   >  2:2075211/1‑101 (MQ=255)
                                   gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGc                                                                   <  1:3975146/101‑1 (MQ=255)
                                     cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTc                                                                 <  2:2484602/101‑1 (MQ=255)
                                        gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccc                                                              >  2:3355915/1‑101 (MQ=255)
                                          cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                            <  1:2020211/101‑1 (MQ=255)
                                           gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCcg                                                           <  1:4369435/101‑1 (MQ=255)
                                               tGCACCAGATCCGCGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                       >  1:723438/1‑101 (MQ=255)
                                               tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                       <  2:2014887/101‑1 (MQ=255)
                                               tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACACCGC.GCACGCGCGCATGGC.GTA.AGC.CCCGCGAC..CGTCGCTTCCCCCGCggg                                                       >  2:2275070/1‑101 (MQ=37)
                                               cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                       <  2:4525893/100‑1 (MQ=255)
                                                   ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCg                                                   <  2:3050192/101‑1 (MQ=255)
                                                     ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                 <  2:2763590/91‑1 (MQ=2)
                                                     ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTg                                                 <  2:110177/91‑1 (MQ=2)
                                                     aGATCCGCGCCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTg                                                 >  1:4222772/1‑101 (MQ=255)
                                                      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                >  1:4522397/1‑101 (MQ=255)
                                                      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGCgg                                                >  2:3835662/1‑101 (MQ=255)
                                                        tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGAt                                              <  2:1458323/101‑1 (MQ=255)
                                                             gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGCACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                         <  1:4663260/101‑1 (MQ=255)
                                                              ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAG..CGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                          >  2:3690009/1‑101 (MQ=2)
                                                              ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                        <  2:771065/101‑1 (MQ=255)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaaag                                       >  2:1084881/1‑99 (MQ=255)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                       >  1:180432/1‑101 (MQ=255)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                       >  2:467856/1‑101 (MQ=255)
                                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                      <  1:1182131/101‑1 (MQ=255)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                   >  1:2166542/1‑101 (MQ=255)
                                                                    gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                  >  2:1974551/6‑101 (MQ=255)
                                                                    ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G...AAC.ACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                  <  2:1989096/99‑7 (MQ=0)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                  >  1:1058578/1‑101 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                 >  1:1536504/1‑101 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                <  1:2655/101‑1 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                              <  1:1341667/101‑1 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgccc                           >  2:4457308/1‑91 (MQ=0)
                                                                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                         <  2:2642265/101‑1 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                         >  1:3868288/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                        >  2:2207947/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                        >  2:2756886/1‑101 (MQ=255)
                                                                               ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                       <  1:73024/101‑1 (MQ=255)
                                                                               ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                       >  2:1901291/1‑101 (MQ=255)
                                                                                  gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                    <  2:4008288/101‑1 (MQ=255)
                                                                                  gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                    >  2:829068/1‑101 (MQ=255)
                                                                                  gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                    >  1:1584606/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACCCCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                  <  1:3117436/101‑1 (MQ=255)
                                                                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccccc                 >  2:1222747/1‑100 (MQ=255)
                                                                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                 >  2:3580776/1‑101 (MQ=255)
                                                                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                 >  1:1254118/1‑101 (MQ=255)
                                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                >  1:2014616/1‑101 (MQ=255)
                                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                <  1:885534/101‑1 (MQ=255)
                                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                >  1:2040226/1‑101 (MQ=255)
                                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                <  2:665116/101‑1 (MQ=255)
                                                                                       gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg               >  1:3946240/1‑101 (MQ=255)
                                                                                       gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg               >  2:4636233/1‑101 (MQ=255)
                                                                                        aTCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc              >  1:815715/1‑101 (MQ=255)
                                                                                         tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACAGCTTTACAGCCTTCGCGCCCCACGcc             <  1:3689841/101‑1 (MQ=255)
                                                                                          cGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa            <  2:186084/101‑1 (MQ=255)
                                                                                             aaCCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac         <  1:3489243/101‑1 (MQ=255)
                                                                                               cacCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg       <  1:3790313/101‑1 (MQ=255)
                                                                                               cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg       <  1:2915482/101‑1 (MQ=255)
                                                                                               cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg       >  2:3499953/1‑101 (MQ=255)
                                                                                               cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCT.CCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga      <  2:842165/101‑1 (MQ=255)
                                                                                                 ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac     >  1:987724/1‑101 (MQ=255)
                                                                                                  cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg    <  1:4145097/101‑1 (MQ=255)
                                                                                                   gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt   <  1:3415900/101‑1 (MQ=255)
                                                                                                   gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt   <  1:2561423/101‑1 (MQ=255)
                                                                                                    c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc  <  1:3294199/101‑1 (MQ=255)
                                                                                                    c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc  >  2:870618/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
GCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTC  >  NC_014034/1397170‑1397370

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG