Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,285 +T 3.2% coding (1167/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2851.→T 3.2% 16.0 ‑1.5 ‑0.0185coding (1167/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/5):  ref base (86/93):  total (87/98)
Fisher's exact test strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.30e-01

Only 100 of 201 total aligned reads displayed.

CGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGG  >  NC_014034/1397186‑1397385
                                                                                                     |                                                                                                        
cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                                         >  2:458698/1‑101 (MQ=255)
   ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                     >  1:2314321/1‑101 (MQ=255)
   ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                     >  1:1915049/1‑101 (MQ=255)
   ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAGCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.a                                                                                                     <  2:2576199/101‑1 (MQ=255)
    gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                                    >  2:470730/1‑101 (MQ=255)
      tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                                                                                 <  2:1506648/101‑1 (MQ=255)
      tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                                                                                 <  1:2037716/101‑1 (MQ=255)
       cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                                >  1:598870/1‑101 (MQ=255)
       cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                                >  1:1782861/1‑101 (MQ=255)
       cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                                >  1:2729149/1‑101 (MQ=255)
        gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                               >  2:2020094/1‑101 (MQ=255)
        gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                               >  1:2441289/1‑101 (MQ=255)
        gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                               <  1:297238/101‑1 (MQ=255)
         aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                              >  2:3430653/1‑101 (MQ=255)
           aCGTGTCTGCCACGCCGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                            <  1:1577406/101‑1 (MQ=255)
             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                          >  1:3590343/1‑101 (MQ=255)
             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                          >  1:2584528/1‑101 (MQ=255)
             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                          >  1:1595819/1‑101 (MQ=255)
             gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGaa                                                                                          >  1:1002314/1‑101 (MQ=255)
              tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                       <  2:2115297/101‑1 (MQ=255)
              tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..c                                                                                       <  2:4314565/101‑1 (MQ=255)
               gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..Cg                                                                                      >  2:1876408/1‑101 (MQ=255)
                tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGt                                                                                     >  2:1606953/1‑101 (MQ=255)
                tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGt                                                                                     <  1:636198/101‑1 (MQ=255)
                 cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTc                                                                                    >  2:1767154/1‑101 (MQ=255)
                  taacACGAAGCCCTGCACCAGATCCGTGCCGTCGCCTCCGCCGCGGAGGCGCTGCTCCCACGACCGC.GCACGCGCGTAGCGC.GGC.AGT.CCCCCGAA..CGCCg                                                                                   >  2:3901043/4‑101 (MQ=255)
                  tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCg                                                                                   >  1:4537357/1‑101 (MQ=255)
                   gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGc                                                                                  >  2:2075211/1‑101 (MQ=255)
                   gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGc                                                                                  <  1:3975146/101‑1 (MQ=255)
                    ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCt                                                                                 >  2:4212284/1‑101 (MQ=255)
                     cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTc                                                                                <  2:2696712/101‑1 (MQ=255)
                        gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccc                                                                             <  2:4381541/101‑1 (MQ=255)
                          cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                                           <  2:1916070/101‑1 (MQ=255)
                          cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTcccccc                                                                           <  1:305639/101‑1 (MQ=255)
                           gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCcg                                                                          <  1:4369435/101‑1 (MQ=255)
                              cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCgg                                                                       >  1:3309977/1‑101 (MQ=255)
                               tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                      <  1:217218/101‑1 (MQ=255)
                               tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                      <  2:824726/101‑1 (MQ=255)
                               tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACACCGC.GCACGCGCGCATGGC.GTA.AGC.CCCGCGAC..CGTCGCTTCCCCCGCggg                                                                      >  2:2275070/1‑101 (MQ=37)
                               cgCACCAGCTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCggg                                                                      <  2:4525893/100‑1 (MQ=255)
                                  aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGc                                                                   >  1:2517041/1‑101 (MQ=255)
                                   ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCg                                                                  <  2:3050192/101‑1 (MQ=255)
                                      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                               >  2:4144851/1‑101 (MQ=255)
                                      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTgg                                                               >  1:4522397/1‑101 (MQ=255)
                                      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGCgg                                                               >  2:3835662/1‑101 (MQ=255)
                                       aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGa                                                              <  2:2728995/101‑1 (MQ=255)
                                             gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGCACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                        <  1:4663260/101‑1 (MQ=255)
                                              ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGT.GCGCGCCATGAATTC.ATCGAGCGCCATCGAG..CGGCGCTCCCCCCGCGGGGGCGTGGCTCGaa                                                         >  2:3690009/1‑101 (MQ=2)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGaaaag                                                      >  2:1084881/1‑99 (MQ=255)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                      >  1:180432/1‑101 (MQ=255)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                      >  2:467856/1‑101 (MQ=255)
                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                     <  1:1182131/101‑1 (MQ=255)
                                                    gatcgcccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                 >  2:1974551/6‑101 (MQ=255)
                                                    ccccccccCGGGGGGCGTGGATCGAAACACCCC.GCACCTGTCGGCGGC.G...AAC.ACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatcgat                                                 <  2:1989096/99‑7 (MQ=0)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                 >  1:1058578/1‑101 (MQ=255)
                                                     cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                <  1:4216544/100‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                               <  1:2655/101‑1 (MQ=255)
                                                       cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                              <  1:3239325/101‑1 (MQ=255)
                                                        ccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                             >  2:3248165/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgccc                                          >  2:4457308/1‑91 (MQ=0)
                                                           cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..C..CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                          <  2:2416538/101‑11 (MQ=0)
                                                            gggggggCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.CGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                         <  1:3285559/99‑1 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                        >  1:3868288/1‑101 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                        >  2:3379979/1‑101 (MQ=255)
                                                              gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                       >  2:2207947/1‑101 (MQ=255)
                                                               ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                      <  1:73024/101‑1 (MQ=255)
                                                                gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                     <  1:905763/101‑1 (MQ=255)
                                                                 ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                    >  1:3944103/1‑101 (MQ=255)
                                                                  gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                   <  2:4008288/101‑1 (MQ=255)
                                                                   cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                  <  1:719294/101‑1 (MQ=255)
                                                                   cGTGGACCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                  <  2:3324356/101‑1 (MQ=255)
                                                                    gTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                 <  1:2800999/101‑1 (MQ=255)
                                                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccccc                                >  2:1222747/1‑100 (MQ=255)
                                                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                >  2:3580776/1‑101 (MQ=255)
                                                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                >  1:1254118/1‑101 (MQ=255)
                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                               <  1:4304761/101‑1 (MQ=255)
                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                               >  1:154644/1‑101 (MQ=255)
                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                               >  1:2014616/1‑101 (MQ=255)
                                                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                               <  2:665116/101‑1 (MQ=255)
                                                                       gATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                              >  2:4636233/1‑101 (MQ=255)
                                                                        aTCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                             >  1:815715/1‑101 (MQ=255)
                                                                         tCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACAGCTTTACAGCCTTCGCGCCCCACGcc                            <  1:3689841/101‑1 (MQ=255)
                                                                           gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                          >  2:853518/1‑101 (MQ=255)
                                                                           gAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                          <  1:1338806/101‑1 (MQ=255)
                                                                             aaCCCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                        <  1:3489243/101‑1 (MQ=255)
                                                                              acacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacg                       >  2:3279746/1‑101 (MQ=255)
                                                                               cacCGC.GCCCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                      <  1:3790313/101‑1 (MQ=255)
                                                                               cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                      <  2:730108/101‑1 (MQ=255)
                                                                               cacCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                      >  2:840500/1‑101 (MQ=255)
                                                                                acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                     <  1:2052398/101‑1 (MQ=255)
                                                                                 ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                    >  1:987724/1‑101 (MQ=255)
                                                                                  cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg                   <  1:4145097/101‑1 (MQ=255)
                                                                                   gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                  <  1:3415900/101‑1 (MQ=255)
                                                                                   gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                  <  1:2561423/101‑1 (MQ=255)
                                                                                    c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                 <  2:2757447/101‑1 (MQ=255)
                                                                                      gcACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                <  2:200671/101‑1 (MQ=255)
                                                                                        aCGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt              <  2:2836008/101‑1 (MQ=255)
                                                                                           cgcgAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc           >  1:1425434/1‑101 (MQ=255)
                                                                                                 cGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgcg     <  2:1360037/101‑1 (MQ=255)
                                                                                                    c.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg  <  2:1123752/101‑1 (MQ=255)
                                                                                                     |                                                                                                        
CGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAA..CGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGG  >  NC_014034/1397186‑1397385

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG