Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,674 +T 1.1% coding (1556/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6741.→T 1.1% 4.3 ‑2.2 ‑0.2261coding (1556/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (121/137):  total (124/137)
Fisher's exact test strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.78e-01

Only 100 of 274 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCC  >  NC_014034/1397574‑1397773
                                                                                                                                                                                                        
 ccccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTcc                                                                                                    <  2:4183909/101‑1 (MQ=255)
 ccccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTcc                                                                                                    <  2:2662750/101‑1 (MQ=255)
  cccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCg                                                                                                   <  2:3531934/101‑1 (MQ=255)
  cccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCg                                                                                                   <  2:2771885/101‑1 (MQ=255)
   ccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACTACAGAGTTCCgg                                                                                                  >  2:1176224/1‑101 (MQ=255)
   ccGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCgg                                                                                                  <  2:295176/101‑1 (MQ=255)
      cgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCt                                                                                               >  2:1589830/1‑101 (MQ=255)
      cgGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCt                                                                                               <  2:310467/101‑1 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCtt                                                                                              >  2:2218503/1‑101 (MQ=255)
       gggggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCtt                                                                                              >  1:2235847/1‑101 (MQ=255)
         gggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTCAGAGTTCCGGTCTtgt                                                                                            <  1:3684522/101‑1 (MQ=255)
         gggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgt                                                                                            >  2:1139911/1‑101 (MQ=255)
         gggCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgt                                                                                            <  1:4686831/101‑1 (MQ=255)
           gCGTGGATCGAACCATCCGCGGACCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttc                                                                                          <  2:4513396/101‑1 (MQ=255)
             gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctg                                                                                        <  1:978244/101‑1 (MQ=255)
             gTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctg                                                                                        <  2:551774/101‑1 (MQ=255)
              tGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgt                                                                                       >  1:1763782/1‑101 (MQ=255)
               ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgtt                                                                                      <  2:3658745/101‑1 (MQ=255)
               ggATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgtt                                                                                      <  2:1377920/101‑1 (MQ=255)
                gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgttc                                                                                     <  1:1734896/101‑1 (MQ=255)
                gATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTtgttctgttc                                                                                     >  1:1278179/1‑101 (MQ=255)
                 aTCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTcc                                                                                    <  1:1839244/101‑1 (MQ=255)
                 aTCGAAACACCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTcc                                                                                    <  2:2841624/101‑1 (MQ=255)
                  tCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCg                                                                                   <  2:1944394/101‑1 (MQ=255)
                  tCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCg                                                                                   <  2:1742583/101‑1 (MQ=255)
                  tCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCg                                                                                   <  1:2984279/101‑1 (MQ=255)
                   cGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCgg                                                                                  >  1:3790830/1‑101 (MQ=255)
                    gAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGt                                                                                 >  1:1104671/1‑101 (MQ=255)
                     aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTa                                                                                >  2:4118090/1‑101 (MQ=255)
                     aaaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTa                                                                                >  1:4330441/1‑101 (MQ=255)
                      aaCATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGACAGAGTTCCGGTCTTGTTCTGTTCCGGTaa                                                                               <  1:4205997/101‑1 (MQ=255)
                        cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                             <  1:387954/101‑1 (MQ=255)
                         aTCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAg                                                                            >  1:4046545/1‑101 (MQ=255)
                         aTCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAg                                                                            >  1:1907354/1‑101 (MQ=255)
                              cgGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                       <  1:6887/101‑1 (MQ=255)
                               ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                      >  2:3191031/1‑101 (MQ=255)
                                gATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTc                                                                     <  2:2929942/101‑1 (MQ=255)
                                gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTc                                                                     >  2:75926/1‑101 (MQ=255)
                                gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTc                                                                     <  1:305748/101‑1 (MQ=255)
                                  tCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccc                                                                   >  2:4623136/1‑101 (MQ=255)
                                   cGGCGCGACCCGCCCCGCCCATCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTcccc                                                                  <  2:3590124/101‑1 (MQ=255)
                                    ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                 >  1:3329528/1‑101 (MQ=255)
                                    ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                 >  1:761063/1‑101 (MQ=255)
                                    ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                 >  1:448505/1‑101 (MQ=255)
                                      cGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcg                                                               <  1:3392812/101‑1 (MQ=255)
                                      cGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcg                                                               >  1:3185105/1‑101 (MQ=255)
                                        tGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcgcg                                                             >  2:1850808/1‑101 (MQ=255)
                                         gACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCgg                                                            >  1:3422515/1‑101 (MQ=255)
                                         gACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCgg                                                            >  1:2367470/1‑101 (MQ=255)
                                         gACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGCCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCgg                                                            >  1:2874711/1‑101 (MQ=255)
                                            ccgccccgccCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCggggg                                                         <  1:2482098/101‑3 (MQ=255)
                                             cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGgggc                                                        <  1:1139997/101‑1 (MQ=255)
                                                cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                     >  1:1559392/1‑101 (MQ=255)
                                                cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                     <  1:3637541/101‑1 (MQ=255)
                                                 ccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTgg                                                    >  2:1264545/1‑101 (MQ=255)
                                                   gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  1:1996799/101‑1 (MQ=255)
                                                    cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  2:1713759/101‑1 (MQ=255)
                                                     ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  2:107414/1‑101 (MQ=255)
                                                      cATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               >  1:49946/1‑101 (MQ=255)
                                                         cGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGCGGCTCGAAAc                                            >  2:4279836/1‑101 (MQ=38)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTg                                         >  2:3782883/1‑101 (MQ=255)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGt                                        <  1:2292136/101‑1 (MQ=255)
                                                                cccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                     <  2:4413477/100‑1 (MQ=255)
                                                                 ccccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGa                                    >  2:335931/1‑101 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                  >  2:2743148/1‑101 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcgc                                 <  1:4300417/101‑1 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                               <  2:820044/101‑1 (MQ=255)
                                                                        cgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                             >  2:2109601/1‑101 (MQ=255)
                                                                         gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcc                            >  2:2066936/1‑100 (MQ=255)
                                                                         gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                            >  1:872372/1‑101 (MQ=255)
                                                                           gggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTATGACGCGCtcatc                          >  2:1048774/1‑101 (MQ=255)
                                                                             gCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAg                        <  2:4656914/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                       >  1:1628643/1‑101 (MQ=255)
                                                                              cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                       >  1:4181762/1‑101 (MQ=255)
                                                                              cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                       <  1:2743312/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                       <  2:1907242/101‑1 (MQ=255)
                                                                               gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                      <  2:4591562/101‑1 (MQ=255)
                                                                                tGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                     >  1:1448705/1‑101 (MQ=255)
                                                                                  gATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGt                   >  2:3712873/1‑101 (MQ=255)
                                                                                   aTCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc                  >  2:911010/1‑101 (MQ=255)
                                                                                   aTCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc                  >  2:3243567/1‑101 (MQ=255)
                                                                                    tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                 >  2:3275779/1‑101 (MQ=255)
                                                                                    tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                 >  2:406688/1‑101 (MQ=255)
                                                                                    tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                 <  2:4353290/101‑1 (MQ=255)
                                                                                    tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                 >  1:2107309/1‑101 (MQ=255)
                                                                                      gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg               >  2:3974064/1‑101 (MQ=255)
                                                                                      gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg               >  2:3028115/1‑101 (MQ=255)
                                                                                       aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa              >  1:1192813/1‑101 (MQ=255)
                                                                                        aaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt             >  2:868610/1‑101 (MQ=255)
                                                                                        aaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt             >  1:4389487/1‑101 (MQ=255)
                                                                                         aCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt            >  1:3482310/1‑101 (MQ=255)
                                                                                          cTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc           <  2:607612/101‑1 (MQ=255)
                                                                                           tACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa          >  2:2436736/1‑101 (MQ=255)
                                                                                           tACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCa          <  1:3995174/101‑1 (MQ=255)
                                                                                            aCAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAg         >  1:2639383/1‑101 (MQ=255)
                                                                                              agagTTCCGGTCTTGTTCTTTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc       >  1:1324559/1‑101 (MQ=255)
                                                                                              agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc       <  1:301570/101‑1 (MQ=255)
                                                                                                   tCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc  <  1:2929345/101‑1 (MQ=255)
                                                                                                   tCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc  <  2:2874535/101‑1 (MQ=255)
                                                                                                   tCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc  <  2:3465239/101‑1 (MQ=255)
                                                                                                                                                                                                        
CCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCC  >  NC_014034/1397574‑1397773

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG