Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,606,281 +T 1.5% coding (13/1083 nt) mraY → phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,606,2811.→T 1.5% 4.7 ‑2.9 ‑0.0198coding (13/1083 nt)mraYphospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (72/123):  total (75/123)
Fisher's exact test strand distribution p-value = 5.30e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.40e-01

Only 100 of 226 total aligned reads displayed.

CGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCT  >  NC_014034/1606181‑1606381
                                                                                                                                                                                                           
cGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGc                                                                                                        >  2:1731419/1‑101 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCt                                                                                                       <  2:652396/101‑1 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCt                                                                                                       >  2:3574523/1‑101 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCt                                                                                                       >  1:745979/1‑101 (MQ=255)
  aaaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCtt                                                                                                      >  1:1028081/1‑101 (MQ=255)
  aaaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCtt                                                                                                      >  1:3719502/1‑101 (MQ=255)
  aaaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCtt                                                                                                      >  1:2986042/1‑101 (MQ=255)
  aaaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGGCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCtt                                                                                                      >  1:3637576/1‑101 (MQ=255)
   aaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCttt                                                                                                     >  1:1051948/1‑100 (MQ=255)
    aGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGc                                                                                                    <  2:4484089/101‑1 (MQ=255)
    aGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGc                                                                                                    <  2:2565225/101‑1 (MQ=255)
     ggCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCg                                                                                                   <  2:3285237/101‑1 (MQ=255)
        tCGAAGGTTTCGCGCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAAc                                                                                                <  1:1469958/101‑1 (MQ=255)
         cGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCttttgtttc                                                                                               >  1:984847/1‑94 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCttttttacct                                                                                              >  1:3595211/1‑93 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCttttttacat                                                                                              >  1:399140/1‑93 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCttttctgacc                                                                                              >  1:2192319/1‑93 (MQ=255)
           aaGGTTTCGCTCGGGGTGGCCGCGCTAAG.AAACCCCGTCAGCCCCGGGCGGGAAGGGGCTTTAAAGGAAAAAAATAATGCTTTACTGGCTTGCGAACCttt                                                                                            <  1:3251543/101‑1 (MQ=255)
             ggTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTc                                                                                           <  2:2120589/101‑1 (MQ=255)
              gTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCg                                                                                          <  1:4412494/101‑1 (MQ=255)
              gTTGCGCTCGTCGTTGCCGCGCTCAGAAAGCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACGGGCTTGCGAACCTTTCg                                                                                          <  2:823997/101‑1 (MQ=255)
                  cGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.g                                                                                     <  1:1951780/101‑1 (MQ=255)
                   gCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.gg                                                                                    <  1:3563817/101‑1 (MQ=255)
                    cTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGGACCTTTTTGAC.ggg                                                                                   >  2:2491374/1‑101 (MQ=255)
                     tCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.gggg                                                                                  <  2:1168009/101‑1 (MQ=255)
                      cGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGAACATTTTTGAC.ggggg                                                                                 >  1:1695146/1‑101 (MQ=255)
                      cGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.ggggg                                                                                 <  2:2734467/101‑1 (MQ=255)
                      cGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.ggggg                                                                                 <  1:3418973/101‑1 (MQ=255)
                       gTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGc                                                                                <  2:433485/101‑1 (MQ=255)
                        tGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCg                                                                               <  2:4678656/101‑1 (MQ=255)
                         ggTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGa                                                                              <  1:4466281/101‑1 (MQ=255)
                          gTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGAt                                                                             <  2:1784496/101‑1 (MQ=255)
                          gTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGAt                                                                             <  2:635751/101‑1 (MQ=255)
                            tGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTTCACCTTTTGGTC.gggggggggtt                                                                           >  2:512784/1‑95 (MQ=255)
                            tGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGGACCTTTTTGGC.ggggggggcgt                                                                           >  2:251259/1‑95 (MQ=255)
                            tGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGAACCTTTCTGAC.gggggggggtt                                                                           >  2:3687252/1‑95 (MQ=255)
                             gACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTTAACCTTTTCGAC.gggggggggttt                                                                          >  2:522802/1‑94 (MQ=255)
                             gACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATttc                                                                          <  1:2630218/101‑1 (MQ=255)
                                 cgcTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCa                                                                      <  1:1731524/101‑1 (MQ=255)
                                 cgcTCAGAAAACTAGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACGGGCTTGCGAACCTTGCGGAC.GGGGGCGATTTCTTCa                                                                      <  1:1893283/101‑1 (MQ=255)
                                  gcTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTTAAACTTTTCGGC.GGGGGGGGTTTTTTCaa                                                                     >  2:1308171/1‑101 (MQ=255)
                                      aGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACctc                                                                 <  2:4033016/101‑1 (MQ=255)
                                      aGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACctc                                                                 <  1:2289231/101‑1 (MQ=255)
                                       gAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACctct                                                                <  2:3403409/101‑1 (MQ=255)
                                        aaaaCGCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCtt                                                               <  2:3178550/101‑1 (MQ=255)
                                           aCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGCACCTTTTTGAC.GGGGGGGGGTTCTTCTACCTCtttcc                                                            >  2:673557/1‑98 (MQ=255)
                                               ggTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTAc                                                        <  1:251284/101‑1 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                      <  2:2808263/101‑1 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                      <  2:1642721/101‑1 (MQ=255)
                                                   aGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCa                                                    <  2:2419437/101‑1 (MQ=255)
                                                   aGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCa                                                    <  1:3378641/101‑1 (MQ=255)
                                                    gCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCAc                                                   <  1:292934/101‑1 (MQ=255)
                                                      ccGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCt                                                 <  2:2706969/101‑1 (MQ=255)
                                                       cGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCtt                                                <  2:1817736/101‑1 (MQ=255)
                                                       cGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCtt                                                <  2:1600588/101‑1 (MQ=255)
                                                        ggCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTc                                               <  2:3719272/101‑1 (MQ=255)
                                                          cgcgGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTCGAAACCTTCGGGCGGGGGGGGGTTTTTTTAACC.CTTCCCCTCCATCACCTTccc                                             >  2:1497462/1‑100 (MQ=255)
                                                           gcgGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGGACCTTTTTGGC.GGGGGGGGTTTTTTTTACCTCTTCCCCCCCATCACCTTcccc                                            >  1:1431555/1‑99 (MQ=255)
                                                           gcgGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCcgc                                            <  1:2176038/101‑1 (MQ=255)
                                                              gCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTGGAAACTTTCCGCC.GGGGGGGGGTTTTTCAACCCCTTTCCCTACATCACCTTcccctcc                                         >  2:4566868/1‑96 (MQ=255)
                                                               cAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTCGAACCTTTCTGGC.GGGGGGGAGTTCTTCAACCTCTTTCTCTACATCACCCTCCGC.Gccc                                       >  1:886025/1‑100 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGAACCCTTTGGAC.GGGGGGGGTTTCTTTAACCTCTTCCCCCCCATCACCCTCCCC.Gccct                                      >  1:2770345/1‑99 (MQ=255)
                                                                 gagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTGAACCTTTTGGAC.GGGGGGGGTTTTTTTAACCTCTTCCCCCACATCACCCTccccccccgg                                      >  1:3791525/1‑93 (MQ=255)
                                                                  agGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTg                                    <  1:1203187/101‑1 (MQ=255)
                                                                      cTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTCGAACCTTTCGGGC.GGGGGGGGGTTTTTCAACCTCTTCCCCTCCATCACCCTCCCC.CCCCGTggggg                                >  1:3011250/1‑100 (MQ=255)
                                                                        tCAAGGGAAAAGAATAATGCTTTACTGGCTTTCGAACCCTTCGGGC.GGGGGGGGTTTTTTTAACCCCTTCCGCTCCATCACCCTCCGC.GCCCGGGGGGcgt                              >  1:1583980/1‑99 (MQ=255)
                                                                        tCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCct                              <  2:2185341/101‑1 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTTTGAACCTTTCGGGCGGGGGGGGGTTTTTTCAA.CTCTTTCCCCACATCCCCTTCCCCTCCCGGGGGGGCct                              >  1:4082624/1‑101 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTTCGGAACTTTTTGAC.GGGGGGGGGTTTTTCAACCTCTTTTCCTTCATCACCTTTCTC.GCCCGTGGGGCctt                             >  1:788364/1‑101 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTTCGAACCTTTTGGAC.GGGGGGGGTTTCTTCAACCTCTTCCCCCACATCACCTTCCGC.GCCGGGGGGGCctt                             >  1:585353/1‑101 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTTCGAACCTTTTCGGC.GGGGGGGGTTTCTTCTACCCCTTCCGCTACATCACCCTCCCC.GCCCGCGGGGGctt                             >  2:3274154/1‑101 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCctt                             <  2:2037118/101‑1 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCctt                             <  2:3813205/101‑1 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCctt                             <  1:512835/101‑1 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCctt                             <  2:1187866/101‑1 (MQ=255)
                                                                          aaGGGAAAAGAATAATGCTTTACTGGCTTTCGAACCTTTTGGGC.GGGGGGGGTTTTTTTAACCTCTTTCGCTACATCACCTTCCGC.GCCCGCGGGGGcttt                            >  2:3768137/1‑100 (MQ=255)
                                                                          aaGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCcttc                            <  1:431709/101‑1 (MQ=255)
                                                                          aaGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCcttc                            <  1:3866821/101‑1 (MQ=255)
                                                                            gggAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCcttctt                          <  1:424737/101‑1 (MQ=255)
                                                                               aaaaGAATAATGCTTTACTGGCTTTTGCAACCTTCGGAC.GGGGGGGATTTTTTTAACCCCTTCCGCGCCCTCACCCTCCCC.GCCCGCGGGGGCctcttccc                       >  2:2332778/1‑99 (MQ=255)
                                                                               aaaaGAATAATGCTTTACTGGCTTTCGAACCTTTTCGGC.GGGGGGGGGTTTTTTAAACTCTTCCGCTACCTCACCTTCCGC.GCCCGGGGGGGCCTCTTCAc                       >  1:1800194/1‑101 (MQ=255)
                                                                               aaaaGAATAATGCTTTACTGGCTTTCGAAACTTTCGGAA.GGGGGGGGTTTTTTTAACCCCTTCCGCTAAATCACCTTCCCC.GCCGGGGGGGCCTTCTTCAc                       >  1:1138201/1‑101 (MQ=255)
                                                                               aaaaGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCAc                       <  1:4125725/101‑1 (MQ=255)
                                                                                 aaGAATAATGCTTTACTGGCTTTCGAACCTTTCGGGC.GGGGGGGGTTTTTTCAACCTCTTCCGCTACATCACCTTCCGC.CCCCGGGGGGGCTTCTTCACcg                     >  1:3966921/1‑101 (MQ=255)
                                                                                   gAATAATGCTTTACTGGCTTTCGCACCCTTCGGGC.GGGGGGGGTTTCTTTCACACCTTCCCCTACCACACCTTCCCC.GCCGGTGGGGGCGTCTTTActccg                   >  2:1940303/1‑97 (MQ=255)
                                                                                     ataatGCTTTACTGGCTTTCGAACCTTTCGGAC.GGGGGCGGTTTTTTCAACCTCTTCCGCTACATCACCTTCCCC.GCCGGGGGGGCCCTCTTCCCCGCGCt                 >  2:2395340/1‑101 (MQ=255)
                                                                                       aatGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGa               <  2:4402693/101‑1 (MQ=255)
                                                                                        atGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGAt              <  2:4180750/101‑1 (MQ=255)
                                                                                        atGCTTTACGGGCTTGCCAACCTTTCGGAC.GGGGGCGATTTCTTCACCCTCTTCCGCTACATCACCTTCCGC.GCCAGTGGGGCCTTCTTCACCGCTCTGAt              <  2:1439292/101‑1 (MQ=255)
                                                                                         tGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGGTTTCTTTAACCTCTTCCGCTACATCACCTTCCCC.GCCGGGGGGGCCCTCTTCACCGCGCTGATc             >  2:1204155/1‑101 (MQ=255)
                                                                                          gCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGGGGTTTTTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCt            >  1:3743457/1‑101 (MQ=255)
                                                                                           cTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCtt           <  1:566053/101‑1 (MQ=255)
                                                                                            tttACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTc          <  2:2162798/101‑1 (MQ=255)
                                                                                            tttACTGGCTTGCGAACCTGTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTc          <  1:4748504/101‑1 (MQ=255)
                                                                                             ttACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCg         <  1:2591041/101‑1 (MQ=255)
                                                                                             ttACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCg         <  1:2492091/101‑1 (MQ=255)
                                                                                             ttACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCg         <  1:2369622/101‑1 (MQ=255)
                                                                                              tACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCgg        <  1:3517650/101‑1 (MQ=255)
                                                                                               aCTGGCTTGCGAACCCTTCGGGC.GGGGGCGATTTCTTTCACCTCTTCCCCTACATCACCTTCCGC.CCCGGGGGGGCCGTCTTTACCCCGCCGATCTTCCGc       >  2:2578776/1‑101 (MQ=255)
                                                                                                  ggCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTc    <  2:86791/101‑1 (MQ=255)
                                                                                                                                                                                                           
CGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTGCGAACCTTTCGGAC.GGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGC.GCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCT  >  NC_014034/1606181‑1606381

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG