Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,640,005 +A 95.8% intergenic (+85/‑60) mccB → / → mccA methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,640,0054.→A95.8% 2.5 ‑2.5 ‑0.671intergenic (+85/‑60)mccB/mccAmethylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha
Reads supporting (aligned to +/- strand):  new base (67/1):  ref base (2/1):  total (69/2)
Fisher's exact test strand distribution p-value = 8.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.80e-01

Only 100 of 108 total aligned reads displayed.

GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....G....G.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCC  >  NC_014034/1639905‑1640088
                                                                                                             |                                                                                                 
gATTTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g                                                                                                    <  2:3242383/101‑1 (MQ=255)
 aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGAATTTTGGGGAAAATGAAAACCAACCCCTTTATCTTTCCCCCAAAATACCCGGGGGGC.CC..ggg....g                                                                                                    >  1:1178971/1‑101 (MQ=255)
 aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....g                                                                                               <  1:2663759/101‑1 (MQ=255)
 aTCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....g                                                                                               <  2:2822602/101‑1 (MQ=255)
  tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGAATTTGGGGCAAGATTAAAACCATCCCTTTCATTTTGCCCCAAAAAA.CCCCGGGGGC.CC..ggg....g....gg                                                                                              >  1:599802/1‑100 (MQ=37)
  tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....gg                                                                                              <  1:1201147/101‑2 (MQ=255)
   cTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGGATTTGGGGGAAGATGAAAACCATCCCTTTCATCTTTCCCCCAATAT.TCCCGGGGGC.CC..ggg....g....ggg                                                                                             >  1:2059209/1‑99 (MQ=37)
   cTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....ggc                                                                                             <  1:2923644/101‑3 (MQ=255)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGGAAGATGAAACCCATCCCTTTTATCTTTCCCCCAAAAT.CCCCGGGGGG.CC..ggg....g....gggg                                                                                            >  1:2625853/1‑98 (MQ=25)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAACCCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....ggca                                                                                            <  1:751088/101‑4 (MQ=255)
    ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....ggca                                                                                            <  1:2486902/101‑4 (MQ=255)
     tCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....ggcag                                                                                           <  1:4533577/101‑5 (MQ=255)
      ccGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTTTGGGCAAGATGAAAACCATCCCTTTCATCTTTCCCCAAAAAT.TCCGGGGGGG.CC..ggg....g....gggggg                                                                                          >  2:2050754/1‑96 (MQ=25)
      ccGGATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGGAAAAAGAAACCCATCCCCTTCATCTTTCCCCCAAAAT.CCCCGGGGGC.CC..cgg....g....gggggc                                                                                          >  1:1222181/1‑96 (MQ=255)
      ccGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....ggcagc                                                                                          <  2:3084301/101‑6 (MQ=255)
       cGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTTTGGGGGAGATGAAAACCATCCCTTTCATCTTTCCCCAAATAA.CCCGGGGGGC.CC..ggg....g....gggggcg                                                                                         >  2:3607415/1‑95 (MQ=25)
       cGGATGTGACTTTCGATGCCTCCGGCGGGGCAATTTGGGGTATTTTAAACCCCCGCCTTTTCATTTTCTCCCAATTCT.CCCGTGGGCC.CG..ggg....g....ggacctc                                                                                         >  1:3832942/1‑95 (MQ=255)
       cGGATGTGACTTTCGATGCCTCCGGCGGGGAGATTTGGGGGAAAAAGAAAACCATCCCTTTCATTTTTCCCCCAAAAT.CCCCCGGGGG.CC..ggg....g....ggggggg                                                                                         >  1:1241367/1‑95 (MQ=255)
       cGGATGTGACTTTCGATGCCTCCGGCGGGGACATTTTGGGGCAGATTCTACCCATCCCCCTCAACTTCCCCCAAATAT.CCCCGGGGGC.GC..tgg....g....ggcactg                                                                                         >  2:3713257/1‑95 (MQ=255)
        ggATGTGACTTTCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATGAAACCCATCCCTTTCATCTTTCCCCAAATAT.CCCCGGGGGC.CC..ggg....g....ggcggcgc                                                                                        >  1:1365722/1‑94 (MQ=37)
        ggATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGGAAAAAGGAAAACACCCCTTTCTTCTTGCCCCCAATAT.CCCGGGGGGG.CC..GGG....G....g.....g...g...cggcc                                                                             >  2:2598487/1‑97 (MQ=255)
         gATGTGACTTTCGATGCCTCCGGCGGGGGTATTTGGGGGAAGATGAAAACCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..ggg....g....ggcggcgcc                                                                                       >  1:576630/1‑93 (MQ=25)
         gATGTGACTTTCGATGCCTCCGGCGGGGAGATTTTGGGCAAGGAGAAACCCCTCCCCTTCCTCTTTCCCCAAATAT.CCCCGGGGCC.CC..cgg....g....ggcatcgcc                                                                                       >  1:3848731/1‑93 (MQ=255)
          aTGTGACTTTCGATGCCTCCGGCGGGGGTATTTGGGGCAAGATGAAAACCCTCCCTTTCATCTTGCCCCACAAAT.CCCCGGGGGC.CC..ggg....g....ggcggcgccc                                                                                      >  1:733080/1‑92 (MQ=25)
            gtgACTTTCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATGAAACCCATCCCTTTCATTTTGCCCCAAATAT.TCCGCGGGGC.CC..GGG....G....g.....g...g...cgggccccc                                                                         >  2:3797483/1‑93 (MQ=255)
                    cGAGGCCGCCGGGGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CG..GGG....GGGCAG.....C...G...CCCCCCGCCACtt                                                                     <  2:2136944/101‑1 (MQ=18)
                      aTGCCTCCGGCGGGGATATTTGGGGCAAGATGAAAACCATCCCTTTCATCTTGCCCCCAATAT.CCCGGGGGGC.CC..GGG....GGGGAG.....C...G...CCCCCCGCCACTTcc                                                                   >  2:4691802/1‑101 (MQ=21)
                        ccgtcgggcggGGATTTTGGGGGCAAGATGACCCCCACCCCTTCTTTCTCCCCCCAAATCC.CCGGGGGGGC.CG..GGG....GGGGAG.....C...G...CCCCCCGCCACTTCCGc                                                                 <  2:2146254/95‑1 (MQ=255)
                          ccggggggggtATATTGGGGGAAAGAGAAACCCCACCCTTTTCATCTCCCCACAAATA..CCGGGGGGGC.CC..GGG....G....GGCC..C...GCC.CCCCCCGCCACTTCCGCCa                                                               <  2:4749304/94‑1 (MQ=255)
                                 ggggATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCa                                                        >  1:164117/1‑101 (MQ=25)
                                  gggATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAg                                                       >  1:3729482/1‑101 (MQ=25)
                                   ggATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAgc                                                      >  1:2805380/1‑101 (MQ=25)
                                     atatTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAgcgc                                                    >  2:316465/1‑101 (MQ=25)
                                     atatTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAgcgc                                                    >  2:410652/1‑101 (MQ=25)
                                     atatTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAgcgc                                                    >  2:4711333/1‑101 (MQ=21)
                                     atatTTGGGGCAAGATGAAACCCATCCCTTTCATCTTCCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAgcgc                                                    >  1:1615709/1‑101 (MQ=21)
                                       atTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGGGGGAG....G.....C...G...CCCCCCGACACTTCCGCCACGGAGCAGCAccc                                                  >  1:2298324/1‑101 (MQ=21)
                                        tttGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGcccc                                                 >  2:2529038/1‑101 (MQ=25)
                                        tttGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGcccc                                                 >  1:4421398/1‑101 (MQ=21)
                                         ttGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                                >  2:943005/1‑101 (MQ=25)
                                         ttGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                                >  1:3529178/1‑101 (MQ=25)
                                         ttGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg                                                >  1:687942/1‑101 (MQ=25)
                                          tGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg                                               >  2:3750556/1‑101 (MQ=25)
                                           ggggCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGc                                              >  2:1939009/1‑101 (MQ=25)
                                           ggggCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGc                                              >  1:3833556/1‑101 (MQ=25)
                                            gggCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGcc                                             >  1:1132578/1‑101 (MQ=25)
                                               cAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                          >  2:1881512/1‑101 (MQ=21)
                                               cAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt                                          >  2:3020307/1‑101 (MQ=25)
                                               cAAGATGAAACCCATCCCTTTCATCTTCCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACCGGCCCGCGCCCCGGCCgct                                          >  1:2875967/1‑99 (MQ=18)
                                                aaGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                         >  1:4139012/1‑101 (MQ=25)
                                                aaGATGAAACCCATCCCTTTAATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg                                         >  1:4513828/1‑101 (MQ=21)
                                                 aGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa                                        >  1:544546/1‑101 (MQ=21)
                                                 aGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa                                        >  2:4477221/1‑101 (MQ=25)
                                                   aTGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg                                      >  2:3404402/1‑101 (MQ=25)
                                                    aaaacccccccccTTTCCTCTTGCCAAAAATGC.CCGGGGGGGC.CG..GGG....G....GGCCCCC...C...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg                                      <  2:3287318/91‑1 (MQ=255)
                                                     gAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa                                    >  1:443048/1‑101 (MQ=25)
                                                     gAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa                                    >  2:2055668/1‑101 (MQ=25)
                                                      aaaCCCATCACTTTCATCTTACCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCAGCCACGGGGCAGCGCCCCGGCCGTTGACGAAg                                   >  2:445350/1‑101 (MQ=21)
                                                       aaCCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg                                  >  1:2547690/1‑101 (MQ=25)
                                                       aaCACATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg                                  >  1:3654816/1‑101 (MQ=21)
                                                         cccATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgag                                >  2:986686/1‑101 (MQ=25)
                                                         cccACCCTTTTCATC.TGCCCCAAATTG.CCCGGGGGGC.CCGGGGG....G....G.....C...GCCCCCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgag                                <  1:799167/101‑1 (MQ=255)
                                                          ccACCCCTTCCATCTGCCCCAAAATCT.CCGGGGGGGC.CG..GGG....G....GCCAC.C...C...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGgaga                               <  2:1581428/101‑1 (MQ=255)
                                                            aTCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc                             >  2:1288773/1‑101 (MQ=25)
                                                             tCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg                            >  1:4538908/1‑101 (MQ=25)
                                                             tCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg                            >  1:4732557/1‑101 (MQ=25)
                                                               ccTTTCATCTTGCCCCAAATAT.CCCGGGGGGCGCG..GGG....G....G.....CAGGG...CCCCCCGCCACTTCCGCCACGGGGCCGCGCCCCGGCCGTTGGCGAAGGGGACcgcg                          >  2:1487261/1‑101 (MQ=21)
                                                                 tttCATCTTGCCCCAAATAT.CCCGGGGGGC.TT..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt                        >  1:4226708/1‑101 (MQ=25)
                                                                  ttcctcctcccccAAATCC.CCGGGGGGGC.CCGGGGG....G....G.....C...GCCCCCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt                        <  2:2625701/92‑1 (MQ=255)
                                                                  ttCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATg                       >  2:3356416/1‑101 (MQ=25)
                                                                  ttCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGAGCCCCGGCCGTTGACGAAGGAGACCGCGATg                       >  1:4229187/1‑101 (MQ=21)
                                                                   tCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt                      >  2:4307558/1‑101 (MQ=25)
                                                                    cATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                     >  1:1547649/1‑101 (MQ=25)
                                                                    cATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                     >  1:361970/1‑101 (MQ=25)
                                                                    cATCTTGCCACAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt                     >  2:1355015/1‑101 (MQ=25)
                                                                      tCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTc                   >  1:1324923/1‑101 (MQ=25)
                                                                      tCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTc                   >  1:397874/1‑101 (MQ=25)
                                                                       cTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCa                  >  1:4457848/1‑101 (MQ=25)
                                                                       cTTGACCCAAATAT.CCCGGGGGGC.CCG.GGG....G....G.....CAGGG...CCCCCCCCCACTTCCCCCCCGGGGCAACGCCCCGGCCGCTGACGAAGGGGACCCCGAGGTTTCa                  >  2:4016567/1‑101 (MQ=12)
                                                                         tGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCaaa                >  2:1138210/1‑101 (MQ=25)
                                                                         tGCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCaaa                >  2:3239822/1‑101 (MQ=25)
                                                                          tccccAAATTC.CCCGGGGGGC.CC..GGG....G....GGC...C...GCCCCCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCaaa                <  1:816921/100‑1 (MQ=255)
                                                                          gCCCCAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCaaaa               >  1:3766772/1‑101 (MQ=25)
                                                                              cAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATc           >  2:1977851/1‑101 (MQ=25)
                                                                              cAAATAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATc           >  1:3555941/1‑101 (MQ=25)
                                                                               aaaTAT.CCCGGGGGGC.CC..GGG....G....GGCAA.C...G...CCCCCCGCCACTTCAGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGCCGGCGATGTTTCAAAAGAtac          >  1:540166/1‑99 (MQ=21)
                                                                                aaTAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCt         >  1:2726488/1‑101 (MQ=25)
                                                                                aaTAT.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCt         >  1:1443592/1‑101 (MQ=25)
                                                                                 atat.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg        >  2:3878987/1‑101 (MQ=37)
                                                                                 atat.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg        >  1:878589/1‑101 (MQ=37)
                                                                                  tat.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCCGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGa       >  2:4577645/1‑101 (MQ=25)
                                                                                  tat.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGa       >  1:2268471/1‑101 (MQ=37)
                                                                                   at.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCCCGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt      >  1:101088/1‑101 (MQ=25)
                                                                                   at.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt      >  2:1362994/1‑101 (MQ=37)
                                                                                   at.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt      >  1:2112544/1‑101 (MQ=37)
                                                                                    t.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCCCGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGCTGTTTCAAAAGATCCTGATc     >  1:941516/1‑101 (MQ=25)
                                                                                    t.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATc     >  2:2359065/1‑101 (MQ=37)
                                                                                    t.CCCGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATc     >  2:3410666/1‑101 (MQ=37)
                                                                                        cGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGcc  >  1:1466382/1‑101 (MQ=37)
                                                                                        cGGGGGGC.CC..GGG....GGGCAG.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGcc  >  1:658632/1‑101 (MQ=37)
                                                                                                             |                                                                                                 
GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGCAAGATGAAACCCATCCCTTTCATCTTGCCCCAAATAT.CCCGGGGGGC.CC..GGG....G....G.....C...G...CCCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCC  >  NC_014034/1639905‑1640088

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG