Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,841 +C 97.9% intergenic (‑94/+99) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8411.→C97.9% 4.0 ‑1.9 ‑0.2188intergenic (‑94/+99)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (68/115):  ref base (3/1):  total (72/116)
Fisher's exact test strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.13e-01

Only 100 of 223 total aligned reads displayed.

GTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCC  >  NC_014034/1734741‑1734936
                                                                                                     |                                                                                                  
gtgtCATCGAACCCTCCGGGGAATGGCGCATCCGAGCAGCCGGGGCGGGCAAGCGAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTg                                                                                                     <  2:2682636/101‑1 (MQ=255)
gtgtCATCGAACCCTCCGGGGAATGGCGCATCCAAGCCGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTg                                                                                                     <  1:4054636/101‑1 (MQ=255)
gtgtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTg                                                                                                     >  2:2929737/1‑101 (MQ=255)
 tgtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.c                                                                                                   <  1:4666521/101‑1 (MQ=255)
  gtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cc                                                                                                  >  2:3262196/1‑101 (MQ=255)
  gtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cc                                                                                                  <  1:3126152/101‑1 (MQ=255)
     aTCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc.c                                                                                              >  2:1490583/1‑101 (MQ=255)
       cGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc.ccg                                                                                            <  1:587735/101‑3 (MQ=255)
        gAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc.ccgt                                                                                           >  2:2442440/1‑98 (MQ=255)
        gAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc.ccgt                                                                                           >  2:3156700/1‑98 (MQ=255)
          aaCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.cccc.ccgtcc                                                                                         <  1:380602/101‑6 (MQ=255)
             cTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGc                                                                                       >  1:1064543/1‑101 (MQ=255)
               ccGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCgg                                                                                     >  1:4396240/1‑101 (MQ=255)
                cGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGa                                                                                    >  2:2867484/1‑101 (MQ=255)
                cGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGa                                                                                    >  1:1486780/1‑101 (MQ=255)
                 ggggAATGGCGCATCCTAGCGGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGAc                                                                                   <  2:65966/101‑1 (MQ=255)
                 ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGGGGAc                                                                                   >  2:1388138/1‑101 (MQ=255)
                 ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGACCCC.CGCCCTGCGGAc                                                                                   >  2:2221560/1‑101 (MQ=255)
                    gAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTcc                                                                                >  2:2016467/1‑101 (MQ=255)
                     aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTccc                                                                               >  1:567506/1‑101 (MQ=255)
                     aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTcca                                                                               >  2:3111257/1‑100 (MQ=255)
                      aTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGGCCTGCGGACTcccc                                                                              >  2:2563352/1‑101 (MQ=255)
                        ggCGCCTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTcccccc                                                                            <  1:2947813/101‑1 (MQ=255)
                        ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTcccccc                                                                            >  1:1433185/1‑101 (MQ=255)
                          cgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCgg                                                                          <  1:6639/101‑1 (MQ=255)
                           gtgtCCTAGCAGCCGGGNGGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCggg                                                                         <  2:652496/98‑1 (MQ=25)
                            ctgccTAGCGGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGa                                                                        <  2:1788962/98‑1 (MQ=25)
                            cagccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGa                                                                        <  2:789763/98‑1 (MQ=255)
                            cagccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGa                                                                        >  1:2713023/4‑101 (MQ=255)
                            cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTcccccttgga                                                                        <  2:4018125/101‑6 (MQ=255)
                            cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGa                                                                        >  2:441735/1‑101 (MQ=255)
                              cccgagcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGata                                                                      <  2:4082445/97‑1 (MQ=255)
                               ccgagcagcCGGGCCGGGCAAGTCAAAGGCTGACGCTGCGGCGGGCGGCGGGCTGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGTGatat                                                                     <  2:782021/98‑1 (MQ=18)
                                cTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATAtt                                                                    <  2:2384479/101‑1 (MQ=255)
                                cTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATAtt                                                                    <  2:4581953/101‑1 (MQ=255)
                                cTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATAtt                                                                    <  1:2614487/101‑1 (MQ=255)
                                 tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATAttt                                                                   >  1:1904133/1‑101 (MQ=255)
                                 tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATAttt                                                                   <  1:3102388/101‑1 (MQ=255)
                                 tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATAttt                                                                   >  2:3456399/1‑101 (MQ=255)
                                 tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCCGGATAttt                                                                   >  2:340977/1‑101 (MQ=255)
                                  agcggcCGGGGCGGCCAAGCCAAAGGCTGCCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTa                                                                  <  1:3746576/101‑1 (MQ=21)
                                  agcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTa                                                                  <  2:533267/101‑1 (MQ=255)
                                  agcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTa                                                                  >  2:435223/1‑101 (MQ=255)
                                   gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGAGATTTAt                                                                 >  1:765469/1‑101 (MQ=255)
                                    cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                <  1:4302540/101‑1 (MQ=255)
                                     agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                               >  1:2092070/1‑101 (MQ=255)
                                         ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                           <  1:227278/101‑1 (MQ=255)
                                          gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGAAATTTATGGCAAGa                                                          >  1:338895/1‑101 (MQ=255)
                                           gccgggcGAGCCAAAGGCTGAGGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGAt                                                         <  2:4518062/99‑1 (MQ=18)
                                            gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATg                                                        <  1:3647324/101‑1 (MQ=255)
                                             cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGa                                                       >  2:304788/1‑101 (MQ=255)
                                              gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGNCTCCCCCCGGGATATTTATGGCAAGATGaa                                                      >  1:2891139/1‑101 (MQ=255)
                                              gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaa                                                      <  2:1701869/101‑1 (MQ=255)
                                              gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaa                                                      >  2:512217/1‑101 (MQ=255)
                                               ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaaa                                                     <  2:2887412/101‑1 (MQ=255)
                                                gcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAc                                                    >  1:755631/1‑101 (MQ=255)
                                                gcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAc                                                    <  2:4169834/101‑1 (MQ=255)
                                                gcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAc                                                    <  2:4492492/101‑1 (MQ=255)
                                                 cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACt                                                   >  1:4740194/1‑101 (MQ=255)
                                                  aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                  >  2:1555744/1‑101 (MQ=255)
                                                    gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTgag                                                >  2:2287179/1‑99 (MQ=255)
                                                     ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                              >  1:412521/1‑101 (MQ=255)
                                                      cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                             >  1:2534045/1‑101 (MQ=255)
                                                       aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                            >  1:3886899/1‑101 (MQ=255)
                                                        aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                           <  1:4563168/101‑1 (MQ=255)
                                                          ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                         >  2:2922094/1‑101 (MQ=255)
                                                           gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                        <  1:884977/101‑1 (MQ=255)
                                                           gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                        <  1:4539795/101‑1 (MQ=255)
                                                           gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                        <  2:2580259/101‑1 (MQ=255)
                                                            cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGACATTTATGGCAAGATGAAGCTGC.GGCCTTCAt                                       >  2:737261/1‑101 (MQ=18)
                                                             tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                      >  1:4182281/1‑101 (MQ=255)
                                                              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                     <  2:906732/101‑1 (MQ=255)
                                                              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                     <  2:3077004/101‑1 (MQ=255)
                                                                 gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                  <  2:2766201/101‑1 (MQ=255)
                                                                   tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                >  1:1366074/1‑101 (MQ=255)
                                                                     cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                              <  1:1732641/101‑1 (MQ=255)
                                                                      gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                             <  1:3175813/101‑1 (MQ=255)
                                                                       ctaGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                            <  2:3777384/99‑1 (MQ=255)
                                                                        cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                           >  1:3111370/1‑101 (MQ=255)
                                                                        cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                           <  1:3546452/101‑1 (MQ=255)
                                                                        cAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCGCGCCCTGCGGAGTCCCCTCGGGGAATTTATGGCGAGATTAAACTGC.TGCCCTCATCTTGTCATAAat                           >  2:1866893/1‑101 (MQ=17)
                                                                         aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                          <  1:3466480/101‑1 (MQ=255)
                                                                         aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                          <  2:4130964/101‑1 (MQ=255)
                                                                          gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                         <  2:3371038/101‑1 (MQ=255)
                                                                           ccGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                        <  1:3014464/101‑1 (MQ=255)
                                                                            cGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       <  2:1204190/101‑1 (MQ=255)
                                                                            cGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       <  2:3878351/101‑1 (MQ=255)
                                                                            cGACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       >  2:214609/1‑101 (MQ=255)
                                                                             gACGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                      >  2:3241115/1‑101 (MQ=255)
                                                                              aCGGGCCGGGCAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                    <  1:3712903/101‑1 (MQ=255)
                                                                               cgggccgggcAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                   <  1:2856734/101‑1 (MQ=255)
                                                                                  gccgggcAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                <  1:3325771/101‑1 (MQ=255)
                                                                                    cgggcAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc              >  2:264076/1‑101 (MQ=255)
                                                                                    acggacggggggCTCTG.CCCC.CCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa             >  1:446867/7‑99 (MQ=2)
                                                                                     gggcAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg             <  1:1879611/101‑1 (MQ=255)
                                                                                      ggcAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgg            >  2:2243435/1‑101 (MQ=255)
                                                                                        cAGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg          >  2:3005833/1‑101 (MQ=255)
                                                                                         aGGGGGGCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTTTTGGAAGATGAAAATGG.GGGCCTCCTCTTGCCATAAATATCCC.GGGGTGCGGGGc         >  2:3998363/1‑101 (MQ=17)
                                                                                           gggggCGCTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag       <  1:3021677/101‑1 (MQ=255)
                                                                                                cgcTGCCCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.GGGGTGCGGGGCAGAGccc  <  1:2694640/101‑1 (MQ=255)
                                                                                                     |                                                                                                  
GTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCC.CGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCC  >  NC_014034/1734741‑1734936

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG