BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,734,917	+A	1.4%	intergenic (‑170/+23)	RCAP_rcc01603 ← / ← RCAP_rcc01604	hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,734,917	1	.→A	1.4%	4.2 ‑1.4 ‑0.1	221	intergenic (‑170/+23)	RCAP_rcc01603/RCAP_rcc01604	hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (3/0): ref base (111/107): total (114/107)
Fisher's exact test strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.19e-01

Only 100 of 244 total aligned reads displayed.


GACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCTCGGT  >  NC_014034/1734818‑1735017


 aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                                     <  1:3712903/101‑1 (MQ=255)


  cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTTC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                                                    >  2:3118619/1‑101 (MQ=255)


     tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                                                >  2:2435880/9‑101 (MQ=2)


     gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                                                 <  1:3325771/101‑1 (MQ=255)


       cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                                               >  2:264076/1‑101 (MQ=255)


       cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                                               <  2:3366969/101‑1 (MQ=255)


        gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                                              <  1:1879611/101‑1 (MQ=255)


         ggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgg                                                                                             >  2:2243435/1‑101 (MQ=255)


           cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATTGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                                                           >  1:410397/1‑101 (MQ=255)


            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                                          <  1:1230282/101‑1 (MQ=255)


            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                                          <  2:805109/101‑1 (MQ=255)


            aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                                          <  2:669064/101‑1 (MQ=255)


             ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGA.GGCCTTCATCTTCCCATCAATATACC.GGGGTGCGGGGCa                                                                                         >  2:2884096/1‑101 (MQ=17)


              gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                                        <  1:3021677/101‑1 (MQ=255)


                   cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.GGGGTGCGGGGCAGAGccc                                                                                   <  1:2694640/101‑1 (MQ=255)


                    gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                                  <  2:1473692/96‑1 (MQ=255)


                    gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                                  <  1:4483317/96‑1 (MQ=255)


                    gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                                                  >  2:925915/6‑101 (MQ=255)


                     ctgccccccGTCCTGCGGACTCCCCCCGGGGTATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                                 <  2:4680761/97‑1 (MQ=255)


                     ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                                 <  1:1725871/97‑1 (MQ=255)


                      tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                                                >  2:1449920/4‑101 (MQ=255)


                      tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                                                <  2:4587867/98‑1 (MQ=255)


                      tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                                                <  1:2929913/98‑1 (MQ=255)


                         cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                             <  1:4687206/101‑1 (MQ=255)


                         cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                             <  1:783874/101‑1 (MQ=255)


                         cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                             <  1:2016584/101‑1 (MQ=255)


                             cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.TGCCTTCATCTTGCCATAAATATCCA.GGGCTGCGGGGCAGAGCCCCGCTCAACCg                                                                         >  2:3041830/1‑101 (MQ=255)


                              gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                                        >  2:4443509/1‑101 (MQ=255)


                              gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                                        <  1:2442575/101‑1 (MQ=255)


                              gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                                        >  2:1778818/1‑101 (MQ=255)


                              gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                                        >  1:1549520/1‑101 (MQ=255)


                              gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                                        >  2:2155659/1‑101 (MQ=255)


                               tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                                       <  1:3563539/101‑1 (MQ=255)


                               tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                                       >  1:4734537/1‑101 (MQ=255)


                               tCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGaa                                                                       >  1:4313751/1‑101 (MQ=255)


                                 cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                                                     >  2:1185036/1‑101 (MQ=255)


                                 cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                                                     <  1:3998605/101‑1 (MQ=255)


                                    cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                                                  <  2:366449/101‑1 (MQ=255)


                                       aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                                               <  2:1064464/101‑1 (MQ=255)


                                          ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                            >  1:2162581/1‑101 (MQ=255)


                                          ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                            >  2:2759199/1‑101 (MQ=255)


                                           cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                           >  2:3886703/1‑101 (MQ=255)


                                            ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                                          <  2:4313504/101‑1 (MQ=255)


                                            ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                                          >  1:1748943/1‑101 (MQ=255)


                                              ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                                        >  2:3094009/1‑101 (MQ=255)


                                               cgtgATGTTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGACATAAATATCAC.GGGGTGCGGGGCAGAGCCCCGCTCACCCGAAGATGACAAGATCcgcg                                                       <  2:3038127/98‑1 (MQ=37)


                                                gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                                      <  2:4055458/101‑1 (MQ=255)


                                                  gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                                    >  1:459404/1‑101 (MQ=255)


                                                  gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                                    <  1:737335/101‑1 (MQ=255)


                                                   atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                                   <  2:1436680/101‑1 (MQ=255)


                                                   atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                                   <  2:1323321/101‑1 (MQ=255)


                                                      tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                                >  1:4560094/1‑101 (MQ=255)


                                                       ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                                               >  1:3297863/1‑101 (MQ=255)


                                                       ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                                               <  1:441779/101‑1 (MQ=255)


                                                        tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                              <  2:4714661/101‑1 (MQ=255)


                                                          tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                            >  2:3591985/1‑101 (MQ=255)


                                                          tGGCAAGATGAAACGGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                                            >  2:2933780/1‑101 (MQ=255)


                                                           ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                                           <  2:1904021/101‑1 (MQ=255)


                                                            gCAAGATGAAACTGC.GGCCTTCATCTTGCCATACTTAGCCC.GGGGTGCGGGGCAGAGTCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                                          <  1:4055852/101‑1 (MQ=38)


                                                            gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                                          <  1:1867005/101‑1 (MQ=255)


                                                            gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GCGGTGCGGGGCAGAGCCGCGCTGTACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                                          <  2:1527597/101‑1 (MQ=38)


                                                            gCAAAATGAACCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                                          <  1:914684/101‑1 (MQ=255)


                                                             cAAGATGAAACTGC.GGCCTTCATTTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCAGCGGACCGGCTTTCGCt                                         >  2:792754/1‑101 (MQ=37)


                                                             cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                                         <  2:3877766/101‑1 (MQ=255)


                                                              aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                                        >  1:2829535/1‑101 (MQ=255)


                                                               aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga                                       >  2:3187404/1‑101 (MQ=255)


                                                                 aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                                     >  2:3211997/1‑101 (MQ=255)


                                                                 aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg                                     >  2:483171/1‑101 (MQ=255)


                                                                   gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                                   <  2:4437074/101‑1 (MQ=255)


                                                                     aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                                 >  1:3191724/1‑101 (MQ=255)


                                                                     aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                                 <  1:4194478/101‑1 (MQ=255)


                                                                       cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg                               <  1:1464926/101‑1 (MQ=255)


                                                                        tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc                              >  1:100166/1‑101 (MQ=255)


                                                                             gCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGccc                          <  1:1490684/101‑1 (MQ=255)


                                                                                ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt                       >  2:3251717/1‑101 (MQ=255)


                                                                                ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt                       <  2:410353/101‑1 (MQ=255)


                                                                                ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt                       <  1:3006021/101‑1 (MQ=255)


                                                                                ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt                       >  2:2511138/1‑101 (MQ=255)


                                                                                 tCATCTTGCCATAAATATCCC.GGGGGGCGGGGAAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGACCGTTc                      >  1:213018/1‑101 (MQ=255)


                                                                                  cATCTTGCAATAAATATCCG.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCg                     <  2:4060372/101‑1 (MQ=255)


                                                                                   aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg                    <  2:477396/101‑1 (MQ=255)


                                                                                     cTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCa                  >  1:2282700/1‑101 (MQ=255)


                                                                                     cTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCa                  >  1:4209551/1‑101 (MQ=255)


                                                                                         ccATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc              >  1:3098752/1‑101 (MQ=255)


                                                                                         ccATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc              <  1:601580/101‑1 (MQ=255)


                                                                                            tAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtt           >  2:889294/1‑101 (MQ=255)


                                                                                             aaaTATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGttt          <  1:3456611/101‑1 (MQ=255)


                                                                                             aaaTATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGttt          >  1:961383/1‑101 (MQ=255)


                                                                                             aaaTATCCC.GGGGTGCGGGGCAGAGCCCCGCGCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGttt          <  1:3886945/101‑1 (MQ=255)


                                                                                              aaTATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtttt         >  1:3185512/1‑101 (MQ=255)


                                                                                              aaTATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGtttt         <  1:2884272/101‑1 (MQ=255)


                                                                                              aaTATCCC.GGGCTGCGGGGCAGAGCCCCGCTCAACCGAAGACGACAAGATCCGCGGCCCGGTTTTCGCTGATGACCAAGCGCCCGTTCGGCAGGACGtttt         >  2:3178720/1‑101 (MQ=38)


                                                                                               atatCCC.GGGGTGCGGGGCAGAGCCCCGCTGAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTc        <  1:311791/101‑1 (MQ=255)


                                                                                               atatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTc        <  2:2091949/101‑1 (MQ=255)


                                                                                               atatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGGCGTTTTc        >  2:691791/1‑101 (MQ=255)


                                                                                               atatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCGGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTc        >  1:4039183/1‑101 (MQ=255)


                                                                                                tatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTcc       >  2:1837393/1‑101 (MQ=255)


                                                                                                tatCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTcc       <  1:2943782/101‑1 (MQ=255)


                                                                                                   ccc.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGATTTAGCTGGTGAGCAAGCGCCCGTTCGGCACGCGGTTTTCCTCg    >  2:418593/1‑101 (MQ=38)


                                                                                                     c.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCTCGGt  <  1:4443759/101‑1 (MQ=255)


GACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCGTTTTCCTCGGT  >  NC_014034/1734818‑1735017

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG