Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,737,122 +A 1.9% intergenic (+63/‑228) RCAP_rcc01606 → / → RCAP_rcc01607 tRNA/rRNA methyltransferase, SpoU family/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,737,1221.→A 1.9% 4.6 ‑1.4 ‑1.6160intergenic (+63/‑228)RCAP_rcc01606/RCAP_rcc01607tRNA/rRNA methyltransferase, SpoU family/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (80/77):  total (83/77)
Fisher's exact test strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.70e-02

Only 100 of 194 total aligned reads displayed.

ACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACGGT  >  NC_014034/1737022‑1737222
                                                                                                                                                                                                         
aCGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAccgccg                                                                                                      <  2:4334797/101‑1 (MQ=255)
aCGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAccgccg                                                                                                      >  1:2609325/1‑101 (MQ=255)
  gggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCggg                                                                                                    >  2:2971471/1‑101 (MQ=255)
    gATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCggggg                                                                                                  >  1:1078173/1‑101 (MQ=255)
      tGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGcc                                                                                                >  1:3901405/1‑101 (MQ=255)
      tGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGcc                                                                                                <  1:1411111/101‑1 (MQ=255)
      tGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGcc                                                                                                >  2:2746909/1‑101 (MQ=255)
       gTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGccc                                                                                               >  2:1107165/1‑101 (MQ=255)
       gTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGccc                                                                                               >  1:2109424/1‑101 (MQ=255)
        ttGCGGCACCTTTTGCCCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGGCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCa                                                                                              <  2:3201238/101‑1 (MQ=255)
         tGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCaa                                                                                             >  1:2234922/1‑101 (MQ=255)
          gCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCaaa                                                                                            <  2:290376/101‑1 (MQ=255)
          gCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCaaa                                                                                            <  1:4460676/101‑1 (MQ=255)
           cGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAg                                                                                           <  1:3565092/101‑1 (MQ=255)
             gCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGc                                                                                         <  2:2920494/101‑1 (MQ=255)
             gCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGc                                                                                         <  1:4629525/101‑1 (MQ=255)
              cAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGcc                                                                                        >  1:2910891/1‑101 (MQ=255)
                gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCt                                                                                      <  1:3936954/101‑1 (MQ=255)
                 cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTc                                                                                     >  2:3975053/1‑101 (MQ=255)
                 cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTc                                                                                     >  1:875688/1‑101 (MQ=255)
                 cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTc                                                                                     >  2:2863246/1‑101 (MQ=255)
                  ttttGCGCCAGCGGGATTGAGGTCGACGGCTCTGACCGAGGCCATCGCCGAGGTCAAGCGCGAAATTGACCCCATGCACGCCGGGGCAACAAACGCCCTCg                                                                                    >  2:3856710/1‑101 (MQ=21)
                   tttGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCgg                                                                                   <  2:1863691/101‑1 (MQ=255)
                    ttGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGc                                                                                  <  1:89636/101‑1 (MQ=255)
                     tGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGcc                                                                                 <  2:2609173/101‑1 (MQ=255)
                       cgcCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAg                                                                               <  2:6736/101‑1 (MQ=255)
                        gcCAGCGTGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGGCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAgc                                                                              >  1:3304715/1‑101 (MQ=255)
                          cAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAgcgc                                                                            >  2:1312402/1‑101 (MQ=255)
                              gggATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGcgcccgc                                                                        <  2:871812/101‑1 (MQ=255)
                              gggATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGcgcccgc                                                                        >  1:3878556/1‑101 (MQ=255)
                               ggATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGcgcccgcc                                                                       <  2:3421700/101‑1 (MQ=255)
                               ggATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCACcgcccgcc                                                                       >  2:4265118/1‑101 (MQ=255)
                                 aTTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                                     <  2:2655983/101‑1 (MQ=255)
                                  ttGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGAGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccgccccg                                                                    >  2:2428050/1‑101 (MQ=255)
                                   tGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccgccccgc                                                                   >  2:514985/1‑101 (MQ=255)
                                    gAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCccgccccgcc                                                                  >  1:1787282/1‑101 (MQ=255)
                                    gAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCccgccccgcc                                                                  >  1:3946863/1‑101 (MQ=255)
                                      ggTCGAGGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                                <  2:3309763/101‑1 (MQ=255)
                                      ggTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                                <  1:3276319/101‑1 (MQ=255)
                                       gTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                               <  1:1314431/101‑1 (MQ=255)
                                         cGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATgg                                                             >  1:1935174/1‑101 (MQ=255)
                                          gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGGGCCCGCCCCGCCCATggc                                                            <  2:802365/101‑1 (MQ=255)
                                          gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCCTggc                                                            >  1:3462257/1‑101 (MQ=255)
                                          gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCCTggc                                                            >  1:1962940/1‑101 (MQ=255)
                                          gACGGATCTGACCGAGGCCATCGCCGAGGCCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                                            <  1:27293/101‑1 (MQ=255)
                                             ggATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcggg                                                         >  2:408129/1‑101 (MQ=255)
                                              gATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggg                                                        >  1:956760/1‑100 (MQ=255)
                                              gATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcgggc                                                        >  1:3757081/1‑101 (MQ=255)
                                                tCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCACGGCGGgcgc                                                      >  2:3777228/1‑101 (MQ=255)
                                                 cTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCt                                                     >  1:3607672/1‑101 (MQ=255)
                                                  tgcccGAGCCCCTCGCCGAGGTCACGCGCGATTTTGGCCCCATGCCCGCCGGGGGCCCAAAGGCCCTGGGCCAGCGCCCGCCCCGCCCATGGCCGGCGCtt                                                    <  2:975286/98‑1 (MQ=14)
                                                  tccccGAGCCCATCGCCGGGGTCACCCGCGACATTGGCCCCATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                                    <  1:1042193/98‑1 (MQ=14)
                                                  tGACCGAGGCCATCGCCGTGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                                    <  2:798542/101‑1 (MQ=255)
                                                  tGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                                    <  1:2396002/101‑1 (MQ=255)
                                                   gACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCtc                                                   >  1:1466810/1‑101 (MQ=255)
                                                    aCCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtct                                                  <  1:2781199/101‑1 (MQ=255)
                                                     ccGGGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctc                                                 <  1:3223985/101‑1 (MQ=255)
                                                      cGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcg                                                <  2:91397/101‑1 (MQ=255)
                                                       gagctcATCCCCGAGTAAACGCGCGCCATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgc                                               <  2:3096150/96‑1 (MQ=14)
                                                       gAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgc                                               <  2:1942390/101‑1 (MQ=255)
                                                       gAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgc                                               >  1:4159551/1‑101 (MQ=255)
                                                        aGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                              <  2:1029041/101‑1 (MQ=255)
                                                        aGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                              <  2:2109303/101‑1 (MQ=255)
                                                        aGGCCATCGCCGAGGCCCCGCGCGACATTGGCCCCAGGACCGCGGGGGGCCCAAGGGCCCTCGGCCAGCGCCCG.CCCGCCCATGGCGGGCGCTTCTcgccc                                             <  2:3304513/101‑1 (MQ=14)
                                                        aGGCCATCGCCGAGCTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                              <  2:1644396/101‑1 (MQ=255)
                                                           ccATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGGTTCTcgccccc                                           >  1:474626/1‑99 (MQ=255)
                                                            cATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcccgcc                                          <  2:1431735/101‑1 (MQ=255)
                                                             aTCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcggccgccc                                         >  2:4605011/1‑100 (MQ=255)
                                                             aTCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCccgccg                                         <  1:1280081/101‑1 (MQ=255)
                                                             aTCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCccgccg                                         >  1:1534297/1‑101 (MQ=255)
                                                              tCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGCCTTCTcgcccccccg                                        >  2:4115702/1‑99 (MQ=255)
                                                              tCGCCGAGCACATGCGCGACCTAGGCCCCACGACCCCCGGGGGCCCAAAGGCACTCGCCCGGCGCTGGCCCCGCCTATGGCGGGCGCTTCTCGCCCGCCgg                                        <  2:3625515/101‑1 (MQ=9)
                                                                 ccGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGc                                     <  2:3479975/101‑1 (MQ=255)
                                                                 ccGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGc                                     >  1:3575308/1‑101 (MQ=255)
                                                                 ccGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCGGGGc                                     >  1:1649974/1‑101 (MQ=255)
                                                                  tgAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCGCCCGCCgggggc                                    >  1:559150/2‑99 (MQ=255)
                                                                    aGGCCACGCGCGACATCAGCCCCATGACCGCCGAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCCTTCCCGCGCCgggggggg                                  >  2:1979158/1‑97 (MQ=17)
                                                                     ggTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGgc                                 <  2:3082528/101‑1 (MQ=255)
                                                                     ggTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGCAGgc                                 >  1:2761112/1‑101 (MQ=255)
                                                                      gTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCCGgcg                                >  1:4420224/1‑101 (MQ=255)
                                                                       tCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGgcgc                               <  2:3901163/101‑1 (MQ=255)
                                                                        cACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCCGGAGcc                              >  2:3166931/1‑101 (MQ=255)
                                                                          cgcgcgACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCgg                            <  2:62389/101‑1 (MQ=255)
                                                                            cgcgACATTGGCCCCATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGcc                          <  2:1197372/101‑1 (MQ=255)
                                                                               gACATTGGCCCCATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct                       <  1:3378022/101‑1 (MQ=255)
                                                                                   ttGGCCCCATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTg                   <  1:4382527/101‑1 (MQ=255)
                                                                                     ggCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCCCTGTTgcg                 >  1:215792/1‑101 (MQ=255)
                                                                                     ggCCCCAGGACCGCGGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg                 <  1:3082438/101‑1 (MQ=255)
                                                                                         ccATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTg             <  2:3052634/101‑1 (MQ=255)
                                                                                           aggcccgccgGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGAt           <  1:811622/97‑1 (MQ=255)
                                                                                           aTGCCCGCGGGGGCCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGAt           <  2:3452628/101‑1 (MQ=255)
                                                                                            tgcccgcggGGGCCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATc          <  2:446641/98‑1 (MQ=255)
                                                                                            tGACCGCCGGGGGCCAAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATc          <  2:1473796/101‑1 (MQ=255)
                                                                                             gACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCGGGGGCAGGCGCCGGCCCCCGTTGGGCTTGGTCg         >  1:4089468/1‑101 (MQ=14)
                                                                                              cccgccgGGGGCCAAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGa        <  2:1935057/100‑1 (MQ=255)
                                                                                                cggcgGGGCCCCAACGCCCCTCGGCCGGCGCCCGCCCCGCCCAGGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGaaa      <  1:4268162/98‑1 (MQ=17)
                                                                                                 gccgGGGGCCCAACGCCCCCCGGCCAGCCCCGGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAAc     <  2:1243985/101‑1 (MQ=17)
                                                                                                 gccgGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCCCCGTTGCGCTTGATCGGAAc     >  1:3499548/1‑101 (MQ=255)
                                                                                                   cgGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACgg   >  2:4050879/1‑101 (MQ=255)
                                                                                                    gggggCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCCTCTCCCCCGCCGGGGCCGGGGGCGGGCTCTGTTGCGCGTGATCGAAACggc  >  1:3144376/1‑100 (MQ=14)
                                                                                                                                                                                                         
ACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATCGAAACGGT  >  NC_014034/1737022‑1737222

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG