Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,769 +G 100% intergenic (+6/+129) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7691.→G100.0% 17.0 189intergenic (+6/+129)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (92/88):  ref base (1/7):  total (94/95)
Fisher's exact test strand distribution p-value = 6.46e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.27e-03

Only 100 of 224 total aligned reads displayed.

GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGG.CGCTGC.CCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCG  >  NC_014034/1740669‑1740863
                                                                                                      |                                                                                                   
gcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.Ga                                                                                                      <  1:1521672/101‑1 (MQ=255)
gcgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.Ga                                                                                                      >  1:1780939/1‑101 (MQ=255)
 cgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.g                                                                                                    >  1:2841302/1‑101 (MQ=255)
  gagaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gg                                                                                                   >  2:4135609/1‑101 (MQ=255)
   agaCGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.ggg                                                                                                  >  2:3035673/1‑101 (MQ=255)
     aCGATCGTGACCGCCGGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.ggggg                                                                                                <  2:1189690/101‑2 (MQ=255)
      cGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGTACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gggggc                                                                                               >  2:4302545/1‑99 (MQ=255)
      cGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gggggc                                                                                               <  1:2453890/101‑3 (MQ=255)
       gATCGTGCCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gggggcg                                                                                              <  2:1785187/101‑4 (MQ=255)
          cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTg                                                                                           >  1:436176/1‑101 (MQ=255)
           gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGc                                                                                          >  1:1680217/1‑101 (MQ=255)
            tGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.gggggcgcttcc                                                                                         <  2:3062769/101‑9 (MQ=255)
             gcccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGc.cc                                                                                       <  2:2695273/99‑1 (MQ=255)
                cGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCGGc.ccccg                                                                                    >  2:557254/1‑100 (MQ=255)
                   cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGc.ccccgccg                                                                                 <  2:1992394/101‑5 (MQ=255)
                   cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGc.ccccgccg                                                                                 >  1:672830/1‑97 (MQ=255)
                     gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAg                                                                              >  2:380391/1‑101 (MQ=255)
                          ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGAt                                                                         >  2:711241/1‑101 (MQ=255)
                             gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGcc                                                                      <  1:51182/101‑1 (MQ=255)
                              aGCCTACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCt                                                                     >  1:782753/1‑101 (MQ=255)
                                 cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCgg                                                                  >  1:718439/1‑101 (MQ=255)
                                 cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCgg                                                                  >  2:2484720/1‑101 (MQ=255)
                                     ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGcccc                                                              >  1:2939017/1‑101 (MQ=255)
                                     ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGcccc                                                              >  2:3870626/1‑101 (MQ=255)
                                      gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGccccc                                                             <  2:2413959/101‑1 (MQ=255)
                                      gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGccccc                                                             <  2:3723533/101‑1 (MQ=255)
                                       aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGcccccc                                                            <  2:3807801/101‑1 (MQ=255)
                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                           >  1:610410/1‑101 (MQ=255)
                                        agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGAGGCCTCGGCCCCCCg                                                           >  1:3055482/1‑101 (MQ=255)
                                         gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGGGATGCCTCGGCCCCCCgg                                                          >  1:4622825/1‑101 (MQ=255)
                                          acccGTTTTTGAACTATCTCGAAGGCAAAGCCCCCGCTATCTCCCGTCTTTGGGGGCGGG.GGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                         <  1:48073/99‑1 (MQ=255)
                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCTGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                         <  1:3994406/101‑1 (MQ=255)
                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                         <  2:2785332/101‑1 (MQ=255)
                                          aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                         <  2:322237/101‑1 (MQ=255)
                                            ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCgggga                                                       >  1:1657615/1‑99 (MQ=255)
                                            ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                       <  2:672599/101‑1 (MQ=255)
                                             cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGaga                                                      >  1:2118702/1‑99 (MQ=255)
                                              gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                     >  1:2000156/1‑101 (MQ=255)
                                              gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                     <  1:1026675/101‑1 (MQ=38)
                                              gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCAGC.CGAGGCGATGCCTCCGGCGCCCGGGatat                                                     >  2:4538940/1‑101 (MQ=255)
                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                    <  1:2390312/101‑1 (MQ=38)
                                               cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCCC.CGCGGCGATGCCTCGGCCCCCCGGGGTAtt                                                    >  1:1904322/1‑101 (MQ=255)
                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                   >  1:3240983/1‑101 (MQ=38)
                                                ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                   >  2:3408985/1‑101 (MQ=38)
                                                 tCTGGACTATCTCGAAGGCAAATACGCCGCGATCTACCGGCTTGGAGGGC.GAGGGGG.CGCCGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                  <  1:1271220/101‑1 (MQ=37)
                                                 tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                  <  2:2667028/101‑1 (MQ=38)
                                                  cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                 <  1:858361/101‑1 (MQ=38)
                                                   tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                >  2:2549455/1‑101 (MQ=38)
                                                   tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                >  2:1444392/1‑101 (MQ=38)
                                                    ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                               >  2:4147054/1‑101 (MQ=38)
                                                    ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                               >  2:2878993/1‑101 (MQ=38)
                                                    ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCCGC.CCCCCC.CCGGGGGGTGCCTCGGCCCCCCGGGGTATTTTGAg                                               >  1:4706226/1‑101 (MQ=255)
                                                     gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.c                                             <  1:3539383/101‑1 (MQ=38)
                                                      aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca                                            <  1:380435/101‑1 (MQ=38)
                                                      aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca                                            >  1:1052006/1‑101 (MQ=38)
                                                      aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca                                            >  2:494612/1‑101 (MQ=38)
                                                      aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca                                            >  2:4438094/1‑101 (MQ=38)
                                                       cTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca.a                                          <  1:2447453/101‑1 (MQ=37)
                                                       cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca.a                                          <  1:3359532/101‑1 (MQ=38)
                                                       cTATCCCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.Ca.a                                          <  1:2231273/101‑1 (MQ=37)
                                                        tATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTGGAGGGC.GAGGGGG.CCCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.Ag                                         <  1:4186306/101‑1 (MQ=25)
                                                        tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.Ag                                         >  1:141822/1‑101 (MQ=38)
                                                        tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.Ag                                         <  1:4130540/101‑1 (MQ=38)
                                                        tATCCCGAAAGCAAATACGCCCCGATCGACCGGCTTGGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.Ag                                         <  1:4561185/101‑1 (MQ=25)
                                                         aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGa                                        >  2:4466167/1‑101 (MQ=38)
                                                         aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGa                                        >  1:2742630/1‑101 (MQ=38)
                                                         aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGa                                        >  1:4500445/1‑101 (MQ=38)
                                                         aTCTCGAAAGCAAATACGCCGCGATCAACCCGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CTAGGCGATGCCCCGGCCCCCCGGGATATTTCGAG.CA.AGa                                        <  2:3678032/101‑1 (MQ=25)
                                                          tctcGAAAGCAAATACGCCCCGATCTCCCGGCTTTGAGGGC.GA.GGGGTCGCTCC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGAt                                       <  1:1549650/101‑1 (MQ=25)
                                                           ctcGAAANAAAATANGCAGNNATCTANNNNNTTTGAGGGC.GAGGGGG.CGCTGC.CCNNNC.CNNGGCGATGCCTCGGCCCCCCGGGATATTTNGAG.CA.AGATg                                      <  1:3201297/101‑1 (MQ=21)
                                                            tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGa                                     >  2:2828819/1‑101 (MQ=37)
                                                             cGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGaa                                    <  2:1638008/101‑1 (MQ=37)
                                                             cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGaa                                    >  1:313344/1‑101 (MQ=37)
                                                              gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGACGaaa                                   >  2:2189160/1‑101 (MQ=37)
                                                               aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAg                                  >  1:1938775/1‑101 (MQ=37)
                                                                aaGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAgg                                 <  2:3440426/101‑1 (MQ=37)
                                                                  gCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCa                               >  1:2502949/1‑101 (MQ=37)
                                                                   cAAATGCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTCC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCAt                              <  2:635306/101‑1 (MQ=37)
                                                                   cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCAt                              <  2:607735/101‑1 (MQ=38)
                                                                   cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCAt                              <  2:2938841/101‑1 (MQ=38)
                                                                    aaaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.GGGGGC.CCCCCC.CCCGGGGCGGGCCCGGCCCCCCCGGGTATTTTGAGACACAGATAAAAGGCa                               >  1:4746136/1‑101 (MQ=255)
                                                                     aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATcc                            >  1:3610008/1‑101 (MQ=37)
                                                                     aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATcc                            >  1:2603109/1‑101 (MQ=37)
                                                                        ccgccgcGATCTCCCGGCTTTGGGGGCGGG.GGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGt                         <  1:3035861/100‑1 (MQ=25)
                                                                         ccccgcTATCTCCCGGCTTTGAGGGC.GAGGGGG.CGTTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGtt                        <  2:2302926/99‑1 (MQ=25)
                                                                           ccgcGATCTACCGGCTTTGAGGGCGGG.GGGG.CGCGGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTc                      <  2:1926491/101‑1 (MQ=25)
                                                                             gcgATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCAt                    <  2:1284399/101‑1 (MQ=37)
                                                                               gATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCt                  >  1:2242387/1‑101 (MQ=37)
                                                                                aTCTACCGGCTTTGGGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCtt                 <  2:1858975/101‑1 (MQ=25)
                                                                                aTCTACCGGCTTTGAGGGCGGG.GGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCtt                 <  2:4622572/101‑1 (MQ=25)
                                                                                aTCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCtt                 >  1:2701105/1‑101 (MQ=25)
                                                                                  cTACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCGGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGAATCCGGTTTCATCTTGc               >  2:2914330/1‑101 (MQ=25)
                                                                                   tACCGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCCGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCt              >  2:2379400/1‑101 (MQ=25)
                                                                                    gacGGCTTGGGGGGC.GC.GGGG..GCGGCACCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCtt             <  1:4425157/99‑1 (MQ=18)
                                                                                     ccGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTc            <  1:4228622/101‑1 (MQ=25)
                                                                                      cGGCTTTGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTCa           <  1:624111/101‑1 (MQ=25)
                                                                                       ggCTTGGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTCaa          <  2:4745880/101‑1 (MQ=25)
                                                                                          tttGAGGGC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTCCTTCAAATa       >  2:3604247/1‑101 (MQ=25)
                                                                                           ttGAGGGC.GG.GGGGCCGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc      <  2:4705970/101‑1 (MQ=21)
                                                                                             gggggC.GAGGGGG.CGCTGC.CCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt    <  1:1254537/99‑1 (MQ=21)
                                                                                                      |                                                                                                   
GCGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGG.CGCTGC.CCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAG.CA.AGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCG  >  NC_014034/1740669‑1740863

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG