Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,785 +C 1.9% intergenic (+22/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7851.→C 1.9% 4.3 ‑2.0 ‑0.1211intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (93/114):  total (93/118)
Fisher's exact test strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.57e-01

Only 100 of 229 total aligned reads displayed.

GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGG.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880
                                                                                                   |                                                                                               
   ggtcggAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCg                                                                                          <  2:2014558/96‑1 (MQ=255)
    gTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGa                                                                                         >  1:3004275/1‑101 (MQ=255)
     ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGAt                                                                                        >  2:711241/1‑101 (MQ=255)
     ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGAt                                                                                        <  2:584103/101‑1 (MQ=255)
      tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATg                                                                                       >  1:2414094/1‑101 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  1:1936224/1‑101 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGcc                                                                                     <  1:51182/101‑1 (MQ=255)
          gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTc                                                                                   <  2:4140821/101‑1 (MQ=255)
           ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCg                                                                                  <  1:480101/101‑1 (MQ=255)
            cGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCgg                                                                                 >  1:718439/1‑101 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                <  1:4390572/101‑1 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                >  1:683697/1‑101 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                >  2:4430269/1‑101 (MQ=255)
              gcGGAAGAGCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGGGGGC.GAGGGGG.GGCTGCCCCCGC.CGAGGCGATGCCTCGGcc                                                                               <  2:1920696/100‑1 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              <  2:18282/101‑1 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              <  2:3798727/101‑1 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                             >  2:3870626/1‑101 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccc                                                                             >  1:2939017/1‑101 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  2:1126207/101‑1 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           <  2:3807801/101‑1 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  1:610410/1‑101 (MQ=255)
                   agaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCg                                                                          >  2:3621348/1‑101 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         <  1:1823949/101‑1 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GACGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         >  1:4700567/1‑101 (MQ=255)
                     acccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  1:3312487/99‑1 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCTGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  1:3994406/101‑1 (MQ=255)
                     aaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCggg                                                                        <  2:2785332/101‑1 (MQ=255)
                      aCCGCTTCTGGACTATCTCGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       <  1:4429229/101‑1 (MQ=255)
                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       <  2:2311088/101‑1 (MQ=255)
                       ccGCTTCTGGACTATCGCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                                      <  2:1739670/101‑1 (MQ=255)
                        cGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGaga                                                                     >  1:2118702/1‑99 (MQ=255)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    <  1:1026675/101‑1 (MQ=38)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    >  1:2000156/1‑101 (MQ=255)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCAGC.CGAGGCGATGCCTCCGGCGCCCGGGatat                                                                    >  2:4538940/1‑101 (MQ=255)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  2:3408985/1‑101 (MQ=38)
                           ttCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAttt                                                                  >  1:3240983/1‑101 (MQ=38)
                            tCTGGACTATCTCGAAGGCAAATACGCCGCGATCTACCGGCTTGGAGGGC.GAGGGGG.CGCCGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  1:1271220/101‑1 (MQ=37)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  1:3919479/101‑1 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 <  2:4663876/101‑1 (MQ=38)
                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               >  2:2549455/1‑101 (MQ=38)
                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               >  2:1444392/1‑101 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              >  2:2878993/1‑101 (MQ=38)
                                gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             >  1:1755174/1‑101 (MQ=38)
                                gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             <  1:3539383/101‑1 (MQ=38)
                                 aCTATCTCGAAAGCAAATCCGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            <  2:4252493/101‑1 (MQ=37)
                                 aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            >  1:1052006/1‑101 (MQ=38)
                                 aCTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            <  1:2469460/101‑1 (MQ=38)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           <  1:2447453/101‑1 (MQ=37)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           >  1:1467906/1‑101 (MQ=38)
                                  cTATCCCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           <  1:2231273/101‑1 (MQ=37)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  1:141822/1‑101 (MQ=38)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  1:3055213/1‑101 (MQ=38)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  1:2742630/1‑101 (MQ=38)
                                     tctcGAAAGCAAATACGCCCCGATCTCCCGGCTTTGAGGGC.GA.GGGGTCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGAt                                                        <  1:1549650/101‑1 (MQ=25)
                                      ctcGAAANAAAATANGCAGNNATCTANNNNNTTTGAGGGC.GAGGGGG.CGCTGCCCNNNC.CNNGGCGATGCCTCGGCCCCCCGGGATATTTNGAGCAAGATg                                                       <  1:3201297/101‑1 (MQ=21)
                                      ctcGAAAGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                                       <  1:4578013/101‑1 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGACGaaa                                                    >  2:2189160/1‑101 (MQ=37)
                                           aaGCAAATCCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                                  <  2:3440426/101‑1 (MQ=37)
                                              cAAATGCGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  2:635306/101‑1 (MQ=37)
                                              cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               <  2:607735/101‑1 (MQ=38)
                                              cAAATACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                                               >  1:1660740/1‑101 (MQ=37)
                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  1:3610008/1‑101 (MQ=37)
                                                aaTACGCCGCGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATcc                                             >  1:2603109/1‑101 (MQ=37)
                                                   aCGCCGCGATCTACCGGCTTTGAGGCC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGAGATTTCGAGCAAGATGAAAGGAATCCGGt                                          >  2:2827778/1‑101 (MQ=38)
                                                     gccgcGATCTCCCGGCTTTGGGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        <  1:840307/101‑1 (MQ=37)
                                                     gccgcGATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGttt                                        >  1:2259511/1‑101 (MQ=37)
                                                       cgcgATCTCCCGGCTTTGAGGGCGGG.GGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCa                                      <  2:2661432/101‑1 (MQ=25)
                                                        gcgATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCAt                                     <  2:1284399/101‑1 (MQ=37)
                                                         cgATCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATc                                    >  1:4545211/1‑101 (MQ=37)
                                                          gATCTCCCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCt                                   <  2:29259/101‑1 (MQ=25)
                                                           aTCTACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  >  1:2701105/1‑101 (MQ=25)
                                                             cTACCGGCTTTGAGGGCGGG.GGGG.CGCTGCCCCCGC.CGAGGCGATGCGTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                <  1:425420/101‑1 (MQ=25)
                                                              tACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt                               >  2:3040530/1‑101 (MQ=25)
                                                              tACCGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCCGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt                               >  2:2379400/1‑101 (MQ=25)
                                                                ccGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             >  2:2860494/1‑101 (MQ=25)
                                                                ccGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  1:4228622/101‑1 (MQ=25)
                                                                 cGGCTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCa                            <  1:624111/101‑1 (MQ=25)
                                                                  ggCTTTGAGGGC.GAGGGGG.CGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                           <  2:4463511/101‑1 (MQ=25)
                                                                  ggCTTGGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaa                           <  2:4745880/101‑1 (MQ=25)
                                                                   gCTTTGAGGGCGGG.GGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaaa                          <  1:4135855/101‑1 (MQ=25)
                                                                    cTTTGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         <  1:473445/101‑1 (MQ=25)
                                                                      ttGAGGGC.GG.GGGGCCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       <  2:4705970/101‑1 (MQ=21)
                                                                      ttGAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       >  2:2716995/1‑101 (MQ=25)
                                                                        gggggC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     <  1:1254537/99‑1 (MQ=21)
                                                                        gAGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     >  2:370618/1‑101 (MQ=25)
                                                                         aGGGC.GAGGGGG.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    <  1:4611654/101‑1 (MQ=25)
                                                                             gcgaggggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggg                 <  1:366552/96‑1 (MQ=255)
                                                                               gaggggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               >  2:3299973/4‑101 (MQ=255)
                                                                                 ggggg.CGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             <  2:672766/100‑1 (MQ=255)
                                                                                  gggg.CGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  2:313310/101‑1 (MQ=255)
                                                                                   ggg.CGCTGCCCCCGC.CGAGGCGATGCCTCGGTCCCTCGGGATATTTCGAGCAATATGAAAGGCATCCGGTTTCATCTTGCTTAAAATCCCTCGGGGGGAGt           <  2:782679/101‑1 (MQ=21)
                                                                                     g.cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         >  1:3066358/1‑101 (MQ=255)
                                                                                       cgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTccc        >  2:1976505/1‑101 (MQ=255)
                                                                                        gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  2:2435210/101‑1 (MQ=255)
                                                                                         cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc      <  2:1578114/101‑1 (MQ=255)
                                                                                         cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc      >  2:1361277/1‑101 (MQ=255)
                                                                                          tGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCACGCa     >  1:765387/1‑101 (MQ=255)
                                                                                           tccccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCa     <  2:3004087/100‑1 (MQ=255)
                                                                                              ccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  2:4500195/101‑1 (MQ=255)
                                                                                              ccccGCCGAGGCGATGCCGCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAggg  <  1:3437666/101‑1 (MQ=255)
                                                                                                   |                                                                                               
GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGGC.GA.GGGG.CGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG