Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,893 +C 97.9% intergenic (+130/+5) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,8931.→C97.9% 4.7 ‑2.1 ‑0.1188intergenic (+130/+5)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (121/63):  ref base (1/3):  total (122/66)
Fisher's exact test strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.34e-01

Only 100 of 221 total aligned reads displayed.

ATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.CCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAGGGGTCCCGC  >  NC_014034/1740793‑1740993
                                                                                                       |                                                                                                     
    cTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc                                                                                                   >  1:851591/1‑101 (MQ=255)
       ggCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGTCAG..CG.cccc.cct                                                                                               <  1:2579319/101‑3 (MQ=255)
       ggCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.cct                                                                                               <  2:4425780/101‑3 (MQ=255)
       ggCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.cct                                                                                               <  1:3621567/101‑3 (MQ=255)
       gccccccgGGGATTTTTCGGGCAAGATGAAAGGCATCCGGTTCAATCTGCCTTAAAAT.CCTGGGGGGGAATTCCGCAGGGACGGGGGGCAG..CG.cccc.ccta                                                                                              <  2:2728413/99‑4 (MQ=16)
        gCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGCGTCCCGCAGGGACGGGGGGCAG..CG.cccc.ccta                                                                                              >  2:4670423/1‑98 (MQ=255)
        gCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.ccta                                                                                              <  2:4205924/101‑4 (MQ=255)
         ccccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.cctac                                                                                             >  2:4751257/1‑97 (MQ=255)
         ccccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.cctac                                                                                             >  2:4509002/1‑97 (MQ=255)
          cccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.cctacg                                                                                            >  2:921759/1‑96 (MQ=255)
          cccccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.cccc.cctacg                                                                                            <  2:1760648/101‑6 (MQ=255)
             ccgaggtgtttCGAGGAAGGAGAAAAGGGTCCCGGTTCCTCTTTTTTTAAAAACCCTGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCa                                                                                          <  2:4557982/93‑1 (MQ=2)
             ccGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCa                                                                                          >  2:1665321/1‑101 (MQ=255)
               gggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCAtt                                                                                        >  1:2099444/1‑101 (MQ=255)
               gggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCAtt                                                                                        >  1:4518469/1‑101 (MQ=255)
               gggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCAtt                                                                                        <  2:3240782/101‑1 (MQ=255)
                ggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTg                                                                                       >  1:730910/1‑101 (MQ=255)
                ggATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTg                                                                                       >  1:3262960/1‑101 (MQ=255)
                  atatTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGAc                                                                                     >  1:3523155/1‑101 (MQ=255)
                     tttCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTg                                                                                  <  2:2700951/101‑1 (MQ=255)
                      ttCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGa                                                                                 >  2:3724066/1‑101 (MQ=255)
                       tCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGaa                                                                                >  2:81992/1‑101 (MQ=255)
                        cGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAg                                                                               <  1:921836/101‑1 (MQ=255)
                             aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGtt                                                                          >  2:744385/1‑101 (MQ=255)
                             aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGtt                                                                          >  1:2403567/1‑101 (MQ=255)
                             aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGtt                                                                          <  2:1938658/101‑1 (MQ=255)
                             aaGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGGGAAGCGGtc                                                                          >  2:1261029/1‑100 (MQ=255)
                                aTGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCAc                                                                       <  1:1195246/101‑1 (MQ=255)
                                aTGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCAc                                                                       <  1:4634860/101‑1 (MQ=255)
                                   aaaGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCTGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCgg                                                                    >  1:4016721/1‑101 (MQ=255)
                                    aaGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGc                                                                   >  1:527701/1‑101 (MQ=255)
                                     aGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCg                                                                  >  2:2410951/1‑101 (MQ=255)
                                     aGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCg                                                                  >  1:193489/1‑101 (MQ=255)
                                       gCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGtc                                                                >  1:1864150/1‑101 (MQ=255)
                                        cATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGtct                                                               >  1:3886020/1‑101 (MQ=255)
                                          tCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCg                                                             >  1:4724107/1‑101 (MQ=255)
                                             ggTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATc                                                          >  1:672745/1‑101 (MQ=255)
                                             ggTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATc                                                          <  2:1936107/101‑1 (MQ=255)
                                             ggTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATc                                                          >  1:3011001/1‑101 (MQ=255)
                                              gTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCa                                                         <  1:541027/101‑1 (MQ=255)
                                                ttCATCTTGCTACAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACg                                                       >  1:2006010/1‑101 (MQ=255)
                                                   aTCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCtt                                                    >  1:3670799/1‑101 (MQ=255)
                                                     cTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCtttt                                                  >  1:1161225/1‑101 (MQ=255)
                                                     cTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.CCCCTCTACGCCATTTACGTGAAGCGGCTCACCGCCGTCTCGATCACGCtttt                                                  >  2:3176924/1‑101 (MQ=25)
                                                       tGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTg                                                >  1:1605980/1‑101 (MQ=255)
                                                       tGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTg                                                >  1:3175715/1‑101 (MQ=255)
                                                           tCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcgg                                            >  1:1555448/1‑101 (MQ=255)
                                                           tCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcgg                                            >  2:2199027/1‑101 (MQ=255)
                                                             aaaTACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcggcg                                          >  2:1793469/1‑101 (MQ=255)
                                                              aaTACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGAcggcgg                                         >  2:1040693/1‑101 (MQ=255)
                                                               aTAGCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACggcggc                                        <  1:2914506/101‑1 (MQ=255)
                                                               aTACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACggcggc                                        >  1:4162425/1‑101 (MQ=255)
                                                               aTACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACggcggc                                        >  2:3109081/1‑101 (MQ=255)
                                                                tACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGgcggcg                                       >  2:1846047/1‑101 (MQ=255)
                                                                 aCCTGGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGt                                      <  2:3808141/101‑1 (MQ=255)
                                                                 aCCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGt                                      <  1:2860670/101‑1 (MQ=255)
                                                                  ccTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTc                                     <  1:3111679/101‑1 (MQ=255)
                                                                   cTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCt                                    >  1:3245119/1‑101 (MQ=255)
                                                                    tgggggggAGCCCCGCAGGGGCGGGGGGCAGC.CC.CCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTg                                   <  1:2511179/99‑1 (MQ=255)
                                                                    tCGGGGGGAGTCCCGCAGGGAGGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTg                                   <  2:396876/101‑1 (MQ=255)
                                                                    tCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTg                                   >  2:368454/1‑101 (MQ=255)
                                                                    tCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTg                                   >  2:2341103/1‑101 (MQ=255)
                                                                     cGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTgg                                  <  2:1542276/101‑1 (MQ=255)
                                                                      ggggggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTggg                                 >  1:2378491/1‑101 (MQ=255)
                                                                      ggggggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTggg                                 >  2:3557626/1‑101 (MQ=255)
                                                                      ggggggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTggg                                 >  1:563449/1‑101 (MQ=255)
                                                                      ggggggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTggg                                 >  1:3288639/1‑101 (MQ=255)
                                                                      ggggggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTggg                                 >  2:1753390/1‑101 (MQ=255)
                                                                       gggggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGc                                >  1:1253090/1‑101 (MQ=255)
                                                                          ggAGTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGaa                             >  2:2055062/1‑101 (MQ=255)
                                                                             gTCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAgcg                          <  2:2502805/101‑1 (MQ=255)
                                                                              tCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGAGGGGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGCCGGCGGGGTCTGGGCGAAgcgc                         >  2:4308288/1‑101 (MQ=37)
                                                                              tCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAgcgc                         >  1:4337237/1‑101 (MQ=255)
                                                                              tCCCGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAgcgc                         >  2:2998874/1‑101 (MQ=255)
                                                                               cccGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCt                        >  1:2687862/1‑101 (MQ=255)
                                                                               cccGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCt                        >  2:339111/1‑101 (MQ=255)
                                                                                ccggggggAGGGGGGGCGGC.CC.CCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTc                       <  1:4683762/96‑1 (MQ=255)
                                                                                ccGCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTc                       <  1:214680/101‑1 (MQ=255)
                                                                                  gCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCgg                     >  2:2850103/1‑101 (MQ=255)
                                                                                  gCAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCga                     >  1:1847955/1‑100 (MQ=255)
                                                                                  cccgggACGGGGGGCCGCCCC.CCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCg                      <  1:3724296/98‑1 (MQ=255)
                                                                                   cAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGc                    >  2:2981855/1‑101 (MQ=255)
                                                                                   cAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGc                    >  2:3985026/1‑101 (MQ=255)
                                                                                   cAGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTGCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGc                    >  2:4101744/1‑101 (MQ=255)
                                                                                    aGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACTGCGGCGTCTGGGCGAAGCGCTCGGcc                   >  1:1279800/1‑101 (MQ=255)
                                                                                    aGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGcc                   <  1:2057952/101‑1 (MQ=255)
                                                                                    aGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGcc                   <  2:4620957/101‑1 (MQ=255)
                                                                                    aGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGcc                   >  1:3754643/1‑101 (MQ=255)
                                                                                    aGGGACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGcc                   >  1:3983862/1‑101 (MQ=255)
                                                                                      ggACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAg                 >  2:876787/1‑101 (MQ=255)
                                                                                       gACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAgg                >  2:2662675/1‑101 (MQ=255)
                                                                                       gACGGGGGGCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAgg                >  2:2369764/1‑101 (MQ=255)
                                                                                         gggggggCCG..CCCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGt              <  1:1040772/100‑1 (MQ=255)
                                                                                          ggggggCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGGGCTCGGCCAGGGTc             <  1:2793995/101‑1 (MQ=255)
                                                                                          ggggggCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTc             <  2:2118581/101‑1 (MQ=255)
                                                                                           gggggCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCa            >  2:657731/1‑101 (MQ=255)
                                                                                           gggggCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCa            <  2:2602957/101‑1 (MQ=255)
                                                                                             gggCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAgg          >  2:4062051/1‑101 (MQ=255)
                                                                                               gCAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAgggg        <  1:2168240/101‑1 (MQ=255)
                                                                                                cAG..CGCCCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAGGGGt       >  2:3498168/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
ATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGGACGGGGGGCAG..CG.CCCC.CTACGCCATTGACGTGAAGCGGTTCACCGGCGTCTCGATCACGCTTTTTGACGGCGGCGTCTGGGCGAAGCGCTCGGCCAGGGTCAGGGGTCCCGC  >  NC_014034/1740793‑1740993

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG