Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,745,454 +C 100% intergenic (‑114/+654) RCAP_rcc01613 ← / ← metH4 hypothetical protein/methionine synthase subunit B

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,745,4541.→C100.0% 114.7 148intergenic (‑114/+654)RCAP_rcc01613/metH4hypothetical protein/methionine synthase subunit B
Reads supporting (aligned to +/- strand):  new base (74/40):  ref base (4/30):  total (78/70)
Fisher's exact test strand distribution p-value = 3.06e-08
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.15e-01

Only 100 of 189 total aligned reads displayed.

CAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACG.GCGCGAGCTTTGGCACCT.CA.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCG  >  NC_014034/1745355‑1745548
                                                                                                       |                                                                                               
cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c                                                                                                <  1:4368235/101‑1 (MQ=255)
   ccccgcGGTGCGGGCGGCGGGGGTCGGAAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.CCGCGCGCGAGC.TTGGCACCT.CA.c.ccc                                                                                            <  2:2649192/96‑1 (MQ=255)
    gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.cccc                                                                                           >  2:336816/1‑101 (MQ=255)
    gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.cccc                                                                                           <  2:611745/101‑1 (MQ=255)
    gcCCCGGTGCGGGGGGCGGGGATCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CC.c.cccc                                                                                           >  1:3497942/1‑101 (MQ=255)
      cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CC.c.cccccc                                                                                         <  2:2494599/101‑1 (MQ=255)
      cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.cccccc                                                                                         <  2:150498/101‑1 (MQ=255)
       ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccc                                                                                        <  1:1273138/101‑1 (MQ=255)
       ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccc                                                                                        >  2:1279294/1‑101 (MQ=255)
       ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccc                                                                                        <  2:3225637/101‑1 (MQ=255)
         ggTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccccg                                                                                      <  2:4180224/101‑3 (MQ=255)
          gTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccccgg                                                                                     <  2:345567/101‑4 (MQ=255)
          gTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccccgg                                                                                     <  1:4269687/101‑4 (MQ=255)
          gTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.ccccccccgg                                                                                     <  2:4119490/101‑4 (MQ=255)
          gTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.cccccccccg                                                                                     >  2:1603986/1‑98 (MQ=255)
           tGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CC.c.ccccccccggc                                                                                    <  2:1346917/101‑5 (MQ=255)
           tGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.c.cccccccccgc                                                                                    >  2:4531822/1‑97 (MQ=255)
            gcggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGCCCC.GCGCGAGC.TTGGCACCT.CA.c.ccccccccccgc                                                                                   >  2:1727366/1‑96 (MQ=255)
            gcggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CC.c.ccccccccggcg                                                                                   <  1:877875/101‑6 (MQ=255)
              ggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCa                                                                                  <  1:4233137/101‑1 (MQ=255)
               gggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGgcgccc                                                                                 >  2:3626062/1‑99 (MQ=255)
                 gggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCCccc                                                                               >  2:244908/1‑100 (MQ=255)
                  ggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCgg                                                                              <  1:863893/101‑1 (MQ=255)
                     gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGcac                                                                           <  2:728833/101‑1 (MQ=255)
                      ggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.C.CCCCCCCGGCGCACCGGCACAg                                                                         <  1:588154/101‑1 (MQ=255)
                       gggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAg                                                                         <  1:4742124/101‑1 (MQ=255)
                       gggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAg                                                                         <  2:3814355/101‑1 (MQ=255)
                        ggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGc                                                                        >  2:2273163/1‑101 (MQ=255)
                          tCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGAGACCT.CACC.CCCCCCCCGCGCCCCGGCACAGCCa                                                                      >  1:4072626/1‑101 (MQ=255)
                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCag                                                                     <  2:1272212/101‑1 (MQ=255)
                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCag                                                                     <  1:4677097/101‑1 (MQ=255)
                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCag                                                                     >  2:2294373/1‑101 (MQ=255)
                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCag                                                                     >  2:646386/1‑101 (MQ=255)
                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCag                                                                     <  1:3821544/101‑1 (MQ=255)
                           cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCCGCGCCCCCGCACAGCCag                                                                     >  1:4427527/1‑101 (MQ=255)
                            ggCAACCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCcga                                                                    >  2:4412197/1‑101 (MQ=255)
                             gcggCCCGGCCGTCGGACCGGCCGGCGCATTGTCGATCGCGGCGGTCG.CCGCGCGCGAGTTTGCCCCCCC.CC.C.CCCCCCCGGCGCACCGGCACAGCCagag                                                                   <  1:782772/98‑1 (MQ=255)
                                 cccGGCCGCCGGATCGGCCAGCGGCATGGCGATCGCGGCGTTCG.AGGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAgcg                                                               <  2:1760321/101‑1 (MQ=255)
                                  ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                              <  1:310765/101‑1 (MQ=255)
                                    ggCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGGCCCGGCGCAGCCagagcgagccg                                                            >  1:1820398/1‑94 (MQ=255)
                                       cGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCCCCGGCACAGCCAGAGAGCGcggcgg                                                         >  1:1189014/1‑101 (MQ=255)
                                       cGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGcggcgg                                                         >  2:2542000/1‑101 (MQ=255)
                                       cGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGcggcgg                                                         <  1:3889310/101‑1 (MQ=255)
                                         tCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGcc                                                       <  1:1348429/101‑1 (MQ=255)
                                         tCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGcc                                                       <  1:2866912/101‑1 (MQ=255)
                                          cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                      <  2:3605196/101‑1 (MQ=255)
                                          cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                      <  2:1073243/101‑1 (MQ=255)
                                            gATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                    <  1:3254477/101‑1 (MQ=255)
                                            gATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCCCACCGGCACCGCCCGAGAGCGCGGCCGCCagg                                                    >  1:835740/1‑100 (MQ=255)
                                               cGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                 <  2:2003461/101‑1 (MQ=255)
                                               cGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                 >  1:464406/1‑101 (MQ=255)
                                                ggCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGGGGCCAGagccc                                                >  2:3777997/1‑100 (MQ=255)
                                                 gCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                               <  1:3681303/101‑1 (MQ=255)
                                                 gCCAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                               >  2:4458954/1‑101 (MQ=255)
                                                   cAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccagcc                                             <  2:4285967/101‑1 (MQ=255)
                                                   cAGCGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.CGCCCCCCCGGCGCACCCGCACAGCCAGAGAGCCCGGCGGCCACagccagcc                                             >  2:4731033/1‑101 (MQ=255)
                                                      cGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                          <  1:908052/101‑1 (MQ=255)
                                                      cGGCATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCg                                          <  2:4052121/101‑1 (MQ=255)
                                                         cATGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCCCCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgagg                                       >  2:3000201/1‑100 (MQ=255)
                                                          aTGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                      >  1:3038628/1‑101 (MQ=255)
                                                          aTGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                      >  1:3516820/1‑101 (MQ=255)
                                                           tGTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAgg                                     <  1:3713669/101‑1 (MQ=255)
                                                            gTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAggg                                    <  1:1006477/101‑1 (MQ=255)
                                                            gTCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                   <  1:336850/101‑1 (MQ=255)
                                                             tCGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGc                                   <  2:3698170/101‑1 (MQ=255)
                                                              cGATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAAAGCCAGCCCCCAGGGGGcg                                  >  1:4660701/1‑100 (MQ=255)
                                                               gATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAg                                 >  1:1641693/1‑101 (MQ=255)
                                                               gATCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAg                                 >  2:3112977/1‑101 (MQ=255)
                                                                aTCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACTGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGt                                >  1:2543506/1‑101 (MQ=255)
                                                                 tCGCGGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTc                               <  2:2832951/101‑1 (MQ=255)
                                                                  cgcgGCGGTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                              <  1:2803479/101‑1 (MQ=255)
                                                                  cgcgGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                              <  1:646450/101‑1 (MQ=255)
                                                                  cgcgGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                              >  2:414560/1‑101 (MQ=255)
                                                                  cgcgGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGAGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                              >  2:796137/1‑101 (MQ=255)
                                                                  cgcgGCGGTCG.ACGCGCGCGAGC.TTGGCACCT.CA.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                             <  1:1054565/101‑1 (MQ=255)
                                                                   gcggcgGTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                             <  2:1188924/101‑1 (MQ=255)
                                                                    cggcggTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCCAGGGGGGAGGCAAg                            >  2:4502739/1‑101 (MQ=255)
                                                                     ggcggTCG.ACGCGCGCGAGC.TTGGCACCT.CA.C.CCCCTCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGa                          <  1:1969492/101‑1 (MQ=255)
                                                                       cggTCG.ACGCGCGCGAGC.TTGGCCCCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGaa                         <  2:1798967/101‑1 (MQ=255)
                                                                       cggTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGaa                         <  1:216963/101‑1 (MQ=255)
                                                                       cggTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGaa                         >  1:1786266/1‑101 (MQ=255)
                                                                        ggTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                        >  2:2361447/1‑101 (MQ=255)
                                                                        ggTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                        <  1:868150/101‑1 (MQ=255)
                                                                         gTCG.ACGCGCGCGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcg                       <  1:1258915/101‑1 (MQ=255)
                                                                         gTCG.ACGCGCGCGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcg                       >  2:1680374/1‑101 (MQ=255)
                                                                            cgacgcgcgcGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggc                     >  2:2137282/7‑101 (MQ=255)
                                                                             gacgcgcgcGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggcg                    <  1:4523340/96‑1 (MQ=255)
                                                                             gacgcgcgcGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggcg                    <  1:3000389/96‑1 (MQ=255)
                                                                             gacgcgcgcGAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGTGCCAGCCCCGAGAGGGCAGTCAAGGAAcggcg                    >  1:3835177/6‑101 (MQ=255)
                                                                                gcgcgcGAGC.TTGGCACCT.CA.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCggggc                >  2:979070/3‑99 (MQ=255)
                                                                                 cgcgcGAGC.TTGGCCCCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGcc                <  1:2646692/100‑1 (MQ=255)
                                                                                 cgcgcGAGC.TTGGCACCTCCC.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGcc                <  1:234752/100‑1 (MQ=255)
                                                                                   cgcgAGC.TTGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCg              >  1:809475/1‑101 (MQ=255)
                                                                                        agcttGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgc          <  2:3967057/98‑1 (MQ=255)
                                                                                         gcttGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCCAAggg         >  2:1654279/3‑99 (MQ=255)
                                                                                          cttGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGCAgcgc        >  2:2993444/2‑101 (MQ=255)
                                                                                           ttGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCGAGGGGCa       >  2:261515/1‑101 (MQ=255)
                                                                                           ttGGCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCGGAAgggcc       >  1:1112500/1‑100 (MQ=255)
                                                                                              gCACCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCAAACGCaaag    >  1:190247/1‑99 (MQ=255)
                                                                                                aCCT.CACC.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCGAAGCGCAAgggg  >  2:3437810/1‑99 (MQ=255)
                                                                                                       |                                                                                               
CAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCG.ACG.GCGCGAGCTTTGGCACCT.CA.C.CCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCG  >  NC_014034/1745355‑1745548

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG