Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,768,902 +G 97.7% intergenic (+29/‑27) lpxA → / → RCAP_rcc01636 acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,768,9021.→G97.7% 3.8 0.0 ‑1.1176intergenic (+29/‑27)lpxA/RCAP_rcc01636acyl‑ACP‑‑UDP‑N‑acetylglucosamine O‑acyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (87/85):  ref base (2/2):  total (89/87)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.32e-02

Only 100 of 202 total aligned reads displayed.

AGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.GG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGCT  >  NC_014034/1768802‑1768996
                                                                                                       |                                                                                                
agCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.Gc.c                                                                                                   <  1:816122/101‑1 (MQ=255)
 gccgccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.g                                                                                                 >  2:3979983/1‑101 (MQ=255)
   cgccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.g                                                                                              >  1:3823722/1‑101 (MQ=255)
   cgccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.g                                                                                              >  1:2110123/1‑101 (MQ=255)
     ccACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg                                                                                            <  1:4178504/101‑1 (MQ=255)
        cGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.ggg                                                                                        >  2:1529558/1‑100 (MQ=255)
         gTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.gggc                                                                                       >  2:4204514/1‑99 (MQ=255)
          tCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.gggcg                                                                                      <  2:3336400/101‑4 (MQ=255)
          tCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.gggcg                                                                                      >  1:4543430/1‑98 (MQ=255)
           ccGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.gggcgc                                                                                     <  1:106229/101‑5 (MQ=255)
           ccGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.gggcgc                                                                                     >  1:438353/1‑97 (MQ=255)
              cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCt                                                                                   >  2:3114390/1‑101 (MQ=255)
              cgAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.gg.ggg.gggcgccct                                                                                  >  2:1995300/1‑94 (MQ=255)
               gAAGTGCTTTATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCTCTGATC.GC.CGGG.GGG.GGCGCGCTg                                                                                  <  2:3047480/101‑1 (MQ=255)
               gAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTg                                                                                  >  2:4387732/1‑101 (MQ=255)
               gAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTg                                                                                  <  1:2394008/101‑1 (MQ=255)
               gAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.GG.GGGCGGCGCGCTg                                                                                  >  2:185795/1‑101 (MQ=255)
                aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGc                                                                                 >  1:4339897/1‑101 (MQ=255)
                aaGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGc                                                                                 >  1:1908989/1‑101 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGcc                                                                                <  2:3720279/101‑1 (MQ=255)
                 aGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGcc                                                                                <  1:590554/101‑1 (MQ=255)
                  gTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCg                                                                               >  2:4036704/1‑101 (MQ=255)
                    gCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCggg                                                                             <  1:2795793/101‑1 (MQ=255)
                    gCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCggg                                                                             >  2:3579165/1‑101 (MQ=255)
                     cTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCgggg                                                                            <  2:2122324/101‑1 (MQ=255)
                     cTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCgggg                                                                            >  2:4062756/1‑101 (MQ=255)
                      ttGATTTCATTCTGGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGgc                                                                           <  2:1769309/101‑1 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGgctg                                                                         >  1:836708/1‑101 (MQ=255)
                        gATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGgctg                                                                         <  1:3034784/101‑1 (MQ=255)
                          tttCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGgctgct                                                                       <  2:739051/101‑1 (MQ=255)
                            tCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCttt                                                                     <  2:2110002/101‑1 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCtttt                                                                    <  2:4216871/101‑1 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCtttt                                                                    <  2:3838032/101‑1 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCtttt                                                                    >  1:2722823/1‑101 (MQ=255)
                             cATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCtttt                                                                    <  1:2994878/101‑1 (MQ=255)
                             cATTCTTGGTCCCGCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCGGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCtttt                                                                    <  1:1754875/101‑1 (MQ=255)
                              aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGTCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTg                                                                   <  1:4036946/101‑1 (MQ=255)
                              aTTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTg                                                                   >  2:65060/1‑101 (MQ=255)
                                  ttGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAgg                                                               >  2:1894659/1‑101 (MQ=255)
                                    ggTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGcc                                                             >  1:1251519/1‑101 (MQ=255)
                                     gTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCcg                                                            <  2:762457/101‑1 (MQ=255)
                                     gTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCcg                                                            <  1:2336420/101‑1 (MQ=255)
                                     gTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCcg                                                            <  1:97975/101‑1 (MQ=255)
                                       cccTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCcgcg                                                          >  1:1010500/1‑101 (MQ=255)
                                        ccTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCgcgc                                                         >  1:1528278/1‑101 (MQ=255)
                                           cGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCgg                                                      <  2:3858368/101‑1 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                     >  1:3194132/1‑101 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                     <  2:413088/101‑1 (MQ=255)
                                            ggACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGa                                                     >  2:1624017/1‑101 (MQ=255)
                                                cGCGGCTTCCCGACGCCGCGATGAGCGCGGGATCGGAAACCGGGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                 <  1:2177196/101‑1 (MQ=255)
                                                cGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATg                                                 >  1:3526079/1‑101 (MQ=255)
                                                  catcgtCCTGACGCCGCGTTGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCt                                               <  2:1065711/96‑1 (MQ=255)
                                                     cTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGt                                            >  1:3212388/1‑101 (MQ=255)
                                                      ttCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTc                                           >  1:3288238/1‑101 (MQ=255)
                                                      ttCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTc                                           <  2:4570743/101‑1 (MQ=255)
                                                        ccTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTg                                         <  1:3579384/101‑1 (MQ=255)
                                                        ccTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTg                                         <  2:3193931/101‑1 (MQ=255)
                                                        ccTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTg                                         <  1:4134699/101‑1 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAATCCGCGTGGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                        <  2:2930777/101‑1 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                        >  1:2788051/1‑101 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                        <  2:2220862/101‑1 (MQ=255)
                                                         cTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgc                                        >  2:1249729/1‑101 (MQ=255)
                                                          tGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcg                                       >  2:21373/1‑101 (MQ=255)
                                                           gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                      <  2:1975478/101‑1 (MQ=255)
                                                           gACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTgcgc                                      <  2:3008121/101‑1 (MQ=255)
                                                            aCGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGttctggcg                                     <  2:4497314/101‑8 (MQ=255)
                                                                cgcgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTgg                                 <  1:2367528/101‑1 (MQ=255)
                                                                 gcgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGa                                <  1:130103/101‑1 (MQ=255)
                                                                 gcgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGa                                <  1:137788/101‑1 (MQ=255)
                                                                  ggaggAGCGCGGGATCTGAAACCGCGCTGAGCGGC.G.GG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                               <  1:4397759/97‑1 (MQ=255)
                                                                  cgATGAGCGCGGGATCTGAAACCGCGCTGGTC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                               <  1:1731284/101‑1 (MQ=255)
                                                                  cgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                               <  2:2707428/101‑1 (MQ=255)
                                                                  cgATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGaa                               <  1:1436743/101‑1 (MQ=255)
                                                                   gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAg                              >  2:4642621/1‑101 (MQ=255)
                                                                   gATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAg                              <  1:4204760/101‑1 (MQ=255)
                                                                    aTGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAgg                             >  1:2930429/1‑101 (MQ=255)
                                                                     tGAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGt                            >  2:2850522/1‑101 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                           >  2:1476143/1‑101 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                           <  2:1639725/101‑1 (MQ=255)
                                                                      gAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.GGTGGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTc                           <  1:4102573/101‑1 (MQ=255)
                                                                       aGCGCGGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCa                          <  2:3451565/101‑1 (MQ=255)
                                                                         cgcgGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTTCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGc                        <  2:3029287/101‑1 (MQ=255)
                                                                           cgGGATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGccc                      <  2:918472/101‑1 (MQ=255)
                                                                             ggATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCCGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTGAGCCcgc                    >  2:388489/1‑101 (MQ=255)
                                                                              gATCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCcgcg                   <  1:1641296/101‑1 (MQ=255)
                                                                                tCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGcc                 >  1:4640169/1‑101 (MQ=255)
                                                                                tCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGcc                 >  2:415552/1‑101 (MQ=255)
                                                                                tCTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGcc                 >  2:2939528/1‑101 (MQ=255)
                                                                                 cTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGGGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCaagatcggaaa                >  1:2903096/1‑91 (MQ=255)
                                                                                 cTGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCg                >  2:1841374/1‑101 (MQ=255)
                                                                                  tGAAACCGCGCTGATC.GC.CGGG.GGG.GGGGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCgg               >  1:3676259/1‑101 (MQ=255)
                                                                                  tGAAACCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCgg               >  1:3956289/1‑101 (MQ=255)
                                                                                    aaaCCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGc             >  2:241907/1‑101 (MQ=255)
                                                                                      aCCGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCgg           <  2:1425656/101‑1 (MQ=255)
                                                                                       ccGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGa          <  2:2605525/101‑1 (MQ=255)
                                                                                       ccGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGa          >  2:1248659/1‑101 (MQ=255)
                                                                                       ccGCGCTGATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGa          >  2:2763631/1‑101 (MQ=255)
                                                                                         gcgcTGATC.GCGG.GG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATc        <  1:2671448/101‑1 (MQ=255)
                                                                                              gATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGtgc   <  1:2299755/101‑1 (MQ=255)
                                                                                              gATC.GC.CGGG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAAAGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGCTCGGtcc   >  2:2195790/1‑99 (MQ=255)
                                                                                                       |                                                                                                
AGCCGCCACGTCCGCGAAGTGCTTGATTTCATTCTTGGTCCCTCGGACCGCAGCTTCCTGACGCCGCGATGAGCGCGGGATCTGAAACCGCGCTGATC.GC.C.GG.GGG.GGCGCGCTGCCGGGGCTGCTTTTGCAGGCCGCGCCGGAAATGCTGGTCTGCGCGCTGGAAGGTCAGCCCGCGCCGGGCGGATCGGTGCT  >  NC_014034/1768802‑1768996

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG