Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 90.3% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G90.3% 39.0 ‑2.4 ‑0.1145coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (61/69):  ref base (3/11):  total (65/80)
Fisher's exact test strand distribution p-value = 9.06e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.34e-01

Only 100 of 160 total aligned reads displayed.

TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.GGCA.GGGG.CGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462
                                                                                                       |                                                                                              
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTc                                                                                                 >  1:2973548/1‑101 (MQ=255)
  tgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTcgc                                                                                               <  2:16920/101‑3 (MQ=255)
    cGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTcgcgc                                                                                             <  1:2441808/101‑5 (MQ=255)
    cGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTcgcgc                                                                                             <  1:122889/101‑5 (MQ=255)
     ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTcgcgcg                                                                                            >  2:1772491/1‑96 (MQ=255)
        aGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTcgcgcgcgg                                                                                         >  1:1934244/1‑93 (MQ=255)
          cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGAGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTcgcgcgcgggc                                                                                       >  2:2117491/1‑91 (MQ=255)
            tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCtt                                                                                   <  1:55968/101‑1 (MQ=255)
            tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCtt                                                                                   >  1:831533/1‑101 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCttt                                                                                  <  1:1631007/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCttt                                                                                  <  2:246608/101‑1 (MQ=255)
              ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTg                                                                                 <  2:601154/101‑1 (MQ=255)
              ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTg                                                                                 <  2:1643402/101‑1 (MQ=255)
              ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTg                                                                                 <  2:850083/101‑1 (MQ=255)
               gCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGc                                                                                <  1:2751627/101‑1 (MQ=255)
                cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGcc                                                                               >  1:198257/1‑101 (MQ=255)
                cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGcc                                                                               <  1:3157477/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                             <  1:3478048/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCtt                                                                             <  2:4235939/101‑1 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTg                                                                            <  2:264408/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:3208191/1‑101 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  1:3304261/1‑101 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:908845/1‑101 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                          >  2:4139833/1‑101 (MQ=255)
                      ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgggg                                                                         >  2:844494/1‑101 (MQ=255)
                      ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTgggg                                                                         >  1:1424905/1‑101 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:3616906/101‑1 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        >  2:1808462/1‑101 (MQ=255)
                       gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGt                                                                        <  1:730655/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  1:2507998/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     >  2:4633083/1‑101 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:1810960/101‑1 (MQ=255)
                           aaCTGGATGCGGCCCGCGCGGCGGAAAGCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:2849801/101‑1 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:4529456/1‑101 (MQ=255)
                              tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:3355500/1‑101 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:4353919/101‑1 (MQ=255)
                                  tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  1:1772600/101‑1 (MQ=255)
                                  tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  1:2050030/101‑1 (MQ=255)
                                   gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  2:4429627/101‑1 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  1:2095027/101‑1 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        >  2:1061098/1‑101 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  1:4504023/101‑1 (MQ=255)
                                        ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       >  1:1222116/1‑101 (MQ=255)
                                          gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGGAcc                                                     >  1:964359/1‑101 (MQ=255)
                                          gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  2:532919/101‑1 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  1:1128262/101‑1 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    >  1:4592743/1‑101 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:1424811/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.TGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:3814928/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  1:518414/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:1157146/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:1548760/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  1:2100329/1‑101 (MQ=255)
                                              ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 >  1:3928756/1‑101 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               <  1:2905878/101‑1 (MQ=255)
                                                 ggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              >  1:1462312/1‑101 (MQ=255)
                                                 ggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              >  2:3213709/1‑101 (MQ=255)
                                                 ggAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              >  2:252409/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGGTTTGCCTTGGGGTGGAGGCGCTTCCGGCCACCGACgcgcc                                             >  1:916047/1‑100 (MQ=255)
                                                   aaaTCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            <  1:2561107/101‑1 (MQ=255)
                                                     aTCGTCGCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          >  2:3656706/1‑101 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATG.TCGGGGAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAtagatcggaag                                        >  1:4207818/1‑91 (MQ=255)
                                                       cgtcgCGGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:2304435/1‑101 (MQ=255)
                                                         gcgcgGTTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  1:3395024/100‑1 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGTTG.GGTAGGGGG.CTGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:2333597/101‑1 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:4746479/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   >  2:1157169/1‑101 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:4633339/101‑1 (MQ=255)
                                                              gatttGGGCGCGGTGGATG.TCGGGCA.GGGGTGGGTGGTCGAG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 <  2:3940156/99‑1 (MQ=255)
                                                              gCTTTGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 <  1:2617238/101‑1 (MQ=255)
                                                                tttGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  1:1138711/1‑101 (MQ=255)
                                                                 ttGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              >  2:1009086/1‑101 (MQ=255)
                                                                 ttGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGACGCGCTCCTTGATTTCGTcgc                              >  1:3879253/1‑101 (MQ=255)
                                                                  tGGGCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCACG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:2276787/1‑101 (MQ=255)
                                                                   gggCGCGGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                            >  1:2817201/1‑101 (MQ=255)
                                                                      cgcgGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCgcgc                         >  2:4181318/1‑101 (MQ=255)
                                                                      cgcgGTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCgcgc                         >  2:2002349/1‑101 (MQ=255)
                                                                         ggTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  2:2907208/1‑101 (MQ=255)
                                                                         ggTCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  2:1899250/1‑101 (MQ=255)
                                                                           tCGATG.TCTGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    <  2:3035908/101‑1 (MQ=255)
                                                                           tCGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    <  2:1899869/101‑1 (MQ=255)
                                                                            cGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  2:1684286/1‑101 (MQ=255)
                                                                            cGATG.TCGGGCAGGGGG.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  2:4620952/1‑101 (MQ=255)
                                                                                  tcggggaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  1:884985/93‑1 (MQ=255)
                                                                                  tcgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              >  1:1317553/9‑101 (MQ=255)
                                                                                  tcgggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  1:4496912/93‑1 (MQ=255)
                                                                                    gggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            <  2:1321919/95‑1 (MQ=255)
                                                                                    gggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  2:2822034/7‑101 (MQ=255)
                                                                                     ggcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCATGGAGTCGAGGCGCTTCCGGGCAACGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg           <  1:4062050/96‑1 (MQ=255)
                                                                                      gcaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:2973360/97‑1 (MQ=255)
                                                                                       gaggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  2:3879011/98‑1 (MQ=255)
                                                                                       caggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  2:2426842/4‑101 (MQ=255)
                                                                                        aggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  1:3513195/3‑101 (MQ=255)
                                                                                        aggggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  1:748985/3‑101 (MQ=255)
                                                                                          gggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:4640789/101‑1 (MQ=255)
                                                                                           ggg.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:600101/101‑1 (MQ=255)
                                                                                            gg.CGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    >  2:4261101/1‑101 (MQ=255)
                                                                                             g.CGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGGCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   >  2:1570725/1‑101 (MQ=255)
                                                                                               cGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  2:2434839/101‑1 (MQ=255)
                                                                                               cGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  2:3592931/101‑1 (MQ=255)
                                                                                                       |                                                                                              
TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATG.TC.GGCA.GGGG.CGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG