Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,823,740 +C 100% intergenic (+25/‑148) RCAP_rcc01687 → / → RCAP_rcc01688 HK97 family phage major capsid protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,823,7401.→C100.0% 52.6 158intergenic (+25/‑148)RCAP_rcc01687/RCAP_rcc01688HK97 family phage major capsid protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (93/47):  ref base (0/18):  total (93/65)
Fisher's exact test strand distribution p-value = 2.32e-08
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.17e-01

Only 100 of 192 total aligned reads displayed.

CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCG  >  NC_014034/1823640‑1823833
                                                                                                       |                                                                                               
ctATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.a.a                                                                                                  >  2:3676700/1‑101 (MQ=255)
ctATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.a.a                                                                                                  <  2:1382972/101‑1 (MQ=255)
ctATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.a.a                                                                                                  >  2:501976/1‑101 (MQ=255)
 tATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.c                                                                                                <  1:1024680/101‑1 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.cc                                                                                               >  2:4291682/1‑101 (MQ=255)
  aTGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.cc                                                                                               >  1:4527340/1‑101 (MQ=255)
   tGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccc                                                                                              <  2:4385305/101‑1 (MQ=255)
    gCGAGCCAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.cccc                                                                                             <  1:1111319/101‑2 (MQ=255)
    gCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.cccc                                                                                             <  2:1608274/101‑2 (MQ=255)
     cGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccg                                                                                            >  2:1118355/1‑99 (MQ=255)
      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGTCGCCTCGGAACGGGCGGTGAAGGGGGCGGGGGT.A.C.ccccgg                                                                                           >  1:453268/1‑98 (MQ=255)
      gAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccgg                                                                                           >  2:4467416/1‑98 (MQ=255)
       aGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccggc                                                                                          >  1:3091183/1‑97 (MQ=255)
       aGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccggc                                                                                          <  2:853608/101‑5 (MQ=255)
        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccggct                                                                                         <  2:4532068/101‑6 (MQ=255)
        gCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.ccccggct                                                                                         >  2:3058398/1‑96 (MQ=255)
         cAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGACGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTg                                                                                        >  1:1891671/1‑101 (MQ=255)
          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgc                                                                                       >  1:872790/1‑101 (MQ=255)
          aaGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgc                                                                                       >  2:3733223/1‑101 (MQ=255)
             cGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgc                                                                                    >  1:1434972/1‑101 (MQ=255)
             cGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgc                                                                                    >  2:1340245/1‑101 (MQ=255)
             cGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAAAGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgc                                                                                    <  1:2723097/101‑1 (MQ=255)
              gggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTgccgcc                                                                                   <  1:2824338/101‑1 (MQ=255)
               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGccgcc.g                                                                                 <  1:492158/101‑1 (MQ=255)
               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGccgcc.g                                                                                 >  2:331582/1‑101 (MQ=255)
               ggTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGccgcc.g                                                                                 <  1:1752614/101‑1 (MQ=255)
                gTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.gg                                                                                >  1:1508051/1‑101 (MQ=255)
                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGtt                                                                              >  2:3307977/1‑101 (MQ=255)
                  gggCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGtt                                                                              >  2:3864315/1‑101 (MQ=255)
                    gcggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCg                                                                            <  2:1798945/101‑1 (MQ=255)
                    gcggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCg                                                                            >  1:717161/1‑101 (MQ=255)
                     cggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGc                                                                           >  1:2434924/1‑101 (MQ=255)
                     cggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGc                                                                           <  2:3090983/101‑1 (MQ=255)
                      ggcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGcc                                                                          >  1:1244835/1‑101 (MQ=255)
                       gcgATGTCAGCTATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCg                                                                         <  2:1103680/101‑1 (MQ=255)
                       gcgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCg                                                                         >  2:2586587/1‑101 (MQ=255)
                        cgATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCgg                                                                        <  2:3996869/101‑1 (MQ=255)
                            gTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTcgg                                                                    >  1:3634979/1‑101 (MQ=255)
                              cAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTcgggc                                                                  <  1:2754762/101‑1 (MQ=255)
                               aGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTcgggcg                                                                 >  1:1693665/1‑101 (MQ=255)
                                gCGATTCCGCCGCGTTCAAGCTGTTGAAGTTTGCCTCTTCGTAACGGGCGGTGAGGGGGCCGGGGGC.ATC.CCC.GGCTGCCGCC.GGTTCGCCGGTcgggcgg                                                                <  2:3599673/101‑1 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCgggcgggc                                                              >  2:4086238/1‑101 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCgggcgggc                                                              >  2:341659/1‑101 (MQ=255)
                                  gATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCgggcgggc                                                              <  2:3467041/101‑1 (MQ=255)
                                  gATTCCCCCGCGATCAATTTTTTGAATTTTGCCGGCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGTTGCCGCC.GGTTCGCCGGTCgggcgggc                                                              <  1:371846/101‑1 (MQ=255)
                                    tttCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgc                                                            <  2:2616628/101‑1 (MQ=255)
                                     ttCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgcg                                                           >  1:456401/1‑101 (MQ=255)
                                     ttCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgcg                                                           >  1:1414413/1‑101 (MQ=255)
                                     ttCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgcg                                                           >  1:2153050/1‑101 (MQ=255)
                                     ttCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgcg                                                           >  1:1448289/1‑101 (MQ=255)
                                       cgccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgcggg                                                         >  1:2840825/1‑101 (MQ=255)
                                        gccgcGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGgcgggcgcgggc                                                        >  1:3168389/1‑101 (MQ=255)
                                          cgcgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCtt                                                      <  2:1507950/101‑1 (MQ=255)
                                            cgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                    >  2:1624072/1‑101 (MQ=255)
                                            cgATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGGGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTcc                                                    <  1:563113/101‑1 (MQ=255)
                                              aTCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCgg                                                  <  2:4472226/101‑1 (MQ=255)
                                               tCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGc                                                 <  1:3485537/101‑1 (MQ=255)
                                                cAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGcc                                                >  2:2288778/1‑101 (MQ=255)
                                                cAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGcc                                                <  2:4318453/101‑1 (MQ=255)
                                                 aaGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGccc                                               >  1:2174951/1‑100 (MQ=255)
                                                  aGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGc                                              <  2:2786724/101‑1 (MQ=255)
                                                     tGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGc                                           <  1:3339161/101‑1 (MQ=255)
                                                     tGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGA.A.A.CCC.CGCTTCCCCCGGGTCCGCGGGCCGGGCGGGGGGGGGCTTCC.GCCGCCGcc                                          >  1:1848103/1‑101 (MQ=255)
                                                      gTTGAAGTTTGCCGCCTCGTAACGGCCGGAGAAGGGGCCGGGGGCAA.A.CCC.GGCTGCCGCC.GGCGCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTgac                                          >  2:246082/1‑99 (MQ=255)
                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                         <  2:1190428/101‑1 (MQ=255)
                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTGCGGCCGCTGCCt                                         <  1:2578098/101‑1 (MQ=255)
                                                       ttGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                         <  1:3862579/101‑1 (MQ=255)
                                                       tgcaaGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCt                                         <  2:3243998/98‑1 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                        <  2:2237383/101‑1 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                        <  2:4290835/101‑1 (MQ=255)
                                                        tGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTa                                        <  2:2712331/101‑1 (MQ=255)
                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                    <  1:1570262/101‑1 (MQ=255)
                                                            gTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctg                                    >  2:4510134/1‑101 (MQ=255)
                                                              ttGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAgctgct                                  >  1:659983/1‑101 (MQ=255)
                                                                gccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctcc                                >  2:1990965/1‑101 (MQ=255)
                                                                 ccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccc                               <  1:1283597/101‑1 (MQ=255)
                                                                 ccgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccc                               >  2:1051345/1‑101 (MQ=255)
                                                                  cgccTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGctccct                              <  2:3168189/101‑1 (MQ=255)
                                                                     cgcGTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                           <  2:1870648/99‑1 (MQ=255)
                                                                     cTCGTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                           <  1:3423848/101‑1 (MQ=255)
                                                                     cTCCTAGCGGGCGGTGATGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccg                           <  2:4268309/101‑1 (MQ=255)
                                                                        gTAACGGGCGGTGAAGGGGCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                        <  2:2152926/101‑1 (MQ=255)
                                                                        gTAACGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCGGGTCGGGCGGTCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtcc                        >  2:1146236/1‑101 (MQ=255)
                                                                         gaaCGGGCGGTGAAGGGGCGGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCtccgtccg                       <  2:782879/100‑1 (MQ=255)
                                                                          aaCGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                      >  2:471409/1‑101 (MQ=255)
                                                                          aaCGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGa                      >  2:2382432/1‑101 (MQ=255)
                                                                           aCGGGCGGGGAAGGGGCGGGGGGC.A.ACCCC.GGCGGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAg                     <  1:4393271/101‑1 (MQ=255)
                                                                            cGGGCGGTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGt                    >  1:3916756/1‑101 (MQ=255)
                                                                                 ggTGAAGGGGCGGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCgg               <  1:2951939/101‑1 (MQ=255)
                                                                                  gTGAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGt              <  2:2174827/101‑1 (MQ=255)
                                                                                    gAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCggggg            >  2:3277113/1‑98 (MQ=255)
                                                                                    gAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGc            >  1:1545867/1‑101 (MQ=255)
                                                                                    gAAGGGGCCGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGc            >  1:1473361/1‑101 (MQ=255)
                                                                                       ggggCCGGGGGC.ACC.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCggg         <  1:2050471/101‑1 (MQ=255)
                                                                                         ggCCGGGGGC.A.C.CCCGGGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGt       <  1:4691833/101‑1 (MQ=255)
                                                                                           gggggggC.A.CCCCC.GGCTCCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCt     <  1:1146322/99‑1 (MQ=255)
                                                                                           ccGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGGCt     >  2:93895/1‑101 (MQ=255)
                                                                                            cGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTg    <  2:3634750/101‑1 (MQ=255)
                                                                                            cGGGGGC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGGCTg    >  1:2284946/1‑101 (MQ=255)
                                                                                              ggggC.A.ACCCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTgcg  >  2:2891249/1‑101 (MQ=255)
                                                                                                       |                                                                                               
CTATGCGAGCAAGCGGGTGGGCGGCGATGTCAGCGATTTCGCCGCGATCAAGCTGTTGAAGTTTGCCGCCTCGTAACGGGCGGTGAAGGGGCCGGGGGC.A.A.CCC.GGCTGCCGCC.GGTTCGCCGGTCGGGCGGGCGCGGGCTTCCGGCCGCTGCCTAGCTGCTCCCTCCGTCCGAGTGGCGGTGCGGGGTCTGCG  >  NC_014034/1823640‑1823833

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG