BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,843,426	+G	100%	intergenic (+88/+60)	ppiB → / ← tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,843,426	1	.→G	100.0%	551.4	173	intergenic (+88/+60)	ppiB/tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): new base (98/74): ref base (1/0): total (99/74)

Only 100 of 187 total aligned reads displayed.


CGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.G.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTGC  >  NC_014034/1843328‑1843520


cGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCt                                                                                                     <  2:1953948/101‑1 (MQ=25)


       gggACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcg                                                                                             <  1:1654839/99‑4 (MQ=21)


              gggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTc                                                                                      <  2:2253713/101‑1 (MQ=21)


              gggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTc                                                                                      <  2:3533597/101‑1 (MQ=21)


               ggcggaggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCa                                                                                     <  2:3147639/94‑1 (MQ=18)


               ggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCa                                                                                     <  1:3842010/101‑1 (MQ=21)


                gcgcAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCAt                                                                                    <  2:2702671/101‑1 (MQ=21)


                     ggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGc                                                                               <  1:173567/100‑1 (MQ=21)


                      gggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGcc                                                                              <  1:2986119/101‑1 (MQ=21)


                         ggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCcaca                                                                           <  1:646910/101‑1 (MQ=25)


                         ggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCcaca                                                                           <  1:3453191/101‑1 (MQ=25)


                          gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACaa                                                                          >  1:2250568/1‑101 (MQ=25)


                          gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACaa                                                                          >  2:1085910/1‑101 (MQ=25)


                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACaaa                                                                         >  2:3744030/1‑101 (MQ=25)


                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACaaa                                                                         >  1:3711625/1‑101 (MQ=25)


                             tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAat                                                                        >  1:4726029/5‑101 (MQ=25)


                             tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAat                                                                        <  2:1874133/97‑1 (MQ=25)


                              ctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAata                                                                       >  1:4551615/4‑101 (MQ=25)


                               tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAatat                                                                      >  1:4492059/3‑101 (MQ=25)


                               tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAatat                                                                      <  2:4463913/99‑1 (MQ=25)


                               tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAatat                                                                      >  2:1067481/3‑101 (MQ=25)


                                gccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATc                                                                     <  2:2436387/100‑1 (MQ=37)


                                 ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATcc                                                                    <  1:747177/101‑1 (MQ=37)


                                 ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATcc                                                                    <  1:1471962/101‑1 (MQ=37)


                                 ccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATcc                                                                    <  1:3181150/101‑1 (MQ=37)


                                  cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATccc                                                                   <  2:2610018/101‑1 (MQ=37)


                                  cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATccc                                                                   <  2:281233/101‑1 (MQ=37)


                                     ccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCggg                                                                <  1:2355782/101‑1 (MQ=37)


                                          gctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g                                                         >  1:3774305/3‑101 (MQ=38)


                                           ctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.g                                                       >  2:3230767/2‑101 (MQ=38)


                                              cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.gggc                                                    <  2:2996253/101‑3 (MQ=38)


                                               gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.gggca                                                   <  2:1166402/101‑4 (MQ=38)


                                                cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.gggcag                                                  >  1:2314356/1‑97 (MQ=37)


                                                cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.gggcag                                                  >  2:4577133/1‑97 (MQ=37)


                                                cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.gggcag                                                  <  2:1659036/101‑5 (MQ=37)


                                                cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.G.GGGaga                                                  >  2:3346968/1‑101 (MQ=38)


                                                 aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.g.gggcaga                                                 >  2:251849/1‑96 (MQ=37)


                                                  ttCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCagag                                                 >  1:1575221/1‑101 (MQ=37)


                                                  ttCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCagag                                                 >  1:4304198/1‑101 (MQ=37)


                                                   tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGc                                                >  2:3063181/1‑101 (MQ=37)


                                                    cACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGcc                                               >  2:3792446/1‑101 (MQ=25)


                                                      ccccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGcccc                                             <  1:2802557/101‑1 (MQ=25)


                                                      ccccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGcccc                                             <  1:2278386/101‑1 (MQ=25)


                                                          ccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGcc                                         <  1:944427/101‑1 (MQ=25)


                                                          ccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGcc                                         >  1:3003270/1‑101 (MQ=25)


                                                           cGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCt                                        <  2:3858673/101‑1 (MQ=25)


                                                            gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCtt                                       <  2:162570/101‑1 (MQ=25)


                                                            gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCtt                                       <  1:4391504/101‑1 (MQ=25)


                                                            gggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCtt                                       <  2:4028375/101‑1 (MQ=25)


                                                             ggATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTg                                      >  2:1705606/1‑101 (MQ=25)


                                                              gATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.GCGGCAGAGCCCCGGCCTTGc                                     >  2:4752458/1‑101 (MQ=25)


                                                               atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATTTCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCt                                    >  1:4067363/1‑101 (MQ=25)


                                                               atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCt                                    >  1:3678377/1‑101 (MQ=25)


                                                               atatTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCt                                    >  2:4172581/1‑101 (MQ=25)


                                                                 atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCa                                  >  2:820350/1‑101 (MQ=25)


                                                                 atTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCa                                  <  1:1348809/101‑1 (MQ=25)


                                                                 atTTTCGCCAAGGTGAAATCGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCa                                  >  2:133386/1‑101 (MQ=25)


                                                                  ttttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAg                                 <  1:1067560/101‑1 (MQ=25)


                                                                   tttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGc                                >  1:1140124/1‑101 (MQ=25)


                                                                    ttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGTCCC.G.GGCAGCGCCCGGGCCTTGCTCGGcc                               >  2:3639364/1‑101 (MQ=25)


                                                                    ttCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGcc                               >  2:2025402/1‑101 (MQ=25)


                                                                      cGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CTCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCa                             <  1:4688599/101‑1 (MQ=25)


                                                                      cGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCa                             >  2:471426/1‑101 (MQ=25)


                                                                       gCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCag                            <  1:1399187/101‑1 (MQ=25)


                                                                        ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCaga                           >  1:1314753/1‑101 (MQ=25)


                                                                        ccAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCaga                           >  2:2534817/1‑101 (MQ=25)


                                                                         cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCagag                          >  2:75915/1‑101 (MQ=25)


                                                                         cAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCagag                          >  2:2682759/1‑101 (MQ=25)


                                                                           aGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                        >  2:3202563/1‑101 (MQ=25)


                                                                           aGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                        <  1:1085996/101‑1 (MQ=25)


                                                                           aGGTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                        <  1:3354987/101‑1 (MQ=25)


                                                                            ggTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGgca                       >  2:3765107/1‑101 (MQ=25)


                                                                            ggTGAAATGGAAAAGGGCGCTCCACTGGGGG.CACCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGaca                       <  1:3120411/101‑1 (MQ=21)


                                                                             gTGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGacac                      <  2:410097/101‑1 (MQ=25)


                                                                              tGAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc                     >  1:3863927/1‑101 (MQ=25)


                                                                               gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                    >  1:3786590/1‑101 (MQ=25)


                                                                               gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                    <  1:1517069/101‑1 (MQ=25)


                                                                               gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                    >  1:2037332/1‑101 (MQ=25)


                                                                               gAAATGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa                    <  2:3774071/101‑1 (MQ=25)


                                                                                aaaTGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg                   >  2:445389/1‑101 (MQ=25)


                                                                                   tGGAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc                >  1:61803/1‑101 (MQ=25)


                                                                                    ggAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa               >  1:854671/1‑101 (MQ=25)


                                                                                    ggAAAAGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa               >  1:389895/1‑101 (MQ=25)


                                                                                    ggAAAAGGGCGCTCCGCTGGGGG.CGACCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa               >  2:4695325/1‑101 (MQ=25)


                                                                                      aaaaGGGCGCCCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg             <  2:4622440/101‑1 (MQ=25)


                                                                                       aaaGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg            >  1:416228/1‑101 (MQ=25)


                                                                                         aGGGCGCTCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcg          >  2:3211199/1‑101 (MQ=25)


                                                                                          gggcgctCCGGT.GGGGCCGCCTTTTCATCTTCCCACACATCTCCCGGGGC.CCGG.GGCGGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcgc         <  1:3304891/95‑1 (MQ=255)


                                                                                          gggcgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcgc         >  1:3743588/7‑101 (MQ=25)


                                                                                          gggcgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAACATCCCCGGGT.CCGG.GGGAGAGGCCTGGCCTTGCTCAGCCCAGAGACACCATCGCATGgctc         >  2:1509255/7‑99 (MQ=19)


                                                                                             cgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttc      >  1:3158365/4‑101 (MQ=25)


                                                                                             cgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttc      >  1:3969554/4‑101 (MQ=25)


                                                                                             cgctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttc      >  2:1411191/4‑101 (MQ=25)


                                                                                              gctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttct     <  1:2105745/99‑1 (MQ=37)


                                                                                              gctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttct     >  1:2793954/3‑101 (MQ=37)


                                                                                               ctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttctt    >  1:3232737/2‑101 (MQ=37)


                                                                                               ctCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGCT.CC.GAGGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttctt    <  2:551634/100‑1 (MQ=37)


                                                                                                tCCGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTg   <  1:251874/101‑1 (MQ=37)


                                                                                                 ccGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CCGG.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTGc  >  2:1237455/1‑101 (MQ=37)


                                                                                                 ccGCTGGGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGA.CC.GTGGCAGAGCCCCGGCCTTGCTCAGTCCAGAGATACCATCGCCTGCCGCTTCTTGc  >  2:2894685/1‑101 (MQ=21)


CGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGG.CGCCCTTTCATCTTGCCACAAATATCCCGGGGT.CC.G.GGCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTGC  >  NC_014034/1843328‑1843520

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG