Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,867,831 +C 5.3% intergenic (‑233/+82) RCAP_rcc01729 ← / ← RCAP_rcc01730 pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,867,8311.→C 5.3% 5.9 ‑2.1 ‑0.076intergenic (‑233/+82)RCAP_rcc01729/RCAP_rcc01730pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (33/39):  total (33/43)
Fisher's exact test strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.49e-01

Only 100 of 170 total aligned reads displayed.

AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.G.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867731‑1867927
                                                                                                       |                                                                                                      
aGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.Ag                                                                                                         <  1:4193184/101‑1 (MQ=255)
  gAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGG.A.Aa.c.g                                                                                                     >  2:3068121/1‑101 (MQ=37)
   aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCCGCCCCGCGGGGCCGA.A.Ac.c.g.c                                                                                                   >  2:1328980/1‑101 (MQ=37)
      aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGGGCGA.A.Ag.c.ggccg                                                                                                 >  1:2782469/1‑97 (MQ=255)
       gCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.c.g.cccgc                                                                                               <  2:1004535/101‑4 (MQ=255)
       gCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.c.g.cccgc                                                                                               <  1:1067540/101‑4 (MQ=255)
        cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.c.g.cccgcc                                                                                              <  2:3914035/101‑5 (MQ=255)
        cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCCGCGGCGCGA.A.AG.C.GAccgcc                                                                                               >  2:2393713/1‑101 (MQ=37)
          cTGCTTTCCCCCGCCGGTGTCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCAt                                                                                             <  2:54682/101‑1 (MQ=25)
          cTGCTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCAt                                                                                             <  2:1902339/101‑1 (MQ=255)
           tccTTTCCCCCGCCCGTGTCGCGACGGCTTTGCAAAGCCCGCACCCCCAAGCGGGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCAA.A.AG.C.GCCCGCCATg                                                                                            <  2:1736076/99‑1 (MQ=21)
           tGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGC.A.AG.c.g.cccgccacg                                                                                           >  1:4238673/1‑94 (MQ=37)
             cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AA.CGG.CCGCCACggg                                                                                          >  1:3719027/1‑101 (MQ=37)
                tcccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcgg                                                                                       <  2:4054453/99‑1 (MQ=255)
                 tCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCCGCGGCGCGC.A.AG.C.GCCCCCCACgggcggg                                                                                      >  2:3200353/1‑101 (MQ=25)
                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcggggc                                                                                    <  2:2113388/101‑1 (MQ=255)
                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcggggc                                                                                    <  2:2825120/101‑1 (MQ=255)
                   cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcggggc                                                                                    <  2:466865/101‑1 (MQ=255)
                     cgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCCGCCCCGGCGGCGCGG.A.AG.A.GCCCCCCCTGggcggggg.gg                                                                                 >  2:3680239/1‑101 (MQ=18)
                       cccgcctCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCACCCCCGGCGGCGCAA.A.AGCC.C.CCGCCATGGGCGggtc.gggc                                                                               <  2:1984277/95‑1 (MQ=18)
                       cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.A.AG.CGG.CCGCCCCGGgcggggg.gggg                                                                               >  2:3657486/1‑100 (MQ=21)
                         cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCAAGGGCGGGGC.GGggg.c                                                                            >  2:2908094/1‑101 (MQ=21)
                         cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGC.A.AA.C.GCCCGCCCTGGGGGGGGG.GGggg.c                                                                            >  1:4021446/1‑101 (MQ=255)
                          gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.Ct                                                                           <  2:2785740/101‑1 (MQ=255)
                          gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCCTGGGCGGGGG.GGggg.cc                                                                           >  2:1723372/1‑100 (MQ=21)
                          gtgtCGCGACAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.Ct                                                                           <  1:3680468/101‑1 (MQ=255)
                           tgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGGGACA.AC.G.G.CCCCCAATGGCGGGGG.GGggg.cgg                                                                          >  2:1465192/1‑99 (MQ=18)
                            gtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCTGGGC.GGGCG.CTgg                                                                         <  2:2782304/101‑1 (MQ=255)
                             tCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGc                                                                        <  1:3782903/101‑1 (MQ=255)
                              cgcgACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGcc                                                                       <  1:384559/101‑1 (MQ=255)
                               gcgACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCg                                                                      <  1:3365296/101‑1 (MQ=255)
                               gcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCg                                                                      <  1:2172896/101‑1 (MQ=255)
                                cgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCCCGGGCGGGGC.GGGGG.CCGGGCgg                                                                     >  1:3288061/1‑100 (MQ=18)
                                cgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACACCCCACCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGgcg.cgtggcga                                                                     >  2:2343341/1‑94 (MQ=17)
                                 gACAGCGTTCCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.g                                                                   <  2:3945570/101‑1 (MQ=17)
                                   aaccgtggaaaaGGCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.ggg                                                                 <  2:4354083/92‑1 (MQ=14)
                                      cGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAACCCCGGCGGCGCGA.A.AG.C.GCCCGCCACGGGCGGGGC.GGGCG.GCGGcccgggggcc                                                               >  1:2328567/1‑93 (MQ=17)
                                      cGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGG.A.AG.C.GCCCGCCCTGGGCGGGGC.GGGGG.CCGGGCCA.GGG.GCt                                                             >  2:257199/1‑101 (MQ=17)
                                         tGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGG.GGGGG.CGGGCCGA.GGG.GCTTTg                                                          >  1:1929718/1‑101 (MQ=18)
                                          gCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCCTGGGCGGGGG.GGGGG.CCGGGCGC.GGG.GCCTTgg                                                         >  2:3412238/1‑101 (MQ=17)
                                          gCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTgg                                                         <  1:4323134/101‑1 (MQ=17)
                                           aaaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTggg                                                        <  2:3006605/100‑1 (MQ=255)
                                            aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGGG.CCGGCCGG.GGG.GCTTTgggg                                                       >  1:1846674/1‑101 (MQ=17)
                                            aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGGG.CGGGCCGA.GGG.GCTTTtggg                                                       >  2:3234801/1‑101 (MQ=17)
                                            aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCCCCCTGGGCGGGGC.GGGCG.CTCGCCCA.GGG.CCCCTgggg                                                       >  1:1790637/1‑101 (MQ=17)
                                             aaGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGc                                                      <  2:646298/101‑1 (MQ=255)
                                             aaGACCGCATCCCCAAGCGCGGGAGAGGCCAGCGCCCCGCCCCGGCGGCGCGA.A.AA.C.GCCCGCCATGGGCGGGGG.GGGGGCCGGGCGGG.GGG.CCTTTgggg                                                       >  1:2697032/1‑101 (MQ=11)
                                              agcccGCACCCCCAGGCGCGCGAGAGGCCAGCCCCCACCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGcc                                                     <  1:2217149/98‑1 (MQ=17)
                                              agcccGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCGGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGcc                                                     <  2:975486/98‑1 (MQ=17)
                                              aGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGG.GGGCG.CCGGCCGC.GGG.CCCTTgggggc                                                     >  2:2199164/1‑99 (MQ=14)
                                              aGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CCGGCCCA.GGG.GCCTTgggggc                                                     >  1:226936/1‑99 (MQ=17)
                                               gcccGCACCCCCAAGCGGGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGccc                                                    <  1:1410313/99‑1 (MQ=17)
                                                cccccacccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGcccc                                                   <  1:3121839/94‑1 (MQ=17)
                                                 ccGCACCCCGAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGccccc                                                  <  2:1653819/101‑1 (MQ=17)
                                                   gCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CCGGCCCA.GGG.GCTTTGGGGgcccccg                                                >  2:1534129/1‑99 (MQ=17)
                                                    cctCCCCAAGCGCGCGGGAGGCCTGCGTCCCGCCCCGGCGGCGCGA.A.TG.C.GCCCGCCATGGGCGGGGC.GGGCG.CCGGGCGA.AGG.CCGCCGGGGCCCCcggg                                               >  2:564940/3‑100 (MQ=17)
                                                    cacccccAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCcggc                                               <  1:3541273/98‑1 (MQ=17)
                                                    cATCCCCAGGCGCGCGAGAGGCCAGCGCCCTGCCGCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCcggc                                               <  1:1563152/101‑1 (MQ=17)
                                                     aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGGGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGGG.CTGGCCGA.GGGCCCTTTGGGGCCCCcgcg                                               >  2:425338/1‑99 (MQ=17)
                                                      cccccAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCcggcgg                                             <  2:3761235/100‑1 (MQ=255)
                                                       ccccAGGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGt                                            <  1:3822991/101‑1 (MQ=17)
                                                       ccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGGCGG.GGG.CCCTTGGGGGCCCcggcggg                                            >  1:4404547/1‑100 (MQ=17)
                                                        cccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTc                                           <  1:3068309/101‑1 (MQ=255)
                                                         ccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.GCCTTGGGGGCCCccgcgggcg                                          >  2:551995/1‑98 (MQ=14)
                                                            ggcgcgcGAGAGGCCGGCGCCCGGCCCCGGCGGCGCGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAggg                                       <  1:209189/100‑1 (MQ=14)
                                                            aGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CCGGCCGA.GGG.GCCTTTGGGCCCCCGGCGGGCAggg                                       >  2:2102885/1‑101 (MQ=14)
                                                             gcgcgcGAGGGGCCGGCCCCCGGCCCCGGCGGCGCGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgggg                                      <  1:1688815/101‑1 (MQ=14)
                                                             ccgcgcgAGAGCCCACCACCGAGCCCCGGCGCGGCGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgggg                                      <  2:2971823/100‑1 (MQ=14)
                                                              cgcgcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.GCCTTGGGGGCCCCCGCGGGCCggggg                                     >  2:2374513/1‑101 (MQ=14)
                                                              cacgacagagGCCTGCGCCCCGCCCCGGCGGCGCGA.A.TG.CGG.CCGCCCAAGGCGGGGCGGGGCG.CAGGCCCG.GGG.CCTTTGGGCCCCCGGGGGGGCCgggc                                      >  2:786682/7‑100 (MQ=14)
                                                               gggcgGGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                    <  2:1089698/99‑1 (MQ=17)
                                                               gcggggggggCCCGCGCCCCGCCCCGGGGGCGGGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                    <  1:1329072/93‑1 (MQ=14)
                                                               gcgcgAGGGGCCAGCGCCCGGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                    <  2:2479381/101‑1 (MQ=17)
                                                                  ggagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                 <  1:2557067/100‑1 (MQ=255)
                                                                  cgAGAGGCCAGCCCCGAGCCCGGGCGGCCCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                 <  2:3557051/101‑1 (MQ=17)
                                                                   gggagGCCGGCCCCCCGCCCCGGCGGGGCAA.A.GC.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  1:4051705/99‑1 (MQ=14)
                                                                   gaggGGCCGGCCCCCGGCCCCGGGGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGT.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  1:4635095/101‑1 (MQ=17)
                                                                    agagGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CCGGCCGAGGGG.CCTTTGGGG.CCCCGGCGGGCAcggggggccgt                               >  2:3424608/1‑95 (MQ=11)
                                                                     ggggCCACCCCCCAGCCCCGGCGGCGCGA.A.CGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  1:3200554/99‑1 (MQ=17)
                                                                     gagGCCGGCCCCCACCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  1:1078351/101‑1 (MQ=17)
                                                                      gggCCGGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                             <  1:4275621/100‑1 (MQ=17)
                                                                      agGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGGCAGGGGGGTCTGTTg                             >  1:3326864/1‑101 (MQ=17)
                                                                       ggCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGGCGGGGGGGTCTGTTgg                            >  1:893172/1‑100 (MQ=17)
                                                                       gcccAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                            <  2:1146790/99‑1 (MQ=255)
                                                                          cAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                         >  1:3906125/1‑101 (MQ=255)
                                                                           ggcgcCAAGCCCGGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  1:564773/100‑1 (MQ=18)
                                                                           ggcccccAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  2:3519897/97‑1 (MQ=18)
                                                                             cgcCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                      <  2:3191857/101‑1 (MQ=255)
                                                                               cccCGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                    >  1:3141596/1‑101 (MQ=255)
                                                                                 cAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                  >  1:1922812/1‑101 (MQ=255)
                                                                                  aGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTGCGCATGGCcgcg                 >  2:3647052/1‑101 (MQ=255)
                                                                                   gCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGt                >  1:2933209/1‑101 (MQ=255)
                                                                                    ccgaggcggcGGGA.G.AA.C.GCCCGCCATGGGCGGCGC.GGGCG.CTGGCCGA.GGT.CCTGTGGGGGCCCCGGCGGTCAGGGGCATCTGTTGCGCATGGACGCGTc               <  2:2341654/97‑1 (MQ=18)
                                                                                    cccgGGCGGCGCGA.AGCC.C.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc               <  2:906987/101‑1 (MQ=18)
                                                                                    ccccGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc               >  2:1182250/1‑101 (MQ=255)
                                                                                       cggcggCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGG.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTCGAGCATGGCCGTGGCAgc            >  1:731389/1‑101 (MQ=17)
                                                                                           ggcgCGA.A.GGCC.C.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccg        <  1:189903/101‑1 (MQ=21)
                                                                                                gA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTTGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc   <  1:1534230/101‑1 (MQ=255)
                                                                                                 a.a.aG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa  >  1:84756/1‑101 (MQ=255)
                                                                                                 a.a.aG.C.GCCCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa  >  1:1192207/1‑101 (MQ=255)
                                                                                                       |                                                                                                      
AGGAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.G.CCGCCATGGGCGGGGC.GGGCG.CTGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867731‑1867927

Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG