BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,867,833	+C	100%	intergenic (‑235/+80)	RCAP_rcc01729 ← / ← RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,867,833	1	.→C	100.0%	41.9	99	intergenic (‑235/+80)	RCAP_rcc01729/RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand): new base (33/52): ref base (0/14): total (33/66)
Fisher's exact test strand distribution p-value = 4.05e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.25e-01

Only 100 of 169 total aligned reads displayed.


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.G.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867733‑1867927


gAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGG.A.Aa.c.g                                                                                                      >  2:3068121/1‑101 (MQ=37)


 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCCGCCCCGCGGGGCCGA.A.Ac.c.g.c                                                                                                    >  2:1328980/1‑101 (MQ=37)


 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGG.A.Aa.c.g.c                                                                                                    >  1:948019/1‑101 (MQ=38)


     gCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.c.g.cccgc                                                                                                <  1:1067540/101‑4 (MQ=255)


      cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.c.g.cccgcc                                                                                               <  2:2544514/101‑5 (MQ=255)


      cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCAGCCCCCGCGGCGCGA.A.AG.C.GAccgcc                                                                                                >  2:2393713/1‑101 (MQ=37)


        cTGCTTTCCCCCGCCGGTGTCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCAt                                                                                              <  2:54682/101‑1 (MQ=25)


        cTGCTTTCCCCCGCCGGTGTCGCGACAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCAt                                                                                              <  2:1211188/101‑1 (MQ=25)


         tGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGC.A.AG.c.g.cccgccacg                                                                                            >  1:4238673/1‑94 (MQ=37)


           cTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AA.CGG.CCGCCACggg                                                                                           >  1:3719027/1‑101 (MQ=37)


              tcccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcgg                                                                                        <  2:4054453/99‑1 (MQ=255)


                 cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcggggc                                                                                     <  2:466865/101‑1 (MQ=255)


                 cccgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATgggcggggc                                                                                     <  2:2825120/101‑1 (MQ=255)


                   cgccggTGTCGCGACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGggcggggc.gg                                                                                  <  1:3802497/101‑1 (MQ=21)


                   cgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAAAGCCCCGCCCCGGCGGCGCGG.A.AG.A.GCCCCCCCTGggcggggg.gg                                                                                  >  2:3680239/1‑101 (MQ=18)


                    gcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGgcggggc.ggg                                                                                 <  1:1866946/101‑1 (MQ=255)


                     cccgcctCGCGACAGCGTTGCAAAGCCCGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCACCCCCGGCGGCGCAA.A.AGCC.C.CCGCCATGGGCGggtc.gggc                                                                                <  2:1984277/95‑1 (MQ=18)


                     cccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.A.AG.CGG.CCGCCCCGGgcggggg.gggg                                                                                >  2:3657486/1‑100 (MQ=21)


                      ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGggc.gggcg                                                                               <  2:2803646/101‑1 (MQ=255)


                       cgTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCAAGGGCGGGGC.GGggg.c                                                                             >  2:2908094/1‑101 (MQ=21)


                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCCTGGGCGGGGG.GGggg.cc                                                                            >  2:1723372/1‑100 (MQ=21)


                         tgtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGGGACA.AC.G.G.CCCCCAATGGCGGGGG.GGggg.cgg                                                                           >  2:1465192/1‑99 (MQ=18)


                         gttCGCGACGGCGTTGCAAAGCCCGCATCCCCAAGCGGGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCAA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.Tg                                                                          <  1:4232552/99‑1 (MQ=18)


                          gtCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCTGGGC.GGGCG.C.Tgg                                                                         <  2:2782304/101‑1 (MQ=255)


                           tCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGc                                                                        <  1:3782903/101‑1 (MQ=255)


                            cgcgACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGcc                                                                       <  1:384559/101‑1 (MQ=255)


                            cgcgACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGcc                                                                       <  2:3934949/101‑1 (MQ=255)


                             gcgACAGCGTTGCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCg                                                                      <  1:3365296/101‑1 (MQ=255)


                             gcgACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCg                                                                      <  1:2172896/101‑1 (MQ=255)


                               gACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCCTGGGGGGGGG.GGGGG.C.Tgggccgg                                                                    >  2:1931739/1‑96 (MQ=18)


                               gACAGCGTTCCAAAGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.g                                                                   <  2:3945570/101‑1 (MQ=17)


                                 aaccgtggaaaaGGCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.ggg                                                                 <  2:4354083/92‑1 (MQ=14)


                                    cGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAACCCCGGCGGCGCGA.A.AG.C.GCCCGCCACGGGCGGGGC.GGGCG.G.CGGcccgggggcc                                                               >  1:2328567/1‑93 (MQ=17)


                                    cGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGG.A.AG.C.GCCCGCCCTGGGCGGGGC.GGGGG.C.CGGGCCA.GGG.GCt                                                             >  2:257199/1‑101 (MQ=17)


                                       tGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCCTGGGCGGGGG.GGGGG.CGGGGCCGG.GGG.CCttg                                                           >  2:3215426/1‑100 (MQ=17)


                                       tGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGG.GGGGG.C.GGGCCGA.GGG.GCTTTg                                                          >  1:1929718/1‑101 (MQ=18)


                                        gCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCCTGGGCGGGGG.GGGGG.C.CGGGCGC.GGG.GCCTTgg                                                         >  2:3412238/1‑101 (MQ=17)


                                          aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGGG.C.CGGCCGG.GGG.GCTTTgggg                                                       >  1:1846674/1‑101 (MQ=17)


                                          aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGGG.C.GGGCCGA.GGG.GCTTTtggg                                                       >  2:3234801/1‑101 (MQ=17)


                                          aaaGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGA.A.AG.C.GCCCCCCCTGGGCGGGGC.GGGCG.C.TCGCCCA.GGG.CCCCTgggg                                                       >  1:1790637/1‑101 (MQ=17)


                                           aaGCCCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGc                                                      <  2:646298/101‑1 (MQ=255)


                                           aaGACCGCATCCCCAAGCGCGGGAGAGGCCAGCGCCCCGCCCCGGCGGCGCGA.A.AA.C.GCCCGCCATGGGCGGGGG.GGGGGCC.GGGCGGG.GGG.CCTTTgggg                                                       >  1:2697032/1‑101 (MQ=11)


                                            agcccGCACCCCCAGGCGCGCGAGAGGCCAGCCCCCACCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGcc                                                     <  1:2217149/98‑1 (MQ=17)


                                            agcccGCACCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCGGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGcc                                                     <  2:975486/98‑1 (MQ=17)


                                            aGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCCGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGG.GGGCG.C.CGGCCGC.GGG.CCCTTgggggc                                                     >  2:2199164/1‑99 (MQ=14)


                                            aGACCGCATCCCCAAGCGCGCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.CGGCCCA.GGG.GCCTTgggggc                                                     >  1:226936/1‑99 (MQ=17)


                                             gcccGCACCCCCAGGCGCGCGAGGGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGccc                                                    <  1:1931856/99‑1 (MQ=17)


                                             gcccGCACCCCCAGGCGCGCGAGAGGCCGGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGccc                                                    <  1:436099/99‑1 (MQ=17)


                                             gcccGCACCCCCAAGCGGGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGccc                                                    <  1:1410313/99‑1 (MQ=17)


                                               ccGCACCCCGAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGccccc                                                  <  2:1653819/101‑1 (MQ=17)


                                                 gCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGG.GGG.GCCTTGGGGCCCCcgg                                                >  2:302313/1‑101 (MQ=17)


                                                  cctCCCCAAGCGCGCGGGAGGCCTGCGTCCCGCCCCGGCGGCGCGA.A.TG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.CGGGCGA.AGG.CCGCCGGGGCCCCcggg                                               >  2:564940/3‑100 (MQ=17)


                                                  cacccccAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCcggc                                               <  1:3541273/98‑1 (MQ=17)


                                                  cATCCCCAGGCGCGCGAGAGGCCAGCGCCCTGCCGCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCcggc                                               <  1:1563152/101‑1 (MQ=17)


                                                   aTCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCCGCGGGGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGGG.C.TGGCCGA.GGGCCCTTTGGGGCCCCcgcg                                               >  2:425338/1‑99 (MQ=17)


                                                    cccccAGGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCcggcgg                                             <  2:3761235/100‑1 (MQ=255)


                                                     ccccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGGCGG.GGG.CCCTTGGGGGCCCcggcggg                                            >  1:4404547/1‑100 (MQ=17)


                                                      cccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTc                                           <  1:3068309/101‑1 (MQ=255)


                                                       ccAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.GCCTTGGGGGCCCccgcgggcg                                          >  2:551995/1‑98 (MQ=14)


                                                          aGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.CGGCCGA.GGG.GCCTTTGGGCCCCCGGCGGGCAggg                                       >  2:2102885/1‑101 (MQ=14)


                                                           gcgcgcGAGGGGCCGGCCCCCGGCCCCGGCGGCGCGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgggg                                      <  1:1688815/101‑1 (MQ=14)


                                                           ccgcgcgAGAGCCCACCACCGAGCCCCGGCGCGGCGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAgggg                                      <  2:2971823/100‑1 (MQ=14)


                                                            cgcgcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.GCCTTGGGGGCCCCCGCGGGCCggggg                                     >  2:2374513/1‑101 (MQ=14)


                                                            cacgacagagGCCTGCGCCCCGCCCCGGCGGCGCGA.A.TG.CGG.CCGCCCAAGGCGGGGCGGGGCG.C.AGGCCCG.GGG.CCTTTGGGCCCCCGGGGGGGCCgggc                                      >  2:786682/7‑100 (MQ=14)


                                                             gggcgGGAGGCCAGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                    <  2:1089698/99‑1 (MQ=17)


                                                             gcggggggggCCCGCGCCCCGCCCCGGGGGCGGGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGa                                    <  1:1329072/93‑1 (MQ=14)


                                                                ggagaGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                 <  1:2557067/100‑1 (MQ=255)


                                                                cgAGAGGCCAGCCCCGAGCCCGGGCGGCCCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCt                                 <  2:3557051/101‑1 (MQ=17)


                                                                 gggagGCCGGCCCCCCGCCCCGGCGGGGCAA.A.GC.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  1:4051705/99‑1 (MQ=14)


                                                                 gaggGGCCGGCCCCCGGCCCCGGGGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGT.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  1:4635095/101‑1 (MQ=17)


                                                                  agagGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.CGGCCGAGGGG.CCTTTGGGG.CCCCGGCGGGCAcggggggccgt                               >  2:3424608/1‑95 (MQ=11)


                                                                   ggggCCACCCCCCAGCCCCGGCGGCGCGA.A.CGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  1:3200554/99‑1 (MQ=17)


                                                                   gagGCCGGCCCCCACCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  1:1078351/101‑1 (MQ=17)


                                                                   gagGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  2:2347353/101‑1 (MQ=255)


                                                                    gggCCGGCCCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                             <  1:4275621/100‑1 (MQ=17)


                                                                    agGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGGCAGGGGGGTCTGTTg                             >  1:3326864/1‑101 (MQ=17)


                                                                     gcccAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgc                            <  2:1146790/99‑1 (MQ=255)


                                                                       ccAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTgcgc                          >  1:61318/1‑101 (MQ=255)


                                                                        cAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCa                         >  1:3906125/1‑101 (MQ=255)


                                                                         ggcccccAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  2:3519897/97‑1 (MQ=18)


                                                                           cgcCCAGCCCCGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                      <  2:3191857/101‑1 (MQ=255)


                                                                           cgcCCAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                      >  1:393577/1‑101 (MQ=255)


                                                                           cccccCGCCCGGGCGGCGCGA.A.AGCC.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATgg                      <  1:3270293/99‑1 (MQ=18)


                                                                             cccCGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGcc                    >  1:3141596/1‑101 (MQ=255)


                                                                               cAGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                  >  1:1922812/1‑101 (MQ=255)


                                                                                aGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTGCGCATGGCcgcg                 >  2:3647052/1‑101 (MQ=255)


                                                                                aGCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcg                 >  1:3111582/1‑101 (MQ=255)


                                                                                 gCCCCGGCGGCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGt                >  1:2933209/1‑101 (MQ=255)


                                                                                  ccgaggcggcGGGA.G.AA.C.GCCCGCCATGGGCGGCGC.GGGCG.C.TGGCCGA.GGT.CCTGTGGGGGCCCCGGCGGTCAGGGGCATCTGTTGCGCATGGACGCGTc               <  2:2341654/97‑1 (MQ=18)


                                                                                  cccgGGCGGCGCGA.AGCC.C.C.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc               <  2:906987/101‑1 (MQ=18)


                                                                                  ccccGGCGGCGCGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTc               <  2:1770405/101‑1 (MQ=255)


                                                                                     cggcggCGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGGCAGGGGGATCTGTTGCGCATGGCCGCGTCCgc            >  1:836706/1‑101 (MQ=18)


                                                                                      ggcggcGCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgcg           >  2:214656/1‑101 (MQ=255)


                                                                                         ggcgCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGGTCTGTTGCGCATGGCCGCGTCAGcgccg        >  2:1602591/1‑101 (MQ=255)


                                                                                         ggcgCGA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccg        >  1:2748924/1‑101 (MQ=255)


                                                                                          gcgcGA.A.GG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGcgccgc       <  1:3664769/101‑1 (MQ=255)


                                                                                              gA.A.AG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TTGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc   <  1:1534230/101‑1 (MQ=255)


                                                                                               a.a.aG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa  <  2:1082232/101‑1 (MQ=255)


                                                                                               a.a.aG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa  >  1:84756/1‑101 (MQ=255)


                                                                                               a.a.aG.C.GCCCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa  >  1:1192207/1‑101 (MQ=255)


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCCAAGCGCGCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGA.A.AG.C.G.CCGCCATGGGCGGGGC.GGGCG.C.TGGCCGA.GGG.CCTTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867733‑1867927

Base quality scores:


ATCG

< 3 ≤


ATCG

< 11 ≤


ATCG

< 20 ≤


ATCG

< 31 ≤


ATCG

< 40 ≤


ATCG