Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,925,099 +C 98.1% intergenic (+39/+6) RCAP_rcc01778 → / ← RCAP_rcc01779 hypothetical protein/MazG family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,925,0991.→C98.1% 4.5 ‑4.6 ‑0.4206intergenic (+39/+6)RCAP_rcc01778/RCAP_rcc01779hypothetical protein/MazG family protein
Reads supporting (aligned to +/- strand):  new base (139/63):  ref base (0/4):  total (139/67)
Fisher's exact test strand distribution p-value = 1.05e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.22e-01

Only 100 of 236 total aligned reads displayed.

GCCTCTTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.CC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCAGTTC  >  NC_014034/1924999‑1925194
                                                                                                       |                                                                                                
 ccTCTTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.c                                                                                                 >  1:1812479/1‑101 (MQ=255)
 ccTCTTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.c                                                                                                 >  2:4565619/1‑101 (MQ=255)
   tctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.c                                                                                              <  2:3058765/101‑1 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.ccgcc                                                                                          >  2:3973807/1‑97 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.ccgcc                                                                                          >  1:3941203/1‑97 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.ccgcc                                                                                          >  1:3753850/1‑97 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.ccgcc                                                                                          >  2:2397589/1‑97 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.ccgcc                                                                                          >  1:3028835/1‑97 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.cc.ccgcc                                                                                          >  1:79159/1‑97 (MQ=255)
         cAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTa                                                                                         <  1:3551107/101‑1 (MQ=255)
         cAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTa                                                                                         <  1:555958/101‑1 (MQ=255)
          aGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTAc                                                                                        >  2:3693512/1‑101 (MQ=255)
           gggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACa                                                                                       >  1:3620054/1‑101 (MQ=255)
           gggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACa                                                                                       <  1:244654/101‑1 (MQ=255)
           gggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATACCCCGGGGGGCGGGGCA.GA.GCCC.CGCCTACa                                                                                       <  2:3316753/101‑1 (MQ=38)
           gggTTTCTGATGCTGGTCGCGCTAAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATACCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACa                                                                                       <  1:4225252/101‑1 (MQ=38)
              tttCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGcc                                                                                    >  1:4503530/1‑101 (MQ=255)
                tCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGcccc                                                                                  >  1:2872867/1‑101 (MQ=255)
                 cTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCt                                                                                 >  2:2839769/1‑101 (MQ=255)
                 aagATGCCGATCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCt                                                                                 >  2:1187219/3‑101 (MQ=37)
                  tGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtt                                                                                <  1:591894/101‑1 (MQ=255)
                  tGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtt                                                                                <  2:3832192/101‑1 (MQ=255)
                  tGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtt                                                                                <  2:4677116/101‑1 (MQ=255)
                  tGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtt                                                                                <  2:2287515/101‑1 (MQ=255)
                  tGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtt                                                                                <  2:3094424/101‑1 (MQ=255)
                  tGATGCTGGTCGCGCGTAACATGCTGCGAAAGGCGTTGTTCTGATTTTATCTTGCGGAAAATACCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtt                                                                                <  2:4270795/101‑1 (MQ=25)
                   gATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCtta                                                                               >  1:4677372/1‑100 (MQ=255)
                   gATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTg                                                                               >  1:1877446/1‑101 (MQ=255)
                   caTGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTg                                                                               >  1:1038482/2‑101 (MQ=255)
                       gtGGCCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCTAATACCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCt                                                                           <  2:4107241/100‑1 (MQ=38)
                       cTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCt                                                                           >  1:1552831/1‑101 (MQ=255)
                       cTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCt                                                                           >  2:1033653/1‑101 (MQ=255)
                        tGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCtt                                                                          >  1:4652565/1‑101 (MQ=255)
                         ggTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTg                                                                         >  2:1963418/1‑101 (MQ=255)
                           tCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGc                                                                       >  2:1635856/1‑101 (MQ=255)
                            cgcgCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGcc                                                                      <  2:2156192/101‑1 (MQ=255)
                               gcTGAACATGCTGCGCAAGGCGCTTTTCTGTTTTTATCTTGCGGCAAATACCCGGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCa                                                                   <  1:4418506/101‑1 (MQ=37)
                                cTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCAt                                                                  >  1:1607681/1‑101 (MQ=255)
                                 tGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATc                                                                 >  1:2097187/1‑101 (MQ=255)
                                   aaCATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATccc                                                               >  1:1229942/1‑101 (MQ=255)
                                       tgctgcGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAg                                                           >  1:292644/1‑101 (MQ=255)
                                         ctgcGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAgcg                                                         >  1:1665084/1‑101 (MQ=255)
                                          tgcGCAGGGGGCTGTTCTGTTTTTCTCTTGCGGCAAAGAGCCGGGGCGGGGGGCCA.GACC.CC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAgcgc                                                        <  2:3940961/101‑1 (MQ=255)
                                          tgcGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAgcgc                                                        >  1:3532809/1‑101 (MQ=255)
                                            cgcAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGcgcgc                                                      >  2:1486381/1‑100 (MQ=255)
                                             gcAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTc                                                     >  1:998213/1‑101 (MQ=255)
                                             gcAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATACCAAAGCGCGTc                                                     >  2:4535215/1‑101 (MQ=255)
                                              cagggCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTcc                                                    <  1:3126295/98‑1 (MQ=255)
                                              cAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTcc                                                    >  2:259066/1‑101 (MQ=255)
                                               aaGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCa                                                   <  1:1377866/101‑1 (MQ=255)
                                                aGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCAt                                                  >  2:3495970/1‑101 (MQ=255)
                                                 ggCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCAtc                                                 <  2:585365/101‑1 (MQ=255)
                                                   cgcTGTTCTGATTTTATCTTGCGGCAAATATCCGGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCAtctc                                               <  2:2108628/101‑1 (MQ=255)
                                                    gcTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCg                                              >  1:1499372/1‑101 (MQ=255)
                                                     cTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGGCGCATCCCAAAGCGCGTCCATCTCgg                                             >  1:3984613/1‑101 (MQ=255)
                                                      tGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGc                                            >  2:1845988/1‑101 (MQ=255)
                                                       gTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGcc                                           <  2:264100/101‑1 (MQ=255)
                                                       gTTCGGATTTTATCTTGCGGCAAATATCCGGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGcc                                           <  2:289957/101‑1 (MQ=38)
                                                        ttCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCa                                          >  2:867373/1‑101 (MQ=255)
                                                        ttCTGATTTTATCTTGCGGCAAATATCCCGGGGGGCGGGGCA.AA.G.CCGCGCCTACACCCCCTTGCGCTTGTCCGCATCCCAAAGCGCGTCCATCTNCGCCa                                          >  1:1026098/1‑101 (MQ=38)
                                                           tgtttttATTTGGCGGAAAATACCCGGGGGGGGGGGGCAGGC.C.CC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGAt                                       <  1:3897608/98‑1 (MQ=255)
                                                           tGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGAt                                       <  2:773813/101‑1 (MQ=255)
                                                            gATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATc                                      <  1:1209590/101‑1 (MQ=255)
                                                            gATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATc                                      >  1:4549659/1‑101 (MQ=255)
                                                            gATTTTATCTCGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATc                                      <  2:3562186/101‑1 (MQ=255)
                                                              ttttATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGc                                    >  2:1512897/1‑101 (MQ=255)
                                                              ttttATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGc                                    >  1:3237252/1‑101 (MQ=255)
                                                               tttATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCt                                   >  1:3388119/1‑101 (MQ=255)
                                                               tttATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCt                                   >  1:1748248/1‑101 (MQ=255)
                                                                ttATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTg                                  >  1:2736841/1‑101 (MQ=255)
                                                                 tATCTTGCTGCAAATATCCCGGGGTGCGGGGCA.GG.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGt                                 <  2:3753615/101‑1 (MQ=38)
                                                                    cTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcgg                              >  1:299547/1‑101 (MQ=255)
                                                                      tGCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggcg                            <  1:2208607/101‑1 (MQ=255)
                                                                       gCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggcgg                           >  2:2333722/1‑101 (MQ=255)
                                                                       gCGGCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggcgg                           >  2:3843797/1‑101 (MQ=255)
                                                                         ggCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGc                         >  1:498433/1‑101 (MQ=255)
                                                                          gCAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGcc                        >  2:1612354/1‑101 (MQ=255)
                                                                           cAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGccc                       >  2:1335621/1‑100 (MQ=255)
                                                                           cAAATATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCg                       >  2:1414845/1‑101 (MQ=255)
                                                                            aaaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                      >  1:1155702/1‑101 (MQ=255)
                                                                            aaaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                      >  1:2402760/1‑101 (MQ=255)
                                                                            aaaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                      >  1:689130/1‑101 (MQ=255)
                                                                            aaaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                      >  2:3013303/1‑101 (MQ=255)
                                                                            aaaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                      >  2:3357133/1‑101 (MQ=255)
                                                                             aatcccccGGGGGGGGGGGCAGGC.C.CC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCt                     <  1:4304991/96‑1 (MQ=255)
                                                                             aaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCt                     >  1:1907989/1‑101 (MQ=255)
                                                                             aaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCt                     >  2:4379771/1‑101 (MQ=255)
                                                                             aaTATCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCt                     >  2:2694214/1‑101 (MQ=255)
                                                                              atatCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCtt                    >  1:3474906/1‑101 (MQ=255)
                                                                               tatCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTg                   <  2:4598917/101‑1 (MQ=255)
                                                                                atCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCCTGc                  >  2:1139896/1‑101 (MQ=255)
                                                                                 tCCCGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGcc                 >  2:214340/1‑101 (MQ=255)
                                                                                   ccGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCgg               >  1:881333/1‑101 (MQ=255)
                                                                                   ccGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCgg               >  1:36910/1‑101 (MQ=255)
                                                                                    cGGGGTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGc              >  2:3897096/1‑101 (MQ=255)
                                                                                     ggggTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGcc             >  1:2541357/1‑101 (MQ=255)
                                                                                      gggTGCGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCt            >  1:2294494/1‑101 (MQ=255)
                                                                                        gggCGGGGCA.GACC.CC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTc          <  1:3844032/99‑1 (MQ=255)
                                                                                           cGGGGCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGcc       >  2:198656/1‑101 (MQ=255)
                                                                                             gggCA.GA.GCCC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCAg     >  2:67453/1‑101 (MQ=255)
                                                                                                       |                                                                                                
GCCTCTTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCCGGGGTGCGGGGCA.GA.G.CC.CGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCAGTTC  >  NC_014034/1924999‑1925194

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG