BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,948,851	+G	90.0%	coding (1069/2472 nt)	quiP ←	acyl‑homoserine‑lactone acylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,948,851	1	.→G	90.0%	65.5 ‑1.3 ‑1.1	220	coding (1069/2472 nt)	quiP	acyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand): new base (101/96): ref base (8/14): total (109/111)
Fisher's exact test strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.81e-02

Only 100 of 231 total aligned reads displayed.


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.CCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 >  1:3104249/1‑101 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 >  1:770633/1‑101 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 >  1:1605379/1‑101 (MQ=255)


  aTCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.gg                                                                                                <  2:3630907/101‑2 (MQ=255)


   tCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.ggc                                                                                               >  1:1831893/1‑99 (MQ=255)


    ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcc                                                                                             <  2:395797/101‑1 (MQ=255)


     cGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gccc                                                                                            <  1:1032664/101‑2 (MQ=255)


     cGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gccc                                                                                            <  1:273713/101‑2 (MQ=255)


     cGAAGGCAGAACAGGGCCGTATTCGGACCAGAGCATGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gccc                                                                                            <  1:1525864/101‑2 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATGGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gccca                                                                                           >  2:4108098/1‑99 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaac                                                                                         >  1:3480602/1‑97 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaac                                                                                         <  2:2717244/101‑5 (MQ=255)


        cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaac                                                                                         <  2:770559/101‑5 (MQ=255)


         ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaacc                                                                                        >  1:2819397/1‑96 (MQ=255)


         ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaacc                                                                                        <  1:3969136/101‑6 (MQ=255)


         ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaacc                                                                                        >  2:377088/1‑96 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaaccctc                                                                                     <  1:4493359/101‑9 (MQ=255)


              caCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCgg                                                                                   <  2:3678862/101‑1 (MQ=255)


               aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCggg                                                                                  <  1:63771/101‑1 (MQ=255)


                 cGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGc                                                                                <  2:2359958/101‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCt                                                                               <  2:1491916/101‑1 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCt                                                                               >  1:2023367/1‑101 (MQ=255)


                  gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCt                                                                               >  2:3107640/1‑101 (MQ=255)


                   ggCCGTTTTCGGTCCAGCGCAGGGTGTGGGTGATCGGCGCCGCATCCTTGATTGTGTCGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTg                                                                              <  1:731761/101‑1 (MQ=255)


                   ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTg                                                                              <  2:1133539/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCTTCGGGGCTGc                                                                             <  2:3160453/101‑1 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATTGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTa                                                                         >  2:3338883/1‑101 (MQ=255)


                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTag                                                                        <  1:500038/101‑2 (MQ=255)


                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTag                                                                        >  2:569841/1‑100 (MQ=255)


                         tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTag                                                                        >  2:6609/1‑100 (MQ=255)


                             ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCAGTagcgct                                                                    <  2:1422914/101‑6 (MQ=255)


                                ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGGGAACTCGG.CCC.ACCCCTCGGGGCTGCGGTAGCGCTccg                                                                 >  1:1021557/1‑99 (MQ=255)


                                 cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCggg                                                                >  1:375721/1‑101 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  1:2175879/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:1380249/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:3512330/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:2398035/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  2:3233537/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGgttt                                                          >  2:3455806/1‑101 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  2:1682286/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         >  2:3732618/1‑101 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         >  1:4421073/1‑101 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGCCGCTCACCTTGCGGGTGGTGAATTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        <  1:4056159/101‑1 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        >  1:3334857/1‑101 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        <  1:1977764/101‑1 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       >  1:1937766/1‑101 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    <  1:2270727/101‑1 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  1:1662649/1‑101 (MQ=255)


                                                  gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  1:4468808/1‑101 (MQ=255)


                                                    tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                             >  1:1235842/1‑101 (MQ=255)


                                                     cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            <  2:1927608/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGAGGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:3227378/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          >  1:1783472/1‑101 (MQ=255)


                                                        gtcgccATCCTTTGCCGTGGCGGTCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  1:4198741/96‑1 (MQ=255)


                                                        cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  2:1068447/101‑1 (MQ=255)


                                                        atctgCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  2:3534977/97‑1 (MQ=255)


                                                         gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                        >  1:3902402/1‑101 (MQ=255)


                                                          cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                       <  2:1208755/101‑1 (MQ=255)


                                                           ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                      >  1:1867692/1‑101 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  2:2553955/1‑101 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:3002796/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:3395084/101‑1 (MQ=255)


                                                               tCCTTGACCTTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:2779428/101‑1 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:1350889/101‑1 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GACCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:1831784/101‑1 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                >  2:2477878/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:4054578/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:3726929/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  1:2613207/1‑101 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:2627338/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:54850/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:2554107/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGCCGAACAGGTCCTc                            >  2:1471397/1‑101 (MQ=255)


                                                                      ccGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                           >  2:4169164/1‑101 (MQ=255)


                                                                      ccGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                           <  1:1304854/101‑1 (MQ=255)


                                                                      ccGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                           <  2:2897405/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         >  1:2189077/1‑101 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:2312077/101‑1 (MQ=255)


                                                                        gTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:4260655/101‑1 (MQ=255)


                                                                         tGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        >  1:892556/1‑101 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:2665780/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:4609481/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGCGGTGAACTC.GTCCCAA.CCCTCGGGGCTGCGGTATCGCTCCTGCATTTTCGGAGCATGTTCCACGAGGACCAGGTCCTCGTCAt                       >  2:486186/1‑101 (MQ=255)


                                                                           aCGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                      <  1:2677903/101‑1 (MQ=255)


                                                                            cGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                     <  2:1897321/101‑1 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  2:4396819/101‑1 (MQ=255)


                                                                                cACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  1:3727488/1‑101 (MQ=255)


                                                                                cACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  2:2205567/1‑101 (MQ=255)


                                                                                  ccTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAg               >  2:43323/1‑101 (MQ=255)


                                                                                      gCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           >  2:385033/1‑101 (MQ=255)


                                                                                      gCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  1:2759851/101‑1 (MQ=255)


                                                                                      gCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           >  1:215763/1‑101 (MQ=255)


                                                                                        gggTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg         >  1:2071570/1‑101 (MQ=255)


                                                                                           tggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      <  1:3940732/101‑1 (MQ=255)


                                                                                           tggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      >  1:1261174/1‑101 (MQ=255)


                                                                                            ggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     <  2:1783363/101‑1 (MQ=255)


                                                                                            ggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGa     >  1:1478433/1‑101 (MQ=255)


                                                                                             gtgAACTCAG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGTCGGCGGTGAt    <  1:4101816/101‑1 (MQ=255)


                                                                                               gAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  2:4468558/101‑1 (MQ=255)


                                                                                               gAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc  >  2:3093230/1‑101 (MQ=255)


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.CCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG