Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,855 +A 100% coding (1065/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8551.→A100.0% 775.2 200coding (1065/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (101/97):  ref base (0/2):  total (101/99)

Only 100 of 222 total aligned reads displayed.

CCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.CCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948755‑1948943
                                                                                                       |                                                                                          
ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcc                                                                                             <  2:395797/101‑1 (MQ=255)
 cGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gccc                                                                                            <  1:1032664/101‑2 (MQ=255)
  gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATGGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gccca                                                                                           >  2:4108098/1‑99 (MQ=255)
  gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCTCCTTGCGGGTGGTGAACTC.G.gccca                                                                                           <  1:746290/101‑3 (MQ=255)
    cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaac                                                                                         <  2:770559/101‑5 (MQ=255)
    cGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaac                                                                                         >  1:3480602/1‑97 (MQ=255)
     ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaacc                                                                                        >  2:377088/1‑96 (MQ=255)
     ggCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaacc                                                                                        >  1:2819397/1‑96 (MQ=255)
        agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.gcccaaccctc                                                                                     <  1:4493359/101‑9 (MQ=255)
         gcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCg                                                                                    <  1:2087731/101‑1 (MQ=255)
          caCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCgg                                                                                   <  2:3678862/101‑1 (MQ=255)
           aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCggg                                                                                  <  1:63771/101‑1 (MQ=255)
           aCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCggg                                                                                  <  2:3104049/101‑1 (MQ=255)
              gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCt                                                                               <  1:1264215/101‑1 (MQ=255)
              gggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCt                                                                               <  2:1491916/101‑1 (MQ=255)
               ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTg                                                                              <  2:1133539/101‑1 (MQ=255)
                gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGc                                                                             <  2:2099703/101‑1 (MQ=255)
                   gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGt                                                                          >  2:2647987/1‑101 (MQ=255)
                   gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CACTCGGGGCTGCGGt                                                                          >  2:532335/1‑101 (MQ=255)
                    ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTa                                                                         <  1:3080486/101‑1 (MQ=255)
                     tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTag                                                                        >  2:1643017/1‑100 (MQ=255)
                     tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTag                                                                        >  2:569841/1‑100 (MQ=255)
                     tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTag                                                                        >  2:6609/1‑100 (MQ=255)
                      ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCTGCATCCTTGACCGTGACGATCGCCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTagc                                                                       <  2:3118474/101‑3 (MQ=255)
                      ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTagc                                                                       <  1:3228849/101‑3 (MQ=255)
                      ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTagc                                                                       >  1:2665934/1‑99 (MQ=255)
                          gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTc                                                                   <  2:3452243/101‑1 (MQ=255)
                            ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGGGAACTCGG.CCC.ACCCCTCGGGGCTGCGGTAGCGCTccg                                                                 >  1:1021557/1‑99 (MQ=255)
                             cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCggg                                                                >  1:375721/1‑101 (MQ=255)
                                 gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  1:2175879/101‑1 (MQ=255)
                                  cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           >  1:2015649/1‑101 (MQ=255)
                                   agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCCCCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:1870053/1‑101 (MQ=255)
                                   agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:2398035/101‑1 (MQ=255)
                                   agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:1380249/101‑1 (MQ=255)
                                    gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         >  2:3732618/1‑101 (MQ=255)
                                    gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         >  1:4421073/1‑101 (MQ=255)
                                     ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGCCGCTCACCTTGCGGGTGGTGAATTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        <  1:4056159/101‑1 (MQ=255)
                                     ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        >  1:3334857/1‑101 (MQ=255)
                                      gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       >  2:3582800/1‑101 (MQ=255)
                                      gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       >  1:1937766/1‑101 (MQ=255)
                                        gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                     <  2:4399074/101‑1 (MQ=255)
                                         agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  2:349133/1‑101 (MQ=255)
                                         agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  2:3918124/1‑101 (MQ=255)
                                         agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    <  1:2270727/101‑1 (MQ=255)
                                            gtgaTCGGCGCGGCATCCTTGACCGTGAGGCTCACCTTGCGGGTGGTAAACTCGG.CCC.ACCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 <  2:1867575/101‑1 (MQ=255)
                                            gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                 >  1:72112/1‑101 (MQ=255)
                                             tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                <  2:3267355/101‑1 (MQ=255)
                                             tgaTCGGCGCGGCATCCTTGAACGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                >  1:2699283/1‑101 (MQ=255)
                                              gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  1:1662649/1‑101 (MQ=255)
                                              gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                               >  1:4468808/1‑101 (MQ=255)
                                                tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                             >  1:1235842/1‑101 (MQ=255)
                                                 cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            >  2:298498/1‑101 (MQ=255)
                                                 cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                            <  2:2423046/101‑1 (MQ=255)
                                                   gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          >  1:1783472/1‑101 (MQ=255)
                                                    gtcgccATCCTTTGCCGTGGCGGTCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  1:4198741/96‑1 (MQ=255)
                                                    cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                         <  2:1068447/101‑1 (MQ=255)
                                                      cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                       <  2:1208755/101‑1 (MQ=255)
                                                       ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                      >  1:1569689/1‑101 (MQ=255)
                                                          aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:3395084/101‑1 (MQ=255)
                                                           tCCTTGACCTTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:2779428/101‑1 (MQ=255)
                                                           tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  1:472144/1‑101 (MQ=255)
                                                           tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:1350889/101‑1 (MQ=255)
                                                           tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GACCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  <  2:1831784/101‑1 (MQ=255)
                                                             cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAggc                                >  1:397987/1‑100 (MQ=255)
                                                             cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                >  1:3871459/1‑101 (MQ=255)
                                                              ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:4054578/1‑101 (MQ=255)
                                                              ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  1:2613207/1‑101 (MQ=255)
                                                              ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               >  2:3726929/1‑101 (MQ=255)
                                                               tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                              <  1:1853694/101‑1 (MQ=255)
                                                                gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:54850/101‑1 (MQ=255)
                                                                gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:2627338/101‑1 (MQ=255)
                                                                gACCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  1:2554107/101‑1 (MQ=255)
                                                                 aCCGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGCCGAACAGGTCCTc                            >  2:1471397/1‑101 (MQ=255)
                                                                  ccGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                           >  2:4169164/1‑101 (MQ=255)
                                                                  ccGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                           <  2:2897405/101‑1 (MQ=255)
                                                                   cGTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          <  1:3909937/101‑1 (MQ=255)
                                                                    gTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:1494211/101‑1 (MQ=255)
                                                                    gTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:4260655/101‑1 (MQ=255)
                                                                    gTGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                         <  1:2312077/101‑1 (MQ=255)
                                                                     tGACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        >  1:892556/1‑101 (MQ=255)
                                                                      gACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  2:4609481/101‑1 (MQ=255)
                                                                      gACGATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       <  1:2070546/101‑1 (MQ=255)
                                                                         gATCACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  2:2534848/1‑101 (MQ=255)
                                                                            cACCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                 >  1:3727488/1‑101 (MQ=255)
                                                                             aCCTTGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                >  1:2216921/1‑101 (MQ=255)
                                                                                ttGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggg             >  2:2694137/1‑100 (MQ=255)
                                                                                 tGCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAGCAGGTCCTCGTCATCGGCGAAggcg            >  1:2995861/1‑101 (MQ=255)
                                                                                  gCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           >  1:215763/1‑101 (MQ=255)
                                                                                  gCGGGTGGTGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg           <  1:2759851/101‑1 (MQ=255)
                                                                                    gggTGGTGAACTCGG.CCCAA.CGCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg         >  1:3500038/1‑101 (MQ=255)
                                                                                     ggtggtGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg        >  2:4334483/1‑101 (MQ=255)
                                                                                     ggtggtGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg        >  2:320571/1‑101 (MQ=255)
                                                                                     ggtggtGAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg        <  2:2220789/101‑1 (MQ=255)
                                                                                       tggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      >  2:1722422/1‑101 (MQ=255)
                                                                                       tggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg      >  1:1261174/1‑101 (MQ=255)
                                                                                        ggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  2:1910983/1‑101 (MQ=255)
                                                                                        ggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa     >  2:1376152/1‑101 (MQ=255)
                                                                                        ggtgAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGa     >  1:1478433/1‑101 (MQ=255)
                                                                                         gtgAACTCAG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGTCGGCGGTGAt    <  1:4101816/101‑1 (MQ=255)
                                                                                           gAACTCGG.CCCAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  <  2:4468558/101‑1 (MQ=255)
                                                                                                       |                                                                                          
CCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.G.CCC.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948755‑1948943

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG