Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,873 +G 100% coding (1047/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8731.→G100.0% 774.3 203coding (1047/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (104/98):  ref base (1/0):  total (105/98)

Only 100 of 223 total aligned reads displayed.

TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.A.CCCTCGGGGCTGCGG.TA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967
                                                                                                       |                                                                                              
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.Ta                                                                                                 <  1:3080486/101‑1 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.Tag                                                                                                >  2:1643017/1‑100 (MQ=255)
  ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.Tagc                                                                                               >  1:2665934/1‑99 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.Tagcg                                                                                              <  1:278145/101‑4 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTc                                                                                           <  2:3452243/101‑1 (MQ=255)
       tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCg                                                                                          >  1:1336514/1‑101 (MQ=255)
        ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCgg                                                                                         >  1:1262228/1‑101 (MQ=255)
        ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGGGAACTCGGCCC.ACCCCTCGGGGCTGCGG.TAGCGCTccg                                                                                         >  1:1021557/1‑99 (MQ=255)
         cAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCggg                                                                                        >  1:375721/1‑101 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGtt                                                                                    <  1:2175879/101‑1 (MQ=255)
              cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGttt                                                                                   <  1:167087/101‑1 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCCCCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGtttt                                                                                  >  1:1870053/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGtttt                                                                                  <  1:2398035/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTc                                                                                 >  2:3732618/1‑101 (MQ=255)
                 ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGCCGCTCACCTTGCGGGTGGTGAATTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCg                                                                                <  1:4056159/101‑1 (MQ=255)
                 ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCg                                                                                <  1:1977764/101‑1 (MQ=255)
                 ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCg                                                                                >  1:3334857/1‑101 (MQ=255)
                 ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCg                                                                                <  2:4126606/101‑1 (MQ=255)
                  gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCgg                                                                               >  2:3582800/1‑101 (MQ=255)
                    gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGAt                                                                             >  1:1939131/1‑101 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATc                                                                            >  2:3918124/1‑101 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATc                                                                            <  1:2270727/101‑1 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATc                                                                            >  2:349133/1‑101 (MQ=255)
                       ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCaa                                                                          <  2:1443340/101‑1 (MQ=255)
                        gtgaTCGGCGCGGCATCCTTGACCGTGAGGCTCACCTTGCGGGTGGTAAACTCGGCCC.ACCCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAg                                                                         <  2:1867575/101‑1 (MQ=255)
                        gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAg                                                                         >  1:72112/1‑101 (MQ=255)
                         tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGt                                                                        <  2:3267355/101‑1 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGGGCTCGGGCGTTTTCGGATCAAGtt                                                                       >  2:605091/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                       >  1:4468808/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGGGGGGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCTGGCGTTTTCGGATCAAGtt                                                                       >  2:2904337/1‑101 (MQ=255)
                           aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTc                                                                      <  1:4404515/101‑1 (MQ=255)
                            tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                                                     >  1:1235842/1‑101 (MQ=255)
                            tCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTcc                                                                     >  1:3980183/1‑101 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                    <  2:1927608/101‑1 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                    <  1:3348652/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGAGGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  1:3227378/101‑1 (MQ=255)
                               gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                                                  >  1:1783472/1‑101 (MQ=255)
                                cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                                                 <  2:1068447/101‑1 (MQ=255)
                                atctgCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcga                                                                 <  2:3534977/97‑1 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                                >  1:3902402/1‑101 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                               <  1:3428978/101‑1 (MQ=255)
                                    gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                                             >  2:2553955/1‑101 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTGGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  1:4234154/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           <  2:3190038/101‑1 (MQ=255)
                                       tCCTTGACCTTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                          <  2:2779428/101‑1 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                          >  1:472144/1‑101 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                        <  1:1660895/101‑1 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                       >  1:2613207/1‑101 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                       >  2:3726929/1‑101 (MQ=255)
                                          ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                                                       >  2:4054578/1‑101 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      <  1:1853694/101‑1 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                     <  1:2554107/101‑1 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                     <  1:54850/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                    >  2:476995/1‑101 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                    <  2:1489186/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACTAACAGGTCCTCGTc                                                 >  1:1439965/1‑101 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                 <  1:1494211/101‑1 (MQ=255)
                                                gTGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTc                                                 <  1:2312077/101‑1 (MQ=255)
                                                 tGACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                                                >  1:892556/1‑101 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               <  2:2665780/101‑1 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               <  2:4609481/101‑1 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                            >  2:1824174/1‑101 (MQ=255)
                                                       tCACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                                          <  2:4396819/101‑1 (MQ=255)
                                                        cACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                                         >  1:3727488/1‑101 (MQ=255)
                                                        cACCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.CAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGa                                         >  2:118171/1‑101 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                        >  1:2216921/1‑101 (MQ=255)
                                                             tGCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAGCAGGTCCTCGTCATCGGCGAAggcg                                    >  1:2995861/1‑101 (MQ=255)
                                                              gCGGGTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg                                   >  1:215763/1‑101 (MQ=255)
                                                                gggTGGTGAACTCGGCCCAA.CGCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                 >  1:3500038/1‑101 (MQ=255)
                                                                gggTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                 >  1:2071570/1‑101 (MQ=255)
                                                                gggTGGTGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                 <  1:4282361/101‑1 (MQ=255)
                                                                 ggtggtGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg                                <  2:2220789/101‑1 (MQ=255)
                                                                 ggtggtGAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg                                >  2:320571/1‑101 (MQ=255)
                                                                   tggtgAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTg                              >  2:1722422/1‑101 (MQ=255)
                                                                    ggtgAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                             >  2:1376152/1‑101 (MQ=255)
                                                                     gtgAACTCAGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGTCGGCGGTGAt                            <  1:4101816/101‑1 (MQ=255)
                                                                       gAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc                          <  2:4468558/101‑1 (MQ=255)
                                                                       gAACTCGGCCCAA.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc                          >  2:3093230/1‑101 (MQ=255)
                                                                         actcggcccaa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGccc                        <  2:3824319/91‑1 (MQ=255)
                                                                          ctcggcccaa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGcccc                       <  1:377132/92‑1 (MQ=255)
                                                                           ccggcccaa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCa                      <  2:909594/93‑1 (MQ=255)
                                                                             ggcccaa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGc                    >  1:4198015/7‑101 (MQ=255)
                                                                              gcccaa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                   <  1:569892/96‑1 (MQ=255)
                                                                                 caa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAg                >  2:1392334/3‑101 (MQ=255)
                                                                                  aa.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGCCGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGc               >  2:3980560/2‑101 (MQ=255)
                                                                                   a.CCCTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCt              >  1:1901877/1‑101 (MQ=255)
                                                                                     cccTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTg             >  2:4230921/1‑101 (MQ=255)
                                                                                      ccacGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATC.GCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGt           <  1:2076525/98‑1 (MQ=255)
                                                                                      ccTCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTgg            >  1:3943445/1‑101 (MQ=255)
                                                                                       cTCGGGGCTGCGGCCG.CGCTGGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGACGGCGGCGGTGGTGCCCCAGCCAAGATGGt           >  2:424565/1‑101 (MQ=255)
                                                                                        tCGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTc          <  1:3571538/101‑1 (MQ=255)
                                                                                         cGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg         <  2:2015532/101‑1 (MQ=255)
                                                                                         cGGGGCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCg         >  2:830563/1‑101 (MQ=255)
                                                                                          ggggCTGCGG.TAGCGCTCGGGCGTTTTCGGGTCTAGTTCCTCGACGAGCAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        <  2:1869941/101‑1 (MQ=255)
                                                                                          ggggCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        <  2:2416335/101‑1 (MQ=255)
                                                                                           gggCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCt       >  1:2686810/1‑101 (MQ=255)
                                                                                            ggCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTg      >  1:668398/1‑101 (MQ=255)
                                                                                             gCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc     <  1:27148/101‑1 (MQ=255)
                                                                                             gCTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgc     <  2:683734/101‑1 (MQ=255)
                                                                                              cTGCGG.TAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    >  2:2510306/1‑101 (MQ=255)
                                                                                                       |                                                                                              
TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GCCC.A.CCCTCGGGGCTGCGG.TA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG