BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,974,330	+G	100%	intergenic (‑23/+32)	rpe1 ← / ← cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,974,330	1	.→G	100.0%	728.9	196	intergenic (‑23/+32)	rpe1/cbbM	ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand): new base (102/92): ref base (1/1): total (103/93)

Only 100 of 220 total aligned reads displayed.


ATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GGC..G.GG.GTGT.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTC  >  NC_014034/1974234‑1974427


aTTTCACCGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GGA..g.gg.g                                                                                                 >  2:4075665/1‑101 (MQ=255)


 tttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..g.gg.g                                                                                                 >  2:2114351/1‑101 (MQ=255)


  ttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGCGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..g.gg.gg                                                                                                >  1:2058873/1‑100 (MQ=255)


  ttCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..g.gg.gg                                                                                                <  1:3925372/101‑2 (MQ=255)


        gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGT.c.cc                                                                                         <  1:1151348/101‑1 (MQ=255)


         ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGT.c.ccc                                                                                        <  2:1697404/101‑1 (MQ=255)


           gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGT.c.ccccc                                                                                      <  1:2848651/101‑2 (MQ=255)


            aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGCGGGGTTGG.GGCG.G.GG.GTGT.c.gccccc                                                                                     >  2:1132057/1‑99 (MQ=255)


            aaaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGT.c.cccccg                                                                                     >  1:1612898/1‑99 (MQ=255)


             aaTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..GTGG.GTGT.c.cccccgc                                                                                    >  2:1327463/1‑98 (MQ=255)


               tCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGGTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGT.c.cccccgccc                                                                                  <  1:4068101/101‑6 (MQ=255)


                cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.Ccccgcccg                                                                                  >  2:461339/1‑101 (MQ=255)


                 ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCgg                                                                                 <  2:1156910/101‑1 (MQ=255)


                 ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCgg                                                                                 <  1:1030027/101‑1 (MQ=255)


                    gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATa                                                                              <  2:4308822/101‑1 (MQ=255)


                    gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATa                                                                              <  1:1206509/101‑1 (MQ=255)


                     aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAg                                                                             >  2:2712428/1‑101 (MQ=255)


                     aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAg                                                                             <  2:665590/101‑1 (MQ=255)


                     aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAg                                                                             >  2:4429666/1‑101 (MQ=255)


                      aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGc                                                                            <  1:1484613/101‑1 (MQ=255)


                       gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCa                                                                           <  2:365848/101‑1 (MQ=255)


                        tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCAc                                                                          <  2:3840137/101‑1 (MQ=255)


                        cgatctgAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCAc                                                                          <  2:1402427/95‑1 (MQ=255)


                           gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCAcggc                                                                       <  2:754018/101‑4 (MQ=255)


                            cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAGCACCgcg                                                                      >  2:1616741/1‑99 (MQ=255)


                             aGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGGTAGCA.CGGCGt                                                                    >  1:3354033/1‑101 (MQ=255)


                              gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCAcggcggt                                                                    >  2:3299496/1‑95 (MQ=255)


                              gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAGCagggcgtt                                                                    >  2:2170251/1‑94 (MQ=255)


                               aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                                  <  1:1939146/101‑1 (MQ=255)


                               aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                                  <  1:3752744/101‑1 (MQ=255)


                                aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGT.CGCCCCGCCCGGATAGCA.CGGCGTTCa                                                                 >  2:4706456/1‑101 (MQ=255)


                                aaGGATCGAGGGAGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                                 >  1:2565757/1‑101 (MQ=255)


                                 aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                                >  1:4278273/1‑101 (MQ=255)


                                   gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGC..G.GGCGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                              <  1:4725300/101‑1 (MQ=255)


                                     ctgAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                            <  1:1828011/99‑3 (MQ=255)


                                         gggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGcc                                                        >  1:2779189/1‑101 (MQ=255)


                                          ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                       <  1:2419945/101‑1 (MQ=255)


                                          ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                       <  1:3446337/101‑1 (MQ=255)


                                          ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                       <  1:4577056/101‑1 (MQ=255)


                                            ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                     <  2:464022/101‑1 (MQ=255)


                                            ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                     <  2:745666/101‑1 (MQ=255)


                                            ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                     <  1:4495499/101‑1 (MQ=255)


                                             gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                    <  2:4180979/101‑1 (MQ=255)


                                             gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                    <  1:3383448/101‑1 (MQ=255)


                                             gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                    <  2:1116362/101‑1 (MQ=255)


                                              cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCatag                                                   <  1:2712582/101‑1 (MQ=255)


                                              cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                   >  2:352117/1‑101 (MQ=255)


                                               gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                  >  2:47879/1‑101 (MQ=255)


                                                aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                 <  1:1967036/101‑1 (MQ=255)


                                                aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                 <  1:2590427/101‑1 (MQ=255)


                                                 tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                                <  2:25192/101‑1 (MQ=255)


                                                 tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                                >  2:120742/1‑101 (MQ=255)


                                                   ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                              <  2:2058752/101‑1 (MQ=255)


                                                   ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                              >  1:4377194/1‑101 (MQ=255)


                                                    tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                             >  2:4487067/1‑101 (MQ=255)


                                                      aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                           >  1:3522086/1‑101 (MQ=255)


                                                       tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                          >  1:2562052/1‑101 (MQ=255)


                                                       tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                          >  1:993378/1‑101 (MQ=255)


                                                        ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                         <  2:1795367/101‑1 (MQ=255)


                                                        ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                         <  1:669250/101‑1 (MQ=255)


                                                          aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                                       >  2:2844603/1‑101 (MQ=255)


                                                          aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                                       >  1:1368237/1‑101 (MQ=255)


                                                            cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                     >  2:2880545/1‑101 (MQ=255)


                                                             ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCGGG.GG.GGGC.C.CCCCGCCCGGGTAGCA.CGGGGGTCAGTTCACGCCCCGAGCGACGCGCCAGCCCg                                    >  1:1659009/1‑101 (MQ=255)


                                                             ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                    >  2:3139942/1‑101 (MQ=255)


                                                             ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                    <  1:1492779/101‑1 (MQ=255)


                                                              gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccccg                                   >  2:1685665/1‑99 (MQ=255)


                                                              gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                   >  2:1438702/1‑101 (MQ=255)


                                                              gTCGAAGGTCATGTCGGGCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                   <  2:1487065/101‑1 (MQ=255)


                                                                cGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                 <  1:528055/101‑1 (MQ=255)


                                                                cGAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCAGAt                                 >  2:1195079/1‑101 (MQ=255)


                                                                 gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                >  2:4061073/1‑101 (MQ=255)


                                                                 gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                <  1:1346452/101‑1 (MQ=255)


                                                                 gAAGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                >  2:2246729/1‑101 (MQ=255)


                                                                  aaGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                               >  1:935864/1‑101 (MQ=255)


                                                                  aaGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                               >  2:1419532/1‑101 (MQ=255)


                                                                  aaGGTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                               <  2:3813082/101‑1 (MQ=255)


                                                                    ggTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATagag                             >  1:4467117/1‑101 (MQ=255)


                                                                     gTCATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                            <  1:2478146/101‑1 (MQ=255)


                                                                       cATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                          >  1:3272531/1‑101 (MQ=255)


                                                                       cATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                          >  2:614408/1‑101 (MQ=255)


                                                                       cATGTCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                          <  1:561190/101‑1 (MQ=255)


                                                                           tCGGTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                      >  2:1596399/1‑101 (MQ=255)


                                                                             ggTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc                    <  2:1358684/101‑1 (MQ=255)


                                                                              gTCTCCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCAGATAGAGCTTGTCGGCa                   >  2:4266103/1‑101 (MQ=255)


                                                                                ctcCTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                 <  1:2011974/101‑1 (MQ=255)


                                                                                  ccTGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt               >  2:390944/1‑101 (MQ=255)


                                                                                    tGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc             >  1:4625674/1‑101 (MQ=255)


                                                                                    tGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc             >  2:2128620/1‑101 (MQ=255)


                                                                                    tGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc             >  1:3100479/1‑101 (MQ=255)


                                                                                    tGAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTCCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc             <  1:2198446/101‑1 (MQ=255)


                                                                                    tGAGGGGTT.GAGGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc             >  1:4104589/1‑101 (MQ=255)


                                                                                     ggggggTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg            <  1:3970560/99‑1 (MQ=255)


                                                                                     gAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg            <  2:342137/101‑1 (MQ=255)


                                                                                     gAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg            <  1:579309/101‑1 (MQ=255)


                                                                                     gAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg            <  1:1685876/101‑1 (MQ=255)


                                                                                     gAGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg            >  1:2810832/1‑101 (MQ=255)


                                                                                      gggggTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGGCGAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg           <  2:3228921/100‑1 (MQ=255)


                                                                                      aGGGGTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg           >  1:3523034/1‑101 (MQ=255)


                                                                                       ggggTTGG.GGCG.G.GG.GTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg          <  2:2932784/101‑1 (MQ=255)


ATTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.G.GGC..G.GG.GTGT.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTC  >  NC_014034/1974234‑1974427

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG