Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,979,921 +G 2.0% intergenic (‑37/+47) tkt1 ← / ← cbbP transketolase/phosphoribulokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,979,9211.→G 2.0% 4.6 ‑2.1 ‑0.2196intergenic (‑37/+47)tkt1/cbbPtransketolase/phosphoribulokinase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (97/95):  total (101/95)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.75e-01

Only 100 of 230 total aligned reads displayed.

CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021
                                                                                                     |                                                                                                    
ccatcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAgcggc                                                                                                       >  1:1743353/1‑101 (MQ=255)
ccatcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAgcggc                                                                                                       >  1:1921305/1‑101 (MQ=255)
 catcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAgcggc.g                                                                                                     <  1:3633770/101‑1 (MQ=255)
  atcGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGcggc.gg                                                                                                    >  1:1410366/1‑101 (MQ=255)
    cGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGc                                                                                                  <  2:2121574/101‑1 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGgcc                                                                                                >  1:3880887/1‑101 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGccc                                                                                                <  2:481179/101‑1 (MQ=255)
      ccAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGccc                                                                                                <  2:4092648/101‑1 (MQ=255)
          cgcCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTg                                                                                            <  1:2026818/101‑1 (MQ=255)
              cGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTgggcc                                                                                        <  2:3460215/101‑1 (MQ=255)
              cGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGgggcc                                                                                        >  2:3854661/1‑101 (MQ=255)
                gATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCCGGgggccgg                                                                                      >  1:540115/1‑101 (MQ=255)
                   ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTgggccgggcc                                                                                   <  2:1293825/101‑1 (MQ=255)
                   ggCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTgggccgggcc                                                                                   <  2:4683412/101‑1 (MQ=255)
                    gCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCGGTgggccgggccc                                                                                  >  2:40105/1‑100 (MQ=255)
                    gCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCt                                                                                  <  2:844437/101‑1 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTc                                                                                 <  1:879582/101‑1 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTc                                                                                 <  1:4331450/101‑1 (MQ=255)
                     cATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTc                                                                                 <  2:2664622/101‑1 (MQ=255)
                      gttgcggaggcgcgTTTCCTGCGCCATCTCGAGTTCATTCATGGCTCCTCCTCCCTGAGCAGCTTTTTGTTGGAGCGGC.GGGCCCGGTGGGCCGGGCCTCg                                                                                <  2:3537286/91‑1 (MQ=255)
                      aTTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCGGCggggcggcgctcc                                                                                >  1:1498564/1‑95 (MQ=255)
                      aTTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCg                                                                                <  2:3670993/101‑1 (MQ=255)
                       ttGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGCGGGGCCCGGGGGGCCGGGCGTcc                                                                                >  1:286105/1‑100 (MQ=255)
                        tGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGggggcgggccccccc                                                                              >  2:1069282/1‑96 (MQ=255)
                         ggCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCa                                                                             <  1:4176923/101‑1 (MQ=255)
                          gCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCaa                                                                            <  1:1547293/101‑1 (MQ=255)
                           ccATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAAt                                                                           <  1:1878484/101‑1 (MQ=255)
                            cATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATg                                                                          <  1:361053/101‑1 (MQ=255)
                               ccGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCt                                                                       <  1:4541825/101‑1 (MQ=255)
                                 gcttttCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTg                                                                     <  2:1665167/98‑1 (MQ=255)
                                 gcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTg                                                                     <  2:4325522/101‑1 (MQ=255)
                                 gcgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGTGGGCCGGGCCCCGCCCATGTCttt                                                                     >  2:659231/1‑100 (MQ=255)
                                  cgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGa                                                                    <  2:3791445/101‑1 (MQ=255)
                                  cgTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGGGGGCGGGCCCCGCCCAAGTCttta                                                                    >  1:4039881/1‑99 (MQ=255)
                                   gTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGGCCCGCCCATGTCTTTaa                                                                   >  2:2645739/1‑101 (MQ=255)
                                       ccTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGCGTGCCGGGCCCCGCCCATGTCtttgcatct                                                               >  2:2882926/1‑94 (MQ=255)
                                           cgcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGGCGGGCCCCGCCCATGTCTTTAAATCGtttc                                                           >  2:2584500/1‑99 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCg                                                          <  1:1313497/101‑1 (MQ=255)
                                            gcCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGGCGGGGCCCGCCCATTTCTTGAAAATGTTCCg                                                          >  1:4747368/1‑101 (MQ=255)
                                            gcCATAGCGAGATCCTTGATGGCTCCACCTACCTGATAAGCCTTTCATCCGAGCGGC.GGGCCCGGTGGTCCGGGCCTCGCCAATGTCTTGAAATCGTTCCg                                                          <  2:3918727/101‑1 (MQ=255)
                                              cATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCCGGGGGGCGGGCCGCCCCCATGTCTTTCAAATGTTCCggg                                                        >  2:2173880/1‑100 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCCGGGGGGCGGGGCTCCCCCATGTGTTGGACTGCTTCCgggt                                                       >  2:1092958/1‑99 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGtt                                                       <  1:4256818/101‑1 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGGCGGGCCCCGCCCATGTCTTTGAATCGTTcccggt                                                       >  1:220950/1‑97 (MQ=255)
                                               atatCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGAGAGGCGGGCCGCGCCCATGTCTTGAAAACAGTCCGGtt                                                       >  1:742804/1‑101 (MQ=255)
                                               atatCGAGATCCTTCATGGCCCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGGGGCCAGGCCTCGCCCATGCGTTGACAACGTTCCGGtt                                                       >  2:319258/1‑101 (MQ=255)
                                                 atCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGGCGGGGCCCGCCCATGTCTTTAAAACGGTCCGGGTCa                                                     >  2:4197947/1‑101 (MQ=255)
                                                   cGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGTGTGTGGGGCCCCGCCAACGTTTTGAAATCCTTCCGGGTCAgg                                                   >  2:4476156/1‑101 (MQ=255)
                                                   cGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGGGGGCGGGCCTCCCCCAAGTCTTTAAAACGTTCCgggttcgg                                                   >  1:380491/1‑95 (MQ=255)
                                                    gagaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCGGTGGGGCGGGGCCCGCCAATTGCTTGAAATTGTTCCGGGTCAGGc                                                  >  2:603797/1‑101 (MQ=255)
                                                    gagaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGGCCCGCCCATGTCTTGAAATCGTTCCCGGTCTGGc                                                  >  1:2491924/1‑101 (MQ=255)
                                                      gaTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGGCTCCCCCATGTCTTTAAATCGTTCCGGTTCCGGccc                                                >  1:4125658/1‑101 (MQ=255)
                                                       aTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcg                                               <  2:4163780/101‑1 (MQ=255)
                                                       aTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcg                                               <  1:1141107/101‑1 (MQ=255)
                                                       aTCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGGGGGCCGGGCCCCGCCCATGTCTTTAAATCGGTCCGGTTCAGGcccc                                               >  1:2430056/1‑100 (MQ=255)
                                                            tCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCGGTGGGCCGGGGCTCGCCAATGTATTGAAATCGTTTCGGTTTCGGCCcgcccgg                                          >  2:2009431/1‑100 (MQ=255)
                                                            tCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGGCGGGGCTCGCCAATGTCTTGAAATCGTTCCCGTTCACGCCcgccccc                                          >  1:338320/1‑97 (MQ=255)
                                                            tCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGGCGGGCCTCGCCCATGTCTTTAAATTGTTCCGGTTCAGGCCcgcgccg                                          >  1:4656489/1‑99 (MQ=255)
                                                             cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcgcgctcg                                         <  1:2707429/101‑1 (MQ=255)
                                                             cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCcgcgcgcg                                         <  1:3562008/101‑1 (MQ=255)
                                                             cATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGCCTCGCCAATGTCTTTAAATCGTTCCCGTTCAGGCCcgcgcgcg                                         >  1:657753/1‑101 (MQ=255)
                                                              aTGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGGCCGGTGGGGCGGGGCCCGCCAATGTCTTGAAATAGTTCCGGTTCAGGCCcgcgcgggc                                        >  2:1250054/1‑98 (MQ=255)
                                                               ttgcgccttctccCTGTTGGTTCTTTTGTCCGAGCGGC.GGGCCCGGTGTGCCGGCCCTCGCTAATTTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGAc                                       <  2:2745383/96‑1 (MQ=255)
                                                                ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGGTGGGCCTCGCCCATGTCTTGAAATCGTTCCGGGTCCGGCCCGCGCGCGGCg                                      >  1:3254892/1‑101 (MQ=255)
                                                                ggCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGGGTCCCCCCTGTCTTTGAATAGTTCCCGTTTAGGGCCGCGCCCGGCg                                      >  1:3673933/1‑101 (MQ=255)
                                                                  ctcctcctcCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGGCTCGCCCATGTCTTGAAATCGTTCCGGTTCCGGCCCCCGCGCGACGGc                                    >  2:2475699/1‑101 (MQ=255)
                                                                   tcctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTCAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGcc                                   <  1:3925403/101‑1 (MQ=255)
                                                                   tcctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCCGTGGGCCGGGGCTCGCCAATGTCTTGAAATAGTTCCGGTTCAGGGCCCCGCGCGACGGcc                                   >  1:2675131/1‑101 (MQ=255)
                                                                    cctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGGCCCTCCCATGTCTTTCAATCGCTCCGGGTCCGGGCCCCGCGCGACGGACt                                  >  2:4519582/1‑101 (MQ=255)
                                                                    cctcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGCCTCGCCCATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCCACGGCCt                                  >  1:181659/1‑101 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTc                                <  2:4704507/101‑1 (MQ=255)
                                                                      tcctccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAAGGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTc                                <  2:1498463/101‑1 (MQ=255)
                                                                         tccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGGTCAGGCCCCCGCGCGCCGGGCCTcgcc                             >  1:2924293/1‑100 (MQ=255)
                                                                         tccCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGCCTCGCCCATGTCTTGAAATCGTTCCGGTTCTGGGCCGCGCGCGCCGGCCTTcgcc                             >  1:865238/1‑100 (MQ=255)
                                                                             tgatgaGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCAcc                         <  1:2391611/101‑1 (MQ=255)
                                                                              gatgaGCCTTTCGTCCGAGCGGC.GGGGCCGGGGGGCCGGGCCCCGCCCCAGTCTTGACATCCTTCCAGTTTAAGCCCGCCAGCCACGGAATTGGCGCAccc                        >  1:2944503/1‑100 (MQ=255)
                                                                              gatgaGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCa                        <  2:1336306/101‑1 (MQ=255)
                                                                               atgaGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAg                       <  1:2645893/101‑1 (MQ=255)
                                                                                tgaGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGa                      <  1:2085097/101‑1 (MQ=255)
                                                                                 gaGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGAc                     <  1:3114324/101‑1 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCCATGTCTTTAAATCGTTCCGGTTTAAGCCCCCCCGCGACGGCCTTCGCGCACCAGACGCCCg                >  2:2185327/1‑101 (MQ=255)
                                                                                      tttCGTCCGAGCGGC.GGGCCCCGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTTAGGCCCGCGCGCGACGGCCTTCGCGCCCCCCACGCCCg                >  2:3037076/1‑101 (MQ=255)
                                                                                       ttCGTCCGAGCGGC.GGGCCCGGGGGGGCGGGGCCCGCCAATGTCTTTGAATCGTTCCCGTTCAGGGCCGCGCGCGACGGCCTTCGCGCCCCAATCGCTCgg               >  1:4459177/1‑100 (MQ=255)
                                                                                       ttCGTCCGAGCGGC.GGGCCCGGGGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCCGTTCAGGCCCGCGCGCGACGGCCCTCGCGCACCAGACGCTCGa               >  2:1719711/1‑101 (MQ=255)
                                                                                          gTCCGAGCGGC.GGGCCCGGGGGGCCGGGCCTCGCCAATGTCTTGAAAACGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCCCGGTCa            >  1:4370622/1‑101 (MQ=255)
                                                                                           tCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAg           <  1:1103670/101‑1 (MQ=255)
                                                                                            ccGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgc          <  2:181067/101‑1 (MQ=255)
                                                                                             cGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCCATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGGCTTCGCGCACCCGACGCTCGATCAgcg         >  2:2391062/1‑101 (MQ=255)
                                                                                             cGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcg         >  2:3644780/1‑101 (MQ=255)
                                                                                              gAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCCGACGCTCGCTCAgcgg        >  2:3394870/1‑101 (MQ=255)
                                                                                              gAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCCGACGCCCGATCAgccg        >  1:1211062/1‑99 (MQ=255)
                                                                                                gcggc.gGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCCCCAGACGCCCGATCAgcgggg      >  2:4556427/1‑99 (MQ=255)
                                                                                                gcggc.gGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAgcggcg      <  2:3121244/101‑1 (MQ=255)
                                                                                                  ggc.ggGCCCGGTGGTCCGGGCCTCGCCAATGTCTTGAAGTCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    <  1:3212824/101‑1 (MQ=255)
                                                                                                  ggc.ggGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    >  2:183522/1‑101 (MQ=255)
                                                                                                  ggc.ggGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    >  1:2195591/1‑101 (MQ=255)
                                                                                                  ggc.ggGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    <  1:1456343/101‑1 (MQ=255)
                                                                                                  ggc.ggGCCCGGTGGCCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTc    <  2:1890494/101‑1 (MQ=255)
                                                                                                  ggc.ggGCCCGGGGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCCCCAGACGCTCGATCAGCGGCGTc    <  2:3257287/101‑1 (MQ=255)
                                                                                                   gc.ggGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCa   <  1:1069361/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CCATCGCCAGCGCCCGGATGGCATTGGCCATCCGCGTTTCCTGCGCCATATCGAGATCCTTCATGGCTCCTCCTCCCTGATGAGCCTTTCGTCCGAGCGGC.GGGCCCGGTGGGCCGGGCCTCGCCAATGTCTTGAAATCGTTCCGGTTCAGGCCCGCGCGCGACGGCCTTCGCGCACCAGACGCTCGATCAGCGGCGTCAG  >  NC_014034/1979821‑1980021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG