Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,090 +C 91.2% coding (20/879 nt) potH1 ← polyamine ABC transporter permease PotH

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,0901.→C91.2% 53.0 ‑1.5 ‑0.7217coding (20/879 nt)potH1polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand):  new base (92/106):  ref base (12/7):  total (104/113)
Fisher's exact test strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.86e-01

Only 100 of 225 total aligned reads displayed.

CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CC.ACC.G.C..GGG.T.GGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180
                                                                                                      |                                                                                                 
cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACc.g.c                                                                                                  <  2:2840681/101‑1 (MQ=255)
  acgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACc.g.ccg                                                                                                >  1:2149345/1‑99 (MQ=255)
    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cggg                                                                                            >  1:1197234/1‑100 (MQ=255)
      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cgggtt                                                                                          <  2:3559787/101‑4 (MQ=255)
        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cgggttgg                                                                                        >  1:3229901/1‑96 (MQ=255)
         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cgggttgga                                                                                       <  2:1775910/101‑7 (MQ=255)
         tCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cgggttgga                                                                                       >  1:1930336/1‑95 (MQ=255)
          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cgggttggag                                                                                      >  1:4122779/1‑94 (MQ=255)
           aCCATCGGGGCCGCCACCAACAGAACTGCATAGATCAGCTTTGGGCTGACAATGAGCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.C..cgggttggaga                                                                                     <  2:1879024/101‑9 (MQ=255)
            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGAt                                                                                    <  2:2183062/101‑1 (MQ=255)
             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATg                                                                                   <  1:741401/101‑1 (MQ=255)
              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGc                                                                                  >  2:1124918/1‑101 (MQ=255)
                gggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCg                                                                                <  2:4006969/100‑1 (MQ=255)
                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCg                                                                                >  2:3722926/1‑101 (MQ=255)
                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGAc                                                                              <  1:1111471/101‑1 (MQ=255)
                    gccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACAt                                                                            <  1:4576142/101‑1 (MQ=255)
                    gccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACAt                                                                            <  2:2920667/101‑1 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATAct                                                                         >  2:2365643/1‑101 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATAct                                                                         <  1:2355797/101‑1 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATAct                                                                         <  2:1973744/101‑1 (MQ=255)
                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.G.CCTGGT.T.GGAGATGCCGACATAct                                                                         <  1:2688281/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATActct                                                                       <  1:1625870/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATActct                                                                       <  1:189562/101‑1 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATActct                                                                       >  2:3689726/1‑101 (MQ=255)
                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATActct                                                                       <  1:3180907/101‑1 (MQ=255)
                          aGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTa                                                                      <  1:4023976/101‑1 (MQ=255)
                          aGCAACAGAACGGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTa                                                                      <  2:4517130/101‑1 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAt                                                                     <  2:2087972/101‑1 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAt                                                                     <  1:2048712/101‑1 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAt                                                                     >  2:4294092/1‑101 (MQ=255)
                           gCAACAGAACTGCATAGATCAGCGTTGGGCTGACCAGGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAt                                                                     <  2:443403/101‑1 (MQ=255)
                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAtt                                                                    <  1:1449539/101‑1 (MQ=255)
                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAtt                                                                    >  2:1827147/1‑101 (MQ=255)
                            cAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTAtt                                                                    <  2:694604/101‑1 (MQ=255)
                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATtc                                                                   >  2:2720612/1‑101 (MQ=255)
                                aGACCTGCATAGATCGGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTct.cc                                                               <  1:923883/101‑1 (MQ=255)
                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTct.cc                                                               >  2:3849453/1‑101 (MQ=255)
                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTct.cc                                                               >  1:956613/1‑101 (MQ=255)
                                 gAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTct.ccc                                                              >  1:1497511/1‑100 (MQ=255)
                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTct.ccct                                                             >  2:3294362/1‑99 (MQ=255)
                                   aCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTct.ccctc                                                            >  1:4249532/1‑98 (MQ=255)
                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          <  2:2149221/101‑1 (MQ=255)
                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAgc                                                          >  1:2888960/1‑101 (MQ=255)
                                             gcAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   <  1:3465650/100‑6 (MQ=255)
                                             gcAGCGTGGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAgcgcgtctc                                                   <  2:2312407/100‑6 (MQ=255)
                                              cAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCa                                                  <  1:2344181/101‑1 (MQ=255)
                                               aGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.g                                                <  1:4433421/101‑1 (MQ=255)
                                                 cGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCGGCGCGTCTCA.ggc                                              >  1:4080034/1‑99 (MQ=255)
                                                  gTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcg                                             >  1:4319596/1‑98 (MQ=255)
                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCA.ggcgcg                                           >  2:1955312/1‑96 (MQ=255)
                                                       gCTGCCCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  1:1259870/101‑1 (MQ=255)
                                                       gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGttt                                         <  2:2450949/101‑1 (MQ=255)
                                                        cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCAACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGCTTg                                        >  2:484784/1‑101 (MQ=255)
                                                         tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTgg                                       >  2:2980315/1‑101 (MQ=255)
                                                          gACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTggg                                      >  2:1894295/1‑101 (MQ=255)
                                                           aCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGc                                     >  1:1240486/1‑101 (MQ=255)
                                                            cgatcgacatgCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGcc                                    >  2:3982508/11‑101 (MQ=255)
                                                              aTGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  <  1:673138/101‑1 (MQ=255)
                                                              aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaa                                  >  1:3257950/1‑101 (MQ=255)
                                                               tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCaaa                                 >  2:1051565/1‑101 (MQ=255)
                                                                  cccAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              <  2:1064900/100‑1 (MQ=255)
                                                                  aCCAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAAttt                              >  1:3670697/1‑101 (MQ=255)
                                                                   ccAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTg                             >  1:1774148/1‑101 (MQ=255)
                                                                    cAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGc                            >  2:4159853/1‑101 (MQ=255)
                                                                    cAGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGc                            >  1:3262616/1‑101 (MQ=255)
                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           >  2:4087531/1‑101 (MQ=255)
                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           <  2:2245066/101‑1 (MQ=255)
                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGca                           >  1:188619/1‑101 (MQ=255)
                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTCca                           >  1:4684440/1‑101 (MQ=255)
                                                                       ccGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                         <  2:4242580/101‑1 (MQ=255)
                                                                       ccGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                         <  2:1497410/101‑1 (MQ=255)
                                                                        cGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                        <  2:4249285/101‑1 (MQ=255)
                                                                        cGCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACaa                        <  1:3752396/101‑1 (MQ=255)
                                                                         gCTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  2:2180500/101‑1 (MQ=255)
                                                                         ccgggCCGCCTCCCAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCAACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                       <  2:4716753/98‑1 (MQ=255)
                                                                          cTGGCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAgg                      <  2:3759767/101‑1 (MQ=255)
                                                                           tgaccgccTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGt                     >  1:2497391/4‑101 (MQ=255)
                                                                            ggCCGCCTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                    >  1:1017072/1‑101 (MQ=255)
                                                                              ccgccTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  1:3868112/101‑1 (MQ=255)
                                                                              ccgccTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  >  2:629880/1‑101 (MQ=255)
                                                                              ccgccTCCGAGCGCGCCC.ACC.GCC..GGG.TGGGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  >  1:1854555/1‑101 (MQ=255)
                                                                              cccccTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                  <  2:3216351/98‑1 (MQ=255)
                                                                               cgccTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCg                 >  1:1615796/1‑101 (MQ=255)
                                                                                 ccTCCGAGCGCGCCC.ACCTC.C..GGGTT.GGAGATGCCTACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc               <  2:3577525/101‑1 (MQ=255)
                                                                                 ccTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc               >  1:3951464/1‑101 (MQ=255)
                                                                                  cTCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa              >  2:2613097/1‑101 (MQ=255)
                                                                                   tCCGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag             <  2:1532472/101‑1 (MQ=255)
                                                                                     cGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag           <  2:1609359/101‑1 (MQ=255)
                                                                                     cGAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag           >  2:3024968/1‑101 (MQ=255)
                                                                                      gAGCGCGCCC.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg          <  2:1369972/101‑1 (MQ=255)
                                                                                         ggcgccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  2:4586691/96‑1 (MQ=255)
                                                                                         cgcgccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:2980503/96‑1 (MQ=255)
                                                                                         cgcgccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:1057655/96‑1 (MQ=255)
                                                                                         cgcgccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa       <  1:1344280/96‑1 (MQ=255)
                                                                                          gcgccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAg      <  2:404323/97‑1 (MQ=255)
                                                                                            gccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggg    >  1:1036868/3‑101 (MQ=255)
                                                                                             ccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg   >  1:2230258/2‑101 (MQ=255)
                                                                                             ccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg   >  2:1444238/2‑101 (MQ=255)
                                                                                             ccc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg   <  2:3917546/100‑1 (MQ=255)
                                                                                              cc.ACC.GCC..GGGTT.GGAGATGCCGACATACTCTATTCTCCCTCAGCGCGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg  <  1:2520222/101‑1 (MQ=255)
                                                                                                      |                                                                                                 
CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CC.ACC.G.C..GGG.T.GGAGATGCCGACATACTCTATTCT.CCTCAGCGC.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGG  >  NC_014034/2046992‑2047180

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG