Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,047,128 +G 100% intergenic (‑19/+149) potH1 ← / ← RCAP_rcc01896 polyamine ABC transporter permease PotH/GntR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,047,1281.→G100.0% 760.7 200intergenic (‑19/+149)potH1/RCAP_rcc01896polyamine ABC transporter permease PotH/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (95/105):  ref base (0/0):  total (95/105)

Only 100 of 219 total aligned reads displayed.

ATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTA.TTC.T.CCTCAGCGC.TCTC.A.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGC  >  NC_014034/2047032‑2047221
                                                                                                       |                                                                                               
aTAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAgcgc                                                                                                  >  2:3713258/1‑101 (MQ=255)
    aTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTCGC.CCTCAgcgcgtct                                                                                              >  1:4097629/1‑97 (MQ=255)
    aTCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAgcgcgtct                                                                                              >  2:634236/1‑97 (MQ=255)
     gcAGCGTGGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAgcgcgtctc                                                                                             <  2:2312407/100‑6 (MQ=255)
          gTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGGGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.CTC.TCCCTCACCGCGTCTC.A.ggcg                                                                                      >  2:4650784/1‑98 (MQ=255)
          gTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.A.ggcg                                                                                      >  1:4319596/1‑98 (MQ=255)
              ggCTGCCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGtt                                                                                   <  2:2352032/101‑1 (MQ=255)
               gCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGttt                                                                                  <  2:2450949/101‑1 (MQ=255)
                cTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTg                                                                                 >  1:618565/1‑101 (MQ=255)
                 tGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTgg                                                                                >  2:1004048/1‑101 (MQ=255)
                  gcccATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTggg                                                                               <  1:1619389/99‑1 (MQ=255)
                  gACCATGAACCAGCAGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.ACGCGCGTTTgag                                                                               >  2:4736650/1‑99 (MQ=255)
                   aCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGc                                                                              >  1:1240486/1‑101 (MQ=255)
                    cgatcgacatgCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGcc                                                                             >  2:3982508/11‑101 (MQ=255)
                      aTGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCaa                                                                           <  1:673138/101‑1 (MQ=255)
                      aTGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCaa                                                                           <  2:1955522/101‑1 (MQ=255)
                       tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCaaa                                                                          >  2:1051565/1‑101 (MQ=255)
                       tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCaaa                                                                          >  2:4159778/1‑101 (MQ=255)
                        gAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAAt                                                                         <  1:2329273/101‑1 (MQ=255)
                          aCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAAttt                                                                       <  1:1096420/101‑1 (MQ=255)
                           ccAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTg                                                                      >  1:1774148/1‑101 (MQ=255)
                            cAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGc                                                                     >  1:3262616/1‑101 (MQ=255)
                             aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGca                                                                    <  2:2245066/101‑1 (MQ=255)
                                cGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACaa                                                                 <  1:3752396/101‑1 (MQ=255)
                                 gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAg                                                                <  2:2180500/101‑1 (MQ=255)
                                  cTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAgg                                                               <  2:3759767/101‑1 (MQ=255)
                                   tgaccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                              >  1:2497391/4‑101 (MQ=255)
                                   tGGCCGCCTGCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                              <  2:625372/101‑1 (MQ=255)
                                   tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                              >  2:1809406/1‑101 (MQ=255)
                                   tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                              >  2:1721987/1‑101 (MQ=255)
                                   tGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGt                                                              >  2:2520077/1‑101 (MQ=255)
                                    ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGtt                                                             >  1:1017072/1‑101 (MQ=255)
                                      ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                                                           <  1:3868112/101‑1 (MQ=255)
                                      ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                                                           >  1:1049625/1‑101 (MQ=255)
                                        gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg                                                         >  1:1889624/1‑101 (MQ=255)
                                         ccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                                        >  1:3951464/1‑101 (MQ=255)
                                         ccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGc                                                        <  2:1867464/101‑1 (MQ=255)
                                          cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa                                                       >  2:2613097/1‑101 (MQ=255)
                                          cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa                                                       >  1:2462743/1‑101 (MQ=255)
                                           tCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag                                                      <  2:1532472/101‑1 (MQ=255)
                                            ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga                                                     >  1:1825509/1‑101 (MQ=255)
                                             cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag                                                    <  2:1609359/101‑1 (MQ=255)
                                             cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag                                                    >  2:3024968/1‑101 (MQ=255)
                                              gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg                                                   <  2:1369972/101‑1 (MQ=255)
                                              gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg                                                   >  2:3857873/1‑101 (MQ=255)
                                              gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg                                                   <  2:4080144/101‑1 (MQ=255)
                                                tcctgcccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaa                                                 >  2:193449/7‑101 (MQ=255)
                                                 ggcgcccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa                                                <  2:4586691/96‑1 (MQ=255)
                                                 cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa                                                <  1:360542/96‑1 (MQ=255)
                                                 cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa                                                <  2:4244626/96‑1 (MQ=255)
                                                 cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa                                                <  1:4651040/96‑1 (MQ=255)
                                                     cccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg                                            >  1:2230258/2‑101 (MQ=255)
                                                     cccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAgggg                                            >  2:1444238/2‑101 (MQ=255)
                                                      ccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg                                           >  1:1949560/1‑101 (MQ=255)
                                                      ccACCGCCGGGTTGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAggggg                                           <  1:2520222/101‑1 (MQ=255)
                                                        cccgccgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGAc                                         <  1:3905285/91‑1 (MQ=255)
                                                        accgccgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGAc                                         <  2:3232382/91‑1 (MQ=255)
                                                        accgccgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGAc                                         <  1:1882772/91‑1 (MQ=255)
                                                        accgccgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGAc                                         <  1:3256195/91‑1 (MQ=255)
                                                           gccgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGAt                                      <  1:2760402/94‑1 (MQ=255)
                                                            ccgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATg                                     <  2:2740426/95‑1 (MQ=255)
                                                             cgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGc                                    <  1:2992802/96‑1 (MQ=255)
                                                             cgggttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTCGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGc                                    <  2:4101126/96‑1 (MQ=255)
                                                               ggttGGANATGCCNACNNACTCNANTNN.N.NNTCAGCGCGTCTC.AGGCGCGTTNGGGCCANATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGccc                                  <  1:634300/98‑1 (MQ=255)
                                                                gttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                                 <  1:1264996/99‑1 (MQ=255)
                                                                gttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGcccc                                 >  2:4212805/3‑101 (MQ=255)
                                                                 ttGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCg                                >  2:1259959/2‑101 (MQ=255)
                                                                  tGGAGATGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGCCGATGCCCCgg                               >  2:3403777/1‑101 (MQ=255)
                                                                    gagaTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                             <  1:193474/101‑1 (MQ=255)
                                                                    gagaTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                             <  2:4036368/101‑1 (MQ=255)
                                                                    gagaTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                             >  1:3099113/1‑101 (MQ=255)
                                                                    gagaTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCt                             >  1:3366810/1‑101 (MQ=255)
                                                                     agaTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCtt                            <  1:4274579/101‑1 (MQ=255)
                                                                      gaTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                           >  1:4151861/1‑101 (MQ=255)
                                                                      gaTGCCGACATACTCTA.TGC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                           <  2:3190438/101‑1 (MQ=255)
                                                                      agtGCCTACATACTCTA.TTC.TCCCTCAGCACGTCGC.AGGCGCGTTCGGGCCGAACTTGCAAAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTg                           <  2:1148435/99‑1 (MQ=255)
                                                                       aTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGTAAGGGGGACGATGCCCCGGCTTGc                          >  2:2656336/1‑101 (MQ=255)
                                                                       aTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGc                          <  2:3098913/101‑1 (MQ=255)
                                                                       aTGCCGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGc                          >  2:4616459/1‑101 (MQ=255)
                                                                           cGACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGcc                      <  2:1914298/101‑1 (MQ=255)
                                                                            gACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCt                     >  2:503492/1‑101 (MQ=255)
                                                                            gACATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGAGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCt                     <  1:4044674/101‑1 (MQ=255)
                                                                              cATACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGt                   >  1:3562433/1‑101 (MQ=255)
                                                                               aTACTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGtt                  <  1:3034880/101‑1 (MQ=255)
                                                                                tACTCTA.TTC.TCCCTCAACGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGttt                 <  2:4267939/101‑1 (MQ=255)
                                                                                 aCTCTA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTc                >  2:1887705/1‑101 (MQ=255)
                                                                                  ctctA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCg               >  1:3854318/1‑101 (MQ=255)
                                                                                   tctA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCgg              >  1:964967/1‑101 (MQ=255)
                                                                                    ctA.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCggg             >  1:2912218/1‑101 (MQ=255)
                                                                                     tA.TTC.TCCCTCAGCGCGTCTCGT.GCGCGTTTGGGCCAAACTTGCAAAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGTCTGTTTCGGGa            <  2:2915856/101‑1 (MQ=255)
                                                                                      a.TTC.TCCCTCAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaa           >  1:482885/1‑101 (MQ=255)
                                                                                         ttctccctcAGCGCGCCTC.AGGCGTGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGaaa          <  1:717446/96‑1 (MQ=255)
                                                                                          tctccctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAAt         <  1:2197897/97‑1 (MQ=255)
                                                                                           ctccctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAAtt        >  1:2407883/4‑101 (MQ=255)
                                                                                            tccctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAAttt       >  1:2666111/3‑101 (MQ=255)
                                                                                             ccctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTc      <  2:3562214/100‑1 (MQ=255)
                                                                                             ccctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTc      <  1:2365776/100‑1 (MQ=255)
                                                                                              cctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTcc     <  2:691064/101‑1 (MQ=255)
                                                                                               ctcAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTccc    <  2:3670468/101‑1 (MQ=255)
                                                                                                 cAGCGCGTCTC.AGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCcgc  >  1:1497518/1‑101 (MQ=255)
                                                                                                       |                                                                                               
ATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTA.TTC.T.CCTCAGCGC.TCTC.A.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGGGGACGATGCCCCGGCTTGCGGCCTGTTTCGGGAAATTTCCCGC  >  NC_014034/2047032‑2047221

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG