Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,825 +C 85.9% intergenic (‑591/‑311) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8251.→C85.9% 80.4 ‑2.9 ‑1.4184intergenic (‑591/‑311)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (76/82):  ref base (18/8):  total (94/90)
Fisher's exact test strand distribution p-value = 5.70e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.21e-02

Only 100 of 206 total aligned reads displayed.

AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCG.CG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913
                                                                                                     |                                                                                             
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                >  2:1829171/1‑101 (MQ=255)
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                <  2:1909143/101‑1 (MQ=255)
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                >  2:4108386/1‑101 (MQ=255)
aaaaTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAt                                                                                                >  2:3950116/1‑101 (MQ=255)
   aTTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.ccc                                                                                            <  2:1880773/101‑2 (MQ=255)
    tttGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccg                                                                                           >  2:2185167/1‑99 (MQ=255)
      tGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.cccgcg                                                                                         >  1:4504176/1‑97 (MQ=255)
        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc..cg.cgcc                                                                                    >  1:1263749/1‑100 (MQ=255)
        tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc..cg.cgcc                                                                                    <  1:131149/101‑2 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGca                                                                                >  1:2186292/1‑100 (MQ=255)
           tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGca                                                                                >  1:1134199/1‑100 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaag                                                                              >  2:2124151/1‑98 (MQ=255)
             tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaag                                                                              >  2:4684035/1‑98 (MQ=255)
              ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaagc                                                                             >  2:2893933/1‑97 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACACT.CC..CG.CGC.CGcaagcg                                                                            >  2:239297/1‑96 (MQ=255)
               tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaagcg                                                                            >  1:3723150/1‑96 (MQ=255)
                tccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaagcgt                                                                           <  2:1317534/100‑7 (MQ=255)
                 ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaagcgtt                                                                          <  2:3437825/101‑8 (MQ=255)
                  cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGCCAAT.CC..CG.CGC.CGcaagcgttt                                                                         >  1:2117822/1‑93 (MQ=255)
                  cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaagcgttt                                                                         >  2:3587988/1‑93 (MQ=255)
                   gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC..CG.CGC.CGcaagcgtttc                                                                        >  2:2756367/1‑92 (MQ=255)
                          aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCg                                                                     >  1:1775216/1‑101 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGc                                                                   <  1:2835799/101‑1 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGc                                                                   >  2:1445076/1‑101 (MQ=255)
                            cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGc                                                                   <  1:1642245/101‑1 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCAt                                                                 >  1:3070863/1‑101 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:1134113/101‑1 (MQ=255)
                              cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCAt                                                                 <  2:476038/101‑1 (MQ=255)
                               tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATc                                                                <  2:3338291/101‑1 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCa                                                               <  1:883138/101‑1 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCa                                                               >  2:3128791/1‑101 (MQ=255)
                                gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCa                                                               <  1:4449913/101‑1 (MQ=255)
                                 cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCat                                                              <  2:3929740/101‑2 (MQ=255)
                                  ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCatt                                                             <  2:1797649/101‑3 (MQ=255)
                                   tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                            <  2:2573330/101‑1 (MQ=255)
                                   tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTc                                                            >  1:3235399/1‑101 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  1:1043175/1‑101 (MQ=255)
                                    cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCt                                                           >  1:824865/1‑101 (MQ=255)
                                     ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCtt                                                          <  1:392945/101‑1 (MQ=255)
                                      gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTg                                                         <  1:2784003/101‑1 (MQ=255)
                                       cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTgg                                                        <  2:3657945/101‑1 (MQ=255)
                                       cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTgg                                                        <  2:4030698/101‑1 (MQ=255)
                                        gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.c                                                      >  2:571394/1‑101 (MQ=255)
                                         cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  1:2124272/101‑1 (MQ=255)
                                          gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                    >  1:4585884/1‑101 (MQ=255)
                                          gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccc                                                    <  2:3561699/101‑1 (MQ=255)
                                           cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccc                                                   <  2:2283930/101‑1 (MQ=255)
                                             gccTTCGGGGCGGCGGCGGGTCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCAGCATCATTCTTGG.cccccc                                                 <  1:3446930/99‑2 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:534561/101‑2 (MQ=255)
                                             gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:2614122/101‑2 (MQ=255)
                                                ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              <  2:846428/101‑5 (MQ=255)
                                                 tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  1:297722/101‑6 (MQ=255)
                                                 tCGGGGCGGCGGCGGGCCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  2:3329062/101‑6 (MQ=255)
                                                  cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCgg                                             <  1:4750815/101‑1 (MQ=255)
                                                   ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            <  2:4692896/101‑1 (MQ=255)
                                                   ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCggg                                            >  1:4707809/1‑101 (MQ=255)
                                                      gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACa                                         <  1:635911/101‑1 (MQ=255)
                                                          cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  2:15036/101‑1 (MQ=255)
                                                           ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:1290873/101‑1 (MQ=255)
                                                              ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  2:3239815/1‑101 (MQ=255)
                                                                ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  1:1947064/1‑101 (MQ=255)
                                                                   tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:1185803/1‑101 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:398826/1‑101 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:2918187/1‑101 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  1:3138544/101‑1 (MQ=255)
                                                                    ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  1:3588207/101‑1 (MQ=255)
                                                                     ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:1086702/101‑1 (MQ=255)
                                                                     ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  2:519427/101‑1 (MQ=255)
                                                                     ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:2025462/101‑1 (MQ=255)
                                                                     ttGCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:4266222/101‑1 (MQ=255)
                                                                       gCGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAtt                        >  1:162260/1‑101 (MQ=255)
                                                                        cGATCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:4287722/101‑1 (MQ=255)
                                                                          aTCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     >  2:1354625/1‑101 (MQ=255)
                                                                          aTCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  1:4638308/101‑1 (MQ=255)
                                                                           tCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3736562/1‑101 (MQ=255)
                                                                           tCCTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3837664/1‑101 (MQ=255)
                                                                            ccTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  2:1336364/101‑1 (MQ=255)
                                                                            ccTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   <  1:1713669/101‑1 (MQ=255)
                                                                             cTGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  1:1524027/1‑101 (MQ=255)
                                                                              tGCGTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:21431/1‑101 (MQ=255)
                                                                              ggCGTTTCCGGATTGGCCACAATCCCGCCC.CGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 <  1:2875507/100‑1 (MQ=255)
                                                                                 gTTTCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:4381347/1‑101 (MQ=255)
                                                                                  tttCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:4462882/1‑101 (MQ=255)
                                                                                   ttCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:4480853/1‑101 (MQ=255)
                                                                                   ttCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:3723001/1‑101 (MQ=255)
                                                                                   ttCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            >  2:3085795/1‑101 (MQ=255)
                                                                                    tCCGGATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           >  2:3845429/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:2953626/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        <  2:462074/101‑1 (MQ=255)
                                                                                       ggATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:755938/1‑101 (MQ=255)
                                                                                       ggATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:2112568/1‑101 (MQ=255)
                                                                                        gATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  1:3480609/1‑101 (MQ=255)
                                                                                        gATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:546236/101‑1 (MQ=255)
                                                                                        gATTGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  1:1188528/1‑101 (MQ=255)
                                                                                          ttGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:583761/101‑1 (MQ=255)
                                                                                          ttGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  1:3311679/1‑101 (MQ=255)
                                                                                          ttGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTCCGATCaa     >  2:3063622/1‑101 (MQ=255)
                                                                                           tGGCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  2:3774145/1‑101 (MQ=255)
                                                                                            ggCGACAATCCCG.CGCCGCAAGCGTTTCCGAACGGGCATCATTCTTGGCCCCCCGCCGGGACAATCCAGGCCGCCGCATTGTTCCGCCATTGCGATCaacg   >  2:2021894/1‑99 (MQ=255)
                                                                                             gCGACAATCCCG.CGCCGCAAGCGTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  >  2:2811096/1‑101 (MQ=255)
                                                                                                     |                                                                                             
AAAATTTGTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCG.CG.CGC.AGCG.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057725‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG